糖鎖生物学と神経科学の融合による神経糖鎖生物学領域の創成

• • 岡 昌吾
  • OKA, Shogo
  • 研究者番号：60233300
  • 京都大学・医学研究科・教授

• ---

• 研究期間

  2004年度〜2008年度

• 研究分野

• 審査区分

• 研究種目

  学術創成研究費

• 研究機関

  京都大学

• 配分額

  • 2004年度：107900千円 (直接経費：83000千円, 間接経費：24900千円)
  • 2005年度：104520千円 (直接経費：80400千円, 間接経費：24120千円)
  • 2006年度：101400千円 (直接経費：78000千円, 間接経費：23400千円)
  • 2007年度：100750千円 (直接経費：77500千円, 間接経費：23250千円)
  • 2008年度：101400千円 (直接経費：78000千円, 間接経費：23400千円)

研究分担者

• • MA, BRUCE
  • 研究者番号：00378788
  • 立命館大学・COE推進機構・准教授
• • 小堤 保則
  • KOZUTSUMI, Yasunori
  • 研究者番号：70205425
  • 京都大学・生命科学研究科・教授
• • 木下 政人
  • KINOSITA, Masato
  • 研究者番号：60263125
  • 京都大学・農学研究科・助教

• ---

• • 川崎 ナナ
  • KAWASAKI, Nana
  • 研究者番号：20186167
  • 国立薬品食品衛生研究所・生物薬品部・室長
• • 石 龍徳
  • SEKI, Tatsunori
  • 研究者番号：20175417
  • 東北大学・医学研究科・准教授

連携研究者

• • 伊藤 信行
  • ITOH, Nobuyuki
  • 研究者番号：10110610
  • 京都大学・薬学研究科・教授

雑誌論文

• D. Anzai, Y. Tonoyama, A. Ikeda, T. Kawasaki and S. Oka: "Regulated expression of HNK-1 carbohydrate is essential for medaka (Oryzias latipes) embryogenesis" Glycobiology (in press). (2009), 1

• Y. Tonoyama, D. Anzai, A. Ikeda, S. Kakuda, M. Kinoshita, T. Kawasaki, S. Oka: "Essential role of beta 1,4-galactosyltransferase 2 during medaka (Oryzias latipes) gastrulation" Mech. Dev (in press). (2009), 1

• T. Yoshihara, K. Sugihara, Y. Kizuka, S. Oka and M. Asano: "Learning/memory impairment and reduced expression of the HNK-1 carbohydrate in beta 4-galactosyltransferase-II-deficient mice" J. Biol. Chem 284 (18). 12550-12561 (2009), 1

• Y. Kizuka, Y. Tonoyama and S. Oka: "Distinct transport and intracellular activities of two GlcAT-P isoforms" J. Biol. Chem 284(14). 9247-9256 (2009), 1,

• N. Kawasaki, CW. Lin CW, R. Inoue, KH. Khoo, N. Kawasaki, BY. Ma, S. Oka, M. Ishiguro, T. Sawada, H. Ishida, T. Hashimoto and T. Kawasaki: "Highly Fucosylated N-Glycan Ligands for Mannan-binding Protein Expressed Specifically on CD26 (DPPVI) Isolated from a Human Colorectal Carcinoma Cell Line" SW1116. Glycobiology 19(4). 437-450 (2009), 1

• E. Shimada, M. Kinoshita and K. Murata: "Expression of cardiac myosin light chain 2 (mcmlc2) during embryonic heart development in medaka fish, Oryzias latipes, and phylogenetic relationship with other myosin light chains" Develop. Growth Differ 51. 1-16 (2009), 1,

• H. Yagi, K. Yamada, E. Ohno, M. Utsumi, Y. Yamaguchi, E. Kurimoto, N. Takahashi N, S. Oka, T. Kawasaki and K. Kato: "Development and application of high performance liquid chromatography map of glucuronyl N-glycans" Open Glycoscience 1. 8-18 (2008), 1,

• I. Morita, Y. Kizuka, S. Kakuda and S. Oka: "Expression and function of the HNK-1 carbohydrate" J. Biochem 43. 719-724 (2008), 1,

• Y. Kizuka, K. Kobayashi, S. Kakuda, Y. Nakajima, S. Itoh, N. Kawasaki and S. Oka: "Laminin-1 is a novel carrier glycoprotein for the non-sulfated HNK-1 epitope in mouse kidney" Glycobiology 18. 331-338 (2008), 1,

• A. Burgess, SR. Wainwright, LS. Shihabuddin, U. Rutishauser, T. Seki and I.. Aubert: "Polysialic acid regulates the clustering, migration, and neuronal differentiation of progenitor cells in the adult hippocampus" Develop Neurobiol 68. 1580-1590 (2008), 1,

