KAKEN — Research Projects | Researcher Number: 40175485

Search Results: 71 results / Researcher Number: 40175485

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1. Analysis of stemness, aging and carcinogenesis using hematopoietic stem cell ex vivo amplification system

Research Project

Research Category	Grant-in-Aid for Scientific Research (S)
Review Section	Broad Section I
Research Institution	Tokyo Medical and Dental University (2022-2024) The University of Tokyo (2020-2021)
Principal Investigator	中内啓光東京医科歯科大学, 高等研究院, 特別栄誉教授
Project Period (FY)	2020-08-31 – 2025-03-31Granted
Keywords	造血幹細胞 / 加齢 / 発癌 / 体外増幅 / ex vivo増幅 / クローナル造血 / CRISPRgRNAスクリーニング
Outline of Research at the Start	ごく最近、我々はマウス造血幹細胞をin vitroで長期培養して幹細胞としての機能を維持したまま４週間で900倍以上に増殖させる手法を開発し報告した（Wilkinson et al. Nature, 2019)。
Outline of Annual Research Achievements	マウス造血幹細胞長期培養後において造血幹細胞機能と相関する細胞表面マーカーを評価しこれを同定した。また、培養条件の最適化によって造血幹細胞分画のさらなる増殖が得られた。新たに同定した培養後造血幹細胞分画を用いてRNAシークエンスを用いた網羅的遺伝子発現解析を開始しており、現在解析を進めている。
Current Status of Research Progress	2: Research has progressed on the whole more than it was originally planned.
Interim Assessment Comments (Rating)	A: In light of the aim of introducing the research area into the research categories, the expected progress has been made in research.

Research Products of this project	Journal Article (4 results, of which Int'l Joint Research: 4 results, Peer Reviewed: 4 results, Open Access: 4 results) Presentation (4 results, of which Int'l Joint Research: 1 results, Invited: 4 results)

2. マウス造血幹細胞体外増幅系を用いた幹細胞性・加齢・発癌機構の解析

Research Project

Research Category	Grant-in-Aid for Scientific Research (A)
Review Section	Medium-sized Section 54:Internal medicine of the bio-information integration and related fields
Research Institution	The University of Tokyo
Principal Investigator	中内啓光東京大学, 医科学研究所, 特任教授
Project Period (FY)	2020-04-01 – 2021-03-31Discontinued
Keywords	造血幹細胞 / 体外増幅 / 加齢 / クローン性造血 / 発癌 / 遺伝子ノックアウトスクリーニング / マルチオミックス解析
Outline of Research at the Start	ごく最近、我々はマウス造血幹細胞をin vitroで長期培養して幹細胞としての機能を維持したまま４週間で900倍以上に増殖させる手法を開発し報告した（Wilkinson et al. Nature, 2019).。
Outline of Annual Research Achievements	成体マウスより採取したマウス造血幹細胞を体外増幅し、表面抗原に対する抗体パネルにより多重染色を行い、イムノフェノタイプ別に体外増幅培養を行い、同種造血幹細胞移植を実施し、幹細胞および各種前駆細胞の機能的分画を評価した。現在、これらのサンプルのRNA-seqを実施中である。

Research Products of this project	Journal Article (5 results, of which Int'l Joint Research: 5 results, Peer Reviewed: 5 results, Open Access: 1 results)

3. Hematopoietic stem cell control by amino acids and development of novel therapeutic methods

Research Project

Research Category	Grant-in-Aid for Scientific Research (A)
Research Field	Hematology
Research Institution	The University of Tokyo
Principal Investigator	Hiromitsu Nakauchi 東京大学, 医科学研究所, 特任教授
Project Period (FY)	2016-04-01 – 2019-03-31Completed
Keywords	造血幹細胞 / 骨髄ニッチ / アミノ酸 / バリン / BCAA / 骨髄移植 / アミノ酸バランス / 骨髄微小環境 / 骨髄環境 / 造血幹細胞移植学 / 培地
Outline of Final Research Achievements	A specialized bone marrow microenvironment (niche) regulates hematopoietic stem cell (HSC) self-renewal and commitment. For successful donor-HSC engra ...

