がん細胞の分化・極性の異常と細胞極性シグナリング

• 研究課題/領域番号: 17014076
• 研究種目: 特定領域研究
• 配分区分: 補助金
• 審査区分: 生物系
• 研究機関: 横浜市立大学
• 研究代表者: 大野 茂男 横浜市立大学, 医学研究科, 教授 (10142027)
• 研究分担者: 鈴木 厚 横浜市立大学, 大学院・医学研究科, 准教授 (00264606) ; 秋本 和憲 横浜市立大学, 医学部, 助教 (70285104) ; 廣瀬 智威 横浜市立大学, 医学部, 助教 (20381668) ; 水野 恵子 横浜市立大学, 医学部, 助手 (90221803)
• 研究期間 (年度): 2005 – 2009
• 研究課題ステータス: 完了 (2009年度)
• 配分額: 328,800千円 (直接経費: 328,800千円); 2009年度: 66,100千円 (直接経費: 66,100千円); 2008年度: 66,100千円 (直接経費: 66,100千円); 2007年度: 66,300千円 (直接経費: 66,300千円); 2006年度: 66,300千円 (直接経費: 66,300千円); 2005年度: 64,000千円 (直接経費: 64,000千円)
• キーワード: aPKC / MDCK細胞 / PAR-3 / PAR-6 / 上皮細胞 / 前立腺がん / 胃がん / 乳がん / ASIP / 線虫 / 非対称分裂 / 極性 / 上皮

研究概要

幹細胞の非対称分裂や上皮の極性を普遍的に制御する細胞極性の制御因子に着目した研究を行い、(1)aPKC-PAR複合体の下流因子PAR1によるUtrophin/Dystroglycan複合体を介した細胞外基質の制御の発見、p53の細胞死誘導能を担うASPP2の新たな役割として細胞極性制御を同定した。(2)乳腺組織幹前駆細胞の増殖制御機構としてaPKC-ErbB2 negative feedback経路を発見した。(3)前立腺がん、胃がんなどでのaPKCとがんの悪性化との関わり、前立腺がんの再燃におけるaPKC-EL6経路によるオートクライン増殖機構を見いだした。

研究成果

• [雑誌論文] AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK family and function in nonsense-mediated mRNA decay. (2010)
  • 著者名/発表者名: Izumi N, Yamashita A, Iwamatsu A, Kurata R, Nakamura H, Saari B, Hirano H, Anderson P, Ohno S
  • 雑誌名: Science Signaling 3(116)
• [雑誌論文] AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK family and function in nonsense-mediated Mrna decay. (2010)
  • 著者名/発表者名: Inumi N, Yamashita A, Iwamatsu A, Kurata R, Nakamura H, Saari B, Hirano H, Anderson P, Ohno S
  • 雑誌名: Science Signaling 3(116)
  • 査読あり
• [雑誌論文] A cell polarity protein aPKClambda is required for eye lens formation and growth. (2009)
  • 著者名/発表者名: Sugiyama Y, Akimoto K, Robinson M L, Ohno S, Quinlan R A
  • 雑誌名: Dev Biol 336(2) ページ: 246-256
• [雑誌論文] High expression of atypical protein kinase C lambda/iota in gastric cancer as a prognostic factor for recurrence. (2009)
  • 著者名/発表者名: Takagawa R, Akimoto K, Ichikawa Y, Akiyama H, Kojima Y, Ishiguro H, Inayama Y, Aoki I, Kunisaki C, Endo I, Nagashima Y, Ohno S
  • 雑誌名: Ann Surg Oncol 17(1) ページ: 81-88
• [雑誌論文] aPKClambda/iota promotes growth of prostate cancer cells in an autocrine manner through transcriptional activation of interleukin-6. (2009)
  • 著者名/発表者名: Ishiguro H, Akimoto K, Nagashima Y, Kojima Y, Sasaki T, Ishiguro-Imagawa Y, Nakaigawa N, Ohno S, Kubota Y, Uemura H
  • 雑誌名: Proc Natl Acad Sci U S A 106(38) ページ: 16369-16374
• [雑誌論文] An essential role of the aPKC-Aurora A-NDEL1 pathway in neurite elongation by modulation of microtubule dynamics. (2009)
  • 著者名/発表者名: Mori D, Yamada M, Mimori-Kiyosue Y, Shirai Y, Suzuki A, Ohno S, Saya H, Wynshaw-Boris A, Hirotsune S
  • 雑誌名: Nat Cell Biol 11(9) ページ: 1057-1068
• [雑誌論文] Intracellular polarity protein PAR-1 regulates extracellular laminin assembly by regulating the dystroglycan complex. (2009)
  • 著者名/発表者名: Masuda-Hirata M, Suzuki A, Amano Y, Yamashita K, Ide M, Yamanaka T, Sakai M, Imamura M, Ohno S
  • 雑誌名: Genes Cells 14(7) ページ: 835-850
• [雑誌論文] Interaction between PAR-3 and the aPKC-PAR-6 complex is indispensable for apical domain development of epithelial cells. (2009)
  • 著者名/発表者名: Horikoshi Y, Suzuki A, Yamanaka T, Sasaki K, Mizuno K, Sawada H, Yonemura S, Ohno S
  • 雑誌名: J. Cell Science 122(10) ページ: 1595-1606
• [雑誌論文] SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay. (2009)
  • 著者名/発表者名: Yamashita A, Izumi N, Kashima I, Ohnishi T, Saari B, Katsuhata Y, Muramatsu R, Morita T, Iwamatsu A, Hachiya T, Kurata R, Hirano H, Anderson P, Ohno S