• S. Itoh, A. Hachisuka, N. Kawasaki, N. Hashii, R. Teshima, T. Hayakawa, T. Kawanishi and T. Yamaguchi: "Glycosylation analysis of IgLON family proteins in rat brain by liquid chromatography/multiple-stage mass spectrometry" Biochemistry 47. 10132-10154 (2008), 1,

• M. Inoue, K. Kato, H. Matsuhashi, Y. Kizuka, T. Kawasaki and S. Oka: "Distributions of glucuronyltransferases, GlcAT-P and GlcAT-S, and their target substrate, the HNK-1 carbohydrate epitope in the adult mouse brain with or without a targeted deletion of the GlcAT-P gene" Brain Res 1179. 1-15 (2007), 1

• BY. Ma, N. Nakamura, V. Dlabac, H. Naito, S. Yamaguchi, M. Ishikawa, M. Nonaka, M. Ishiguro, N. Kawasaki, S. Oka and T. Kawasaki: "Isolation, cloning, and characterization of a novel phosphomannan-binding lectin from porcine serum" J. Biol. Chem 282 (17). 12963-75 (2007), 1

• BY. Ma, M. Kaihama, M. Nonaka, S. Oka, N. Kawasaki and T. Kawasaki: "LPS suppresses expression of asialoglycoprotein-binding protein through TLR4 in thioglycolate-elicited peritoneal macrophages" Glycoconj. J 24. 243-249 (2007), 1

• T. Seki, T. Namba, H. Mochizuki and M. Onodera: "Clustering, migration and neurite formation of neural precursor cells in the adult rat hippocampus" J Comp Neurol 502. 275-290 (2007), 1,

• H. Yamamoto, H. Takematsu, R. Fujinawa, Y. Naito, Y. Okuno, G. Tsujimoto, A. Suzuki and Y. Kozutsumi: "Correlation Index-Based Responsible-Enzyme Gene Screening (CIRES), a Novel DNA Microarray-Based Method for Enzyme Gene Involved in Glycan Biosynthesis" PLoS ONE 2. e1232 (2007), 1

• Y. Naito, H. Takematsu, S. Koyama, S. Miyake, H. Yamamoto, R. Fujinawa, M. Sugai, Y. Okuno, G. Tsujimoto, T. Yamaji, Y. Hashimoto, S. Itohara, T. Kawasaki, A. Suzuki and Y. Kozutsumi: "Germinal center marker GL7 probes activation-dependent repression of N-glycolylneuraminic acid, a sialic acid species involved in the negative modulation of B-cell activation" Mol. Cell Biol 27. 3008-3022 (2007), 1

• N. Koike, Y. Kassai, Y. Kouta, H. Miwa, M. Konishi and N. Itoh: "Brorin a novel secreted BMP antagonist, promotes neurogenesis in mouse neural precursor cells" J. Biol. Chem 282. 15843-15850 (2007), 1

• T. Shiba, S. Kakuda, M. Ishiguro, I. Morita, S. Oka, T. Kawasaki, S. Wakatsuki and R. Kato: "Crystal structure of GlcAT-S, a human glucuronyltransferase, involved in the biosynthesis of the HNK-1 carbohydrate epitope" Proteins 65 (2). 499-508 (2006), 1

• Y. Kizuka, T. Matsui, H. Takematsu, Y. Kozutsumi, T. Kawasaki and S. Oka: "Physical and functional association of glucuronyltransferases and sulfotransferase glucuronyltransferases and sulfotransferase involved in HNK-1 biosynthesis" J. Biol. Chem. 281(19). 13644-13651 (2006), 1,

• S. Itoh, N. Kawasaki, N. Hashii, A. Harazono, Y. Matsuishi, T. Kawanishi and T. Hayakawa: "N-linked oligosaccharide analysis by liquid chromatography with graphitized carbon column/ liner ion trap-Fourier transform ion cyclotron resonance mass spectrometry in positive and negative ion modes" J. Chromatogr. A 1103. 296-306 (2006), 1

• M. Hirano, BY. Ma, N. Kawasaki, K. Okimura, M. Baba, T. Nakagawa, K. Miwa, N. Kawasaki, S. Oka and T. Kawasaki: "Mannan-binding protein blocks the activation of metalloproteases meprin alpha and beta" J. Immunology 175 (5). 3177-3185 (2005), 1

