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Fabrication of a microfluidic device for circulating tumor cell sensing

Research Project

Project/Area Number 15K16320
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionTokyo Medical and Dental University

Principal Investigator

TABATA Miyuki  東京医科歯科大学, 生体材料工学研究所, テニュアトラック助教 (00636839)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsバイオセンサ / 血中循環腫瘍細胞 / 乳がん
Outline of Final Research Achievements

In this research, we performed to fabricate a compact and portable device that detect circulating tumor cells (CTC) and analyze nucleic acids using ultra-sensitive biosensor based-on the principle of field effect transistors (FETs). Model breast cancer epithelial cells were immobilized on the sensor which the probe molecules were immobilized, and then electrical measurement was performed. Because cancer cells have a negative charge derived from sialic acids, we succeeded in electrically discriminating cancer cells and epithelial cells according to the difference in the amount of sialic acid expression on cell membrane. Furthermore, we tried to develop an early diagnosis device for cancer by analyzing the nucleic acid.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (44 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (30 results) (of which Int'l Joint Research: 15 results,  Invited: 2 results) Book (5 results) Remarks (1 results)

  • [Int'l Joint Research] EPFL(スイス)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Real-time Monitoring and Detection of Primer Generation-Rolling Circle Amplification of DNA Using an Ethidium Ion-selective Electrode2016

    • Author(s)
      Ayaka Seichi, Nanami Kozuka, Yuko Kashima, Miyuki Tabata, Tatsuro Goda, Akira Matsumoto, Naoko Iwasawa, Daniel Citterio, Yuji Miyahara, Koji Suzuki
    • Journal Title

      Analytical Sciences

      Volume: 32 Issue: 5 Pages: 505-510

    • DOI

      10.2116/analsci.32.505

    • NAID

      130005150940

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Measurement of Rapid Amiloride-Dependent pH Changes at the Cell Surface Using a Proton-Sensitive Field-Effect Transistor2016

    • Author(s)
      Daniel Schaffhauser, Michael Fine, Miyuki Tabata, Tatsuro Goda, Yuji Miyahara
    • Journal Title

      Biosensors

      Volume: 6 (2) Issue: 2 Pages: 11-11

    • DOI

      10.3390/bios6020011

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Label-free and Electrochemial Detection of Nucleic Acids Based on Isothermal Amplification in Combination with Solid-state pH Sensor2016

    • Author(s)
      Miyuki Tabata, Yurika Katayama, Fahmida Mannan, Ayaka Seichi, Koji Suzuki, Tatsuro Goda, Akira Matsumoto, Yuji Miyahara
    • Journal Title

      Procedia Eng.

      Volume: 168 Pages: 419-422

    • DOI

      10.1016/j.proeng.2016.11.534

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Miniaturized Ir/IrOx pH Sensor for Quantitative Diagnosis of Dental Caries2016

    • Author(s)
      Miyuki Tabata, Chindanai Ratanaporncharoen, Aoi Asano, Yuichi Kitasako, Masaomi Ikeda, Tatsuro Goda, Akira Matsumoto, Junji Tagami, Yuji Miyahara
    • Journal Title

      Procedia Eng.

      Volume: 168 Pages: 598-601

    • DOI

      10.1016/j.proeng.2016.11.223

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Real-time Monitoring and Detection of Primer Generation-rolling Circle Amplification of DNA using an Ethidium Ion Selective Electrode2016

    • Author(s)
      Ayaka Seichi, Nanami Kozuka, Yuko Kashima, Miyuki Tabata, Tatsuro Goda, Akira Matsumoto, Naoko Iwasawa, Daniel Citterio, Yuji Miyahara, Koji Suzuki
    • Journal Title

      Anal. Sci

      Volume: -

    • NAID

      130005150940

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Potentiometric Responses of Ion-Selective Microelectrode with Bovine Serum Albumin Adsorption2016

    • Author(s)
      Tatsuro Goda, Eriko Yamada, Yurika Katayama, Miyuki Tabata, Akira Matsumoto, Yuji Miyahara
    • Journal Title

      Biosens. Bioelectron.

