KAKEN — Research Projects | Researcher Number: 30106330

1. A sutdy for analyzing causative genes for common diseases in urinary tract and male sex organs

Research Project

Research Category	Grant-in-Aid for Scientific Research (A)
Research Field	Urology
Research Institution	Kochi Medical School
Principal Investigator	SHUIN Taro Urology, Kochi Medical School, Professor
Project Period (FY)	2000 – 2001Completed
Keywords	common disease / urinary tract caluculi / BPH / Infantile anomaly in male sex organ / genitourinary cancer / DNA microarray analyses
Research Abstract	there are a many common disease in the genitourinary tract organ. For example, urinary tract calculi (uric acid or, oxalic acid calcium calculi), BHP, ...

Research Products of this project	Publications (37 results)

2. 電気穿孔法を用いる尿路性器癌の遺伝子治療の開発研究

Research Project

Research Category	Grant-in-Aid for Exploratory Research
Research Field	Urology
Research Institution	Yokohama City University
Principal Investigator	穂坂正彦横浜市立大学, 医学部, 教授
Project Period (FY)	1999 – 2000Completed
Keywords	遺伝子治療 / 電気穿孔法 / 前立腺癌 / P53遺伝子 / PS3遺伝子
Research Abstract	本研究では萌芽的な研究としてこの電気穿孔法を、まず動物レベルでの遺伝子導入応用を試み、その基礎的検討を行う。さらに、ヒト遺伝子治療の新しい手段の確立を目指した種々の条件を探る。

Research Products of this project	Publications (3 results)

3. Analysis of spermatogonial proliferation with technique of spermatogonial transplantation-A trial for improving spermatogenic activity

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	Yokohama City University
Principal Investigator	OGAWA Takehiko Yokohama City University, Urology, Lecturer
Project Period (FY)	1999 – 2000Completed
Keywords	Spermatogeneiss / Spermatogonia / Transplantation / Stem cell
Research Abstract	Spermatogonial transplantation has several unique advantages when applied to the study of spermatogenesis. For instance, germ cells and testis environ ...

Research Products of this project	Publications (17 results)

4. Functional Analysis of Steroid hormone orphan receptor in hormone refractory prostate cancer

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	Yokohama City University
Principal Investigator	UEMURA Hiroji Yokohama City University School of Medicine, Department of Urology, Assistant Professor
Project Period (FY)	1999 – 2001Completed
Keywords	Steroid hormone orphan receptor / prostate cancer / transcription factor
Research Abstract	Prostate specific antigen (PSA) has been widely recognized as a most reliable tumor marker. A recent report of immunohistochemistry has demonstrated t ...

Research Products of this project	Publications (14 results)

5. Study of the identification of new genes inducing hormone refractory prostate cancer

Research Project

Research Category	Grant-in-Aid for Scientific Research (B)
Research Field	Urology
Research Institution	Yokohama City University Grant-in Aid for Scientific Research (B)(2)
Principal Investigator	KUBOTA Yoshinobu Yokohama City Univ., School of Medicine, Professor
Project Period (FY)	1999 – 2001Completed
Keywords	Prostate cancer / Hormone Refractory / Differential Display / OSM-? gene / nmt55 gene / Liprin? gene
Research Abstract	In this research, new human genes associated with hormone refractory prostate cancer were tried to be identified by applying differential display PCR ...

Research Products of this project	Publications (18 results)

6. Functional Analysis of the von Hippel-Lindau Disease Tumor Suppressor Gene

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	Yokohama City University
Principal Investigator	YAO Masahiro School of Medicine, Urology, Yokohama City University, Assistant Professor
Project Period (FY)	1998 – 1999Completed
Keywords	VHL gene / tumor suppressor gene / renal cell carcinoma
Research Abstract	We found that, in normal human renal proximal tubule epithelial cells, the steady-state amount of VHL protein is strictly regulated by cell density. T ...