Research Products of this project	Journal Article (25 results, of which Int'l Joint Research: 25 results, Peer Reviewed: 25 results, Open Access: 8 results, Acknowledgement Compliant: 7 results) Presentation (15 results, of which Int'l Joint Research: 9 results, Invited: 15 results) Patent(Industrial Property Rights) (6 results, of which Overseas: 3 results)

4. Establishment of a specific differentiation induction method to the kidneys constituent cells using a cell memory of the human kidney-derived iPS cells

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Kidney internal medicine
Research Institution	The University of Tokyo
Principal Investigator	TAKASE OSAMU 東京大学, 医学部附属病院, 講師
Project Period (FY)	2013-04-01 – 2016-03-31Completed
Keywords	Epigenetic Memory / 腎臓由来iPS細胞 / 腎系統特異的分化誘導法 / 包括的メチル化解析 / 網羅的マイクロアレイ解析 / 新規腎臓発生・再生因子 / Epigenetics / DNAメチル化解析 / 腎臓細胞記憶 / HDAC阻害剤 / エピジェネティックス / iPS細胞移植 / 腎臓再生 / 包括的DNAメチル化解析
Outline of Final Research Achievements	In this study, we have investigated the difference between fibroblast-derived iPS cells and the kidney-derived iPS cells by two kind renal lineage-spe ...

Research Products of this project	Journal Article (5 results, of which Peer Reviewed: 4 results, Open Access: 4 results) Presentation (23 results, of which Int'l Joint Research: 2 results, Invited: 2 results)

5. Identification of human bone marrow niche to support HSCs

Research Project

Research Category	Grant-in-Aid for Scientific Research (A)
Research Field	Hematology
Research Institution	The University of Tokyo
Principal Investigator	NAKAUCHI Hiromitsu 東京大学, 医科学研究所, 教授
Project Period (FY)	2012-04-01 – 2015-03-31Completed
Keywords	骨髄ニッチ / TGF-beta / 休眠状態
Outline of Final Research Achievements	This project describes identification of human bone marrow niche to support HSCs. We hypothesized that identification of BM niche leads us to manipula ...

Research Products of this project	Journal Article (50 results, of which Peer Reviewed: 50 results, Open Access: 9 results) Presentation (20 results, of which Invited: 20 results) Book (2 results) Patent(Industrial Property Rights) (1 results)

6. Artifical kidney established by human kidney derived iPS cells

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Kidney internal medicine
Research Institution	The University of Tokyo
Principal Investigator	HISHIKAWA Keiichi 東京大学, 医学部附属病院, 准教授
Project Period (FY)	2011-04-28 – 2015-03-31Completed
Keywords	幹細胞 / iPS cells / メチル化 / 再生医学 / iPS細胞 / 再生医療 / マイクロアレイ
Outline of Final Research Achievements	In this study, we firstly identified key genes that determine memory of kidney derived iPS cells. By using these genes, we induced kidney cells effic ...

Research Products of this project	Journal Article (6 results, of which Peer Reviewed: 6 results, Open Access: 2 results) Presentation (7 results) Book (1 results)

7. Development of high-speed closed system for analysis and isolation of rare cell populations within the blood

Planned Research

Research Category	Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Review Section	Science and Engineering
Research Institution	The University of Tokyo
Principal Investigator	nakauchi hiromitsu 東京大学, 医科学研究所, 教授
Project Period (FY)	2011-04-01 – 2016-03-31Completed
Keywords	微小細胞 / デバイス / がん / 閉鎖系 / 医工学融合 / 閉鎖系高速細胞解析分離装置 / 末梢循環がん細胞 / 赤芽球 / 血小板 / ガン
Outline of Final Research Achievements	Aiming to develop high-speed closed system for analysis and isolation of rare cell populations within the blood, in this study, we showed the possibil ...
Project Area	Hyper Bio Assembler for 3D Celluler Innovation