      Volume: 77 Pages: 208-214

    • DOI

      10.1016/j.actbio.2016.02.008

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Boronate-functionalized Polymer Gel-based Insulin Delivery System with Improved Stability in Performance: A Comparative Structure–Function Study2016

    • Author(s)
      Akira Matsumoto, Mai Yuasa, Mai Sanjo, Miyuki Tabata, Tatsuro Goda, Toru Hoshi, Takao Aoyagi, Yuji Miyahara
    • Journal Title

      Chemistry Letters

      Volume: 45 Issue: 4 Pages: 460-462

    • DOI

      10.1246/cl.151177

    • NAID

      130005143983

    • ISSN
      0366-7022, 1348-0715
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Electrical detection of nucleic acid amplification for point-of-care testing2018

    • Author(s)
      Miyuki Tabata
    • Organizer
      4th Joint Symposium between IBB/TMDU and Chulalongkorn University on Biomedical Materials and Engineering
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] パターン化Ir/IrOxセンサの作製とリキッドバイオプシーへの応用2018

    • Author(s)
      田畑美幸,合田達郎,松元亮,宮原裕二,吉岡祐亮,落谷孝広
    • Organizer
      平成29年度生体医歯工学共同研究拠点成果報告会
    • Related Report
      2017 Annual Research Report
  • [Presentation] リキッドバイオプシーを指向したmicroRNA定量検出デバイス2018

    • Author(s)
      田畑美幸,吉岡祐亮,合田達郎,松元亮,落谷孝広,宮原裕二
    • Organizer
      第2回 Liquid Biopsy研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] pH検出に基づく小型核酸定量デバイスの創製2017

    • Author(s)
      田畑美幸,Enrico Tenaglia,合田達郎,松元亮,Carlotta Guiducci,宮原 裕二
    • Organizer
      生体医歯工学共同研究拠点成果報告会
    • Place of Presentation
      東京医科歯科大学(東京都文京区)
    • Year and Date
      2017-03-24
    • Related Report
      2016 Research-status Report
  • [Presentation] Miniaturized wireless pH sensor for on-site monitoring2017

    • Author(s)
      Chindanai Ratanaporncharoen,石原昇,田畑美幸,合田達郎,松元亮,益 一哉,Mana Sriyudthsak,宮原 裕二
    • Organizer
      生体医歯工学共同研究拠点成果報告会
    • Place of Presentation
      東京医科歯科大学(東京都文京区)
    • Year and Date
      2017-03-24
    • Related Report
      2016 Research-status Report
  • [Presentation] Label-free electrochemical monitoring of isothermal nucleic acid amplification using micro pH sensors2017

    • Author(s)
      Miyuki Tabata, Tatsuro Goda, Akira Matsumoto, Yuji Miyahara
    • Organizer
      4th COINS symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] abel-free microRNA detecting sensors for liquid biopsy2017

    • Author(s)
      Miyuki Tabata, Yusuke Yoshioka, Tatsuro Goda, Akira Matsumoto, Takahiro Ochiya, Yuji Miyahara
    • Organizer
      The 2nd International Symposium on Biomedical Engineering
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 第34回「センサ・マイクロマシンと応用システム」シンポジウム・参加報告(バイオ)2017

    • Author(s)
      田畑美幸
    • Organizer
      有機機能材料のリソグラフィー加工コンソーシアム第26回定例会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] pH検出に基づく核酸の増幅と検出を行う小型デバイスの創製2017

    • Author(s)
      田畑美幸,合田達郎,松元亮,宮原裕二
    • Organizer
      第34回センサ・マイクロマシンと応用システムシンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 電気化学検出による小型・可搬型核酸定量デバイスの創製2017

    • Author(s)
      田畑美幸
    • Organizer
      平成29年度E部門総合研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Label-free nucleic acid amplification detection using micro pH devices2017

    • Author(s)
      Miyuki Tabata, Enrico Tenaglia, Tatsuro Goda, Akira Matsumoto, Carlotta Guiducci, Yuji Miyahara
    • Organizer
      European Advanced Materials Congress 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] pH-based nucleic acid amplification detection on a silicon nanowire chip2016

    • Author(s)
      Tabata M, Tenaglia E, Goda T, Matsumoto A, Guiducci C, Miyahara Y
    • Organizer
      International Symposium on Biomedical Engineering
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2016-11-10
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Label-free and electrochemical detection of nucleic acids based on isothermal amplification in combination with solid-state pH sensor2016

    • Author(s)
      Tabata M, Katayama Y, Mannan F, Seichi A, Suzuki K, Goda T, Matsumoto A, Miyahara Y
    • Organizer
      Eurosensors2016
    • Place of Presentation
      Budapest, Hungary
    • Year and Date
      2016-09-04
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Miniaturized Ir/IrOx pH sensor for quantitative diagnosis of dental caries2016

