絶滅危惧両生類における遺伝的多様性評価と保全のための包括的研究

• Project/Area Number: 24310173
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Resource conservation science
• Research Institution: Hiroshima University
• Principal Investigator: 住田 正幸 広島大学, 理学(系)研究科(研究院), 教授 (10163057)
• Co-Investigator(Kenkyū-buntansha): 倉林 敦 広島大学, 理学(系)研究科(研究院), 助教 (00327701) ; 井川 武 広島大学, 国際協力研究科, 助教 (00507197) ; ISLAM Md.Mafizul (ISLAM M.Mafizul) 広島大学, 理学(系)研究科(研究院), 特任助教 (40598976)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Discontinued (Fiscal Year 2015)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
• Keywords: 両生類 / 絶滅危惧種 / 遺伝的多様性 / 保全 / 飼育下繁殖 / 人工繁殖 / マイクロサテライト / 精子凍結保存 / MHC

Outline of Annual Research Achievements

① マイクロサテライト（SSR）の開発とこれらを用いた集団構造の解析：南西諸島産絶滅危惧種イシカワガエル・アマミイシカワガエル・イボイモリ・オットンガエル・ホルストガエルおよびバングラデシュ産トラフガエルについてSSRマーカーを開発し、これらを用いてジェノタイピングを行い、各種について集団内の遺伝的多様性と集団間の遺伝的構造を解明した。
② 種ごとに適切な人工受精法と飼育法の確立：南西諸島産絶滅危惧種イシカワガエル・アマミイシカワガエル・イボイモリ・ホルストガエル・オットンガエル・アマミハナサキガエルについて、各種各集団について、人工受精と飼育条件の最適化を行い、各種に適切な飼育法を確立した。アマミイシカワガエルとイボイモリについては飼育下での自然繁殖によって２代目が誕生した。
③精子凍結保存法の確立の試み：南西諸島産の準絶滅危惧種アマミアカガエルと絶滅危惧種アマミイシカワガエルを用いて、超低温槽を用いた精子凍結保存法の確立を試みた。24時間～1年間凍結保存後の精子を解凍し、精子の運動を観察するとともに、人工受精法によって受精率を測定した。その結果、試みたどの期間でも解凍精子にある程度の運動能がみられ、24時間凍結精子の受精率は、アマミアカガエルでは最大86%、アマミイシカワガエルでは最大35％であった。
④遺伝マーカーによる血縁度測定と種親選定による計画的交配：アマミイシカワガエルの飼育下繁殖個体を用いて、血縁度の異なるいろいろな組合せで人工交配を行い、それぞれの子孫について生活力を検証し、飼育下で遺伝的多様性を維持できる継代飼育システムを確立した。

Research Progress Status

26年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

26年度が最終年度であるため、記入しない。

Expenditure Plan for Carryover Budget

26年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Development and Validation of PCR-RFLP Assay to Identify Three Japanese Brown Frogs of the True Frog Genus <i>Rana</i> (2015)
  • Author(s): Igawa T., S. Komaki, T. Takahara, and M. Sumida
  • Journal Title: Current Herpetology Volume: 34 Issue: 1 Pages: 89-94
  • DOI: 10.5358/hsj.34.89
  • NAID: 130004888109
  • ISSN: 1345-5834, 1881-1019
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genetic population structure and demographic history of an endangered frog, Babina holsti (2015)
  • Author(s): Kakehashi R., T. Igawa, and M. Sumida
  • Journal Title: Conservation Genetics Volume: 16 Issue: 4 Pages: 987-1000
  • DOI: 10.1007/s10592-015-0718-0
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Development and characterization of 27 new microsatellite markers for the Indian bullfrog <i>Hoplobatrachus tigerinus</i> and its congeneric species (2014)
  • Author(s): Nasrin S., T. Igawa, M. Nozawa, M. M. Islam, M. Hasan, M. S. Alam, M. M. R. Khan, and M. Sumida
  • Journal Title: Genes & Genetic Systems Volume: 89 Issue: 3 Pages: 137-141
  • DOI: 10.1266/ggs.89.137
  • NAID: 130004703732
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Microsatellite Marker development by Multiplex Ion Torrent PGM Sequencing: A case study of the endangered Odorrana narina complex of frogs (2014)
  • Author(s): Igawa T., M. Nozawa, M. Nagaoka, S. Komaki, S Oumi, T Fujii, and M Sumida
  • Journal Title: Journal of Heredity Volume: 106 Issue: 1 Pages: 131-137
  • DOI: 10.1093/jhered/esu071
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genetic divergence and geographic distribution of frogs in genus Fejervarya from Indonesia from mitochondrial 16S rRNA gene analysis (2014)
  • Author(s): Kurniawan, N., T. H. Djong, T. Maideliza, A. Hamidy, M. Hasan, T. Igawa and M. Sumida
