KAKEN — Research Projects | Researcher Number: 40190243

1. Elucidation of molecular mechanisms underlying development of malignant tumors with loss-of-function mutations of UTX, a histone modifier, mainly focusing on urological tumors.

Research Project

Research Category	Grant-in-Aid for Scientific Research (B)
Review Section	Basic Section 50010:Tumor biology-related
Research Institution	Tokyo Women's Medical University
Principal Investigator	本田浩章東京女子医科大学, 医学部, 教授
Project Period (FY)	2023-04-01 – 2027-03-31Granted
Keywords	ヒストン修飾 / UTX, UTY / 泌尿器科系腫瘍 / 炎症性経路
Outline of Research at the Start	ヒストン修飾は遺伝子発現制御を介して組織恒常性に重要な役割を果たし、その逸脱は発がんに関与する。我々は悪性腫瘍で変異を認めるヒストン修飾因子UTXに注目し、変異頻度が最も高い膀胱がんを対象として膀胱特異的にUTXを欠失したマウスを作製し解析した結果、UTX欠失は炎症経路を活性化して発がんに関与するこ ...

2. GWASによる修飾遺伝子変異を加えた日本人ADPKD重症度基準の構築

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Review Section	Basic Section 56030:Urology-related
Research Institution	Juntendo University
Principal Investigator	武藤智順天堂大学, 医学部, 教授
Project Period (FY)	2022-04-01 – 2025-03-31Granted
Keywords	ADPKD / 常染色体優性多発性嚢胞腎 / 重症度分類 / 遺伝子変異
Outline of Research at the Start	本研究では日本人ADPKDにおける原因遺伝子変異の解析、ゲノムワイド関連解析（Genome Wide Association studies: GWAS）による修飾遺伝子変異の同定、遺伝および臨床情報の病勢進行への関与の解明を行い、日本人ADPKDの重症度分類を構築する。
Outline of Annual Research Achievements	常染色体優性多発性嚢胞腎（Autosomal Dominant Polycystic Kidney Disease; ADPKD）は約1,000人に1人と最も頻度が高い遺伝性腎疾患である。末期腎不全にいたるのは約半数であり、適切なリスク分類による重症度判定は、患者の治療方針決定のため欠かせない。さら ...
Current Status of Research Progress	2: Research has progressed on the whole more than it was originally planned.

Research Products of this project	Journal Article (2 results, of which Peer Reviewed: 2 results) Presentation (2 results)

3. Search for biomarkers on immunotherapy for metastatic renal cancer by using circulating tumor cells and repertoire analysis

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Review Section	Basic Section 56030:Urology-related
Research Institution	Juntendo University
Principal Investigator	Nagata Masayoshi 順天堂大学, 医学部, 准教授
Project Period (FY)	2019-04-01 – 2022-03-31Completed
Keywords	腎細胞がん / 免疫チェックポイント阻害薬 / 血中循環腫瘍細胞 / レパトア解析 / 抗CTLA-4抗体 / 抗PD-1抗体 / 転移性腎細胞がん / イピリムマブ / ニボルマブ / 末梢血レパトア解析
Outline of Research at the Start	当科で加療を受ける一次治療のipilimumabおよびnivolumab併用療法、また2次治療のnivolumab治療のIO治療を施行される進行性腎がん患者から、腫瘍側の解析としてCTCを回収して用い、薬剤の標的分子発現やがん抗原、とくにPD-L1分子などの免疫関連分子発現を解析する。また患者側の免 ...
Outline of Final Research Achievements	Circulating tumor cells (CTC) were collected from patients with immune checkpoint inhibitor therapy (IO) for advanced renal cell carcinoma and analyze ...

Research Products of this project	Presentation (1 results, of which Invited: 1 results)

4. Whole genome sequence analysis of ADPKD

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Review Section	Basic Section 56030:Urology-related
Research Institution	Juntendo University
Principal Investigator	Horie Shigeo 順天堂大学, 医学(系)研究科(研究院), 教授
Project Period (FY)	2018-04-01 – 2021-03-31Completed
Keywords	多発性嚢胞腎 / ADPKD / PKD1 / PKD2 / 生殖細胞系列 / 常染色体優性多発性嚢胞腎 / 生殖細胞系列変異 / 遺伝子解析 / 次世代シークエンサー / 全ゲノムシークエンシング / WGS
Outline of Final Research Achievements	Analysis of germline mutations and its association with clinical severity was examined in patients with ADPKD. DNA analysis of 258 patients with ADPKD ...