  • 雑誌名: Genes Dev. 23(9) ページ: 1091-1105
• [雑誌論文] An essential role of the universal polarity protein, aPKClambda, on the maintenance of podocyte slit diaphragms. (2009)
  • 著者名/発表者名: Hirose T, Satoh D, Kurihara H, Kusaka C, Hirose H, Akimoto K, Matsusaka T, Ichikawa I, Noda T, Ohno S
  • 雑誌名: PLoS ONE 4(1)
• [雑誌論文] The 8th and 9th tandem spectrin-like repeats of utrophin cooperatively form a functional unit to interact with polarity-regulating kinase PAR-1b. (2009)
  • 著者名/発表者名: Yamashita K, Suzuki a, Satoh Y, Ide M, Amano Y, Masuda-Hirata M, Hayasi Y.K, Hamada K, Ogata K, Ohno S
  • 雑誌名: Biochem Biophys Res.Commun 391(1) ページ: 812-817
  • 査読あり
• [雑誌論文] A cell polarity protein aPKC1ambda is required for eye lens formation and growth. (2009)
  • 著者名/発表者名: Sugiyama Y, Akimoto K, Robinson M.L, Ohno S, Qinlan R.A
  • 雑誌名: Dev Biol 336(2) ページ: 246-256
  • 査読あり
• [雑誌論文] High expression of atypical protein kinase C lambda/iota in gastric cancer as aprognostic factor for recurrence. (2009)
  • 著者名/発表者名: Takagawa R, Akimoto K, Ichikawa Y, Akiyama H, Kojima Y, Ishiguro H, Inayama Y, Aoki I, Kunisaki C, Endo I, Ngashima Y, Ohno S
  • 雑誌名: Ann Surg Oncol 17(1) ページ: 81-88
  • 査読あり
• [雑誌論文] aPKClambda/iota promotes growth of prostate cancer cells in an autocrine manner through transcriptional activation of interleukin-6. (2009)
  • 著者名/発表者名: Ishiguro H, Akimoto K, Nagashima Y, Kojima Y, Sasaki T, Ishiguro-Imagawa Y, Nakaigawa N, Ohno S, Kubota Y, Uemura H
  • 雑誌名: Proc Natl Acad Sci USA 106(38) ページ: 16369-16374
  • 査読あり
• [雑誌論文] An essential role of the aPKC-Aurora A-NDEL1 pathway in neurite elongationby modulation of microtubule dynamics. (2009)
  • 著者名/発表者名: Mori D, Yamada M, Mimori-Kiyosue Y, Shirai Y, Suzuki A, Ohno S, Saya H, Wynshaw-Boris A, Hirotsune S
  • 雑誌名: Nat Cell Biol 11(9) ページ: 1057-1068
  • 査読あり
• [雑誌論文] Interaction between PAR-3 and the aPKC-PAR-6 complex is indispensable for apical domain development of epithelial cells. (2009)
  • 著者名/発表者名: Horikoshi Y, Suzuki A, Yamanaka T, Sasaki K, Mizuno K, Sawada H, Yonmura S, Ohno S
  • 雑誌名: Journal of Cell Science 122(10) ページ: 1595-1606
  • 査読あり
• [雑誌論文] SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated Mrna decay. (2009)
  • 著者名/発表者名: Yamashita A, Izumi N, Kashima I, Ohnishi T, Saari B, Katsuhata Y, Muramatsu R, Morita T, Iwamatsu A, Hachiya T, Kurata R, Hirano H, Anderson P, Ohno S
  • 雑誌名: Genes & Development 23(9) ページ: 1091-1105
  • 査読あり
• [雑誌論文] An essential role of the universal polarity protein, aPKC1ambda, on the maintenance of podocyte slit diaphragms. (2009)
  • 著者名/発表者名: Hirose T, Satoh D, Kurihara H, Kusaka C, Hirose H, Akimoto K, Matsusaka T, Ichikawa I, Noda T, Ohno S
  • 雑誌名: PLoS ONE 4(1) ページ: 4194-4194
  • 査読あり
• [雑誌論文] Intracellular polarity protein PAR-1 regulates extracellular laminin assembly by regulating the dystroglycan Complex (2009)
  • 著者名/発表者名: Masuda-Hirata M, Suzuki A, Amano Y, Yamashita K, Ide M, Yamanaka T, Sakai M, Imamura Mand Ohno S
  • 雑誌名: Genes to Cells (in press)
  • 査読あり
• [雑誌論文] SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay (2009)
  • 著者名/発表者名: Yamashita A, Izumi N, Kashima I, Ohnishi T, Saari B, Katsuhata Y, Muramatsu R, Morita T, Iwamatsu A, Hachiya T, Kurata R, Hirano H, Anderson P, Ohno S
  • 雑誌名: Genes & Development 23(9) ページ: 1091-1105