Research Products of this project	Publications (13 results)

7. Studies on a KRAB zinc finger gene, highly expressed in the human testis

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	Yokohama City University
Principal Investigator	TAKEDA Mitsumasa Yokohama City Univ., Urology, assistant professor
Project Period (FY)	1998 – 1999Completed
Keywords	zinc finger gene / germ cell / spermatogenesis / male infertility / testicular tumour
Research Abstract	Six Kruppe1-type zinc finger (ZF) genes were cloned from a seminoma cDNAlibrary. One, ZFS-1, showed highly sequence homology to the ZNF91 KRAB (Kruppe ...

Research Products of this project	Publications (11 results)

8. Study of the development a new type of treatment of urogenital cancer by using surface modified fine metal particles.

Research Project

Research Category	Grant-in-Aid for Scientific Research (B)
Research Field	Urology
Research Institution	Yokohama City University
Principal Investigator	KUBOTA Yoshinobu Yokohama City Univ., School of Med., Asso. Professor
Project Period (FY)	1998 – 2000Completed
Keywords	Fine metal particles / TiO_2 / Photocatalysis / Urogenital Cancer / Cancer treatment
Research Abstract	We tried to develop a new type of treatment of cancer by using specifically surface modified fine metal particles, especially particles coated by TiO_ ...

Research Products of this project	Publications (26 results)

9. ANALYSIS OF TR3 RECEPTOR IN APOPTOSIS OF PROSTATE CANCER AND APPLICATION FOR DIAGNOSIS OF PROSTATE CANCER RECCURENCE

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	YOKOHAMA CITY UNIVERSITY
Principal Investigator	UEMURA Hiroji DEP.UROL., YOKOHAMA CITY UNIVERSITY, ASSIST.PROF
Project Period (FY)	1996 – 1998Completed
Keywords	PROSTATE CANCER / STEROID RECEPTOR / APOPTOSIS
Research Abstract	(1) A role of TR3 orphan receptor in drug-induced prostate apoptosis ...

Research Products of this project	Publications (19 results)

10. Molecular Genetic Study of the VHL Tumor Suppressor Gene in Human Renal Cell Carcinoma

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	Yokohama City University
Principal Investigator	YAO Masahiro Yokohama City University School of Medicine, Department of Urology, Assistant Professor
Project Period (FY)	1996 – 1997Completed
Keywords	VHL gene / renal cell / carcinoma / tumor suppressor / mutation
Research Abstract	We have examined 232 primary sporadic renal cell carcinomas (RCCs) as well as 77 Japanese VHL families for VHL gene mutations. Mutation of the VHL gen ...

Research Products of this project	Publications (12 results)

11. Study of the Identification of new genes associated with development and recurrence of prostate cancer

Research Project

Research Category	Grant-in-Aid for Scientific Research (B)
Research Field	Urology
Research Institution	Yokohama City University
Principal Investigator	KUBOTA Yoshinobu Yokohama City Univ. School of Med., Asso. Professor
Project Period (FY)	1996 – 1998Completed
Keywords	prostate cancer / Telomerase / defferential display / liprin-α2
Research Abstract	Prostate cancer is one of the most common neoplasms in the Western country. Molecular background of this disease is still unclear. Thus, We studied th ...

Research Products of this project	Publications (24 results)

12. Studies on molecularbiological approach of male infertility-Isolation of androgen responsive genes by genomic binding-site method.

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	Yokohama City University
Principal Investigator	TAKEDA Mitsumasa Yokohama City Univ., Urology, assistant professor
Project Period (FY)	1995 – 1997Completed
Keywords	androgen / androgen receptor / androgen responsive gene / epididymis / rat
Research Abstract	To obtain a better understanding of molecular mechanism of androgen action on spermatogenesis and spermiogenesis, we performed genomic binding-site me ...