Research Products of this project	Journal Article (90 results, of which Int'l Joint Research: 4 results, Peer Reviewed: 83 results, Open Access: 11 results) Presentation (45 results, of which Int'l Joint Research: 14 results, Invited: 32 results) Book (2 results) Patent(Industrial Property Rights) (3 results, of which Overseas: 1 results)

8. Research Management

Administrative Group

Research Category	Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
Review Section	Science and Engineering
Research Institution	Osaka University
Principal Investigator	Arai Tatsuo 大阪大学, 基礎工学研究科, 教授
Project Period (FY)	2011-04-01 – 2016-03-31Completed
Keywords	マイクロロボティクス / バイオアセンブラ / マイクロナノロボティクス / メカトロニクス / 再生医療 / 幹細胞生物学 / マイクロナノロボテックス
Outline of Final Research Achievements	We coordinated research groups closely in the area and proceeded fusional research, and presented our research activities in wide area of the domestic ...
Project Area	Hyper Bio Assembler for 3D Celluler Innovation

Research Products of this project	Book (1 results) Remarks (5 results)

9. Development of novel immunotherapy by using of human iPS cells of an antigen-specific T-cell origin

Research Project

Research Category	Grant-in-Aid for Challenging Exploratory Research
Research Field	General medical chemistry
Research Institution	The University of Tokyo
Principal Investigator	NAKAUCI Hiromitsu 東京大学, 医科学研究所, 教授
Project Period (FY)	2010 – 2011Completed
Keywords	抗原特異的T細胞 / iPS細胞 / 幹細胞 / T細胞の分化誘導
Research Abstract	Adoptive immunotherapy with antigen-specific T cells expressing a limited TCR repertoire is a promising approach to fighting various types of cancer a ...

Research Products of this project	Journal Article (17 results, of which Peer Reviewed: 17 results) Presentation (12 results) Patent(Industrial Property Rights) (6 results, of which Overseas: 6 results)

10. Elucidation of the mechanisms underlying maintenance of hematopoietic stem cell hibernation

Research Project

Research Category	Grant-in-Aid for Scientific Research (A)
Research Field	Hematology
Research Institution	The University of Tokyo
Principal Investigator	NAKAUCHI Hiromitsu 東京大学, 医科学研究所, 教授
Project Period (FY)	2009 – 2011Completed
Keywords	造血幹細胞 / CD34陽性細胞 / 骨髄微小環境 / 非髄鞘シュワン細胞 / GFAP陽性細胞 / 白血病幹細胞 / 冬眠 / 骨髄ニッチ / FOXO転写因子 / 脂質ラフト / TGFβ / 冬眠機構 / smad signaling
Research Abstract	Hematopoietic stem cells(HSCs) reside in a bone marrow niche in a nondividing state, from which they occasionally arise to undergo cell division. Yet, ...

Research Products of this project	Journal Article (61 results, of which Peer Reviewed: 61 results) Presentation (22 results) Patent(Industrial Property Rights) (4 results, of which Overseas: 4 results)

11. 発生工学的手法を用いた固型臓器再生の試み

Research Project

Research Category	Grant-in-Aid for Exploratory Research
Research Field	General medical chemistry
Research Institution	The University of Tokyo
Principal Investigator	中内啓光 The University of Tokyo
Project Period (FY)	2007 – 2008Completed
Keywords	固型臓器再生 / ES細胞 / 膵臓 / 臓器再生 / blastcyst complementation / ES cells / 賢臓 / Sall 1
Research Abstract	ホルモン投与により過排卵誘発を施した雌のpdx-1+/マウスを同雄マウスと交配させ、3.5日目に子宮灌流法により胚盤胞を採取する。得られた胚盤胞にβ-アクチンプローモークー下でGFPを発現するドナーES細胞をピエゾーマイクロマニピュレークーを用い10〜12個注入後、精管結紫マウスと交尾後2.5日後の ...