  • Journal Title: Treubia Volume: 41 Pages: 1-16
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 両生類「ノアの方舟」プロジェクト-両生類絶滅危惧種の飼育下繁殖による保全の試み- (2014)
  • Author(s): 住田正幸
  • Journal Title: 生物工学会誌 Volume: 92 Pages: 375-377
  • NAID: 110009843941
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Genetic divergences of South and Southeast Asian frogs : a case study of several taxa based on 16S ribosomal RNA gene data with notes on the generic name Fejervarya (2014)
  • Author(s): Mahmudul Hasan, Mohammed Mafizul Islam, Mukhlesur Rahman Khan, and Takeshi Igawa, Mohammad Shafiqul Alam, HonTjong Djong, Nia Kurniawan, Hareesh Joshy, Yong Hoi Sen, Daicus M. Belabut, Atsushi Kurabayashi, Mitsuru Kuramoto, Masayuki Sumida
  • Journal Title: Turkish Journal of Zoology Volume: 38 Pages: 1-22
  • DOI: 10.3906/zoo-1308-36
  • Peer Reviewed / Open Access
• [Journal Article] Improved Transport of the Model Amphibian, <i>Xenopus tropicalis</i>, and Its Viable Temperature for Transport (2014)
  • Author(s): Kurabayashi, A., R. Kakehashi, I. Tazawa, Y. Haramoto, T. Oshima, Y. Ito and M. Sumida
  • Journal Title: Current Herpetology Volume: 33 Issue: 1 Pages: 75-87
  • DOI: 10.5358/hsj.33.75
  • NAID: 130003393156
  • ISSN: 1345-5834, 1881-1019
  • Peer Reviewed
• [Journal Article] Development and characterization of 14 microsatellite markers for Buergeria japonica (Amphibia, Anura, Rhacophoridae) (2014)
  • Author(s): Komaki, S., T. Igawa, M. Nozawa, S.-M. Lin, S. Oumi and M. Sumida
  • Journal Title: Genes Genet. Syst. Volume: 89 Pages: 35-39
  • NAID: 130004137821
  • Peer Reviewed
• [Journal Article] Mitochondrial genomes of Japanese Babina frogs (Ranidae, Anura): unique gene arrangements and the phylogenetic position of genus Babina (2013)
  • Author(s): Kakehashi, R., A. Kurabayashi, S. Oumi, S. Katsuren, M. Hoso and M. Sumida
  • Journal Title: Genes Genet. Syst. Volume: 88 Pages: 59-67
  • NAID: 10031183171
  • Peer Reviewed
• [Journal Article] Development and characterization of new microsatellite loci in the Otton frog (Babina subaspera) and cross-amplification in a congeneric species, Holst’s frog (B. holsti). (2013)
  • Author(s): Ryosuke Kakehashi, Takeshi Igawa, Noriko Iwai, Etsuko Shoda-Kagaya, and Masayuki Sumida
  • Journal Title: Conservation Genetics Resources Volume: 5 Issue: 4 Pages: 1071-1073
  • DOI: 10.1007/s12686-013-9981-x
  • Peer Reviewed
• [Journal Article] Attempt at Captive Breeding of the Endangered Newt Echinotriton andersoni, from the Central Ryukyus in Japan. (2013)
  • Author(s): Takeshi Igawa, Hirotaka Sugawara, Miyuki Tado, Takuma Nishitani, Atsushi Kurabayashi, Mohammed Islam, Shohei Oumi, Seiki Katsuren, Tamotsu Fujii, Masayuki Sumida
  • Journal Title: Animals Volume: 3 Issue: 3 Pages: 680-692
  • DOI: 10.3390/ani3030680
  • NAID: 120006523852
  • Peer Reviewed
• [Journal Article] Description of two new species of Microhyla (Anura: Microhylidae) from Bangladesh (2013)
  • Author(s): Hasan, M., M. M. Islam, M. Kuramoto, A. Kurabayashi and M. Sumida
  • Journal Title: Zootaxa Volume: 3755 Issue: 5 Pages: 401-418
  • DOI: 10.11646/zootaxa.3755.5.1
  • Peer Reviewed
• [Journal Article] Afrobatrachian mitochondrial genomes : genome reorganization, gene rearrangement mechanisms, and evolutionary trends of duplicated and rearranged genes (2013)
