KAKEN — Research Projects | Researcher Number: 30072888

1. Analysis of the species specific pathogenicity of morbillivirus by reverse genetics.

Research Project

Research Category	Grant-in-Aid for Scientific Research (A)
Research Field	Basic veterinary science/Basic zootechnical science
Research Institution	The University of Tokyo
Principal Investigator	KAI Chieko The University of Tokyo, Institute of Medical Science, Professor
Project Period (FY)	2000 – 2003Completed
Keywords	mononega virus / new reverse genetics / rinderpest virus / canine distemper virus
Research Abstract	Emerging and reemerging virus infection by the mono-nega virus pose a social problem globally, and the elucidation of the infection mechanism exceedin ...

Research Products of this project	Publications (145 results)

2. Molecular mechanisms of pathogenicity of rinderpest virus

Research Project

Research Category	Grant-in-Aid for Scientific Research (A).
Research Field	Basic veterinary science/Basic zootechnical science
Research Institution	Nippon Institute for Biological Science
Principal Investigator	YAMANOUCHI Kazuya Nippon Inst. Biol. Sci., director
Project Period (FY)	1998 – 2000Completed
Keywords	Rinderpest / Pathogenicity / Reverse genetics / Immune mechanism / Recombinant vaccine
Research Abstract	System to recover infectious rinderpest virus (RPV) from cDNA copy of the virus genome was established on the RBOK strain of RPV, and role of virus ge ...

Research Products of this project	Publications (26 results)

3. Development of Analysis Method of Immune Surveillance System in Various Animals by using Adenovirus Vector.

Research Project

Research Category	Grant-in-Aid for Scientific Research (B).
Research Field	Applied veterinary science
Research Institution	The University of Tokyo
Principal Investigator	KAI Chieko LABORATORY ANIMAL RESEARCH CENTER, INSTITUTE OF MEDICAL SCIENCE, THE UNIVERSITY of TOKYO
Project Period (FY)	1997 – 2000Completed
Keywords	adenovirus / canine distemper / CDV / H protein / CTL
Research Abstract	The AIM of this research is the development of analysis method of immune surveillance system in various animals by using adenovirus vector to characte ...

Research Products of this project	Publications (165 results)

4. Molecular pathogenesis of transmissible spongiform encephalopathy

Research Project

Research Category	Grant-in-Aid for Scientific Research (A)
Research Institution	Nippon Institute for Biological Science
Principal Investigator	YAMANOUCHI Kazuya Nippon Inst. Biol. Sci., Director
Project Period (FY)	1997 – 1998Completed
Keywords	prion / scrapie / transmissible spongiform encephalopath / neurological diseases / prion diseases / Creutzfeldt-Jakob disease
Research Abstract	Molecular pathogenesis of prion disease was studied based on the analysis of prion protein (PrP) gene.Several new findings were also obtained from the ...

Research Products of this project	Publications (28 results)

5. Molecular mechanisms of pathogenicity of rinderpestvirus and its molecular epidemio1ogy

Research Project

Research Category	Grant-in-Aid for international Scientific Research
Research Field	Basic veterinary science/Basic zootechnical science
Research Institution	Nippon Institute for Biological Science
Principal Investigator	YAMANOUCHI Kazuya Nippon Inst Bio1 Sc1, Director
Project Period (FY)	1996 – 1997Completed
Keywords	Rinderpest virus / Recombinant vaccine / Infection Immunity / Cell-mediated immunity / H protein / NP protein
Research Abstract	1. Studies on the efficacy of recombinant rinderpest vaccine

Research Products of this project	Publications (23 results)

6. Studies on molecular basis of pathogenesis of rinderpest virus and molecular epidemiology

Research Project

Research Category	Grant-in-Aid for international Scientific Research
Research Institution	Nippon Institute for Biological Science
Principal Investigator	YAMANOUCHI Kazuya Nippon Institute for Biological Science, Chief Researcher
Project Period (FY)	1995Completed
Keywords	Rinderpest virus / Pathogenesis / Molecular epidemiology / Recombinant vaccine / Genetic analysis / PPR virus / Molecular biology / Celluar immunity
Research Abstract	The aim of the collaborative research on rinderpest virus was to conduct basic virological and immunological studies and to apply the results on the m ...

