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Studies on the mechanisms of cell toxicity caused by hydrogen sulfide

Research Project

Project/Area Number 21590733
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionTokyo Medical and Dental University

Principal Investigator

UEMURA Kouichi  東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (30244586)

Co-Investigator(Kenkyū-buntansha) FUNAKOSHI Takeshi  東京医科歯科大学, 大学院・医歯学総合研究科, 助教 (40444715)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2011: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywords硫化水素 / 心筋由来細胞 / 肺胞上皮由来細胞 / アポトーシス / 肺胞上皮 / 心筋細胞 / 細胞死 / 心筋
Research Abstract

To elucidate the mechanism of toxic effects of H2S on cells by exposure to NaHS as a donor of H2S in cells derived from fetal rat cardiomyocytes(H9c2 cells) or rat type II alveolar epithelial cells(L2 cells) was performed. In H9c2 cells atrophy of the cytoplasm was observed under 3mM NaHS after a dozen hours. Remarkable morphological apoptotic cell death and the activation of caspase3 were observed in 5mM NaHS. In L2 cells after an hour under 2-3mM NaHS exposure, nuclear aggregation, AnnecinV positivity with cell membrane, leakage into the cytoplasm of Cytochrome C has been confirmed, which showed that the concentration of NaHS cytotoxicity in L2 cells is lower than in H9c2 cells. H2S cytotoxic sensitivity was different by organs.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (6 results)

All 2010 Other

All Presentation (4 results) Remarks (2 results)

  • [Presentation] 心筋細胞における硫化水素の毒性の検討2010

    • Author(s)
      白鳥典, 船越丈司, 鵜沼香奈, 秋利彦, 上村公一
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 硫化水素による心筋細胞障害の検討2010

    • Author(s)
      白鳥典、船越丈司、鵜沼香奈、秋利彦、上村公一
    • Organizer
      第79回日本法医学会学術関東地方集会
    • Place of Presentation
      東京
    • Year and Date
      2010-10-30
    • Related Report
      2011 Final Research Report
  • [Presentation] 硫化水素による心筋細胞障害の検討2010

    • Author(s)
      白鳥典, 船越丈司, 鵜沼香奈, 秋利彦, 上村公一
    • Organizer
      第79回日本法医学会学術関東地方集会
    • Place of Presentation
      東邦大(東京都)
    • Year and Date
      2010-10-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 心筋細胞における硫化水素の毒性の検討2010

    • Author(s)
      白鳥典、船越丈司、鵜沼香奈、秋利彦、上村公一
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.tmd.ac.jp/med/legm/kenkyu.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.tmd.ac.jp/med/legm/kenkyu.html

    • Related Report
      2011 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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