Research Products of this project	Journal Article (26 results, of which Int'l Joint Research: 3 results, Peer Reviewed: 18 results, Open Access: 3 results) Presentation (43 results, of which Int'l Joint Research: 18 results) Book (2 results)

5. 泌尿器転移癌患者の循環血液中癌細胞の遺伝子解析

Research Project

Research Category	Grant-in-Aid for JSPS Fellows
Research Field	Urology
Research Institution	Juntendo University
Principal Investigator	堀江重郎順天堂大学, 医学(系)研究科(研究院), 教授
Project Period (FY)	2017-11-10 – 2020-03-31Completed
Keywords	血中循環腫瘍細胞 / 去勢抵抗性前立腺癌 / Precision medicine / PSMA / バイオマーカー
Outline of Annual Research Achievements	CRPCを含む進行前立腺がん86例へ、治療経過で継時的にCTC解析を行なった。CTC回収方法は、抗体蛍光染色によるCytoQuest(Abnova社)、抗体磁気ビーズによるAdnaTest(Qiagen社)を用いた。AR-V7(androgen receptor splicing variant 7 ...

Research Products of this project	Journal Article (10 results, of which Int'l Joint Research: 9 results, Peer Reviewed: 8 results, Open Access: 9 results) Presentation (12 results, of which Int'l Joint Research: 9 results, Invited: 1 results)

6. Development of personalized medicine for prostate cancer by TP53 signature diagnostic method

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	Juntendo University
Principal Investigator	Kato Shunsuke 順天堂大学, 医学(系)研究科(研究院), 教授
Project Period (FY)	2016-04-01 – 2019-03-31Completed
Keywords	TP53 / 前立腺がん / 発現プロファイル / 予後予測バイオマーカー / 予後予測診断 / p53 / 予後予測
Outline of Final Research Achievements	Using 50 clinical specimens of prostate cancer, the usefulness of the gene expression profile TP53 signature as a prognostic biomarker was evaluated. ...

Research Products of this project	Journal Article (5 results, of which Int'l Joint Research: 1 results, Peer Reviewed: 5 results, Open Access: 4 results)

7. Effect of testosterone replacement treatment on metabolic and vessel factors in patients with LOH syndrome

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	Juntendo University
Principal Investigator	Tsujimura Akira 順天堂大学, 医学部, 教授
Project Period (FY)	2015-04-01 – 2018-03-31Completed
Keywords	テストステロン補充療法 / LOH症候群 / 動脈硬化 / 性機能障害 / テストステロン / 補充療法 / LOH症状 / メタボリックシンドローム / 勃起能 / 排尿機能 / 更年期症状
Outline of Final Research Achievements	Atherosclerosis is a systematic disease in which plaque builds up inside the arteries, and can lead to serious problems related to QOL. We measured ba ...

Research Products of this project	Journal Article (3 results) Presentation (10 results, of which Int'l Joint Research: 1 results, Invited: 7 results)

8. The development of the generative model for the renal tissue of ADPKD by juman iPS cell

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	Juntendo University
Principal Investigator	HORIE SHIGEO 順天堂大学, 医学(系)研究科(研究院), 教授
Project Period (FY)	2015-04-01 – 2018-03-31Completed
Keywords	常染色体優性多発性嚢胞腎 / modifier gene / エクソンシークエンス / トルバプタン / 総腎容積 / ADPKD / iPS / 多発性嚢胞腎 / iPS細胞
Outline of Final Research Achievements	ADPKD (Autosomal Dominant Polycystic Kidney Disease) is a most frequent hereditary renal disease. Even within the same family, the age leading to end- ...

