Identification of polyembryogenesis regulation gene in Citrus and diversification.
Project/Area Number |
21580005
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Breeding science
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Research Institution | Shizuoka University |
Principal Investigator |
OMURA Mitsuo 静岡大学, 農学部, 教授 (90355397)
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 遺伝子 / ゲノム / 育種 / 発生・分化 / 育種学 |
Research Abstract |
To understand the molecular mechanism of apomixis in plant, the positional cloning method was applied to identify the gene regulating nucellar embryogenesis in Citrus. The 380 kb covering the locus responsible to somatic embryogenesis on the linkage group 1 was sequenced and the diversification of the region was surveyed. The ORFs detected in the region were compared in their structure and expression between polyembryony and monoembryony haplotypes. The genes expressing during the somatic embryogenesis from cultured callus were also explored to associate with the responsible gene in nucellar embryogenesis.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Characterization of genomic sequence showing strong association with polyembryony among diverse Citrus species and cultivars, and its synteny with Vitis and Populus2012
Author(s)
Nakano, M., T.Shimada, T.Endo, H.Fujii, H.Nesumi, M.Kita, M.Ebina, T.Shimizu, M.Omura
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Journal Title
Plant Science
Volume: 183
Pages: 131-142
Related Report
Peer Reviewed
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[Journal Article] PCR primer for marker assisted backcrossing to introduce a CTV resistance gene from Poncirus trifoliate(L.) Raf. into Citurs.2011
Author(s)
Ohta, S., T. Endo, T. Shimada, H. Fujii, T. Shimizu, T. Kuniga, T. Yoshioka, H. Nesumi, T. Yoshida, and M. Omura
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Journal Title
J. Japan. Soc. Hort. Sci
Volume: 80
Pages: 295-307
Related Report
Peer Reviewed
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[Journal Article] PCR primer for marker assisted backcrossing to introduce a CTV resistance gene from Poncirus trifoliate (L.) Raf.into Citurs2011
Author(s)
Ohta, S., T.Endo, T.Shimada, H.Fujii, T.Shimizu, T.Kuniga, T.Yoshioka, H.Nesumi, T.Yoshida, M.Omura
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Journal Title
J.Japan.Soc.Hort.Sci.
Volume: 80
Pages: 295-307
Related Report
Peer Reviewed
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[Presentation] Characterization of genomic sequence for the polyembryony locus in Citrus by expression and association analysis2010
Author(s)
Omura, M., M.Nakano, H.Fujii, K.Kigoshi, T.Shimada, T.Endo, T.Shimizu, M.Ebina.
Organizer
The International Conference on the status of Plant & Animal genome research. (PAG-XVIII)
Place of Presentation
San Diego, CA, USA
Related Report
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