| Project/Area Number |
17591729
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
HIGUCHI Toshihiro KYOTO UNIVERSITY, Graduate school of Medicine, Lecturer, 医学研究科, 講師 (00283614)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUHARA Ken KYOTO UNIVERSITY, Graduate school of Medicine, Assistant Professor, 医学研究科, 助手 (00362533)
TAKAKURA Kenji KYOTO UNIVERSITY, Graduate school of Medicine, Associate Professor, 医学研究科, 助教授 (10221350)
FUJII Shingo KYOTO UNIVERSITY, Graduate school of Medicine, Professor, 医学研究科, 教授 (30135579)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | implantation / stress / infertility / mouse |
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| Research Abstract |
Background : It has been proposed that ovarian endocrine factors play an important role for the successful implantation. However, some patients fail to pregnant even though their ovarian function appears normal and enough to induce endometrial differentiation to prepare pregnancy, decidualization. Such patients, implantation failure, are difficult to treat in spite of the recent development of ART. It is well known that chronic stress induces various reproductive disorder including aovulation and abortion. However, the influence of maternal stress on embryo implantation is not well clarified. Therefore, we analyzed whether maternal stress affect the mouse embryo implantation.
Results : At first, we examined the influence of stress on the reproductive function of normal pregnant or pseudopregnant and embryo-transferred mouse. Maternal stress induced pregnancy loss in both groups. However, these findings were the results of stress induced ovarian dysfunction rather than the implantation f
… More
ailure itself. We nextly examined the influence of stress on delayed implantation model. This model replaces ovarian function exogenously after ovariectomy, so it appeared possible to investigate the influence of stress on embryo implantation without affecting ovarian function. However, surgical stress of ovariectomy appeared to obscure the influence of subsequent chronic stress.
Therefore, we prepared ovariectomized female mice and transferred murine blastocysts after supplementation of exogenous ovarian steroids. By the pretreatment with estradiol in addition to estradiol/ progesterone administration during peri-implantation period, we succeeded to induce satisfactory implantation rate compared with conventional pseudopregnant and embryo transfer model Using this mouse model, we examined the influence of maternal stress on the implantation of mouse embryo. Continuous ultrasonic stress from 3days before the embryo transfer significantly suppressed implantation rate compared with control group. Presently, we are investigating the gene profile of this stress induced mouse implantation failure using cDNA microarray analysis.
Conclution : We could demonstrated that stress itself induces implantation failure in mouse. Further investigation might clarify the mechanism of this stress induced implantation failure, which would lead to the development of a novel treatment strategy of unexplained implantation failure. Less
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