Research Products of this project	Journal Article (6 results, of which Int'l Joint Research: 1 results, Peer Reviewed: 6 results, Open Access: 4 results) Presentation (3 results, of which Int'l Joint Research: 1 results)

9. Functional analysis of urinary exosome in ADPKD

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	Juntendo University (2013-2014) Teikyo University (2012)
Principal Investigator	HORIE Shigeo 順天堂大学, 医学(系)研究科(研究院), 教授
Project Period (FY)	2012-04-01 – 2015-03-31Completed
Keywords	ADPKD / 多発性嚢胞腎 / exosome / microRNA / 尿中microRNA / microvesicle / LC/MS法 / 尿中バイオマーカー / 奇形学
Outline of Final Research Achievements	Urinary exosome was isolated from either CKD Gstage4 or ADPKD patients upon consent, and the identified microRNA was compared between these 2 groups. ...

Research Products of this project	Journal Article (24 results, of which Peer Reviewed: 14 results, Acknowledgement Compliant: 5 results) Presentation (13 results) Book (3 results)

10. Scientific Support Programs for Cancer Research Grant-in-Aid for Scientific Research on Innovative Areas

Research Project

Research Category	Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Research Institution	The University of Tokyo
Principal Investigator	IMAI Kohzoh (2012-2015) 東京大学, 医科学研究所, 特任研究員中村祐輔 (2010-2011) 東京大学, 医科学研究所, 教授
Project Period (FY)	2010-04-01 – 2016-03-31Completed
Keywords	若手支援 / 国際研究者交流 / 遺伝子改変マウス / 病理形態学 / ゲノムコーホート研究 / 次世代分子解析技術 / アカデミア創薬 / ゲノム・エピゲノム解析支援 / 個別化がん予防 / 国際交流 / ATL発症予防 / 分子疫学コーホート研究 / がん化学予防 / ATL発がん機序 / ATL発症リスク / がん分子標的薬剤
Outline of Final Research Achievements	We started this project first supporting scientists who aim to overcome cancer, and from 2014 we extended our support to scientists in life sciences. ...
Project Area	がん研究分野の特性等を踏まえた支援活動

Research Products of this project	Int'l Joint Research (6 results) Journal Article (815 results, of which Int'l Joint Research: 24 results, Peer Reviewed: 805 results, Open Access: 62 results, Acknowledgement Compliant: 64 results) Presentation (640 results, of which Int'l Joint Research: 30 results, Invited: 111 results) Book (66 results) Remarks (43 results) Patent(Industrial Property Rights) (14 results, of which Overseas: 2 results)

Research Products of this project

Int'l Joint Research (6 results) Journal Article (815 results, of which Int'l Joint Research: 24 results, Peer Reviewed: 805 results, Open Access: 62 results, Acknowledgement Compliant: 64 results) Presentation (640 results, of which Int'l Joint Research: 30 results, Invited: 111 results) Book (66 results) Remarks (43 results) Patent(Industrial Property Rights) (14 results, of which Overseas: 2 results)

11. Comprehensive analysis of urinary exosomes in ADPKD

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	Teikyo University
Principal Investigator	HORIE Shigeo 帝京大学, 医学部, 教授
Project Period (FY)	2009 – 2011Completed
Keywords	ADPKD / exosome / 尿中分泌細胞小体 / 多発性嚢胞腎 / Liquid Chronatography/Mass Spectrometry / LC/MS
Research Abstract	We have isolated urinary exosomes in the control and patients with ADPKD. By utilizing liquid chromatography/mass spectrometry, specific peptides were ...

Research Products of this project	Journal Article (20 results, of which Peer Reviewed: 2 results) Presentation (15 results) Book (7 results)

12. Development of Molecular target drug for ADPKD

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	Teikyo University
Principal Investigator	HORIE Shigeo Teikyo University, School of Medicine, Department Urology, Chairman & professor
Project Period (FY)	2005 – 2006Completed
Keywords	ADPKD / Angiotensin receptor blocker / ω3 unsaturated fatty acid
Research Abstract	Purpose: It is well known that the administration of soy-bean dramatically reduces both tubular and interstitial pathology in the Han : SPRD-cy rat mo ...