  • 査読あり
• [雑誌論文] Interaction between PAR-3 and the aPKC-PAR-6 complex is indispensable for apical domain development of epithelial cells (2009)
  • 著者名/発表者名: Horikoshi Y, Suzuki A, Yamanaka T, Sasaki K, Mizuno K, Sawada H, Yonemura S, Ohno S
  • 雑誌名: Journal of Cell Science 122(10) ページ: 1595-1606
  • 査読あり
• [雑誌論文] An essential role of the universal polarity protein, aPKClambda, on the maintenance of podocyte slit diaphragms (2009)
  • 著者名/発表者名: Hirose T, Satoh D, KuriharaH, Kusaka C, Hirose H, Akimoto K, Matsusaka T, Ichikawa I, Noda T, Ohno S
  • 雑誌名: PLoS ONE 4(1)
  • 査読あり
• [雑誌論文] aPKC enables development of zonula adherens by antagonizing centripetal contraction of the circumferential actomyosin cables. (2008)
  • 著者名/発表者名: Kishikawa M, Suzuki A, Ohno S
  • 雑誌名: J Cell Sci 121(Pt15) ページ: 2481-2492
• [雑誌論文] The over- expression and altered localization of the atypical protein kinase C lambda/iota in breast cancer correlates with the pathologic type of these tumors. (2008)
  • 著者名/発表者名: Kojima Y, Akimoto K, Nagashima Y, Ishiguro H, Shirai S, Chishima T, Ichikawa Y, Ishikawa T, Sasaki T, Kubota Y, Inayama Y, Aoki I, Ohno S, Shimada H
  • 雑誌名: Hum Pathol 39(6) ページ: 824-831
• [雑誌論文] Role of Lgl/Dlg/Scribble in the regulation of epithelial junction, polarity and growth. (2008)
  • 著者名/発表者名: Yamanaka T, Ohno S
  • 雑誌名: Front Biosci 13 ページ: 6693-6707
• [雑誌論文] aPKC restricts the basolateral determinant PtdIns(3, 4, 5)P3 to the basal region. (2008)
  • 著者名/発表者名: Takahama S, Hirose T, Ohno S
  • 雑誌名: Biochem Biophys Res Commun 368(2) ページ: 249-255
• [雑誌論文] Rho-kinase phosphorylates PAR-3 and disrupts PAR complex formation. (2008)
  • 著者名/発表者名: Nakayama M, Goto T. M, Sugimoto M, Nishimura T, Shinagawa T, Ohno S, Amano M, Kaibuchi K
  • 雑誌名: Dev Cell 14(2) ページ: 205-215
• [雑誌論文] aPKC enables development of zonula adherens by antagonizing centripeta 1 contraction of the circumferential actomyosin cables (2008)
  • 著者名/発表者名: Kishikawa M, Suzuki A, Ohno S
  • 雑誌名: Journal of Cell Science 121(Pt15) ページ: 2481-2492
  • 査読あり
• [雑誌論文] The overexpression and altered localization of the atypical protein kinase C lambda/iota in breast cancer correlates with the pathologic type of these tumors (2008)
  • 著者名/発表者名: Kojima Y, Akimoto K, Nagashima Y, Ishiguro H, Shirai S, Chishima T, Ichikawa Y, Ichikawa T, Sasaki T, Kubota Y, InayamaY, Aoki I, Ohno S, Shimada H
  • 雑誌名: Hum Pathol 39(6) ページ: 824-831
  • 査読あり
• [雑誌論文] aPKC restricts the basolateral determinant PtdIns(3,4,5)P3 to the basal region (2008)
  • 著者名/発表者名: Takahama S, Hirose T, Ohno S
  • 雑誌名: Biochem. Biophys. Res. Commun 368(2) ページ: 249-255
  • 査読あり
• [雑誌論文] .aPKC restricts the basolateral determinant PtdIns(3,4,5)P3 to the basal region (2008)
  • 著者名/発表者名: Takahama S, Hirose T, Ohno S
  • 雑誌名: Biochem.Biophys.Res.Commun 368(2) ページ: 249-255
  • 査読あり
• [雑誌論文] Rho-kinase phosphorylates PAR-3 and disrupts PAR complex formation (2008)
  • 著者名/発表者名: Nakayma M, Goto TM, Sugimoto M, Nishimura T, Shinagawa T, Ohno S, Amano M, Kaibuchi K
  • 雑誌名: Dev.Cell 14(2) ページ: 205-215
  • 査読あり
• [雑誌論文] Extrinsic Wnt signalling controls the polarity component aPKC. (2007)
  • 著者名/発表者名: Ohno S
  • 雑誌名: Nat. Cell Biol. 9(7) ページ: 738-740
• [雑誌論文] Polarity-regulating kinase partitioning- defective 1/microtubule affinity-regulating kinase 2 negatively regulates development of dendrites on hippocampal neurons. (2007)