• H. Tagawa, Y. Kizuka, T. Ikeda, S. Itoh, N. Kawasaki, H. Kurihara, M. L. Onozato, A. Tojo, T. Sakai, T. Kawasaki and S. Oka: "Non-sulfated form of the HNK-1 carbohydrate is expressed in mouse kidney" J. Biol. Chem 280 (25). 23876-23883 (2005), 1,

• M.Terada, K.H. Khoo, R. Inoue, C.I. Chen, K. Yamada, H. Sakaguchi, N. Kadowaki, BY. Ma, S. Oka, T. Kawasaki and N. Kawasaki: "Characterization of oligosaccharide ligands expressed on SW1116 cells recognized by Mannan-binding protein a highly fucosylated polylactosamine type N-glycan" J. Biol. Chem. 280 (12). 10897-10913 (2005), 1

• S. Kakuda, Y. Sato, Y. Tonoyama, S. Oka and T. Kawasaki: "Different acceptor specificities of two glucuronyltransferases involved in the biosynthesis of HNK-1 carbohydrate" Glycobiology 15 (2). 203-210 (2005), 1

• A. Miyake, Y. Nakayama, M. Konishi and N. Itoh: "Fgf19 regulated by Hh signaling is required for zebrafish forebrain development" Dev. Biol 228. 259-275 (2005), 1,

• K. Uemura, H. Yamamoto, T. Nakagawa, K. Nakamura, N. Kawasaki, S. Oka, B.Y. Ma and T. Kawasaki: "Superoxide production from human polymorphonuclear leukocytes by human mannan-binding protein (MBP)" Glycoconj J 21. 79-84 (2004), 1,

• S. Kakuda, T. Shiba, M. Ishiguro, H. Tagawa, S. Oka, Y. Kajiwara, T. Kawasaki, S. Wakatsuki and R. Kato: "Structural basis for acceptor substrate recognition of a human glucuronyltransferase, GlcAT-P, an enzyme critical in the biosynthesis of the carbohydrate epitope HNK-1" J Biol Chem 279. 22693-22703 (2004), 1

• S. Kakuda, S. Oka, T. Kawasaki: "Purification and characterization of two recombinant human glucuronyltransferases involved in the biosynthesis of HNK-1 carbohydrate in Escherichia coli" Protein Expr Purif 35. 111-119 (2004), 1,

学会発表

• Y. Kizuka, Y. Tonoyama, S. Oka: "Two GlcAT-P isoforms are distinctly transported in cell. Clinical and Translational Research on Cancer" Glycomics Applications. (20090326). 伊勢

• D. Anzai, Y. Tonoyama, E. Tane, S. Oka: "The functional analysis of HNK-1 carbohydrate in medaka embryogenesis Joint meeting of the Socie'te' France de Biologie du De'velopment" Japanese Society of Developmental Biologists. (20080913-17). フランスGiens

• 木塚康彦、竹内祐介、森田一平、角田品子、岡昌吾: "糖転移酵素GlcAT-Pの細胞質領域による分泌制御と活性調節" 第28回日本糖質学会. (20080820). つくば

• 岡昌吾: "スパイン形成におけるHNK-1糖鎖の役割" 第31 回日本神経科学学会. (20080710). 東京

• 殿山泰弘、安西大輔、池田篤史、角田品子、木下政人、川崎敏祐、岡昌吾: "Beta1,4-galactosyltransferase 2 is required for convergence and extension movements during medaka (Oryzias latipes) gastrulation" 第41回日本発生生物学会(国際発生生物学会と合同). (20080528). 徳島

• 山下彩貴、木塚康彦、角田品子、岡昌吾: "GlcAT-P遺伝子欠損マウスにおける残存HNK-1糖鎖発現に関する研究" 糖鎖発現に関する研究第128年回日本薬学会. (20080326-28). 横浜

• 岡昌吾: "発生過程に重要な糖鎖の機能解" 第128年回日本薬学会. (20080326-28). 横浜

• 岡昌吾: "神経機能に重要なHNK-1糖鎖BMB2007" 第30回日本分子生物学会年会、第80回日本生化学会大会合同大会. (20071211-15). 横浜

• 岡昌吾: "神経可塑性とHNK-1糖鎖" 第5回糖鎖科学コンソーシアムシンポジウム. (20071126-27). 東京

• Y. Kizuka, N. Kawasaki, N. Maeda, T. Kawasaki and S. Oka: "Characterization of a unique HNK-1 (Human Natural Killer-1) carbohydrate on phosphacan in mouse brain. Glyco19" XIX International Symposium on Glycoconjugates. (20070715-20). Cairns, Australia