  • Author(s): Kurabayashi, A. & M. Sumida
  • Journal Title: BMC Genomics Volume: 14 Issue: 1 Pages: 633-633
  • DOI: 10.1186/1471-2164-14-633
  • Peer Reviewed
• [Journal Article] Population structure and landscape genetics of two endangered frog species of genus Odorrana: different scenarios on two islands (2013)
  • Author(s): Takeshi Igawa, Shohei Oumi, Seiki Katsuren and Masayuki Sumida
  • Journal Title: Heredity Volume: 110 Issue: 1 Pages: 46-56
  • DOI: 10.1038/hdy.2012.59
  • Peer Reviewed
• [Journal Article] Mitochondrial genomes of Japanese Babina frogs (Ranidae, Anura) : unique gene arrangements and the phylogenetic position of genus Babina (2013)
  • Author(s): Kakehashi, R., A. Kurabayashi, S. Oumi, S. Katsuren, M. Hoso and M. Sumida
  • Journal Title: Genes & Genetic Systems Volume: 88(印刷中)
  • NAID: 10031183171
  • Peer Reviewed
• [Journal Article] Cryptic anuranbiodiversity in Bangladesh revealed by mitochondrial 16S rRNA gene sequences (2012)
  • Author(s): Hasan, M., M. M. Islam, M. M. R. Khan, M. S. Alam, A. Kurabayashi, T. Igawa, M. Kuramoto, and M. Sumida
  • Journal Title: Zoological Science Volume: 29 Pages: 162-172
  • NAID: 120006473862
  • Peer Reviewed
• [Journal Article] Isolation and characterization of ten microsatellite loci of endangered Anderson’s crocodile newt, Echinotriton andersoni. (2012)
  • Author(s): Sugawara Hirotaka, Takeshi Igawa, Masashi Yokogawa. Masaru Okuda, Shohei Oumi, Seiki Katsuren, Singo Kaneko, Tetsuya Umino, Yuji Isagi and Masayuki Sumida
  • Journal Title: Conservation Genetics Resources Volume: 7 Issue: 3 Pages: 595-598
  • DOI: 10.1007/s12686-012-9600-2
  • Peer Reviewed
• [Journal Article] Distributional change and epidemic introgression in overlapping areas of Japanese pond frog species over 30 years (2012)
  • Author(s): Komaki, S., A. Kurabayashi, M. M. Islam, K. Tojo, and M. Sumida
  • Journal Title: Zoological Science Volume: 29 Issue: 6 Pages: 351-358
  • DOI: 10.2108/zsj.29.351
  • NAID: 120006473863
  • Peer Reviewed
• [Journal Article] Mitochondrial genomes and divergence times of crocodile newts: Inter-islands distribution of <i>Echinotriton andersoni</i> and the origin of a unique repetitive sequence found in <i>Tylototriton</i> mt genomes (2012)
  • Author(s): Kurabayashi, A., T. Nishitani, S. Katsuren, S. Oumi, and M. Sumida
  • Journal Title: Genes & Genetic Systems Volume: 87 Issue: 1 Pages: 39-51
  • DOI: 10.1266/ggs.87.39
  • NAID: 10030616227
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed
• [Journal Article] A new species of genus Hoplobatrachus (Anura, Dicroglossidae) from the coastal belt of Bangladesh (2012)
  • Author(s): Hasan, M., M. Kuramoto, M. M. Islam, M. S. Alam, M. M. R. Khan and M. Sumida
  • Journal Title: Zootaxa Volume: 3312 Pages: 45-58
  • Peer Reviewed
• [Journal Article] Postmating isolation in six species of three genera (Hoplobatrachus, Euphlyctis and Fejervarya)from family Dicroglossidae (Anura), with special reference to spontaneous production of allotriploids (2012)
  • Author(s): Alam, M. S., M. M. Islam, M. M. R. Khan, M. Hasan, R. Wanichanon and M. Sumida
  • Journal Title: Zoological Science Volume: 29 Issue: 11 Pages: 743-752
  • DOI: 10.2108/zsj.29.743
  • NAID: 40019472073
  • Peer Reviewed