  • 著者名/発表者名: Terabayashi T, Itoh T. J, Yamaguchi H, Yoshimura Y, Funato Y, Ohno S, Miki H
  • 雑誌名: J Neurosci 27(48) ページ: 13098-107
• [雑誌論文] Loss of partitioning-defective-3/isotype -specific interacting protein (par-3/ASIP) in the elongating spermatid of RA175 (IGSF4A/SynCAM)-deficient mice. (2007)
  • 著者名/発表者名: Fujita E, Tanabe Y, Hirose T, Aurrand-Lions M, Kasahara T, Imhof B. A, Ohno S, Momoi T
  • 雑誌名: Am J Pathol 171(6) ページ: 1800-10
• [雑誌論文] Helicobacter pylori CagA targets PAR1/MARK kinase to disrupt epithelial cell polarity. (2007)
  • 著者名/発表者名: Saadat I, Higashi H, Obuse C, Umeda M, Murata-Kamiya N, Saito Y, Lu H, Ohnishi N, Azuma T, Suzuki A, Ohno S, Hatakeyama M
  • 雑誌名: Nature 447(7142) ページ: 330-333
• [雑誌論文] Distant N- and C-terminal domains are required for intrinsic kinase activity of SMG-1, a critical component of nonsense-mediated mRNA decay. (2007)
  • 著者名/発表者名: Morita T, Yamashita A, Kashima I, Ogata K, Ishiura S, Ohno S
  • 雑誌名: J. Biol. Chem. 282(11) ページ: 7799-7808
• [雑誌論文] Neonatal pancreatic cells redifferentiate into both neural and pancreatic lineages. Biochem. (2007)
  • 著者名/発表者名: Zhao W, Hirose T, Ishikawa M, Oshima Y, Hirai S, Ohno S, Taniguchi H
  • 雑誌名: Biophys. Res. Commun. 352(1) ページ: 84-90
• [雑誌論文] Polarity-regulating kinase Parlb/MARK2 negatively regulates development of dendrites on hippocampal neurons (2007)
  • 著者名/発表者名: Terabayshi T, Itoh T.J, Yamaguchi H, Yoshimura Y, Funato Y, Ohno S, Miki H
  • 雑誌名: J.Neuroscience 27(48) ページ: 13098-13107
  • 査読あり
• [雑誌論文] Loss of Partitiong-defective-3/Isotye-Specific Interacting Protein(Par-3/ASIP)in the Elongating Spermatid of RA175(IGSF4A/SynCAM)Deficieen Mice (2007)
  • 著者名/発表者名: Fujita E, Tanabe Y, Hirose T, Suzuki A, Aurrand-Lions M, Kasahara T, Imhof B.A, Ohno S, Momoi T
  • 雑誌名: AmericanJ.Pathology 171(6) ページ: 1800-1810
  • 査読あり
• [雑誌論文] Extrinsic Wnt singalling controls the polarity component aPKC (2007)
  • 著者名/発表者名: Ohno S
  • 雑誌名: Nat.Cell Biol. 9(7) ページ: 738-740
  • 査読あり
• [雑誌論文] Helicobacter pylori CagA targets PARI/MARK kinase disrupt epitheila1 ecll polarity (2007)
  • 著者名/発表者名: Saadat I, Higashi H, Obuse C, Umeda M, Murata-Kamiya N, Saito Y, Lu H, Ohnishi N, Axuma T, Suzuki A, Ohno S, Hatakeyama M
  • 雑誌名: Nature 447(7142) ページ: 330-333
  • 査読あり
• [雑誌論文] Distant N-and C-terminaldomains are required for intrins kinase activi ty Of SMG-1, a critical component of nonsense-mediated mRNA decay (2007)
  • 著者名/発表者名: Morita T, Yamashit A, Kashima I, Ogata K, Ishiura S, Ohno S
  • 雑誌名: J.Biol, Chem 282(11) ページ: 7799-7808
  • 査読あり
• [雑誌論文] Neonatal pancreatic cells redifferentiate into both neural and pancreatic li neages (2007)
  • 著者名/発表者名: Zhao W, Hirose T, Ishikawa M, Oshima Y, Hirai S, Ohno S, Taniguchi H
  • 雑誌名: Bioche, Biophys.Res.Commun 352(1) ページ: 84-90
  • 査読あり
• [雑誌論文] JNK activator DLK regulates axon growth and neuronal migration in the developing cerebral cortex. (2006)
  • 著者名/発表者名: Hirai S-I, Cui1 DF, Miyata T, Ogawa M, Kiyonari H, Suda Y, Aizawa S, Banba Y, Ohno S
  • 雑誌名: J. Neuroscience 15,26(46) ページ: 11992-12002
• [雑誌論文] Inactivation of aPKCλresults in a loss of adherens junctions in neuroepithelial cells without affecting neurogenesis in mouse neocortex. (2006)
  • 著者名/発表者名: Imai F, Hirai S-I, Akimoto K, Koyama H, Miyata T, Ogawa M, Noguchi S, Sasaoka T, Noda T, Ohno S
  • 雑誌名: Development 133(9) ページ: 1735-1744