Research Products of this project	Publications (10 results)

7. Development of cytotoxicity test for outbred animals using recombinant viruses.

Research Project

Research Category	Grant-in-Aid for Developmental Scientific Research (B)
Research Field	Applied veterinary science
Research Institution	The University of Tokyo
Principal Investigator	KAI Chieko Univ.of Tokyo, Fac.of Agr.Associate Professor
Project Period (FY)	1993 – 1995Completed
Keywords	CTL / cell mediated cytotoxic assay / adenovirus vector / rinderpest virus / measles virus / bovine leukemia virus / recombinant / domestic animals
Research Abstract	The cytotoxic T lymphocytes (CTL) have been considered to play an important role in immunity against the infections of many kinds of viruses. However, ...

Research Products of this project	Publications (36 results)

8. 牛疫ウイルスによって誘発される免疫不全機序の研究

Research Project

Research Category	Grant-in-Aid for General Scientific Research (C)
Research Field	基礎獣医学
Research Institution	The University of Tokyo
Principal Investigator	甲斐知恵子東京大学, 農学部, 助教授
Project Period (FY)	1992Completed
Keywords	牛疫ウイルス / ウサギ / 近交系 / 免疫抑制 / T細胞 / 幼若化反応 / PHA
Research Abstract	本研究は、免疫異常を誘発する牛疫ウイルスのウサギ感染モデル系を用い、ウイルス誘発性免疫不全の機序を解明することを目的としている。近年開発された近交系ウサギと病原性を保持しているB95a細胞継代牛疫ウイルスにより、ウイルス感染後のウイルス増殖やリンパ系組織に及ぼす影響、さらに免疫抑制機序に細胞性因子が ...

9. 亜急性硬化性全脳炎(SSPE)ウイルスの持続感染機構

Research Project

Research Category	Grant-in-Aid for General Scientific Research (C)
Research Field	Virology
Research Institution	The University of Tokyo
Principal Investigator	山内一也東京大学, 医科学研究所, 教授
Project Period (FY)	1991Completed
Keywords	SSPEウイルス / 麻疹ウイルス / 持続感染 / 脳炎
Research Abstract	SSPEウイルスの持続感染モデルとして欠損性ウイルス産生を特徴とするYamagataー1株感染Vero細胞についてウイルスクロ-ンの分離を試みた。その結果、遊離ウイルスの産生が見られないYAクロ-ンと遊離ウイルスの産生が見られるYFクロ-ンの2種が得られた。これら2クロ-ンについて単クロ-ン抗体によ ...

Research Products of this project	Publications (4 results)

10. 初期発生における割球分化の分子機構

Research Project

Research Category	Grant-in-Aid for Scientific Research on Priority Areas
Research Institution	Kyoto University
Principal Investigator	入谷明京都大学, 農学部, 教授
Project Period (FY)	1991Completed
Keywords	始原生植細胞 / 胚奇形腫 / 遺伝子導入動物 / 体外受精 / 癌遺伝子 / 核移植 / 胚培養 / 相同的組替え
Research Abstract	生殖系列の増殖と分化の機構が分子から細胞に焦点を充てて、初期胚の発生過程を通して分析され、それらによって得られた結果を利用して医用動物や家蓄生産に応用した。得られた主な成果は(1)実験小動物、サルおよび家蓄を含めた哺乳動物胚の体外受精、培養系や胚操作技術を確立した。(2)始原生殖細胞や性腺細胞を含む ...

Research Products of this project	Publications (6 results)

11. Causes of high in cidence of SSPE in Pak and JE-like disease

Research Project

Research Category	Grant-in-Aid for international Scientific Research
Research Institution	Nihon University
Principal Investigator	TAKASU Toshiaki Nihon Univ. Sch. Med., Prof. Neurol.
Project Period (FY)	1991 – 1993Completed
Keywords	Pakistan / Subacute sclerosing panencephalitis / Japanese encephalitis virus / West Nile virus / Measles / Measles vaccine / PCR / Acute encephalitis
Research Abstract	An elevated SSPE occurrence persisted during 1991-93 in Karachi, Pakistan ; 36 new cases were registered at Civil Hospital Karachi in that period (1.3 ...