Research Products of this project	Journal Article (5 results, of which Peer Reviewed: 5 results)

12. Identification of niche signal prescribing hibernation of hematopoietic stem cell

Research Project

Research Category	Grant-in-Aid for Scientific Research (A)
Research Field	Hematology
Research Institution	The University of Tokyo
Principal Investigator	NAKAUCHI Hiromitsu The University of Tokyo
Project Period (FY)	2007 – 2008Completed
Keywords	造血幹細胞 / リッピドラフト / 冬眠 / ニッチ / FOXO / TGF-β / ニッチシグナル / リピッドラフト
Research Abstract	(200字程度、難解な専門用語は避けること)骨髄ニッチに存在する造血幹細胞にはLipid raft clustering(LRC)がなく、骨髄中の前駆細胞や骨髄から取り出してきてサイトカイン刺激した造血幹細胞にはLRC が存在するという事実から骨髄ニッチにはLRC 形成を阻害する因子(シグナル)があ ...

Research Products of this project	Journal Article (57 results, of which Peer Reviewed: 25 results) Presentation (25 results) Patent(Industrial Property Rights) (7 results)

13. 新しいIL-6/12関連サイトカインIL-27による造血幹細胞の増殖・分化の制御

Research Project

Research Category	Grant-in-Aid for Exploratory Research
Research Field	Hematology
Research Institution	Tokyo Medical University
Principal Investigator	善本隆之 Tokyo Medical University
Project Period (FY)	2006 – 2008Completed
Keywords	IL-27 / 造血幹細胞 / 増殖と分化 / トランスジェニックマウス / 増殖 / 分化
Research Abstract	IL-27は、最近同定されたIL-6サイトカインファミリーに属するサイトカインの1つで、STAT1とSTAT3の両方を効率良く活性化し、炎症を誘導する作用と炎症を抑制する両作用を有している。IL-27は、そのレセプターであるgp130とWSX-1を介して、T細胞、B細胞やマクロファージなどの多くの細 ...

Research Products of this project	Journal Article (12 results, of which Peer Reviewed: 6 results) Presentation (1 results)

14. Molecular basis of asymmetric division and lineage commitment in hematopoietic stem cells

Research Project

Research Category	Grant-in-Aid for Scientific Research (A)
Research Field	Hematology
Research Institution	The University of Tokyo
Principal Investigator	NAKAUCHI Hiromitsu The University of Tokyo, Institute of Medical Science, Professor
Project Period (FY)	2005 – 2006Completed
Keywords	hematopoietic stem cells / asymmetric division / DNA methylation / self-renewal / commitment
Research Abstract	Stem cells are defined as cells with multilineage differentiation and self-renewal potentials. These cells play a crucial role in the development, reg ...

Research Products of this project	Journal Article (65 results) Book (3 results) Patent(Industrial Property Rights) (4 results)

15. レトロウイルスベクターを用いた養子免疫遺伝子細胞療法の確立

Research Project

Research Category	Grant-in-Aid for Scientific Research on Priority Areas
Review Section	Biological Sciences
Research Institution	University of Tsukuba
Principal Investigator	小野寺雅史筑波大学, 臨床医学系, 講師
Project Period (FY)	2003Completed
Keywords	遺伝子治療 / ウイルス / 癌 / 免疫学 / トランスレーショナルリサーチ
Research Abstract	レトロウイルスベクターを用いて癌関連遺伝子を導入した造血前駆細胞を、サイトカインにて樹状細胞(DC)へと分化・誘導し、これら遺伝子導入DCを用いた効率の良い腫瘍特異的細胞傷害性T細胞(CTL)の誘導法を確立する。[方法・結果]レトロウイルスベクターGCDNsamI/Eにてovalbumin(OVA) ...