• [雑誌論文] Lgl mediates apical domain disassembly by suppressing PAR-3/aPKC/PAR-6 complex to orient apical membrane polarity. (2006)
  • 著者名/発表者名: Yamanaka T, Horikoshi Y, Izumi N, Suzuki A, Mizuno K, Ohno S
  • 雑誌名: J. Cell Science 119 ページ: 2107-2118
• [雑誌論文] The PAR-aPKC system: lessons in polarity. (2006)
  • 著者名/発表者名: Suzuki A, Ohno S
  • 雑誌名: J Cell Sci 119 ページ: 979-987
• [雑誌論文] PAR3 is essential for cyst-mediated epicardial development by establishing apical cortical domains. (2006)
  • 著者名/発表者名: Hirose T, Karasawa M, Sugitani Y, Fujisawa M, Akimoto K, Ohno S, Noda T
  • 雑誌名: Development 133(7) ページ: 1389-1398
• [雑誌論文] Binding of a novel SMG-1-Upf1 -eRF1-eRF3 complex (SURF) to the exon junction complex triggers Upf1 phosphory- lation and nonsense-mediated mRNA decay. (2006)
  • 著者名/発表者名: Kashima I, Yamashita A, Izumi N, Kataoka N, Morishita R, Hoshino S, Ohno M, Dreyfuss G, Ohno S
  • 雑誌名: Genes Dev. 20(3) ページ: 355-367
• [雑誌論文] Inactivation of aPKC λ results in a loss of adherens junctions in neuroepithelial cells without affecting neurogenesis in mouse neocortex. (2006)
  • 著者名/発表者名: Imai F, Hirai S-I, Akimoto K, Koyama H, Miyata T, Ogawa M, Noguchi S, Sasaoka T, Noda T, Ohno S
  • 雑誌名: Development 133(9) ページ: 1735-1744
• [雑誌論文] Lgl mediates apical domain disassembly by suppressing PAR-3/aPKC/PAR-6 complex to orient apical membrane polarity. (2006)
  • 著者名/発表者名: Yamanaka T, Horikoshi Y, Izumi N, Suzuki A, Mizuno K, Ohno S
  • 雑誌名: J.Cell Science 119 ページ: 2107-2118
• [雑誌論文] The PAR-aPKC system : lessons in polarity. (2006)
  • 著者名/発表者名: Suzuki A, Ohno S
  • 雑誌名: J.Cell Science 119 ページ: 979-987
• [雑誌論文] The {gamma}-Parvin-Integrin-Linked Kinase complex is critically involved in leukocyte-substrate interaction. (2006)
  • 著者名/発表者名: Yoshimi R, Yamaji S, Suzuki A, Mishima W, Okamura M, Obana T, Matsuda C, Miwa Y, Ohno S, Ishigatsubo Y
  • 雑誌名: J.Immunology 176(6) ページ: 13611-13624
• [雑誌論文] A distinct PAR complex associates physically with VE-cadherin in vertebrate endothelial cells. (2006)
  • 著者名/発表者名: Iden S, Rehder D, August B, Suzuki A, Wolburg-Buchholz K, Woburg H, Ohno S, Behrens J, Vestweber D, Klaus E
  • 雑誌名: EMBO Rep. 7(12) ページ: 1239-1246
• [雑誌論文] Binding of a novel SMG-1-Upf1-eRF1-eRF3 complex (SURF) to the exon junction complex triggers Upflphosphorylation and nonsense-mediated mRNA decay. (2006)
  • 著者名/発表者名: Kashima I, Yamashita A, Izumi N, Kataoka N, Morishita R, Hoshino S, Ohno M, Dreyfuss G, Ohno S
  • 雑誌名: Genes Dev. 20(3) ページ: 355-367
• [雑誌論文] An association between the initial SMG-1-Upf1-eRF1-eRF3 complex and exon junction complex on mRNP is required for the SMG-1-mediated phosphorylation of Upf1 during nonsense-mediated mRNA decay. (2006)
  • 著者名/発表者名: Kashima I, Yamashita A, lzumi N, Kataoka N, Morishita R, Hoshino S, Ohno M, Dreyfuss G, Ohno S
  • 雑誌名: Genes Dev. 20(3) ページ: 355-367
• [雑誌論文] An association between the initial SMG-1-Upfl-eRF1-eRF3 complex and exon junction complex on mRNP is required for the SMG-1-mediated phosphorylation of Upf1 during nonsense-mediated mRNA decay. (2006)
  • 著者名/発表者名: Kashima I, Yamashita A, Izumi N, Kataoka N, Morishita R, Hoshino S, Ohno M, Dreyfuss G, Ohno S
  • 雑誌名: Genes Dev. 20(3) ページ: 355-367
• [雑誌論文] PAR-6-PAR-3 mediates Cdc42-induced Rac activation through the Rac GEFs STEF/Tiam1. (2005)
  • 著者名/発表者名: Nishimura T, Yamaguchi T, Kato K, Yoshizawa M, Nabeshima Y, Ohno S, Hoshino M, Kaibuchi K