Research Products of this project	Journal Article (18 results)

13. The risk evaluation and chemoprevention of the prostate cancer

Research Project

Research Category	Grant-in-Aid for Scientific Research on Priority Areas
Review Section	Biological Sciences
Research Institution	University of Tsukuba
Principal Investigator	AKAZA Hideyuki University of Tsukuba
Project Period (FY)	2005 – 2009Completed
Keywords	がん / ゲノム / 食品 / 大豆イソフラボン / 細菌 / 癌
Research Abstract	The patients with prostate cancer were proved to have low equol-producing ability to metabolize daidzein to equol. For the first time, by the stool cu ...

Research Products of this project	Journal Article (65 results, of which Peer Reviewed: 54 results) Presentation (22 results) Book (1 results)

14. Gene analysis and molecular drug discovery of ADPKD

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	Teikyo University
Principal Investigator	HORIE Shigeo Teikyo Univ, Dept. of Urology. School of Medicine, Professor and Chairman
Project Period (FY)	2003 – 2004Completed
Keywords	ADPKD / polycystin-1 / endothelin-1 / MAPK / PWV / vascular endothelial function
Research Abstract	In the Pkd1^<-/-> kidney, the renal parenchyma of Pkd1^<-/-> had numerous cysts. The hearts of Pkd1^<-/-> had a double-outlet right ventricle (DORV) a ...

Research Products of this project	Journal Article (10 results) Publications (6 results)

15. The involvement of mast cell in the progression of bladder cancer

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	Teikyo University
Principal Investigator	MUTO Satoru Teikyo University, School of Medicine, Department Urology, Associate Professor
Project Period (FY)	2003 – 2005Completed
Keywords	bladder cancer / inflammation / TNF / ras
Research Abstract	The rasH2 transgenic mice carry human c-Ha-ras proto-oncogene, and are highly susceptible to chemical carcinogenesis. Previous studies showed that the ...

Research Products of this project	Journal Article (6 results) Publications (1 results)

16. Establishment of evidence based antibacterial chemotherapy

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	Teikyo University (2003-2004) Kobe University (2001-2002)
Principal Investigator	OKADA Hiroshi Teikyo Universitt, Department of Urology., School of Medicine, Associate professor
Project Period (FY)	2001 – 2004Completed
Keywords	UTI / Susceptihility / Bacteria
Research Abstract	A new automated flow cytometry based urine bacteria analyzer, UBA, was developed. We assessed the UBA for linearity of measurement, reproducibility of ...

Research Products of this project	Journal Article (2 results) Publications (1 results)

17. Study of the molecular pathology of ADPKD

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	Kyorin University (2002) The University of Tokyo (2001)
Principal Investigator	HORIE Shigeo Kyorin University, School of Medicine, Associate Professor
Project Period (FY)	2001 – 2002Completed
Keywords	Pkd1 / ADPKD / Knock-out mice / microarray
Research Abstract	We analyzed the comprehensive gene expression analysis of Pkd1^%, using cDNA microarray. We extracted the mRNA from E14.5 fetus kidney, and applied mR ...

Research Products of this project	Publications (3 results)

18. The correlation between expression of N-cadherin and cadherin-11, and both cancer invasion and metastasis in prostate cancer

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	The University of Tokyo
Principal Investigator	KYOICHI Tomita The University of Tokyo, The University of Tokyo Faculty of Medicine, Lecturer
Project Period (FY)	2001 – 2002Completed
Keywords	prostate cancer / N-cadherin / cadherin-11 / invasion / metastais
Research Abstract	We report on the interaction of both N-cadherin and cadherin-11 between cultured prostate cancer cells and cultured stroma cells and the immunohistoch ...

19. CLINICAL APPLICATION OF GENE THERAPY FOR UROLOGICAL MALIGNANCIES

Research Project

Research Category	Grant-in-Aid for Scientific Research (A)
Research Field	Urology
Research Institution	THE UNIVERSITY OF TOKYO
Principal Investigator	KITAMURA Tadaichi The University of Tokyo, Faculty of Medicin, PROFESSOR
Project Period (FY)	1999 – 2001Completed
Keywords	dendritic cells / gene therapy / HGF / urological malignacies
Research Abstract	The aim of this study was to develop and promote the clinical application of gene therapy for the urologicall malignancies. We performed research on t ...

