From in vitro cultivation to rapid diagnosis of hemotropic mycoplasmas

• Project/Area Number: 23658251
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied veterinary science
• Research Institution: Iwate University
• Principal Investigator: HARASAWA Ryo 岩手大学, 農学部, 教授 (70159101)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ヘモプラズマ / マイコプラズマ / 動物衛生 / 獣医微生物学 / Mycoplasma

Research Abstract

Hemoplasma is a prokaryote that parasites on mammalian erythrocytes and causes a hemolytic anemia in the infected animals. However, its prevalence in the Japanese cattle has remained unknown. Therefore, hemoplasma infection in cattle was examined in this study. Here we demonstrated a wide prevalence of hemoplasma infection among cattle population in Japan.

Research Products

• [Journal Article] Hemotropic mycoplasma infection in wild black bear (Ursus thibetanus japonicus) (2013)
  • Author(s): Iso, T.et al.
  • Journal Title: Veterinary Microbiology Volume: 163 Pages: 184-189
  • Peer Reviewed
• [Journal Article] Two genotypes among 'Candidatus Mycoplasma haemobos' strains based on tne 16S-23S rRNA intergenic spacer sequences. (2013)
  • Author(s): Sasaoka, F. et al.
  • Journal Title: The Journal of Veterinary Medical Science Volume: 75 Pages: 361-365
  • Peer Reviewed
• [Journal Article] The bacterial cell division protein FtsZ from rings in swarmer cells of Proteus mirabilis. (2013)
  • Author(s): Fujihara, M. et al.
  • Journal Title: Annals of Microbiology Volume: 63 Pages: 399-401
  • Peer Reviewed
• [Journal Article] Examination of the 16S-23S rRNA intergenic spacer sequences of 'Candidatus Mycoplasma haemobos' and Mycoplasma haemofelis (2012)
  • Author(s): Sasaoka, F., Suzuki, J., Fujihara, M., Watanabe, Y., Nagai, K., and Harasawa, R.
  • Journal Title: J. Vet. Med. Sci Volume: 74 Pages: 83-87
  • Peer Reviewed
• [Journal Article] Prevalence of swine hemoplasmas revealed by real-time PCR using 16S rRNA gene primers (2012)
  • Author(s): Watanabe, Y., Fujihara, M., Suzuki, J., Sasaoka, F., Nagai, K., and Harasawa, R.
  • Journal Title: J. Vet. Med. Sci Volume: 74 Pages: 1315-1318
  • Peer Reviewed
• [Journal Article] Seroepidemiological survey of sheep flocks from northern Japan for Mycoplasma ovipneumoniae and Mycoplasma agalactiae (2012)
  • Author(s): Giangaspero, M., Nicholas, R.A., Hlusek, M., Bonfini, B., Osawa, T., Orusa, R., Tatami, S., Takagi, E., Moriya, H., Okura, N., Kato, K., Kimura, A., Harasawa, R., and Ayling, R.D.
  • Journal Title: Trop. Anim. Health Prod Volume: 44 Pages: 395-398
  • Peer Reviewed
• [Journal Article] Serological screening of Coxiella burunetti (Q fever) and Brucella spp. in sheep flocks in the northern prefectures of Japan in 2007 (2012)
  • Author(s): Giangaspero, M., Osawa, T., Bonfini, B., Orusa, R., Robetto, S., and Harasawa, R.
  • Journal Title: Vet. Ital Volume: 48 Pages: 357-365
  • Peer Reviewed
• [Journal Article] Examination of the 16S-23S rRNA Intergenic Spacer Sequences of Candidatus Mycoplasma haemobos' and Mycoplasma haemofelis (2012)
  • Author(s): Sasaoka, F., Suzuki, J., Fujihara, M., Watanabe, Y., Nagai, K., Harasawa, R.
  • Journal Title: Journal of Veterinary Medical Science Volume: 74 Issue: 1 Pages: 83-87
  • DOI: 10.1292/jvms.11-0344
  • NAID: 130001032840
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Mycoplasma ovis detected in free-living Japanese serows, Capricirnis crispus (2011)
  • Author(s): Ohtake, Y., Nishizawa, I., Sato, M., Watanabe, Y., Nishimura, T., Matsubara, K., Nagai, K., and Harasawa, R.
  • Journal Title: J. Vet. Med. Sci Volume: 73 Pages: 371-373