Research Products of this project	Publications (86 results)

12. The immune functions in aged cynomolgus monkeys as an experimental model.

Research Project

Research Category	Grant-in-Aid for General Scientific Research (C)
Research Field	Laboratory animal science
Research Institution	The university of Tokyo
Principal Investigator	KAI Chieko Univ. of Tokyo, Fac. of Agr., Associate Professor
Project Period (FY)	1990 – 1991Completed
Keywords	aging / cynomolgus monkey / immune function / T lymphocyte / B lymphocyte / NL cell / macrophage
Research Abstract	According to the increase in population of aged people, the immune status of aged humans is demanded to be studied to prevent them from disease's. We ...

Research Products of this project	Publications (24 results)

13. 初期発生における割球分化の分子機構

Research Project

Research Category	Grant-in-Aid for Scientific Research on Priority Areas
Research Institution	Kyoto University
Principal Investigator	入谷明京都大学, 農学部, 教授
Project Period (FY)	1990Completed
Keywords	哺乳動物初期胚 / 形質転換動物 / キメラ / テラト-マ / サル / ウシ / マウス / 分化遺伝子
Research Abstract	サルの過剰排卵卵子の媒精7日後には胚盤胞期胚にまで発育する胚が得られた。マウス卵管の培養上清の分子量5、000以下の分画に初期分割胚の発育因子の存在することが認められた。ウシ体外受精由来8細胞期胚の単離割球と除核卵母細胞との再構成胚の40%は8細胞期に発育した。注入キメラ法によってマウスの正常胚盤胞 ...

Research Products of this project	Publications (16 results)

14. 初期発生の分子機構

Research Project

Research Category	Grant-in-Aid for Scientific Research on Priority Areas
Research Institution	Kyoto University
Principal Investigator	入谷明京都大学, 農学部, 教授
Project Period (FY)	1989Completed
Keywords	哺乳動物初期胚 / 体外受精 / 共培養 / 再構成卵巣 / 癌遺伝子 / 形質転換動物 / 成長ホルモン遺伝子 / インターフェロン遺伝子
Research Abstract	哺乳動物初期胚の培養法と発生調節法を確立すると共に、胚発生の分化を遺伝子レベルで解析した。サル精子の受精能獲得には果糖が必要とされ、受精後2細胞期までは血清非添加で、それ以降は5%血清添加培地で良好な発生率が得られた。ウシでは顆粒膜細胞や卵管上皮細胞との共培養系で維持された体外受精卵の産子への発生を ...

Research Products of this project	Publications (12 results)

15. 初期発生の分子機構

Research Project

Research Category	Grant-in-Aid for Scientific Research on Priority Areas
Research Institution	Kyoto University
Principal Investigator	入谷明京都大学, 農学部, 教授
Project Period (FY)	1988Completed
Keywords	カニクイザル / 体外培養 / 胚割球の分離 / 胚の分化と増殖 / 再構成卵巣 / 癌遺伝子 / F9細胞 / グロビン / チミヂンキナーゼ遺伝子
Research Abstract	生殖系列の維持機構そのものを配偶子の分化、受精着床および初期発生に分けて基礎生物学的な機構の解明を個体、器官、細胞及び分子の各レベルで解明を進めながら、その成果を体外受精、胚操作および胚移植などの生殖諸相の人為調節に結び付けるのが目的である。哺乳動物胚の体外培養操作法を確立するために、カニクイザルと ...

Research Products of this project	Publications (6 results)

16. A factor analysis for the elevated incidence of subacute sclerosing panencephalitis and an epidemiological study of the Japanese encephalitis-like disease in Pakistan

Research Project

Research Category	Grant-in-Aid for international Scientific Research
Research Institution	Nihon University
Principal Investigator	TAKASU Toshiaki Nihon University School of Medicine, Dept. of Neurology
Project Period (FY)	1988 – 1990Completed
Keywords	Subacute sclerosing panencephalitis / age at occurrence of measles / case control study / vaccination / HLA analysis / hemaglutinin (HA) protein / Japanese encephalitis
Research Abstract	We reported formerly that in Karachi the incidence of subacute sclerosing panencephalitis (SSPE) could be more than fifty times higher than in Japan a ...