• [Presentation] Cryopreservation of sperm using endangered and near-threatened frogs from the Ryukyu Archipelago: an ex situ conservation effort (2014)
  • Author(s): Mohammed Mafizul Islam, Ryosuke Kakehashi, Oumi Sohei, Katsuren, Atsushi Kurabayashi, Masayuki Sumida
  • Organizer: 日本爬虫両棲類学会第53回大会
  • Place of Presentation: 神戸山手大学、神戸
  • Year and Date: 2014-11-09
• [Presentation] ヘビからカエルへの遺伝子水平伝播：発見の経緯と発生頻度の地理的相違（ポスター発表） (2014)
  • Author(s): 倉林 敦・住田正幸・大島一彦・Miguel Vences
  • Organizer: 日本爬虫両棲類学会第53回大会
  • Place of Presentation: 神戸山手大学、神戸
  • Year and Date: 2014-11-08 – 2014-11-09
• [Presentation] 東アジア産トノサマガエル種群の遺伝子浸透 (2014)
  • Author(s): 小巻翔平・井川 武・林 思民・東城幸治・Min-Mi-Sook・住田正幸
  • Organizer: 日本爬虫両棲類学会第53回大会
  • Place of Presentation: 神戸山手大学、神戸
  • Year and Date: 2014-11-08
• [Presentation] Morphological and molecular comparisons of two-striped grass frogs(Anura: Ranidae) from Bangladesh and Taiwan (2014)
  • Author(s): Mahmudul Hasan, June-Shiang Lai, Masayuki Sumida
  • Organizer: 日本爬虫両棲類学会第53回大会
  • Place of Presentation: 神戸山手大学、神戸
  • Year and Date: 2014-11-08
• [Presentation] Fine scale genetic divergence among Hoplobatrachus tigernus from Bangladesh and neighboring countries elucidated by mitochondrial genes and microsatellite markers (2014)
  • Author(s): Nasrin Sultana, Takeshi Igawa, Masafumi Nozawa, Mohammed Mafizul Islam, Mahmudul Hasan, Mohammad Shafiqul Alam, Md. Mukhlesur Rahman Khan, Masayuki Sumida
  • Organizer: 日本爬虫両棲類学会第53回大会
  • Place of Presentation: 神戸山手大学、神戸
  • Year and Date: 2014-11-08
• [Presentation] 次世代モデルリソース・ネッタイツメガエル7系統における近交度及び遺伝的関係 (2014)
  • Author(s): 渡辺 愛・井川 武・柏木昭彦・鈴木 厚・倉林 敦・藤井 保・住田正幸
  • Organizer: 日本爬虫両棲類学会第53回大会
  • Place of Presentation: 神戸山手大学、神戸
  • Year and Date: 2014-11-08
• [Presentation] Evolutionary relationships and postmating isolation among Fejervarya species from Lesser Sunda, Indonesia and other Asian countries revealed by mtDNA Cyt b gene sequences and crossing experiments (2014)
  • Author(s): Hasan, M., N. Kurniawan, A. Soewondo, M. M. Islam, T. Igawa, W. M. M. Nalley, M. Matsui and M. Sumida
  • Organizer: International Symposium “Frontiers in Amphibian Biology”
  • Place of Presentation: Hiroshima University, Higashihiroshima
• [Presentation] Artificial Breeding of an Indian Bullfrog (Hoplobatrachus tigerinus): A Case Study on Reproduction and Conservation (2014)
  • Author(s): Alam, M. S., M. M. R. Khan, M. M. Islam, M. Hasan and M. Sumida
  • Organizer: International Symposium “Frontiers in Amphibian Biology”
  • Place of Presentation: Hiroshima University, Higashihiroshima
• [Presentation] Translucent frogs created through crossbreeding, and their inheritance and dermal chromatophore structure (2014)
  • Author(s): Sumida, M., M. M. Islam, T. Igawa, A. Kurabayashi, Y. Furukawa, N. Sano, T. Fujii and N. Yoshizaki
  • Organizer: International Symposium “Frontiers in Amphibian Biology”
  • Place of Presentation: Hiroshima University, Higashihiroshima
• [Presentation] Inter- and intra-island divergence in Odorrana ishikawae (Anura, Ranidae) of the Ryukyu Archipelago of Japan, with description of a new species (2014)
  • Author(s): Kuramoto, M., N. Satou, S. Oumi, A. Kurabayashi, and M. Sumida
  • Organizer: International Symposium “Frontiers in Amphibian Biology”
  • Place of Presentation: Hiroshima University, Higashihiroshima