  • 雑誌名: Nat. Cell Biol. 7(3) ページ: 270-277
• [雑誌論文] The role of SMG-1 on nonsense-mediated mRNA decay. (2005)
  • 著者名/発表者名: Yamashita A, Kashima I, Ohno S
  • 雑誌名: Biochem Biophys.Acta (Proteins and Proteomics), Inhibitors of Protein Kinases 1754(1-2) ページ: 305-315
• [雑誌論文] Role of SMG-1-Mediated phosphorylation of Upf1 in NMD. in Nonsense-Mediated mRNA decay. (2005)
  • 著者名/発表者名: Yamashita A, Kashima I, Ohno S
  • 雑誌名: Maquat, Landes Bioscience, (ISBN No.1-58706-296-8) - ページ: 85-95
• [雑誌論文] Function of aPKClambda in differentiating photoreceptors is required for proper lamination of mouse retina. (2005)
  • 著者名/発表者名: Koike C, Nishida A, Akimoto K, Nakaya M-A, Noda T, Ohno S, Furukawa T
  • 雑誌名: J.Neurosci. 25(44) ページ: 10290-10298
• [雑誌論文] Behavior of tight-junction, adherens-junction and cell polarity proteins during HNF-4alpha-induced epithelial polarization. (2005)
  • 著者名/発表者名: Satohisa S, Chiba H, Osanai M, Ohno S, Kojima T, Saito T, Sawada N
  • 雑誌名: Exp.Cell Res. 310(1) ページ: 66-78
• [雑誌論文] Asymmetric distribution of PAR proteins in the mouse embryo begins at the 8-cell stage during compaction. (2005)
  • 著者名/発表者名: Vinot S, Le T, Ohno S, Pawson T, Maro B, Louvet-Vallee S
  • 雑誌名: Dev.Biol. 282(2) ページ: 307-319
• [雑誌論文] Expression of MUK/DLK/ZPK, an activator of the JNK pathway, in the nervous systems of the developing mouse embryo. (2005)
  • 著者名/発表者名: Hirai S-I, Kawaguchi A, Suenaga J, Ono M, Cui de F, Ohno S
  • 雑誌名: Gene Expr.Patterns 5(4) ページ: 517-523
• [雑誌論文] PAR-6-PAR-3 mediates Cdc42-induced Rac activation through the Rac GEFs STEF/Tiam1. (2005)
  • 著者名/発表者名: Nishimura T, Yamaguchi T, Kato K, Yoshizawa M, Nabeshima Y, Ohno S, Hoshino M, Kaibuchi K
  • 雑誌名: Nat.Cell Biol. 7(3) ページ: 270-277
• [雑誌論文] Behavior of tight junction, adherens-junction and cell polarity proteins during HNF-4alpha-induced epithelial polarization. (2005)
  • 著者名/発表者名: Satohisa S, Chiba H, Osanai M, Ohno S, Kojima T, Saito T, Sawada N
  • 雑誌名: Exp.Cell Res. 310(1) ページ: 66-78
• [雑誌論文] ASPP2 Regulates Epithelial Cell Polarity through the PAR Complex.
  • 著者名/発表者名: Cong W, Hirose T, Harita Y, YamashitaA, Mizuno K, Hirano H, Ohno S.
  • 雑誌名: Current Biology (in press)
• [雑誌論文] Role of Lgl/Dlg/Scribble in the regulation of epithelial junction, polarity and growth
  • 著者名/発表者名: Yamanaka T, Ohno S
  • 雑誌名: Frontiers in Bioscience (in press)
  • 査読あり
• [雑誌論文] Symmetrically dividing cell specific division axes alteration obsered in proteasome depleted C.elegans embryo
  • 著者名/発表者名: Sugiyama Y, Nishimura AOhno S
  • 雑誌名: Mechanisms of Development (in press)
  • 査読あり
• [雑誌論文] Expression and localisation of apical junctional complex proteins in lens epithelial cells
  • 著者名/発表者名: Sugiyama Y, Prescott A.R, Tholozan^a F M D, Ohno S, Quinlan R A
  • 雑誌名: Experimental Eye Research (in press)
  • 査読あり
• [雑誌論文] The overexpression and altered localization of the atypical Protein Kinase C lambda/iota in breast cancer correlates with the pathological type of these tumors
  • 著者名/発表者名: Kojima Y, Akimoto K, Nagashima Y, Ishiguro H, Shirai S, Chishima T, Ichikawa Y, Insiakama T, Sasaki T, Knbota Y, Knbota Y, Inayama Y, Aoki I, Ohno S, Shimada H
  • 雑誌名: Human Pathology (in press)
  • 査読あり
• [学会発表] 細胞シグナリングと疾患モデルのプロテオミクス第7回大会 (2009)
  • 著者名/発表者名: 大野茂男
  • 学会等名: 日本ヒトプロテオーム機構(JHUPO)
  • 発表場所: 東京
  • 年月日: 2009-07-27