  • Peer Reviewed
• [Journal Article] A feline hemoplasma, 'Candidatus Mycoplasma haematominum', detected in dog in Japan (2011)
  • Author(s): Obara, H., Fujihara, M., Watanabe, Y., Ono, H.K., and Harasawa, R.
  • Journal Title: J. Vet. Med. Sci Volume: 73 Pages: 841-843
  • Peer Reviewed
• [Journal Article] Species characterization in the genus Pestivirus according to palindromic nucleotide substitutions in the 5'-untranslated region (2011)
  • Author(s): Giangaspero, M., and Harasawa, R.
  • Journal Title: J. Virol. Methods Volume: 174 Pages: 166-172
  • Peer Reviewed
• [Journal Article] Molecular identification of 'Candidatus Mycoplasma haemovis' in sheep with hemolytic anemia (2011)
  • Author(s): Suzuki, J., Sasaoka, F., Fujihara, M., Watanabe, Y., Tasaki, T., Oda, S., Kobayashi, S., Sato, R., Nagai, K., and Harasawa, R.
  • Journal Title: J. Vet. Med. Sci Volume: 73 Pages: 1113-1115
  • Peer Reviewed
• [Journal Article] Acidic environments induce differentiation of Proteus mirabilis into swarmer morphotypes. Microbiol (2011)
  • Author(s): Fujihara, M., Obara, H., Watanabe, Y., Ono, H.K., Sasaki, J., Goryo, M., and Harasawa, R.
  • Journal Title: Immunol Volume: 55 Pages: 489-493
  • Peer Reviewed
• [Journal Article] Rapid identification of hemoplasma species by palindromic nucleotide substitutions at the GAAA tetraloop helix in the specificity domain of ribonuclease P RNA (2011)
  • Author(s): Sasaoka, F., Suzuki, J., Watanabe, Y., Fujihara, M., and Harasawa, R.
  • Journal Title: J. Vet. Med. Sci Volume: 73 Pages: 1517-1520
  • Peer Reviewed
• [Journal Article] Epidemiological survey of Border disease virus among sheep from northern districts of Japan (2011)
  • Author(s): Giangaspero, M., Ibata, G., Savini, G., Osawa, T., Tatami, S., Takagi, E., Moriya, H., Okura, N., Kimura, A., and Harasawa, R.
  • Journal Title: J. Vet. Med. Sci Volume: 73 Pages: 1629-1633
  • Peer Reviewed
• [Journal Article] Two genetic clusters in swine hemoplasmas revealed by analyses of the 16S rRNA and RNase P RNA genes (2011)
  • Author(s): Watanabe, Y., Fujihara, M., Obara, H., Nagai, K., and Harasawa, R.
  • Journal Title: J. Vet. Med. Sci Volume: 73 Pages: 1657-1661
  • Peer Reviewed
• [Journal Article] Epidemiological survey for visna-maedi among sheep in northern prefectures of Japan (2011)
  • Author(s): Giangaspero, M., Osawa, T., Orusa, R., Frossard, J.-P., Naidu, B., Robetto, S., Tatami, S., Takagi, E., Moriya, H., Okura, N., Kato, K., Kimura, A., and Harasawa, R.
  • Journal Title: Vet. Ital Volume: 47 Pages: 437-451
  • Peer Reviewed
• [Journal Article] Prevalence of hemoplasma infection among cattle in the western part of Japan (2011)
  • Author(s): Fujihara, Y., Sasaoka, F., Suzuki, J., Watanabe, Y., Fujihara, M., Ooshita, K., Ano, H., and Harasawa, R.
  • Journal Title: J. Vet. Med. Sci Volume: 73 Pages: 1653-1655
  • Peer Reviewed
• [Journal Article] Prevalence of hemoplasma infection among cattle in the western part of Japan (2011)
  • Author(s): Fujihara, Y., Sasaoka, F., Suzuki, J., Watanabe, Y., Fujihara, M., Ooshita, K., Ano, H., Harasawa, R.
  • Journal Title: Journal of Veterinary Medical Science Volume: 73 Pages: 1653-1655
  • Peer Reviewed
• [Journal Article] Rapid identification of hemoplasma species by palindromic nucleotide substitution at the GAAAtetraloop helix in the specificity domain of ribonuclease P RNA (2011)
  • Author(s): Sasaoka, F., Suzuki, J., Watanabe, Y., Fujihara, M., Harasawa, R.
  • Journal Title: Journal of Veterinary Medical Science Volume: 73 Pages: 1517-1520
  • Peer Reviewed