Research Products of this project	Publications (12 results)

13. Positive Control of Gonadotropin Secretion by Androgen

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	Yokohama City University
Principal Investigator	NOGUCHI Kazumi Yokohama City University, School of Medicine, Associate Professor
Project Period (FY)	1995 – 1997Completed
Keywords	Pituitary gland / Gonadotropin / Testosterone / Positive Control
Research Abstract	We investigated the effects of testosterone (T) on FSH secretion from male rat anterior pituitary cells in cultures at the single cell level. Anterior ...

Research Products of this project	Publications (9 results)

14. Mutational Analysis of the VHL Tumor Suppressor Gene in Sporadic Renal Cell Carcinoma

Research Project

Research Category	Grant-in-Aid for General Scientific Research (C)
Research Field	Urology
Research Institution	Yokohama City University
Principal Investigator	YAO Masahiro Yokohama City University School of medicine, Fuculty Staff
Project Period (FY)	1994 – 1995Completed
Keywords	carcinoma / rsnal cell / VHL / tumor suppressor gene
Research Abstract	We analyzed 47 sporadic renal cell carcinomas for somatic mutations of the von Hippel-Lindau(VHL)tumor suppressor gene by PCR-SSCP and sequencing anal ...

Research Products of this project	Publications (6 results)

15. Analysis of genes involving in reactivation of prostate cancer

Research Project

Research Category	Grant-in-Aid for General Scientific Research (B)
Research Field	Urology
Research Institution	Yokohama City University
Principal Investigator	KUBOTA Yoshinobu Yokohama City Univ., School of Med, Ass.Professor
Project Period (FY)	1994 – 1995Completed
Keywords	Prostate cancer / Reactivation / gene alterations
Research Abstract	The molecular background underlying the progression to reactivation stage of prostate cancer is not yet clear.

Research Products of this project	Publications (17 results)

16. Anew trial of Genetic dlagnosisand treatment of kidney cancer based on the abnormallty in the oncogene and tumor suppressor gene Head invastigator ; Registered invastigators : Resgistered

Research Project

Research Category	Grant-in-Aid for General Scientific Research (C)
Research Field	Urology
Research Institution	Kochi Medical School (1995) Yokohama City University (1993-1994)
Principal Investigator	SHUIN Taro Kochi Medical School professor
Project Period (FY)	1993 – 1995Completed
Keywords	renal cell carcinoma / von Hippel-Lindau gene / p53gene / retinoblastoma gene
Research Abstract	Human cancers develops in a multistep processes. Activation of oncogenes and inactivation of tumor suppressor gene is associated in each process. Our ...

Research Products of this project	Publications (15 results)

17. Intercellular Communication Between Sertoli Cell and Leydig Cell

Research Project

Research Category	Grant-in-Aid for General Scientific Research (C)
Research Field	Urology
Research Institution	Yokohama City University
Principal Investigator	NOGUCHI Kazumi Yokohama City University School of Medicine Associate Professor
Project Period (FY)	1993 – 1994Completed
Keywords	Sertoli Cell / Leydig Cell / Testosterone / Paracrine Control
Research Abstract	In the present study, we demonstrate the existence of a factor which stimulates testosterone production by mature mouse Leydig cells in a spent medium ...

Research Products of this project	Publications (5 results)

18. Application of photoexcited TiO2 semicondoctor to the treatment of bladder cancer

Research Project

Research Category	Grant-in-Aid for Developmental Scientific Research (B)
Research Field	Urology
Research Institution	Yokohama City University
Principal Investigator	KUBOTA Yoshinobu Yokohama City Univ., School of Med, Ass.Professor
Project Period (FY)	1993 – 1995Completed
Keywords	photoexcited semiconductor / TiO2 / Bladder cancer
Research Abstract	A photoexcited titanium dioxide surface has a strong ability to decompose water into hydrogen and oxygen. We have studied this effect in order to use ...

