Studies on the biochemical mechanism of N_2O generation by marine nitrifying bacterium
Project/Area Number |
20510007
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Environmental dynamic analysis
|
Research Institution | Shizuoka University |
Principal Investigator |
FUJIWARA Taketomo Shizuoka University, 理学部, 教授 (80209121)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 地球温暖化 / 亜酸化窒素 / 温室効果ガス / 一酸化窒素 / 硝化細菌 / 海洋 |
Research Abstract |
Hydroxylamine oxidoreductase (HAO) was purified from marine ammonia-oxidizing bacterium (AOB) strain NS58. Generation of N_2O as a by-product of HAO reaction was evidenced by in vitro experiment using the purified enzyme. Unique isotopic property of the N_2O generated by HAO reaction was also revealed. Molecular and enzymatic features of nitrite reductase, that was involved in the nitrifier denitrification pathway (another N_2O generation mechanism in AOB), was investigated using the recombinant enzyme.
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Report
(4 results)
Research Products
(36 results)