• [Presentation] A new species of the Fejervarya limnocharis complex from Japan (Anura, Dicroglossidae) (2014)
  • Author(s): Djong, H. T., M. Matsui, M. Kuramoto, M. Nishioka, and M. Sumida
  • Organizer: International Symposium “Frontiers in Amphibian Biology”
  • Place of Presentation: Hiroshima University, Higashihiroshima
• [Presentation] Development and characterization of 27 new microsatellite markers of Indian Bullfrog, Hoplobatrachus tigerinus (Daudin, 1802) and its congeneric species (2014)
  • Author(s): Sultana, N., T. Igawa, M. Nozawa, M. M. Islam, M. M. R. Khan, M. Hasan, M. S. Alam and M. Sumida
  • Organizer: International Symposium “Frontiers in Amphibian Biology”
  • Place of Presentation: Hiroshima University, Higashihiroshima
• [Presentation] Genetic population structure of an endangered frog species, Babina holsti (2014)
  • Author(s): Kakehashi, R., T. Igawa and M. Sumida
  • Organizer: International Symposium “Frontiers in Amphibian Biology”
  • Place of Presentation: Hiroshima University, Higashihiroshima
• [Presentation] New insights into evolutionary history among Asian pond frog species (2014)
  • Author(s): Komaki, S., T. Igawa, K. Tojo, A. Kurabayashi, S.-M. Lin, M.-S. Min, and M. Sumida
  • Organizer: International Symposium “Frontiers in Amphibian Biology”
  • Place of Presentation: Hiroshima University, Higashihiroshima
• [Presentation] Molecular cytogenetic studies on the process of genomic and chromosomal evolution in Xenopus laevis after WGD and the origin and evolution of sex chromosomes in anuran species (2014)
  • Author(s): Uno, Y., C. Nishida, C. Takagi, T. Igawa, N. Ueno, M. Sumida and Y. Matsuda
  • Organizer: International Symposium “Frontiers in Amphibian Biology”
  • Place of Presentation: Hiroshima University, Higashihiroshima
• [Presentation] Discovery and phylogenetic distribution of a short interspersed nuclear element (SINE) subgroup in neobatrachian frogs (2014)
  • Author(s): Kurabayashi, A., N. Furuno, M. Sumida, H. Mizuno, K. Ohshima, and M. Vences
  • Organizer: International Symposium “Frontiers in Amphibian Biology”
  • Place of Presentation: Hiroshima University, Higashihiroshima
• [Presentation] Inbreeding coefficient and genetic relationship of seven strains of Xenopus tropicalis inferred from genome wide genotyping of 54 microsatellite loci (2014)
  • Author(s): Watanabe, A., T. Igawa, A. Kashiwagi, A. Suzuki, A. Kurabayashi, T. Fujii, and M. Sumida
  • Organizer: International Symposium “Frontiers in Amphibian Biology”
  • Place of Presentation: Hiroshima University, Higashihiroshima
• [Presentation] カエル亜目におけるSINE(short interspersed nuclear elements)の発見と系統分布 (2013)
  • Author(s): 倉林敦・古野伸明・住田正幸・水野英明・大島一彦・M. Vences
  • Organizer: 日本爬虫両棲類学会第52回大会
  • Place of Presentation: 東海大学、札幌
• [Presentation] Evolutionary relationships and postmating isolation among Fejervarya species from Lesser Sunda, Indonesia and other Asian countries revealed by mtDNA Cyt b gene sequences and crossing experiments (2013)
  • Author(s): Hasan, M., N. Kurniawan, A. Soewondo, M. M. Islam, T. Igawa, W. M. M. Nalley, M. Matsui and M. Sumida
  • Organizer: 日本爬虫両棲類学会第52回大会
  • Place of Presentation: 東海大学、札幌
• [Presentation] A new species of genus Hoplobatrachus (Anura, Dicroglossidae) from the coastal belt of Bangladesh (2012)
  • Author(s): Hasan, M., M. Kuramoto, M. M. Islam, M. S. Alam, M. M. R. Khan and M. Sumida