• [学会発表] The cell polarity protein, aPKClambda, plays a critical rolein the maintenance of podocyte slit diaphragms, specific type if epithelial intercellular junctions (2008)
  • 著者名/発表者名: Hirose T, Satoh D, Kurihara H, kusaka C, Hirose H, Akimoto K, Matsusaka T, Ichikawa I, Noda T, Ohno S
  • 学会等名: BMB2008
  • 発表場所: Kobe
• [学会発表] Conditionally Replicative Adenoviral Vectors for the Treatment of Gastric Cancer (2008)
  • 著者名/発表者名: Kosaka T, Ono H, Kunisaki C, Julia D, Ohno S, Yamamoto M, Shimada H
  • 学会等名: The 67th Annual Meeting of the Japanese Cancer Association
  • 発表場所: Nagoya
• [学会発表] The role of aPKC lambda in prostate cancer progression (2008)
  • 著者名/発表者名: Ishiguro H, Akimoto K, Nagashima Y, Kojima Y, Ishiguro Y, Sasaki T, Umemura H, Ohno S, Kubota Y
  • 学会等名: The 67th Annual Meeting of the Japanese Cancer Association
  • 発表場所: Nagoya
• [学会発表] Translation Termination Complex During Nonsense-Mediated MRNA Decay (2008)
  • 著者名/発表者名: Yamashita A, Izumi N, Kashima I, Katsuhata Y, Muramatsu R, Ohno S
  • 学会等名: 2008 Cold spring harver labatory meeting on translational control
  • 発表場所: New York, USA
• [学会発表] aPKC1 regulates mammary-gland morphogenesis through the transcriptiona 1 suppression of ErbB2/HER-2 gene (2008)
  • 著者名/発表者名: Akimoto K, Nagashima K, Ishiguro H, Kojima Y, Kim M, Sasaki K, Noda T, Ohno S
  • 学会等名: Second JCA-AACR Special Joint Conference, The Latest Advances in Breast Cancer Research : From Basic Science to Therapeutics
  • 発表場所: Awaji, Hyogo, Japan
• [学会発表] Possible involvement of PAR-3 in the regulation of slit diaphragm through the direct interaction with Nephrin (2008)
  • 著者名/発表者名: Satoh D, Hirose T, Kurihara H, Ohno S
  • 学会等名: The 7th International Podocyte Conference
  • 発表場所: Toronto, Canada
• [学会発表] The cell polarity protein aPKCλ plays a critical role in the maintenance of podocyte slit diaphragms (2008)
  • 著者名/発表者名: Hirose T, Satoh D, Kurihara H, Akimoto K, Matsusaka T, Ichikawa I, Noda, T, Ohno S
  • 学会等名: The 7th International Podocyte Conference
  • 発表場所: Toronto, Canada
• [学会発表] The formation of SMG-1 : Upfl : eRF1 : eRF3 complex (SURF) on ribosomes precedes translation-dependent-Upfl phosphorylation during nonsense-medi ated mRNA decay (2008)
  • 著者名/発表者名: Yamashita A, Kashima I, Izumi N, Katsuhata Y, Muramatsu R, Ohno S
  • 学会等名: THE 21st NAITO CONFERENCE"Nuclear Dynamics and RNA[I]"
  • 発表場所: Yamanashi, Japan
• [学会発表] Conditional depletion of aPKCλ in eye lens causes microphthalmia and cataract (2008)
  • 著者名/発表者名: Sugiyama Y, Akimoto K, Robinson M. L, Quinlan, R. A, Ohno S
  • 学会等名: ARVO (Association for Research in Vision and Ophthalmology) 2008Annual Meeting, 2008
  • 発表場所: Florida USA
• [学会発表] 翻訳依存的なmRNA監視複合体形成および再構成がUPfl リン酸化を誘導する (2007)
  • 著者名/発表者名: 山下 暁郎, 泉奈 津子, 鹿 勲, 森田 智子, 村松 玲子
  • 学会等名: BMB2007-第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • 発表場所: 横浜
  • 年月日: 2007-12-15
• [学会発表] 細胞極性タンパク質、PAR-1bはユーロフィン・ジストログカン複合体制御することを介して細胞外ラミニンの集積を制御している (2007)
  • 著者名/発表者名: 鈴木 厚、山下 和成、大野 茂男
  • 学会等名: BMB2007-第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • 発表場所: 横浜
  • 年月日: 2007-12-11
• [学会発表] Polarity protein PAR-1b regulates extracellular laminin organization through regulation of the utrophin-dystroglycan complex (2007)
  • 著者名/発表者名: Suzuki A, Amano Y, Yamashita K, Ohno S
  • 学会等名: Yokchama Ciyt University 21st Century COEProgram Cell Polarity2007-International Symposium, Cell Polarity System and Future Medicine