Research Products of this project	Publications (39 results)

17. Molecular genetics of subactute sclerosing panencephalitis (SSPE) virus

Research Project

Research Category	Grant-in-Aid for General Scientific Research (B)
Research Field	Virology
Research Institution	The University of Tokyo
Principal Investigator	YAMANOUCHI Kazuya Inst. Med. Sci., Univ. Tokyo, Professor
Project Period (FY)	1987 – 1989Completed
Keywords	SSPE virus / Measles virus / Molecular genetics / Neurovirulence / Persistent infection / Neurotoxin / Pathogenesis / Slow virus infection
Research Abstract	(1) Gene analysis of SSPE virus, Yamagata-1 strain, was conducted. The entire base sequences of M, NP, H, and F genes as well as part of the sequences ...

Research Products of this project	Publications (33 results)

18. FEVER AS A SYMPTOM IN DEFENSE RESPONSE TO STRESSES.

Research Project

Research Category	Grant-in-Aid for Co-operative Research (A)
Research Field	環境生理学(含体力医学･栄養生理学)
Research Institution	Yamanashi Medical College
Principal Investigator	IRIKI Masami Dept. of Physiology, Yamanashi Medical College
Project Period (FY)	1986 – 1988Completed
Keywords	fever / fever syndrome / defence responses / temperature regulation / interleukin / prostaglandins / tumor necrosis factor
Research Abstract	Fever is not merely the rise of body temperature and relevant abnormality of the body temperature requlation, but is a symptoms of integrated defense ...

Research Products of this project	Publications (28 results)

19. 発癌遺伝子を導入されたトランスジエニック動物での遺伝子産物の発現とその免疫応答

Research Project

Research Category	Grant-in-Aid for Cancer Research
Research Institution	The University of Tokyo
Principal Investigator	森庸厚東大, 医科学研究所, 助教授
Project Period (FY)	1986Completed
Keywords	発癌遺伝子 / トランスジェニック動物 / 免疫応答
Research Abstract	本年度は、トランスジェニック動物作成上の技術的難関であった遺伝子の受精卵前核への移入と仮親への卵管果を通じてのEmbryo-trasrsfer(E・T)のテクニックを習熟することによって、myc遺伝子を導入したマウス産児を実際に得られるようになった。具体的には、PSV2CT myc clone 10 ...

Research Products of this project	Publications (8 results)

20. がん研究のための実験動物の維持と開発

Research Project

Research Category	Grant-in-Aid for Cancer Research
Research Institution	National Institute of Genetics
Principal Investigator	森脇和郎遺伝研, その他, 教授
Project Period (FY)	1986Completed
Keywords	基準系統 / コンジェニック系統 / マウス / ラット / 遺伝的モニタリング / 微生物的モニタリング / ヌードマウス / 受精卵凍結保存 / モルモット / 実験動物リスト / 突然変異系統
Research Abstract	癌研究者に株分与を行うため19系統のマウス基準系統を指定維持し、40施設に26系統合計371匹のマウスを分譲した。これらの基準系統については定期的に遺伝的モニタリングを行い、基準系統としての維持体制を確立した。また7施設から延46系統のマウスについて遺伝的モニタリングの依頼を受けた。近交系ラットにつ ...

Research Products of this project	Publications (8 results)

Project Period (Start Year [FY])

1. Analysis of the species specific pathogenicity of morbillivirus by reverse genetics. Research Project

KAI Chieko The University of Tokyo, Institute of Medical Science, Professor

2. Molecular mechanisms of pathogenicity of rinderpest virus Research Project

YAMANOUCHI Kazuya Nippon Inst. Biol. Sci., director

3. Development of Analysis Method of Immune Surveillance System in Various Animals by using Adenovirus Vector. Research Project