• Y. Tonoyama, D. Anzai, A. Ikeda, S. Kakuda, M. Kinoshita, T. Kawasaki, S. Oka: "Essential role of beta1,4-galactosyltransferase 2 in convergence and extension movements during medaka (Oryzias latipes) embryogenesi" HUPO 6th annual world congress. (20071006-10). Seoul, Korea

• 岡昌吾: "神経可塑性におけるHNK-1糖鎖の役割" 日本薬学会第127年会. (20070300). 富山

• 岡昌吾: "HNK-1糖鎖の機能" 第4回糖鎖科学コンソーシアムシンポジウム. (20061023-24). 東京

• 小林恭子, 陣内俊和, 角田品子, 木塚康彦, 高橋智, 川崎敏祐, 岡昌吾: "HNK-1糖鎖生合成酵素GlcAT-S遺伝子欠損マウスの作成とその生化学的解釈" 第26回日本糖質学会年会. (20060823-25). 仙台

• S. Oka, Y. Kizuka, S. Kakuda, I. Morita, S. Yamamoto and T. Kawasaki: "Function and Regulation of the HNK-1 Carbohydrate in the Nervous System" GlycoT 2006 5^<th> International Symposium on Glycosyltransferases. (20060625-28). Tsukuba(Japan)

• I. Morita, T. Shiba, S. Kakuda, S. Oka, T. Kawasaki, S. Watatsuki, R. Kato: "Structural and Biochemical Studies on Human Glucuronyltransferase, GlcAT-S" Involved in the Biosynthesis of the Carbohydrate HNK-1.20^<th> IUBMB Inteanational Congress of Biochemistry and Molecular Biology and 11^<th> FAOBMB Congress. (20060618-23). Kyoto(Japan)

• Y. Kizuka, N. Maeda, N. Kawasaki, T. Kawasaki, S. Oka: "A unique type of HNK-1 carbohydrate expressed on phosphacan is biosynthesized by GlcAT-P" 20^<th> IUBMB Inteanational Congress of Biochemistry and Molecular Biology and 11^<th> FAOBMB Congress. (20060618-23). Kyoto(Japan)

• 殿山泰弘, 安西大輔, 大上志穂, 川崎敏祐, 岡昌吾: "メダカ胚発生に関わる糖転移酵素B4GALT2 の機能解析" 日本発生生物学会第39回大会. (20060531-0603). 広島

• 安西大輔, 殿山泰弘, 大上志穂, 川崎敏祐, 岡昌吾: "メダカを用いたHNK-1糖鎖の機能解析" 日本発生生物学会第39回大会. (20060531-0603). 広島

• 岡昌吾: "メダカの発生における糖鎖の重要性" 第28回日本分子生物学会. (20051200). 福岡

• S. Oka, H. Tagawa, Y. Kizuka, N. Kawasaki, H. Kurihara and T. Kawasaki: "Expression of the non-sulfated HNK-1 carbohydrate in mouse kidney" Pacifichem 2005. (20051200). Honolulu, Hawaii

• Y. Tonoyama, D. Anzai, S. Ogami and S. Oka: "A traial with medaka (oryzias latipes) to analyze the function of glycans involved in the embryogenesis" 2nd Soul-Osaka-Kyoto Joint Symosium on Pharmaceutical Sciences. (20051205-06). Osaka

図書

• Y. Tonoyama and S. Oka: "Cell trace experiment with a caged fluorescent dye during medaka gastrulation in Medaka: Biology, Management, and Experimental Protocols. (M. Kinoshita, K. Murata, K. Naruse, M. Tanaka, Eds)" Wiley-Blackwell. (in press) (2009)

• S. Kakuda, T. Kawasaki and S. Oka: "Glucuronyltransferases Involved in the HNK-1 Biosynthesis. in Experimental Glycoscience-Glycobiology (N. Taniguchi, A. Suzuki, Y. Ito, H. Narimatsu, T. Kawasaki, and S. Hase, Eds)" Springer. pp47-49 (2008),

• Y. Kizuka and S. Oka: "Functional Roles of the HNK-1 Carbohydrate and Polysialic Acid in the Nervous System. in Experimental Glycoscience-Glycobiology (N. Taniguchi, A. Suzuki, Y. Ito, H. Narimatsu, T. Kawasaki, and S. Hase, Eds)" Springer. 180-181 (2008)

• I. Morita and S. Oka: "Glucuronyltransferase (GlcAT-P) Gene-Deficient Mice. In Experimental Glycoscience-Glycobiology (N. Taniguchi, A. Suzuki, Y. Ito, H. Narimatsu, T. Kawasaki, and S. Hase, Eds)" Springer. 381-382 (2008),

• http://www.med.kyoto-u.ac.jp/J/grad_school/introduction/5241/

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