Research Products of this project	Publications (20 results)

19. Bulk collection of epididymal tail spermatozoa by means of electric stimulation

Research Project

Research Category	Grant-in-Aid for General Scientific Research (C)
Research Field	Urology
Research Institution	Yokohama City University
Principal Investigator	KINOSHITA Yuzo Yokohama City University, School of Medicine, Associate Professor
Project Period (FY)	1992 – 1994Completed
Keywords	Epididymis / Electric stimulation / Spermatozoa
Research Abstract	In 12-week-old Wistar rats, 70V,250 mu s pulse width, 20 Hz electric stimulation on the epididymal tail for 30 s resulted in a recovery of 10.5(]SY.+- ...

Research Products of this project	Publications (3 results)

20. 前立腺癌の分化度と男性ホルモン作用機構上の遺伝子発現の差に基づく悪性度診断

Research Project

Research Category	Grant-in-Aid for General Scientific Research (B)
Research Field	Urology
Research Institution	Yokohama City University
Principal Investigator	穂坂正彦横浜市立大学, 医学部, 教授
Project Period (FY)	1992 – 1994Completed
Keywords	前立腺癌 / 5alpha-reductase / LNCaP細胞 / 5α-reductase
Research Abstract	ラットおける5alpha-reductaseの遺伝子発現をRT-PCRによって作成したcADNA probe を用いて検討した。ラットにおいてはnorthern blot analysisによって、5alpha-reductaseのシグナルを確認することができ、このシグナルはtestosterone ...

Research Products of this project	Publications (1 results)

Project Period (Start Year [FY])

1. A sutdy for analyzing causative genes for common diseases in urinary tract and male sex organs Research Project

SHUIN Taro Urology, Kochi Medical School, Professor

2. 電気穿孔法を用いる尿路性器癌の遺伝子治療の開発研究 Research Project

穂坂 正彦 横浜市立大学, 医学部, 教授

3. Analysis of spermatogonial proliferation with technique of spermatogonial transplantation-A trial for improving spermatogenic activity Research Project

OGAWA Takehiko Yokohama City University, Urology, Lecturer

4. Functional Analysis of Steroid hormone orphan receptor in hormone refractory prostate cancer Research Project

UEMURA Hiroji Yokohama City University School of Medicine, Department of Urology, Assistant Professor

5. Study of the identification of new genes inducing hormone refractory prostate cancer Research Project

KUBOTA Yoshinobu Yokohama City Univ., School of Medicine, Professor

6. Functional Analysis of the von Hippel-Lindau Disease Tumor Suppressor Gene Research Project

YAO Masahiro School of Medicine, Urology, Yokohama City University, Assistant Professor

7. Studies on a KRAB zinc finger gene, highly expressed in the human testis Research Project

TAKEDA Mitsumasa Yokohama City Univ., Urology, assistant professor

8. Study of the development a new type of treatment of urogenital cancer by using surface modified fine metal particles. Research Project

KUBOTA Yoshinobu Yokohama City Univ., School of Med., Asso. Professor

9. ANALYSIS OF TR3 RECEPTOR IN APOPTOSIS OF PROSTATE CANCER AND APPLICATION FOR DIAGNOSIS OF PROSTATE CANCER RECCURENCE Research Project

UEMURA Hiroji DEP.UROL., YOKOHAMA CITY UNIVERSITY, ASSIST.PROF

10. Molecular Genetic Study of the VHL Tumor Suppressor Gene in Human Renal Cell Carcinoma Research Project

YAO Masahiro Yokohama City University School of Medicine, Department of Urology, Assistant Professor

11. Study of the Identification of new genes associated with development and recurrence of prostate cancer Research Project

KUBOTA Yoshinobu Yokohama City Univ. School of Med., Asso. Professor

12. Studies on molecularbiological approach of male infertility-Isolation of androgen responsive genes by genomic binding-site method. Research Project