KAI Chieko LABORATORY ANIMAL RESEARCH CENTER, INSTITUTE OF MEDICAL SCIENCE, THE UNIVERSITY of TOKYO

4. Molecular pathogenesis of transmissible spongiform encephalopathy Research Project

YAMANOUCHI Kazuya Nippon Inst. Biol. Sci., Director

5. Molecular mechanisms of pathogenicity of rinderpestvirus and its molecular epidemio1ogy Research Project

YAMANOUCHI Kazuya Nippon Inst Bio1 Sc1, Director

6. Studies on molecular basis of pathogenesis of rinderpest virus and molecular epidemiology Research Project

YAMANOUCHI Kazuya Nippon Institute for Biological Science, Chief Researcher

7. Development of cytotoxicity test for outbred animals using recombinant viruses. Research Project

KAI Chieko Univ.of Tokyo, Fac.of Agr.Associate Professor

8. 牛疫ウイルスによって誘発される免疫不全機序の研究 Research Project

甲斐 知恵子 東京大学, 農学部, 助教授

9. 亜急性硬化性全脳炎(SSPE)ウイルスの持続感染機構 Research Project

山内 一也 東京大学, 医科学研究所, 教授

10. 初期発生における割球分化の分子機構 Research Project

入谷 明 京都大学, 農学部, 教授

11. Causes of high in cidence of SSPE in Pak and JE-like disease Research Project

TAKASU Toshiaki Nihon Univ. Sch. Med., Prof. Neurol.

12. The immune functions in aged cynomolgus monkeys as an experimental model. Research Project

KAI Chieko Univ. of Tokyo, Fac. of Agr., Associate Professor

13. 初期発生における割球分化の分子機構 Research Project

入谷 明 京都大学, 農学部, 教授

14. 初期発生の分子機構 Research Project

入谷 明 京都大学, 農学部, 教授

15. 初期発生の分子機構 Research Project

入谷 明 京都大学, 農学部, 教授

16. A factor analysis for the elevated incidence of subacute sclerosing panencephalitis and an epidemiological study of the Japanese encephalitis-like disease in Pakistan Research Project

TAKASU Toshiaki Nihon University School of Medicine, Dept. of Neurology

17. Molecular genetics of subactute sclerosing panencephalitis (SSPE) virus Research Project

YAMANOUCHI Kazuya Inst. Med. Sci., Univ. Tokyo, Professor

18. FEVER AS A SYMPTOM IN DEFENSE RESPONSE TO STRESSES. Research Project

IRIKI Masami Dept. of Physiology, Yamanashi Medical College

19. 発癌遺伝子を導入されたトランスジエニック動物での遺伝子産物の発現とその免疫応答 Research Project

森 庸厚 東大, 医科学研究所, 助教授

20. がん研究のための実験動物の維持と開発 Research Project

森脇 和郎 遺伝研, その他, 教授

1. Analysis of the species specific pathogenicity of morbillivirus by reverse genetics.

Research Project

2. Molecular mechanisms of pathogenicity of rinderpest virus

Research Project

3. Development of Analysis Method of Immune Surveillance System in Various Animals by using Adenovirus Vector.

Research Project

4. Molecular pathogenesis of transmissible spongiform encephalopathy

Research Project

5. Molecular mechanisms of pathogenicity of rinderpestvirus and its molecular epidemio1ogy

Research Project

6. Studies on molecular basis of pathogenesis of rinderpest virus and molecular epidemiology

Research Project

7. Development of cytotoxicity test for outbred animals using recombinant viruses.

Research Project

8. 牛疫ウイルスによって誘発される免疫不全機序の研究

Research Project

甲斐知恵子東京大学, 農学部, 助教授

9. 亜急性硬化性全脳炎(SSPE)ウイルスの持続感染機構

Research Project

山内一也東京大学, 医科学研究所, 教授

10. 初期発生における割球分化の分子機構

Research Project

入谷明京都大学, 農学部, 教授

11. Causes of high in cidence of SSPE in Pak and JE-like disease

Research Project

12. The immune functions in aged cynomolgus monkeys as an experimental model.

Research Project

13. 初期発生における割球分化の分子機構

Research Project

入谷明京都大学, 農学部, 教授

14. 初期発生の分子機構

Research Project

入谷明京都大学, 農学部, 教授

15. 初期発生の分子機構

Research Project

入谷明京都大学, 農学部, 教授

16. A factor analysis for the elevated incidence of subacute sclerosing panencephalitis and an epidemiological study of the Japanese encephalitis-like disease in Pakistan

Research Project

17. Molecular genetics of subactute sclerosing panencephalitis (SSPE) virus

Research Project

18. FEVER AS A SYMPTOM IN DEFENSE RESPONSE TO STRESSES.

Research Project

19. 発癌遺伝子を導入されたトランスジエニック動物での遺伝子産物の発現とその免疫応答

Research Project

森庸厚東大, 医科学研究所, 助教授

20. がん研究のための実験動物の維持と開発

Research Project

森脇和郎遺伝研, その他, 教授