| Project/Area Number |
14571142
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
SUGITANI Atushi Kyushu Univ., Kidney Care Unit, Assistant Professor, 大学病院, 講師 (00294934)
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| Co-Investigator(Kenkyū-buntansha) |
KATANO Mitsuo Kyushu Univ., Graduate school of Medical Science, Professor, 大学院・医学研究院, 教授 (10145203)
YAMAMOTO Hirofumi Kyushu Univ., Surgery and Oncology, Assistant Professor, 大学病院, 助手
井上 重隆 九州大学, 医学部附属病院, 医員
北田 秀久 九州大学, 医学部附属病院, 医員
森崎 隆 九州大学, 医学部医学研究院, 助手 (90291517)
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| Project Period (FY) |
2002 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2002: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | pancreas transplantation / kidney transplantation / warm ischemia / ischemia-reperfusion / FR167653 / cytokine / 虚血再潅流障害 / 温疎血 / 蛋白分解酵素阻害剤 / アポトーシス |
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| Research Abstract |
Background. Inflammatory cytokines are known to contribute to ischemia-reperfusion (LIR) injury. We investigated the effect of FR167653 (FR), a suppressor of interleukin (IL)-1β and tumor necrosis factor (TNF)-α, on I/R injury of the kidney in dogs.
Methods. The left kidney was subjected to ischemia for 60 minutes followed by removal of the right kidney. A control group (n=10) and an FR group (n=8) were evaluated for tissue blood flow ; resistive_index, pulsatilitv_index arterial oxygen pressure (PaO_2), serum creatinine, blood urea nitrogen (BUN), aspartate transaminase (AST), and alanine transaminase (ALT) levels ; IL-1β mRNA expression in the peripheral blood ; apoptotic index ; and histopathology.
Results. The FR group showed lower creatinine, BUN, AST, and ALT levels (p<0.038 each) : and lower IL-1β mRNA expression and apoptotic index (p<0.041 each) than did the control group. PaO_2 during 120 minutes after reperfusion in the FR group dropped but recovered quickly (p=0.024). Renal tissue damage in the FR group was less than that in the control group (p=0.036).
Conclusions. FR ameliorates I/R injury of the kidney potentially via reduced production of inflammatory cytokines that may contribute to damage to the ischeniic kidney and the distant organs.
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