  • Organizer: 日本爬虫両棲類学会第51回大会
  • Place of Presentation: 愛知学泉大学 豊田市
  • Year and Date: 2012-11-11
• [Presentation] 南西諸島の絶滅危惧両生類における遺伝的分化と飼育下繁殖 (2012)
  • Author(s): 住田正幸・井川武・イスラム モハメド マフィズル・新谷望・掛橋竜祐・菅原弘貴・西谷琢磨・倉林敦・大海昌平・勝連盛輝・田戸美雪・藤井保
  • Organizer: 日本爬虫両棲類学会第51回大会
  • Place of Presentation: 愛知学泉大学 豊田市
  • Year and Date: 2012-11-10
• [Presentation] アマミイシカワガエルの飼育下における温度条件と遺伝的要因が個体生存率に与える影響 (2012)
  • Author(s): 新谷望・井川武・住田正幸
  • Organizer: 日本爬虫両棲類学会第51回大会
  • Place of Presentation: 愛知学泉大学 豊田市
  • Year and Date: 2012-11-10
• [Presentation] 次世代モデル動物ネッタイツメガエルとその輸送法の改善について (2012)
  • Author(s): 倉林敦・掛橋竜祐・田澤一朗・住田正幸・原本悦和・大嶋友美・伊藤弓弦
  • Organizer: 日本爬虫両棲類学会第51回大会
  • Place of Presentation: 愛知学泉大学 豊田市
  • Year and Date: 2012-11-10
• [Presentation] Genetic divergence and postmating isolation among the brown frogs from Ryukyu Archipelago, Japan, elucidated by allozyme and mitochondrial gene sequence analyses, crossing experiments and cytological observations (2012)
  • Author(s): Islam, M. M., H. Tokimitsu, M. Uchida, M. Iwanari, T. Fujii, and M. Sumida
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 九州大学 福岡市
  • Year and Date: 2012-09-26
• [Presentation] マイクロサテライトマーカーを用いた絶滅危惧種および天然記念物イボイモリの遺伝的集団構造の解明 (2012)
  • Author(s): 井川武・菅原弘貴・本田正尚・富永篤・大海昌平・勝連盛輝・太田英利・松井正文・松井正文
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 九州大学 福岡市
  • Year and Date: 2012-09-25
• [Presentation] 中央琉球産絶滅危惧両生類5種の遺伝的分化と飼育下繁殖 (2012)
  • Author(s): 住田正幸・井川武・イスラム モハメド マフィズル・新谷望・掛橋竜祐・菅原弘貴・倉林敦・大海昌平・勝連盛輝・田戸美雪・藤井保
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪大学 豊中市
  • Year and Date: 2012-09-15
• [Presentation] Postmating isolation in six species of three genera (Hoplobatrachus, Euphlyctis and Fejervarya) from family Dicroglossidae (Anura), with special reference to spontaneous production of allotriploids (2012)
  • Author(s): Alam, M. S., M. M. Islam, M. M. R. Khan, M. Hasan, R. Wanichanon and M. Sumida
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪大学 豊中市
  • Year and Date: 2012-09-15
• [Presentation] 日本産トノサマガエル種群の分布重複域における浸透交雑と30年間で生じた分布変化 (2012)
  • Author(s): 小巻翔平・倉林敦・イスラム モハメド マフィズル・東城幸治・住田正幸
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪大学 豊中市
  • Year and Date: 2012-09-15
• [Presentation] A new species of genus Hoplobatrachus (Anura, Dicroglossidae) from the coastal belt of Bangladesh (2012)
  • Author(s): Hasan, M., M. Kuramoto, M. M. Islam, M. S. Alara, M. M. R. Khan and M. Sumida
  • Organizer: 7th World Congress of Herpetology
  • Place of Presentation: University of British Columbia, Vancouver, Canada
  • Year and Date: 2012-08-12
• [Presentation] Complete mitochondrial sequences of the three Japanese Babina (Ranidae, Anura) species and the phylogenetic position among Ranidae (2012)
  • Author(s): Kakehashi, R., A. Kurabayashi, S. Oumi, S. Katsuren, M. Hoso, and M. Sumida
  • Organizer: 7th World Congress of Herpetology
  • Place of Presentation: University of British Columbia, Vancouver, Canada
  • Year and Date: 2012-08-12
• [Presentation] Genetic divergence and postmating isolation among the brown frogs from Ryukyu Archipelago, Japan, elucidated by allozyme and mitochondrial gene sequence analyses, crossing experiments and cytological observations (2012)
  • Author(s): Islam, M. M., H. Tokimitsu, M. Uchida, M. Iwanari, T. Fujii, and M. Sumida