Research Products of this project	Publications (6 results)

16. 胚性幹(ES)細胞の分化制御による再生医療に関する基礎的研究

Research Project

Research Category	Grant-in-Aid for Exploratory Research
Research Field	General surgery
Research Institution	Yokohama City University
Principal Investigator	谷口英樹横浜市立大学, 大学院・医学研究科, 教授
Project Period (FY)	2002 – 2003Completed
Keywords	ES細胞 / 細胞分化 / 再生医療
Research Abstract	胚性幹(ES)細胞の分化制御による再生医療に関する基礎的研究

Research Products of this project	Publications (9 results)

17. 造血幹細胞をニッシェに導くホーミングレセプターのクローニング

Research Project

Research Category	Grant-in-Aid for Exploratory Research
Research Field	Hematology
Research Institution	The University of Tokyo
Principal Investigator	中内啓光東京大学, 医科学研究所, 教授
Project Period (FY)	2002 – 2003Completed
Keywords	造血幹細胞 / ニッシェ / ホーミング
Research Abstract	昨年度は骨髄移植後の造血幹細胞の骨髄への到達状況について表面マーカーを利用して定量的に解析し、移植後6時間から12時間後に骨髄中に再現性を持ってGFP陽性c-Kit+Sca-1+Lin-(KSL)細胞の出現ピークが観察されることを明らかにした。また、脾臓には移植直後からGFP陽性KSL細胞の集積が認 ...

Research Products of this project	Publications (8 results)

18. Stem cell regulation for the differenntiiation of insulin-produucing cells

Research Project

Research Category	Grant-in-Aid for Scientific Research (A)
Research Field	General surgery
Research Institution	Yokohama City University
Principal Investigator	TANIGUCHI Hideki Yokohama City University Graduate School of Medicine, Department of Regenerative Medicine, Prof.
Project Period (FY)	2002 – 2004Completed
Keywords	Pancreas / Regeneration / Stem Cell / Diabetes
Research Abstract	By combining flow cytometry and clonal analysis, we show that a candidate pancreatic stem cell and progenitor population that reside in the developing ...

Research Products of this project	Journal Article (16 results) Book (13 results) Publications (12 results)

19. 骨髄幹細胞の可塑性とそのメカニズム

Research Project

Research Category	Grant-in-Aid for Scientific Research on Priority Areas
Review Section	Biological Sciences
Research Institution	The University of Tokyo
Principal Investigator	中内啓光東京大学, 医科学研究所, 教授
Project Period (FY)	2002 – 2006Completed
Keywords	造血幹細胞 / 自己複製 / bcrp-1 / Lnk / サイトカイン / Endomucin / STAT5 / TMTSP / myeloproliferative disorder / ポリコーム群タンパク / Bmi-1 / 精子幹細胞 / 自己複製能 / Repopulation Unit / Competitive Repopulation Unit / ポリコーム遺伝子群 / 非対称性分裂 / 対娘細胞解析 / blastcyst / 可塑性 / SP細胞 / 活性型STAT
Research Abstract	1)造血幹細胞の多様性と階層性の解明

Research Products of this project	Journal Article (33 results) Book (2 results) Publications (14 results)

20. 幹細胞の可塑性と未分化性維持機構

Research Project

Research Category	Grant-in-Aid for Scientific Research on Priority Areas
Review Section	Biological Sciences
Research Institution	The University of Tokyo
Principal Investigator	中内啓光 The University of Tokyo
Project Period (FY)	2002 – 2007Completed
Keywords	幹細胞 / 未分化性維持機構 / 再生医療 / テクノロジー / 未分化性 / 可塑性 / 自己複製 / 多分化能 / 造血幹細胞 / ニッシェ / ホーミング / ES細胞 / ATM / ポリコーム遺伝子産物 / 造的幹細胞 / 末分化性維持機構
Research Abstract	本特定領域研究では1)細胞の初期化の機構の解明、2)幹細胞の未分化性維持機構、3)幹細胞の多様性と可塑性の三つの柱を中心に5年間にわたり研究を進めた。ES細胞、組織幹細胞のそれぞれにおいて研究が大きく進展したが、最近2年間に本特定領域研究の分担研究者である山中伸弥教授らによって遺伝子導入によって体細 ...

Research Products of this project	Journal Article (117 results, of which Peer Reviewed: 21 results) Book (1 results) Publications (11 results)

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