    • Author(s)
      Tabata M, Ratanaporncharoen C, Asano A, Kitasako Y, Ikeda M, Goda T, Matsumoto A, Tagami J, Miyahara Y
    • Organizer
      Eurosensors2016
    • Place of Presentation
      Budapest, Hungary
    • Year and Date
      2016-09-04
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Fabrication of Micro Ir/IrOx pH Sensor for Dental Applications,2016

    • Author(s)
      Chindanai Ratanaporncharoen, Miyuki Tabata, Yuichi Kitasako, Masaomi Ikeda, Junji Tagami, Tatsuro Goda, Akira Matsumoto, Yuji Miyahara
    • Organizer
      PITTCON 2016
    • Place of Presentation
      Atlanta, USA
    • Year and Date
      2016-03-06
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 小型・簡易microRNA解析デバイス2016

    • Author(s)
      松元亮,田畑美幸,合田達郎,宮原裕二
    • Organizer
      COINS 第5回全体会議
    • Place of Presentation
      ナノ医療イノベーションセンター: iCONM(神奈川県川崎市)
    • Year and Date
      2016-01-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Development of mixed nano-structure composed of self-assembled monolayer and ultra-thin silver chloride for electrochemical biosensing2015

    • Author(s)
      Miyuki Tabata, Tatsuro Goda, Akira Matsumoto, Yuji Miyahara
    • Organizer
      The International Chemical Congress of Pacific Basin Societies 2015
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 細胞機能の非破壊定量評価と医療への応用2015

    • Author(s)
      田畑美幸,合田達郎,松元 亮,宮原裕二
    • Organizer
      ソニーサポートファンドオープンハウス
    • Place of Presentation
      東京医科歯科大学M&Dタワー26階 ファカルティラウンジ(東京都文京区)
    • Year and Date
      2015-11-24
    • Related Report
      2015 Research-status Report
  • [Presentation] Electrochemical quantitative monitoring of isothermal nucleic acid amplification2015

    • Author(s)
      Akira Matsumoto, Miyuki Tabata, Huiyong Yang, Fahmida Mannan, Yurika Katayama, Tatsuro Goda T, Ayaka Seichi, Koji Suzuki, Yuji Miyahara
    • Organizer
      2nd COINS International Symposium
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2015-11-24
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 微細加工技術による機能性電極材料の開発2015

    • Author(s)
      田畑美幸,由比藤勇,加藤邦男,関口哲史,合田達郎,松元 亮,宮原裕二
    • Organizer
      第6回6大学6研究所連研プロジェクト公開討論会
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2015-11-20
    • Related Report
      2015 Research-status Report
  • [Presentation] マイクロpHセンサを用いた等温核酸増幅の電気化学的リアルタイムモニタリング2015

    • Author(s)
      片山由梨佳,田畑美幸,合田達郎,松元 亮,星地彩花,鈴木孝治,宮原裕二
    • Organizer
      第37回日本バイオマテリアル学会大会
    • Place of Presentation
      京都テルサ(京都府京都市)
    • Year and Date
      2015-11-09
    • Related Report
      2015 Research-status Report
  • [Presentation] 自己組織化膜/塩化銀ナノ混合表面の構築と核酸検出バイオセンサへの応用2015

    • Author(s)
      田畑美幸,合田達郎,松元 亮,宮原裕二
    • Organizer
      第37回日本バイオマテリアル学会大会
    • Place of Presentation
      京都テルサ(京都府京都市)
    • Year and Date
      2015-11-09
    • Related Report
      2015 Research-status Report
  • [Presentation] 自己組織化有機単分子膜/AgClナノ混合表面の構築と核酸検出バイオセンサへの応用2015

    • Author(s)
      田畑美幸,合田達郎,松元 亮,宮原裕二
    • Organizer
      第32回「センサ・マイクロマシンと応用システム」シンポジウム
    • Place of Presentation
      朱鷺メッセ(新潟県新潟市)
    • Year and Date
      2015-10-28
    • Related Report
      2015 Research-status Report
  • [Presentation] Real-time monitoring and detection of primer generation-rolling circle amplification of DNA using an ethidium ion selective electrode2015

    • Author(s)
      Ayaka Seichi, Nanami Kozuka, Yuko Kashima, Miyuki Tabata, Tatsuro Goda, Akira Matsumoto, Naoko Iwasawa, Daniel Citterio, Yuji Miyahara, Koji Suzuki
    • Organizer
      The 16th Beijing Conference and Exhibision on Instrumental Analysis (BCEIA2015)
    • Place of Presentation
      Beijing, China
    • Year and Date
      2015-10-27
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 気化学的核酸定量デバイスの開発2015

