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Development of Integrated Transistor Based device for Monitoring of Enzymatic Activities as Next-generation Platform for Apoptosis Pathways Analysis

Research Project

Project/Area Number 23680051
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionTokyo Medical and Dental University

Principal Investigator

MATSUMOTO Akira  東京医科歯科大学, 生体材料工学研究所, 准教授 (70436541)

Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥28,210,000 (Direct Cost: ¥21,700,000、Indirect Cost: ¥6,510,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Keywords電界効果トランジスタ / アポトーシス / カスパーゼ / 酵素活性 / パスウェイ解析
Research Abstract

We envisioned the development of an integrated transistor based device that is able to quantify enzymatic activities in cells. Caspase-3 and -8 were studied as targets relevant to apoptosis pathway analysis. A series of peptides containing each Caspase-specific amino acid sequence along with spacer and cysteine moiety were prepared so that they will have good enzyme-accssibility and affinity to gold for the immobilization. Mass-spectroscopic analysis uncovered that these designed reactions take place in quantitative and each substrate-specific fashion. This reaction could also be visualized on the basis of the coloration of gold nano-particle. Furthermore, it was compatible with potentiometric detection format, indicating the validity of our approach.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (48 results)

All 2014 2013 2012 2011 Other

All Journal Article (15 results) (of which Peer Reviewed: 6 results,  Open Access: 2 results) Presentation (16 results) (of which Invited: 4 results) Book (12 results) Remarks (1 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 4 results)

  • [Journal Article] Fabrication of Self-assembled Monolayer/AgCl Mixed Surface and It's Electrochemical Properties2014

    • Author(s)
      Miyuki Tabata, Kozue Nogami, Tatsuro Goda, Akira Matsumoto and Yuji Miyahara
    • Journal Title

      IEEJ Transactions on Sensors and Micromachines

      Volume: 134(10)(in press)

    • NAID

      130004693998

    • Related Report
      2013 Final Research Report
  • [Journal Article] Chemo-electrical Signal Transduction by using Stimuli-responsive Polymer Gate-modified Field Effect Transistor2014

    • Author(s)
      Akira Matsumoto; Yusuke Tsurui; Hiroko Matsumoto; Yasuhiro Maeda; Toru Hoshi; Takashi Sawaguchi; Yuji Miyahara
    • Journal Title

      Chemosensors

      Volume: 2(2) Pages: 97-107

    • Related Report
      2013 Final Research Report
  • [Journal Article] Phenylboronic acids-based Diagnostic and Therapeutic Applications2014

    • Author(s)
      Mai Sanjo, Kazunori Kataoka, Yuji Miyahara, Akira Matsumoto
    • Journal Title

      Analytical Science

      Volume: 30(1) Pages: 111-117

    • NAID

      130004442231

    • Related Report
      2013 Final Research Report
  • [Journal Article] Chemo-electrical Signal Transduction by using Stimuli-responsive Polymer Gate-Modified Field Effect Transistor2014

    • Author(s)
      Akira Matsumoto, Yusuke Tsurui, Hiroko Matsumoto, Yasuhiro Maeda, Toru Hoshi, Takashi Sawaguchi, and Yuji Miyahara
    • Journal Title

      Chemosensors

      Volume: 2(2) Issue: 2 Pages: 97-107

    • DOI

      10.3390/chemosensors2020097

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phenylboronic Acids-based Diagnostic and Therapeutic Applications2014

    • Author(s)
      Mai Sanjoh, Yuji Miyahara, Kazunori Kataoka, Akira Matsumoto
    • Journal Title

      Analytical Sciences

      Volume: 30 Issue: 1 Pages: 111-117

    • DOI

      10.2116/analsci.30.111

    • NAID

      130004442231

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Simple and Robust Strategy for Potentiometric Detection of Glucose Using Fluorinated Phenylboronic Acid Self-assembled Monolayer2013

    • Author(s)
      Akira Matsumoto; Hiroko Matsumoto; Yuji Miyahara
    • Journal Title

      BBA-Gen. Subjects

      Volume: 1830(9) Pages: 4359-4364

    • Related Report
      2013 Final Research Report
  • [Journal Article] Current and Emerging Challenges of Field Effect Transistor Based Bio-sensing2013