Research Products of this project	Publications (16 results)

20. MAKING OF MOUSE MODEL OF ADPKD AND GENE THERAPY

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Urology
Research Institution	The University of Tokyo, Branch Hospital
Principal Investigator	HORIE Shigeo UNIVERSITY OF TOKYO BRANCH HOSPITAL, ASSISTANT PROFES.
Project Period (FY)	1998 – 1999Completed
Keywords	ADPKD / Polycystin 2 / ES cell
Research Abstract	The majority of autosomal dominant polycystic kidney disease (ADPKD) is caused by mutations to either the PKD1 or PKD2 gene. Recent studies suggest th ...

Research Products of this project	Publications (3 results)

Project Period (Start Year [FY])

1. Elucidation of molecular mechanisms underlying development of malignant tumors with loss-of-function mutations of UTX, a histone modifier, mainly focusing on urological tumors. Research Project

本田 浩章 東京女子医科大学, 医学部, 教授

2. GWASによる修飾遺伝子変異を加えた日本人ADPKD重症度基準の構築 Research Project

武藤 智 順天堂大学, 医学部, 教授

3. Search for biomarkers on immunotherapy for metastatic renal cancer by using circulating tumor cells and repertoire analysis Research Project

Nagata Masayoshi 順天堂大学, 医学部, 准教授

4. Whole genome sequence analysis of ADPKD Research Project

Horie Shigeo 順天堂大学, 医学(系)研究科(研究院), 教授

5. 泌尿器転移癌患者の循環血液中癌細胞の遺伝子解析 Research Project

堀江 重郎 順天堂大学, 医学(系)研究科(研究院), 教授

6. Development of personalized medicine for prostate cancer by TP53 signature diagnostic method Research Project

Kato Shunsuke 順天堂大学, 医学(系)研究科(研究院), 教授

7. Effect of testosterone replacement treatment on metabolic and vessel factors in patients with LOH syndrome Research Project

Tsujimura Akira 順天堂大学, 医学部, 教授

8. The development of the generative model for the renal tissue of ADPKD by juman iPS cell Research Project

HORIE SHIGEO 順天堂大学, 医学(系)研究科(研究院), 教授

9. Functional analysis of urinary exosome in ADPKD Research Project

HORIE Shigeo 順天堂大学, 医学(系)研究科(研究院), 教授

10. Scientific Support Programs for Cancer Research Grant-in-Aid for Scientific Research on Innovative Areas Research Project

IMAI Kohzoh (2012-2015) 東京大学, 医科学研究所, 特任研究員 中村 祐輔 (2010-2011) 東京大学, 医科学研究所, 教授

11. Comprehensive analysis of urinary exosomes in ADPKD Research Project

HORIE Shigeo 帝京大学, 医学部, 教授

12. Development of Molecular target drug for ADPKD Research Project

HORIE Shigeo Teikyo University, School of Medicine, Department Urology, Chairman & professor

13. The risk evaluation and chemoprevention of the prostate cancer Research Project

AKAZA Hideyuki University of Tsukuba

14. Gene analysis and molecular drug discovery of ADPKD Research Project

HORIE Shigeo Teikyo Univ, Dept. of Urology. School of Medicine, Professor and Chairman

15. The involvement of mast cell in the progression of bladder cancer Research Project

MUTO Satoru Teikyo University, School of Medicine, Department Urology, Associate Professor

16. Establishment of evidence based antibacterial chemotherapy Research Project

OKADA Hiroshi Teikyo Universitt, Department of Urology., School of Medicine, Associate professor

17. Study of the molecular pathology of ADPKD Research Project

HORIE Shigeo Kyorin University, School of Medicine, Associate Professor

18. The correlation between expression of N-cadherin and cadherin-11, and both cancer invasion and metastasis in prostate cancer Research Project

KYOICHI Tomita The University of Tokyo, The University of Tokyo Faculty of Medicine, Lecturer

19. CLINICAL APPLICATION OF GENE THERAPY FOR UROLOGICAL MALIGNANCIES Research Project

KITAMURA Tadaichi The University of Tokyo, Faculty of Medicin, PROFESSOR

20. MAKING OF MOUSE MODEL OF ADPKD AND GENE THERAPY Research Project

HORIE Shigeo UNIVERSITY OF TOKYO BRANCH HOSPITAL, ASSISTANT PROFES.