    • Author(s)
      田畑美幸,片山由梨佳,マンナン・ファミーダ,星地彩花,鈴木孝治,合田達郎,松元亮,宮原裕二
    • Organizer
      戦略的創造研究推進事業(JST CREST・さきがけ)戦略目標「プロセスインテグレーションによる次世代ナノシステムの創製」3研究領域第3回合同公開シンポジウム
    • Place of Presentation
      品川コクヨホール(東京都港区)
    • Year and Date
      2015-09-29
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Real-time monitoring and detection of primer generation-rolling circle amplification of DNA using an ethidium ion selective electrode2015

    • Author(s)
      Ayaka Seichi, Nanami Kozuka, Yuko Kashima, Miyuki Tabata, Tatsuro Goda, Akira Matsumoto, Naoko Iwasawa, Daniel Citterio, Yuji Miyahara
    • Organizer
      RSC Tokyo International Conference 2015
    • Place of Presentation
      Chiba, Japan
    • Year and Date
      2015-09-03
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Electrochemical label-free degranulation monitoring for in-situ evaluation of cellular function2015

    • Author(s)
      Miyuki Tabata , Tatsuro Goda, Akira Matsumoto, Yuji Miyahara
    • Organizer
      the 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
    • Place of Presentation
      Milan, Italy
    • Year and Date
      2015-08-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis2015

    • Author(s)
      Miyuki Tabata, Huiyong Yang, Fahmida Mannan, Yurika Katayama, Tatsuro Goda, Akira Matsumoto Ayaka Seichi, Koji Suzuki, Yuji Miyahara
    • Organizer
      The 18th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS 2015)
    • Place of Presentation
      Anchorage, Alaska
    • Year and Date
      2015-06-22
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 小型・簡易microRNA解析デバイス2015

    • Author(s)
      松元亮,田畑美幸,合田達郎,宮原裕二
    • Organizer
      COINS 第4回全体会議
    • Place of Presentation
      ナノ医療イノベーションセンター: iCONM(神奈川県川崎市)
    • Year and Date
      2015-06-19
    • Related Report
      2015 Research-status Report
  • [Presentation] Design and fabrication of functional solid-state electrodes for liquid biopsy2015

    • Author(s)
      Miyuki Tabata, Isamu Yuitoo, Kunio Katou, Tetsushi Sekiguchi, Tatsuro Goda, Akira Matsumoto, Yuji Miyahara
    • Organizer
      The 6th International Symposium on Advanced Materials Development and Integration of Novel Structured Metallic and Inorganic Materials (AMDI-6)
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2015-06-09
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Book] がんの簡易検査を目指した体液中循環核酸の電気化学的解析技術2017

    • Author(s)
      Miyuki Tabata, Yuji Miyahara
    • Total Pages
      12
    • Publisher
      CMC publishing Co.
    • Related Report
      2017 Annual Research Report
  • [Book] Electrochemical Biosensors Combined with Isothermal Amplification for Quantitative Detection of Nucleic Acids2017

    • Author(s)
      Miyuki Tabata, Bo Yao, Ayaka Seichi, Koji Suzuki, Yuji Miyahara
    • Total Pages
      17
    • Publisher
      Springer
    • Related Report
      2017 Annual Research Report 2016 Research-status Report
  • [Book] バイオトランジスタによる生体分子計測2016

    • Author(s)
      Tatsuro Goda, Miyuki Tabata, Yuji Miyahara
    • Total Pages
      8
    • Publisher
      CMC publishing Co.
    • Related Report
      2016 Research-status Report
  • [Book] Field-Effect Transistors for Detection of Biomolecular Recognition2016

    • Author(s)
      Miyuki Tabata, Tatsuro Goda, Akira Matsumoto, Yuji Miyahara
    • Total Pages
      13
    • Publisher
      Springer
    • Related Report
      2016 Research-status Report
  • [Book] Electrical and Electrochemical Monitoring of Nucleic Acid Amplification2016

    • Author(s)
      Tatsuro Goda, Miyuki Tabata, Yuji Miyahara
    • Total Pages
      7
    • Publisher
      Frontier
    • Related Report
      2015 Research-status Report
  • [Remarks] 宮原裕二研究室 東京医科歯科大学

    • URL

      http://www.tmd.ac.jp/bsr/index.html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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