    • Author(s)
      Akira Matsumoto, Yuji Miyahara
    • Journal Title

      Nonoscale

      Volume: 5(22) Pages: 10702-10718

    • Related Report
      2013 Final Research Report
  • [Journal Article] Simple and Robust Strategy for Potentiometric Detection of Glucose Using Fluorinated Phenylboronic Acid Self-assembled Monolayer2013

    • Author(s)
      Akira Matsumoto, Hiroko Matsumoto, Yasuhiro Maeda and Yuji Miyahara
    • Journal Title

      BBA - Gen. Subjects

      Volume: 1830(9) Issue: 9 Pages: 4359-4364

    • DOI

      10.1016/j.bbagen.2013.03.004

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Current and emerging challenges in field effect transistor based bio-sensing2013

    • Author(s)
      Akira Matsumoto, Yuji Miyahara
    • Journal Title

      Nanoscale

      Volume: 5(22) Issue: 22 Pages: 10702-10718

    • DOI

      10.1039/c3nr02703a

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosensing technology2013

    • Author(s)
      Akira Matsumoto, Yuji Miyahara
    • Journal Title

      Jinkouzouki

      Volume: 42(3) Pages: 213-216

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Biotransistor with Functional Nanointerface Containing Biomolecules2013

    • Author(s)
      Miyuki Tabata, Tatsuro Goda, Akira Matsumoto, Yuji Miyahara
    • Journal Title

      IEEJ Transactions on Sensors and Micromachines

      Volume: 133 Issue: 11 Pages: B314-B319

    • DOI

      10.1541/ieejsmas.133.B314

    • NAID

      130003382691

    • ISSN
      1341-8939, 1347-5525
    • Related Report
      2013 Annual Research Report
  • [Journal Article] Glucose-Responsive Gel for Self-Regulated Insulin Delivery System2013

    • Author(s)
      Akira Matsumoto, Takehiko Ishii, Kazunori Kataoka, Yuji Miyahara
    • Journal Title

      Drug Delivery System

      Volume: 28(2) Pages: 119-126

    • NAID

      130003368339

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Preparation of a-Mannoside Hydrogel and Electrical Detection of Saccharide-Protein Interactions Using the Smart Gel-modified gate Field Effect Transistor2012

    • Author(s)
      Yasuhiro Maeda, Akira Matsumoto, Yoshiko Miura, Yuji Miyahara
    • Journal Title

      Nanoscale Res. Lett.

      Volume: 7 Pages: 108-108

    • Related Report
      2013 Final Research Report
  • [Journal Article] Preparation of alpha-Mannoside Hydrogel and Electrical Detection of Saccharide-protein Interactions using the Smart Gel-modified Gate Field Effect Transistor2012

    • Author(s)
      Yasuhiro Maeda
    • Journal Title

      Nanoscale Res. Lett.

      Volume: 7 Issue: 1 Pages: 108-108

    • DOI

      10.1186/1556-276x-7-108

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Current Development Status and Perspective of Self-Regulated Insulin Delivery Systems: A Review2012

    • Author(s)
      Akira Matsumoto, Yuji Miyahara
    • Journal Title

      IEEJ Transactions on Sensors and Micromachines

      Volume: 132 Issue: 12 Pages: 455-458

    • DOI

      10.1541/ieejsmas.132.455

    • NAID

      130002510568

    • ISSN
      1341-8939, 1347-5525
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] penylboronate based strategies for drug delivery and biosensing, nanotechnology platform2013

    • Author(s)
      Akira Matsumoto
    • Organizer
      Japan-USA young scientist exchange program
    • Place of Presentation
      Tsukuba
    • Year and Date
      2013-12-09
    • Related Report
      2013 Final Research Report
  • [Presentation] ボロン酸を利用した生体計測とDDS2013

    • Author(s)
      松元亮
    • Organizer
      「ナノメディシン分子科学」第三回若手研究交流会
    • Place of Presentation
      東京
    • Year and Date
      2013-10-10
    • Related Report
      2013 Final Research Report
  • [Presentation] 半導体/生体分子ナノ界面の構築とバイオトランジスタへの応用2013

    • Author(s)
      荒井貴裕、鶴井祐輔、前田康弘、合田達郎、松元亮、宮原裕二
    • Organizer
      ソフトインターフェースの分子科学第10回公開シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] フェニルボロン酸含有高分子層を検出界面とするシアル酸認識バイオトランジスタへの創製2013

