Blood flow-mediated cell behaviors during vascular remodeling
Project/Area Number |
15K14526
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Developmental biology
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Research Institution | Kyoto University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
KOKUBU Hiroshi 京都大学, 大学院理学研究科, 教授 (50202057)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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Keywords | 血管 / リモデリング / 血流 / トリ杯 / 細胞挙動 / 定量化 / トリ胚 / 血管リモデリング / ニワトリ胚 / 卵黄動脈 / 遺伝子導入 / 血管内皮細胞 / トランスフェクション |
Outline of Final Research Achievements |
When the initial vascular network is formed during embryogenesis, primitive vascular plexus undergoes remodeling. Although it has been known that blood flow is important for the vascular remodeling, how the remodeling proceeds remained poorly known. In this study, toward understanding these mechanisms, we aimed at establishing a novel experimental system in which the blood flow and behaviors of endothelial cells were quantitatively measured in vivo. Using vitelline arteries that undergo massive remodeling in a 2-D structure of early chicken embryos, we succeeded in live imaging of remodeling vasculature. When we artificially stopped/attenuated the blood flow leaving the hear beat unaffected, the remodeling of vitelline artery was compromised. EGFP-labeled endothelial cells displayed dynamic behavior that was dependent on the blood flow. Dynamics of blood flow in micro-environment were also measured, providing a system in which vascular remodeling can be analyzed at the cellular level.
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Report
(4 results)
Research Products
(48 results)
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[Journal Article] Melanosome transfer to keratinocyte in the chicken embryonic skin is mediated by vesicle release associated with Rho-regulated membrane blebbing.2016
Author(s)
Tadokoro, R., Murai, H., Sakai, K., Okui, T, Yokota, Y., Takahashi, Y.
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Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 38277-38277
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Tale of tails2016
Author(s)
Takahashi, Y.
Organizer
Society for Developmental Biology 75th Annual Meeting International Society of Differentiation 19th International Conference, 【Anne McLaren Memorial Lecture Awad】 (ISD Award Lecture)
Place of Presentation
Boston (米国)
Year and Date
2016-08-05
Related Report
Int'l Joint Research / Invited
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[Presentation] 「しっぽの発生学」2016
Author(s)
高橋淑子
Organizer
京都産業大学セミナー
Place of Presentation
京都産業大学(京都市)
Year and Date
2016-03-01
Related Report
Invited
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[Presentation] Neural Crest and Its Neighbors.2016
Author(s)
Takahashi, Y.
Organizer
“Symposium: Stem Cells, Gene Regulatory Networks and the Evolution of Vertebrates”
Place of Presentation
California Institute of Technology (Los Angels)
Year and Date
2016-01-30
Related Report
Int'l Joint Research / Invited
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[Presentation] 「しっぽの中の神経系」2016
Author(s)
高橋淑子
Organizer
新適塾「脳はおもしろい」第11回会合
Place of Presentation
千里ライフサイエンスセンター(豊中市)
Year and Date
2016-01-07
Related Report
Invited
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