TAKEDA Mitsumasa Yokohama City Univ., Urology, assistant professor

13. Positive Control of Gonadotropin Secretion by Androgen Research Project

NOGUCHI Kazumi Yokohama City University, School of Medicine, Associate Professor

14. Mutational Analysis of the VHL Tumor Suppressor Gene in Sporadic Renal Cell Carcinoma Research Project

YAO Masahiro Yokohama City University School of medicine, Fuculty Staff

15. Analysis of genes involving in reactivation of prostate cancer Research Project

KUBOTA Yoshinobu Yokohama City Univ., School of Med, Ass.Professor

16. Anew trial of Genetic dlagnosisand treatment of kidney cancer based on the abnormallty in the oncogene and tumor suppressor gene Head invastigator ; Registered invastigators : Resgistered Research Project

SHUIN Taro Kochi Medical School professor

17. Intercellular Communication Between Sertoli Cell and Leydig Cell Research Project

NOGUCHI Kazumi Yokohama City University School of Medicine Associate Professor

18. Application of photoexcited TiO2 semicondoctor to the treatment of bladder cancer Research Project

KUBOTA Yoshinobu Yokohama City Univ., School of Med, Ass.Professor

19. Bulk collection of epididymal tail spermatozoa by means of electric stimulation Research Project

KINOSHITA Yuzo Yokohama City University, School of Medicine, Associate Professor

20. 前立腺癌の分化度と男性ホルモン作用機構上の遺伝子発現の差に基づく悪性度診断 Research Project

穂坂 正彦 横浜市立大学, 医学部, 教授

1. A sutdy for analyzing causative genes for common diseases in urinary tract and male sex organs

Research Project

2. 電気穿孔法を用いる尿路性器癌の遺伝子治療の開発研究

Research Project

穂坂正彦横浜市立大学, 医学部, 教授

3. Analysis of spermatogonial proliferation with technique of spermatogonial transplantation-A trial for improving spermatogenic activity

Research Project

4. Functional Analysis of Steroid hormone orphan receptor in hormone refractory prostate cancer

Research Project

5. Study of the identification of new genes inducing hormone refractory prostate cancer

Research Project

6. Functional Analysis of the von Hippel-Lindau Disease Tumor Suppressor Gene

Research Project

7. Studies on a KRAB zinc finger gene, highly expressed in the human testis

Research Project

8. Study of the development a new type of treatment of urogenital cancer by using surface modified fine metal particles.

Research Project

9. ANALYSIS OF TR3 RECEPTOR IN APOPTOSIS OF PROSTATE CANCER AND APPLICATION FOR DIAGNOSIS OF PROSTATE CANCER RECCURENCE

Research Project

10. Molecular Genetic Study of the VHL Tumor Suppressor Gene in Human Renal Cell Carcinoma

Research Project

11. Study of the Identification of new genes associated with development and recurrence of prostate cancer

Research Project

12. Studies on molecularbiological approach of male infertility-Isolation of androgen responsive genes by genomic binding-site method.

Research Project

13. Positive Control of Gonadotropin Secretion by Androgen

Research Project

14. Mutational Analysis of the VHL Tumor Suppressor Gene in Sporadic Renal Cell Carcinoma

Research Project

15. Analysis of genes involving in reactivation of prostate cancer

Research Project

16. Anew trial of Genetic dlagnosisand treatment of kidney cancer based on the abnormallty in the oncogene and tumor suppressor gene Head invastigator ; Registered invastigators : Resgistered

Research Project

17. Intercellular Communication Between Sertoli Cell and Leydig Cell

Research Project

18. Application of photoexcited TiO2 semicondoctor to the treatment of bladder cancer

Research Project

19. Bulk collection of epididymal tail spermatozoa by means of electric stimulation

Research Project

20. 前立腺癌の分化度と男性ホルモン作用機構上の遺伝子発現の差に基づく悪性度診断

Research Project

穂坂正彦横浜市立大学, 医学部, 教授