    • Author(s)
      荒井貴裕、前島雪絵、合田達郎、松元亮、大塚英典、宮原裕二
    • Organizer
      電気学会バイオ・マイクロシステム研究会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] Phenylboronate based strategies for drug delivery and biosensing2013

    • Author(s)
      Matsumoto A.
    • Organizer
      Nanotechnology platform: Japan-USA young scientist exchange program
    • Place of Presentation
      Tsukuba
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ボロン酸を利用した生体計測とDDS2013

    • Author(s)
      松元亮
    • Organizer
      ナノメディシン分子科学」第3回若手研究交流会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 半導体/生体分子ナノ界面の構築とバイオトランジスタへの応用2013

    • Author(s)
      荒井貴裕,鶴井祐輔,前田康弘,合田達郎,松元亮,宮原裕二
    • Organizer
      ソフトインターフェースの分子科学 第10回公開シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] フェニルボロン酸含有高分子層を検出界面とするシアル酸認識バイオトランジスタの創製2013

    • Author(s)
      荒井貴裕,前島雪絵,合田達郎,松元亮,大塚英典,宮原裕二
    • Organizer
      電気学会 バイオ・マイクロシステム研究会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] Sialic acid sensitive field effect transistor as a means of facile and quantitative cytology2011

    • Author(s)
      Akira Matsumoto, Kazunori Kataoka, Yuji Miyahara
    • Organizer
      2011 MRS Fall Meeting & Exhibit
    • Place of Presentation
      Boston
    • Year and Date
      2011-11-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Gel-based Field Effect Transistor for Lectin-detection2011

    • Author(s)
      Yasuhiro Maeda, Akira Matsumoto, Keiko Miura, Yuji Miyahara
    • Organizer
      The International Conference on Thin Films (ICTF15)
    • Place of Presentation
      Kyoto
    • Year and Date
      2011-11-09
    • Related Report
      2013 Final Research Report
  • [Presentation] ボロン酸を利用した生体計測とDDS2011

    • Author(s)
      松元亮
    • Organizer
      「スマートバイオプロジェクト」セミナー
    • Place of Presentation
      大阪
    • Year and Date
      2011-10-20
    • Related Report
      2013 Final Research Report
  • [Presentation] 半導体とバイオの融合—バイオトランジスタの開発—2011

    • Author(s)
      松元亮、宮原裕二
    • Organizer
      半導体・集積回路技術シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2011-07-08
    • Related Report
      2013 Final Research Report
  • [Presentation] 半導体とバイオの融合 ―バイオトランジスタの開発―

    • Author(s)
      松元亮、宮原裕二
    • Organizer
      半導体・集積回路技術シンポジウム
    • Place of Presentation
      早稲田大学(東京都)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Sialic acid sensitive field effect transistor as a means of facile and quantitative cytology

    • Author(s)
      松元亮、片岡一則、宮原裕二
    • Organizer
      2011 MRS Fall Meeting & Exhibit
    • Place of Presentation
      Boston, USA
    • Related Report
      2011 Annual Research Report
  • [Presentation] ボロン酸を利用した生体計測とDDS

    • Author(s)
      松元亮
    • Organizer
      「スマートバイオプロジェクト」セミナー
    • Place of Presentation
      関西大学(大阪府)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Gel-based Field Effect Transistor for Lectin-detection

    • Author(s)
      前田康弘、松元亮、三浦佳子、宮原裕二
    • Organizer
      The International Conference on Thin Films (ICTF15)
    • Place of Presentation
      京都テルサ(京都府)
    • Related Report
      2011 Annual Research Report
  • [Book] "Development of a Totally Synthetic"Artificial Pancreas"by Using Smart Gels", in Human Sensing for Smart Life Care–Sensor, Information & Energy Technologies on Health Conscious Society with Big Data- (ed.: K. Mitsubayashi)2014

    • Author(s)
      Akira Matsumoto, Yuji Miyahara
    • Publisher
      CMC Publishing Co., Ltd., Japan
    • Related Report
      2013 Final Research Report
  • [Book] Human Sensing for Smart Life Care -Sensor, Information & Energy Technologies on Health Conscious Society with Big Data-2014

    • Author(s)
      Akira Matsumoto, Yuji Miyahara
    • Total Pages
      8
    • Publisher
      CMC publishing Co., Japan
    • Related Report
      2013 Annual Research Report
  • [Book] Biosensing2013