  • Organizer: 7th World Congress of Herpetology
  • Place of Presentation: University of British Columbia, Vancouver, Canada
  • Year and Date: 2012-08-11
• [Presentation] Artificial and natural breeding of the endangered frog species Odorrana ishikawae and Odorrana splendida, analysis of genetic diversity, and usage as resource materials : Case study of fauna conservation in Japan (2012)
  • Author(s): Sumida, M., N. Satou, M. M. Islam, T. Igawa, A. Kurabayashi, K. Ukena, S. Oumi, S. Katsuren, and T. Fujii
  • Organizer: 7th World Congress of Herpetology
  • Place of Presentation: University of British Columbia, Vancouver, Canada
  • Year and Date: 2012-08-11
• [Presentation] Postmating isolation in six species of three genera (Hoplobatrachus, Euphlyctis and Fejervarya)from family Dicroglossidae (Anura), with special reference to spontaneous production of allotriploids (2012)
  • Author(s): Alam, M. S., M. M. Islam, M. M. R. Khan, M. Hasan, R. Wanichanon and M. Sumida
  • Organizer: 7th World Congress of Herpetology
  • Place of Presentation: University of British Columbia, Vancouver, Canada
  • Year and Date: 2012-08-11
• [Presentation] From Antarctica or Asia? New colonization scenario for Australian-New Guinean narrow mouth toads suggested from the findings on a mysterious genus Gastrophrynoides (2012)
  • Author(s): Kurabayashi, A., M. Matsui, Daicus, M. B., H. S. Yong, N. Ahmad, A. Sudin, M. Kuramoto, A. Hamidy, and M. Sumida
  • Organizer: 7th World Congress of Herpetology
  • Place of Presentation: University of British Columbia, Vancouver, Canada
  • Year and Date: 2012-08-10
• [Presentation] Distributional change and epidemic introgression in overlapping areas of Japanese pond frog species over 30 years (2012)
  • Author(s): Komaki, S., A. Kurabayashi, M. M. Islam, K. Tojo, and M. Sumida
  • Organizer: 7th World Congress of Herpetology
  • Place of Presentation: University of British Columbia, Vancouver, Canada
  • Year and Date: 2012-08-09
• [Presentation] ウシガエルはどこから来て、なぜ駆逐されないのか?:侵入成功と個体群存続における遺伝的要因の調査 (2012)
  • Author(s): 徳増大輔・井川武・ミン ミースク・住田正幸
  • Organizer: 日本爬虫両棲類学会第51回大会
  • Place of Presentation: 愛知学泉大学 豊田市
• [Presentation] 両生類絶滅危惧種の飼育下繁殖による保全の試み
  • Author(s): 住田正幸・井川武・Islam M. M.・倉林敦・Alam, M. S.・掛橋竜祐・田戸美雪・新谷望・菅原弘貴・西谷琢磨・大海昌平・勝連盛輝・藤井保
  • Organizer: 日本遺伝学会第85回大会
  • Place of Presentation: 慶応大学、横浜
• [Presentation] Description of two new species of Microhyla (Anura: Microhylidae) from Bangladesh
  • Author(s): Hasan, M., M. M. Islam, M. Kuramoto, A. Kurabayashi and M. Sumida
  • Organizer: 日本遺伝学会第85回大会
  • Place of Presentation: 慶応大学、横浜
• [Presentation] Genetic divergence and postmating isolation between endangered Holst’s frog and Otton frog from the Ryukyu Archipelago, Japan, elucidated by allozyme and mitochondrial gene sequence analyses, crossing experiments and cytological observations
  • Author(s): Islam, M. M., R. Kakehashi, H. Koide, A. Kurabayasi, S. Oumi, S. Katsuren, T. Fujii, and M. Sumida
  • Organizer: 日本遺伝学会第85回大会
  • Place of Presentation: 慶応大学、横浜
• [Presentation] アフリカツメガエルのゲノム倍数化過程に生じた染色体再配列と無尾両生類における性染色体の起源とその進化に関する分子細胞遺伝学的研究
  • Author(s): 宇野好宣・西田千鶴子・高木知世・井川武・上野直人・住田正幸・松田洋一
  • Organizer: 日本遺伝学会第85回大会
  • Place of Presentation: 慶応大学、横浜
• [Presentation] 絶滅危惧種イボイモリの遺伝的多様性と飼育下繁殖
  • Author(s): 住田正幸・井川武・菅原弘貴・田戸美雪・西谷琢磨・倉林敦・Islam M. M.・大海昌平・勝連盛輝・藤井保