  • 発表場所: Hayama
  • 年月日: 2007-12-10
• [学会発表] Defective cell polarity in kidney podocytes causes severe glomerular disease (2007)
  • 著者名/発表者名: Hirose T, Satoh D, Kuriharawa I, Noda T, Ohno S
  • 学会等名: Yokohama City University 21st Century COE Program, Cell Polarity 2007-International symposium on CellPolarity Ststem and Future Medicine
  • 発表場所: Hayamn
  • 年月日: 2007-12-09
• [学会発表] aPKC role in prostatic cancer progression (2007)
  • 著者名/発表者名: Ishiguro H, Akimoto K, Nagashima Y, Kojima Y, Ishiguro Y, Sasaki T, Uemura H, Ohono S, Kubota Y
  • 学会等名: Yokohama City University 21st Century COE Program, Cell Polarity 2007-International symposium on CellPolarity Ststem and Future Medicine
  • 発表場所: Hayama
  • 年月日: 2007-12-09
• [学会発表] The role of apkcl in breast cancer (2007)
  • 著者名/発表者名: Akimoto K
  • 学会等名: Yokohama City University 21st Century COE Program, Cell Polarity 2007-International symposium on CellPolarity Ststem and Future Medicine
  • 発表場所: Hayama
  • 年月日: 2007-12-09
• [学会発表] The role of PAR1/MARK polarity kinase in the interaction between Heli civacter pylori CagA and SHP2 oncoprotein (2007)
  • 著者名/発表者名: 紙谷 尚子, Iraj Saadat, 東 秀明, 小布施 力史, 梅田 真由美, 齊藤 康弘, Huaischeng Lu, 大西 なおみ, 東 健, 鈴木 厚, 大野 茂男, 畠山 昌則
  • 学会等名: 第66回日本癌学会学術総会
  • 発表場所: 横浜
  • 年月日: 2007-10-03
• [学会発表] The role of the PAR-aPKC complex in epithelial morphogenesis and tum origenesis (2007)
  • 著者名/発表者名: 大野 茂男
  • 学会等名: 第66回日本癌学会学術総会
  • 発表場所: 横浜
  • 年月日: 2007-10-03
• [学会発表] The SMG-1:SMG-8:p60 Complex Regulated Phosphorylation Of Upf1Is Repuired For The Dissociation Of eRFs From mRNA Surveillance Complex During Nonsense-mediated mRNA decay (2007)
  • 著者名/発表者名: Yamashita A, Izumi N, Kashima I, Ohnishi T, Amderson P, Ohno S.
  • 学会等名: 第9回日本RNA学会年会
  • 発表場所: 名古屋
  • 年月日: 2007-07-28
• [学会発表] The SMG-1:SMG-8:p60 Complex Regulated Phosphorylation OF Upf1 Is Required For The Dissociation Of eRFs From mRNA Surveillance Complex During Nonsense-mediated mRNA decay (2007)
  • 著者名/発表者名: Yamashita A, Kashima I, Izumi N, Ohnishi T, Anderson P, Ohno S.
  • 学会等名: 第12回国際RNA学会年会
  • 発表場所: University of Wisconsin,Madison
  • 年月日: 2007-06-03
• [学会発表] A novel signaling pathway by which PAR-aPKC system regulates cell-substate interaction (2007)
  • 著者名/発表者名: 鈴木 厚、山下 和成、天野 芳子、大野 茂男
  • 学会等名: 第40回日本発生生物学会, 第59回細胞生物学会合同大会シンポジウム
  • 発表場所: 福岡
  • 年月日: 2007-05-30
• [学会発表] mRNAの品質鹽機構〜分子機構とその振作技術の物学・医学への展開〜 (2007)
  • 著者名/発表者名: 大野 茂男
  • 学会等名: 日本生化学北陸支部会
  • 発表場所: 金沢
  • 年月日: 2007-05-26
• [図書] 生化学・第2版 (2007)
  • 著者名/発表者名: 大野 茂男
  • 総ページ数: 217
  • 出版者: 東京化学同人
• [備考] 新聞報道
• [備考] 2010.4.13 経産業新聞、DNA損傷防ぐ仕組み解明
• [備考] 2009.10.20 日経産業新聞、前立腺がん再発関連の遺伝子発見
• [備考] 2009.2.22 読売新聞、腎糸球体の血液ろ過膜機能維持に新たな機構
• [備考] ホームページ
  • URL: http://www-user.yokohama-cu.ac.jp/~ohnos//Japanease/indexJ.html
• [備考]
  • URL: http://www-user.yokohama-cu.ac.jp/~ohnos/Japanease/indexJ.html
• [備考]
  • URL: http://www-user.yokohama-cu.acjp/?ohnos//Japanease/indexihtnal
• [備考]
  • URL: http://www-user.yokohama-cu.ac.jp/~ohnos/Japanease/indexJ.html
• [産業財産権] 初期乳がんモデル用動物 (2009)
  • 発明者名: 秋本和憲、大野茂男
  • 権利者名: 公立大学法人横浜市立大学
  • 出願年月日: 2009-06-18
• [産業財産権] 初期乳がんモデル用動物 (2007)
  • 発明者名: 秋本和憲、野田哲生、大野茂男
  • 権利者名: 横浜市立大学
  • 産業財産権番号: 2007-167036
  • 出願年月日: 2007-06-25
• [産業財産権] mRNAサーベイランス経路を制御するタンパク質 (2007)
  • 発明者名: 山下暁朗、大野茂男
  • 権利者名: 横浜市立大学
  • 産業財産権番号: 2007-179140
  • 出願年月日: 2007-07-08