KAKEN — Research Projects | Researcher Number: 80089870

Search Results: 9 results / Researcher Number: 80089870

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1. Elucidation of the structural and mechanistic basis of pollutants-and signal-transmitter-degrading dioxygenases and their directed evolution

Research Project

Research Category	Grant-in-Aid for Scientific Research on Priority Areas
Review Section	Science and Engineering
Research Institution	SHIGA UNIVERSITY OF MEDICAL SCIENCE
Principal Investigator	HORIIKE Kihachiro Shiga Univ. of Med. Sci., Department of Biochemistry, Professor
Project Period (FY)	2001 – 2003Completed
Keywords	oxygenase / reaction mechanism / non-heme iron / catechol 2,3-dioxygenase / catechol / oxygen activation / substituent effect / homoprotocatechuic acid
Research Abstract	1. Crystal structure of homoprotocatechuate 2,3-dioxygenase from Thermus thermophilus HB8 (HPCD) has been determined. Functional characterization of H ...

Research Products of this project	Publications (14 results)

2. Molecular Design and Evolution Engineering for Compositc Biochatalysts

Research Project

Research Category	Grant-in-Aid for Scientific Research on Priority Areas
Review Section	Science and Engineering
Research Institution	KYOTO UNIVERSITY
Principal Investigator	TORAYA Tetsuo Okayama Univ., Faculty of Engineering, Professor
Project Period (FY)	2001 – 2003Completed
Keywords	composite biocatalyst / coenzyme / structure activity relationship / pyridoxal phosphate / cobalamin / flavin / heme / built-in cofactor
Research Abstract	Structures and functions of composite biocatalysts were studied, and novel composite biocatalysts were constructed. Toraya et al. established a refine ...

Research Products of this project	Publications (225 results)

3. Elucidation of a novel primitive pathway of aneorobic porphyrin biosynthesis

Research Project

Research Category	Grant-in-Aid for Scientific Research (B)
Research Field	Functional biochemistry
Research Institution	Shiga University of Medical Science
Principal Investigator	ISHIDA Tetsuo Shiga University of Medical Science, Department of Biochemistry, Associate Professor
Project Period (FY)	1998 – 1999Completed
Keywords	sulfate-reducing bacteria / porphyrin / precorrin-2 / biosynthesis / oxygenase / S-adenosyl-L-methionine
Research Abstract	Sulfate-reducing bacteria are broadly found in soil of ricefield, marine sediments, wastewater biofilms, animal guts, and the bacteria play important ...

Research Products of this project	Publications (9 results)

4. An archaic pathway of porphyrin biosynthesis in prokaryotes

Research Project

Research Category	Grant-in-Aid for Scientific Research (B)
Research Field	General medical chemistry
Research Institution	Shiga University of Medical Science
Principal Investigator	SETO Akira Shiga University of Medical Science, Department of Microbiology, Professor
Project Period (FY)	1996Completed
Keywords	Porphyrin / Cytochrome c3 / Sulfate-reducing bacteria / Precorrin 2 / Uroporphyrinogen III / Coproporphyrinogen III
Research Abstract	Sulfate-reducing bacteria belonging to the genus Desulfovibrio, are included among the domain eubacteria and considered to be closely related phylogen ...

Research Products of this project	Publications (11 results)

5. カテコール体ジオキシゲナーゼの補因子にはなぜ2価鉄を3価鉄の2種があるのか

Research Project

Research Category	Grant-in-Aid for Scientific Research on Priority Areas
Research Institution	Shiga University of Medical Science
Principal Investigator	堀池喜八郎滋賀医科大学, 医学部, 教授
Project Period (FY)	1996Completed
Keywords	カテコール / オキシゲナーゼ / 酸素添加酵素 / ジオキシゲナーゼ / 二原子酸素添加酵素 / 金属酵素 / 非ヘム鉄 / 酸素の活性化
Research Abstract	カテコール環を開裂する二原子酸素添加酵素は,intradiol型とextradiol型とに分類される.前者は補因子として非ヘムFe^<3+>を含み,各種分光法の適用が容易であるが,extradiol型酵素は補因子が非ヘムFe^<2+>であり,可視部に吸収がなく,ESRも示さず,分子論的研究が遅れてい ...

Research Products of this project	Publications (3 results)

6. Does the distribution of free D-serine, which is a potent activator of the NMDA receptor complex, coincide with the localization of D-amino acid oxidase in brains?

Research Project

Research Category	Grant-in-Aid for General Scientific Research (C)
Research Field	Pathological medical chemistry
Research Institution	Shiga University of Medical Science
Principal Investigator	HORIIKE Kihachiro Shiga University of Medical Science, Department of Biochemistry I,Professor
Project Period (FY)	1994 – 1995Completed
Keywords	D-Amino Acid / D-Serine / D-Amino Acid Oxidase / Central Nervous System / Glia / Astrocyte / NMDA Receptor
Research Abstract	D-Serine is a potent activator of the NMDA receptor complex. In various brain regions of the lower vertebrate species, D-serine levels were low. On th ...

Research Products of this project	Publications (16 results)

7. Comparative biochemical studies on structure, function and physiology of catechol dioxygenases

Research Project

Research Category	Grant-in-Aid for General Scientific Research (B)
Research Field	General medical chemistry
Research Institution	Shiga University of Medical Science
Principal Investigator	NOZAKI Mitsuhiro Shiga University of Medical Science, Professor
Project Period (FY)	1990 – 1991Completed
Keywords	Oxygenase / Catechol 1, 2-dioxygenase / Catechol 2, 3-dioxygenase / 3, 5-Dichlorocatechol dioxygenase / Nonheme iron containing enzyme / omicron-Nitrophenol / Gene cloning / Charge transfer complex
Research Abstract	Catechol dioxygenases are classified into two groups : intradiol and extradiol. We have previously determined the DNA sequence of an extradiol enzyme, ...

Research Products of this project	Publications (26 results)

8. The Physiological Role of Flavin-dependent Oxidases in Mammalian Peroxisome

Research Project

Research Category	Grant-in-Aid for General Scientific Research (C)
Research Field	General medical chemistry
Research Institution	Shiga University of Medical Science
Principal Investigator	HORIIKE Kihachiro Shiga University of Medical Science, Department of Biochemistry, Associate Professor
Project Period (FY)	1988 – 1989Completed
Keywords	Peroxisome / Flavoenzyme / D-Amino acid oxidase / D-Aspartate oxidase / L-alpha-Hydroxy acid oxidase / Sulfhydryl compounds
Research Abstract	Adducts of sulfhydryl compounds and glyoxylate are substrates for the flavooxidases in peroxisome. The products can be hydrolyzed to oxalyl thiol este ...

Research Products of this project	Publications (27 results)

9. The Physiological Role of D-Amino Acid Oxidase in the Mammalian Central Nervous System

Research Project

Research Category	Grant-in-Aid for General Scientific Research (C)
Research Field	代謝生物化学
Research Institution	Shiga University of Medical Science
Principal Investigator	HORIIKE Kihachiro Shiga University of Medical Science, Associate Professor
Project Period (FY)	1985 – 1987Completed
Keywords	D-Amino acid oxidase / Flavoenzyme / Central nervous system / Thiazolidine-2-carboxylate / Coupled peroxidation method / Neuroglia / Astrocyte
Research Abstract	To elucidate the physiological role of D-amino acid oxidase (DAO), a flavin-containing peroxisomal enzyme, in the mammalian central nervous system, it ...

Research Products of this project	Publications (18 results)