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山大学、岡山市
• [Presentation] 東アジア産トノサマガエル種群の系統関係と遺伝子浸透
  • Author(s): 小巻翔平・井川武・倉林敦・Mi-Sook Min・Si-Min Lin・住田正幸
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山大学、岡山市
• [Presentation] 透明ガエルの遺伝様式と真皮色素細胞の組織学的観察
  • Author(s): 住田正幸・M. M. Islam・井川 武・倉林 敦・古川友加里・佐野尚美・藤井 保・吉崎範夫
  • Organizer: 日本爬虫両棲類学会第52回大会
  • Place of Presentation: 東海大学、札幌
• [Presentation] Genetic divergence and postmating isolation among several brown frog species from Honshu and Ryukyu of Japan, and Mongolia elucidated by mitochondrial gene sequence analyses, crossing experiments and subsequent cytological observations of their hybrids and controls
  • Author(s): Islam, M. M., S. Oumi, H. Ida and M. Sumida
  • Organizer: 日本爬虫両棲類学会第52回大会
  • Place of Presentation: 東海大学、札幌
• [Presentation] 両生類モデル動物ネッタイツメガエル6系統における近交度及び遺伝的関係の解明
  • Author(s): 渡辺愛・井川武・鈴木厚・柏木昭彦・倉林敦・藤井 保・住田正幸
  • Organizer: 日本爬虫両棲類学会第52回大会
  • Place of Presentation: 東海大学、札幌
• [Presentation] 絶滅危惧種ホルストガエルにおける遺伝的集団構造
  • Author(s): 掛橋竜祐・井川武・住田正幸
  • Organizer: 日本爬虫両棲類学会第52回大会
  • Place of Presentation: 東海大学、札幌
• [Presentation] Ion Torrent PGM を用いたハナサキガエルにおけるマイクロサテライト遺伝子座の単離と集団構造解析
  • Author(s): 井川 武・長岡麻衣・野澤昌文・小巻翔平・藤井 保・住田正幸
  • Organizer: 日本爬虫両棲類学会第52回大会
  • Place of Presentation: 東海大学、札幌
• [Presentation] Captive breeding of endangered amphibian species: A case study of ex situ conservation
  • Author(s): Sumida, M., T. Igawa, M. M. Islam, A. Kurabayashi, M. S. Alam, R. Kakehashi, M. Tado, N. Shintani, H. Sugawara, T. Nishitani, S. Oumi, S. Katsuren, and T. Fujii
  • Organizer: 50th Anniversary Meeting of the Australian Society of Herpetologists
  • Place of Presentation: Greenhills Conference Centre, Canberra Australia
• [Presentation] Description of two new species of Microhyla (Anura: Microhylidae) from Bangladesh
  • Author(s): Hasan, M., M. M. Islam, M. Kuramoto, A. Kurabayashi and M. Sumida
  • Organizer: 50th Anniversary Meeting of the Australian Society of Herpetologists
  • Place of Presentation: Greenhills Conference Centre, Canberra Australia
• [Presentation] A case study of fauna conservation in Japan: Current conservation measures of wild populations, captive breeding, genetic diversity, and potential resource materials in the endangered frog species Odorrana ishikawae and O. splendida
  • Author(s): Sumida, M., M. M. Islam, T. Igawa, A. Kurabayashi, N. Satou, K. Ukena, S. Oumi, S. Katsuren, and T. Fujii
  • Organizer: International Symposium “Frontiers in Amphibian Biology”
  • Place of Presentation: Hiroshima University, Higashihiroshima
  • Invited
• [Presentation] An ex situ conservation effort for several endangered and near-threaten amphibian species from the Ryukyu Archipelago, Japan: Captive breeding of nine anuran species and cryopreservation of sperm for two of them
  • Author(s): Islam, M. M., T. Igawa, A. Kurabayashi, R. Kakehashi, N. Satou, N. Shintani, M. Tado, H. Sugawara, T. Nishitani, M. Uchida, S. Oumi, S. Katsuren, T. Fujii, and M. Sumida
  • Organizer: International Symposium “Frontiers in Amphibian Biology”
  • Place of Presentation: Hiroshima University, Higashihiroshima
• [Remarks] 両生類研究施設多様化機構研究グループ住田研究室
  • URL: http://home.hiroshima-u.ac.jp/~amphibia/sumida/
• [Remarks] http://home.hiroshima-u.ac.jp/~amphibia/sumida/
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/~amphibia/sumida/