Project/Area Number |
11660314
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied veterinary science
|
Research Institution | Miyazaki University |
Principal Investigator |
HAGIO Mitsuyoshi Miyazaki Univ., Department of Veterinary Science, Professor, 農学部, 教授 (20128359)
|
Co-Investigator(Kenkyū-buntansha) |
MIYAMOTO Toru Miyazaki Univ., Department of Veterinary Medicine, Associate Prof., 農学部, 助手 (40315362)
|
Project Period (FY) |
1999 – 2000
|
Project Status |
Completed (Fiscal Year 2000)
|
Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
Keywords | Ultrasonography / uterus and ovary / estrous cycle / Japanese Black cow |
Research Abstract |
The objectives of this study were to determine the optimal tomoraphic planes to use for an adequate transrectal Doppler examination of the uterine and ovarian blood flows of Japanese Black cows, and to evaluate changes in their blood flow patterns during the estrous cycle. The uterine artery could be identified by comparing the presumed uterine artery of non-pregnant cow with those of pregnant cows. With the complementary use of color Doppler, the uterine artery could be readily located and an optimal angle for insonation with pulsed Doppler could be provided in 8 of 10 cows. Uterine arterial velocity waveforms had some characteristic features such that during diestrous, there was a systolic waveform with rapid rise and fall in velocity, followed by a diastolic waveform with a relatively low speed, whereas during estrous, a diastolic waveform had higher speed than that of during diestrous in spite of a similar systolic waveform as seen during diestrous. The resistance index(RI) as a quantitative index for characterizing waveforms also varied regularly during the estrous cycle. The RI calues began to decrease from around 4 day before estrous(Day 0) to reach the lowest between Day -3 and -1, then increased gradually or rapidly. As for the blood flows in the ovary, color Doppler blood flow signals surrounding and within the corpus luteum on could be seen from the early luteal phase of the estrous cycle, reached the peak of vascularity at functional luteal stage, and then diminished its vascularity to almost vanish 3 day before ovalation. These data suggest that a noninvasive Doppler ultrasonography could be developed for detecting stage of the estrous cycle by evaluating differences in Doppler velocity waveforms and RI values of the uterine artery as well as vascularity of the corpus luteum.
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