Estabishment of disease specific intrahepatic biliary epithelial cells by direct reprogramming
Project/Area Number |
26670376
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Gastroenterology
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Research Institution | Yamagata University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | 形質転換 / 線維芽細胞 / 胆管上皮細胞 / ダイレクトリプログラミング / 遺伝子導入 / アミラーゼ / レトロ・ウイルス / トランスフェクション / レトロウイルス |
Outline of Final Research Achievements |
This study aimed to establish intrahepatic biliary epithelial cells by direc reprogramming method. Briefly, human fibroblast cells were hervested from oral cavity, and cultured. Thereafter, several genes were introduced using retroviral vector. After adequate screeming, cells were evaluated their phenotype, physiologically, biochemically, and molecularly. Our established cells exhibited amylase production, which is regarded as the marker of pancreatic ductal cells. However, our cells did not exhibited the markers as the intrahepatic biliary epithelial cells such as cytokerating 7, bicarbonate production, and expression of biliary transporters. Thus, up to now, our study did not achieve the establishment of aimed cells. Given the fact that pancreatic ductal cells is a similar cells to bile duct cells, further improvement could enables us to develop aimed cells.
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Report
(4 results)
Research Products
(3 results)
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[Journal Article] Regulation of the histamine/VEGF axis by miR-125b during cholestatic liver injury in mice.2014
Author(s)
Meng F, Onori P, Hargrove L, Han Y, Kennedy L, Graf A, Hodges K, Ueno Y, Francis T, Gaudio E, Francis HL.
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Journal Title
Am J Pathol.
Volume: 184
Issue: 3
Pages: 662-73
DOI
Related Report
Peer Reviewed