    • Author(s)
      Akira Matsumoto, Yuji Miyahara
    • Publisher
      Artificial Organ
    • Related Report
      2013 Final Research Report
  • [Book] Glucose-Responsive Gel for Self-Regulated Insulin Delivery System, 28(2)2013

    • Author(s)
      Akira Matsumoto, Takehiko Ishii, Kazunori Kataoka, Yuji Miyahara
    • Publisher
      Drug Delivery Systems
    • Related Report
      2013 Final Research Report
  • [Book] Current Development Status and Perspective of Self-Regulated Insulin Delivery Systems : A Review, 132(12)2012

    • Author(s)
      Akira Matsumoto, Yuji Miyahara
    • Publisher
      IEEJ Transactions on Sensors and Micromachines
    • Related Report
      2013 Final Research Report
  • [Book] "intelligent surfaces for solid-state nano-biosensing devices", in intelligent Surfaces in Biotechnology : Scientific and Engineering Concepts, Enabling Technologies, and Translation to Bio-Oriented Applications (ed.: H. Michelle Grandin, Marcus Textor)2012

    • Author(s)
      Akira Matsumoto, Yuji Miyahara, Kazunori Kataoka
    • Publisher
      John Wiley & Sons, Inc., USA
    • Related Report
      2013 Final Research Report
  • [Book] Intelligent Surfaces in Biotechnology: Scientific and Engineering Concepts, Enabling Technologies, and Translation to Bio-Oriented Applications2012

    • Author(s)
      Akira Matsumoto, Yuji Miyahara, kazunori Kataoka
    • Total Pages
      8
    • Publisher
      John Wiley & Sons, Inc., USA
    • Related Report
      2012 Annual Research Report
  • [Book] "Trends and Perspective of Biosensors", 80(3)(in Oyo Butsuri)2011

    • Author(s)
      Akira Matsumoto, Yuji Miyahara
    • Related Report
      2013 Final Research Report
  • [Book] "New Trends in NanoBio-Sensing" , 26(1)2011

    • Author(s)
      Akira Matsumoto, Yuji Miyahara
    • Publisher
      Drug Delivery Systems
    • Related Report
      2013 Final Research Report
  • [Book] 応用物理:「バイオセンサの現状と今後の展望」2011

    • Author(s)
      松元亮、宮原裕二
    • Total Pages
      6
    • Publisher
      応用物理学会
    • Related Report
      2011 Annual Research Report
  • [Book] DDS:ナノバイオセンシングの新展開2011

    • Author(s)
      松元亮、宮原裕二
    • Total Pages
      5
    • Publisher
      日本DDS学会
    • Related Report
      2011 Annual Research Report
  • [Book] Intelligent surfaces: polymeric coatings for applications in bio-related and life sciences2011

    • Author(s)
      Akira Matsumoto, Yuji Miyahara, Kazunori Kataoka
    • Total Pages
      6
    • Publisher
      Wiley & Sons Inc.
    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.tmd.ac.jp/bsr/index.html

    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 検出デバイス及びバイオセンサ2014

    • Inventor(s)
      松元亮、宮原裕二
    • Industrial Property Rights Holder
      権利者:独立行政法人物質・材料研究機構
    • Industrial Property Rights Type
      特許
    • Acquisition Date
      2014-03-11
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 検出デバイス及びバイオセンサ2013

    • Inventor(s)
      松元亮、宮原裕二
    • Industrial Property Rights Holder
      国立大学法人東京大学、独立行政法 人物質・材料研究機構
    • Industrial Property Rights Type
      特許
    • Acquisition Date
      2013-11-01
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 生体分子検出用電極チップ、及び、生体分子検出方法2012

    • Inventor(s)
      前田康弘、合田達郎、宮原裕二、松 元亮、片岡知歩
    • Industrial Property Rights Holder
      国立大学法人東京医科歯科大学、独立行政法人物質・材料研究機構
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-06-11
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 生体分子検出用電極チップ、及び、生体分子検出方法2011

    • Inventor(s)
      前田康弘、合田達郎、宮原裕二、松 元亮、片岡知歩
    • Industrial Property Rights Holder
      国立大学法人東京医科歯科大学、独立行政法人物質・材料研究機構
    • Industrial Property Rights Type
      特許
    • Filing Date
      2011-04-20
    • Related Report
      2013 Final Research Report
    • Overseas

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Published: 2011-04-06   Modified: 2019-07-29  

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