Cell Chemical Biology
Volume 23, Issue 11, 17 November 2016, Pages 1341-1350
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Article
Allosteric Inhibition of a Semaphorin 4D Receptor Plexin B1 by a High-Affinity Macrocyclic Peptide

https://doi.org/10.1016/j.chembiol.2016.09.015Get rights and content
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Highlights

  • A plexin B1-binding cyclic peptide was isolated from a highly diverse library

  • The peptide inhibited function of plexin B1 in cellular assay

  • Crystal structure of peptide-plexin complex was determined

  • The structure revealed non-competitive nature of the inhibition

Summary

Semaphorin axonal guidance factors are multifunctional proteins that play important roles in immune response, cancer cell proliferation, and organogenesis, making semaphorins and their signaling receptor plexins important drug targets for various diseases. However, the large and flat binding surface of the semaphorin-plexin interaction interface is difficult to target by traditional small-molecule drugs. Here, we report the discovery of a high-affinity plexin B1 (PlxnB1)-binding macrocyclic peptide, PB1m6 (KD = 3.5 nM). PB1m6 specifically inhibited the binding of physiological ligand semaphorin 4D (Sema4D) in vitro and completely suppressed Sema4D-induced cell collapse. Structural studies revealed that PB1m6 binds at a groove between the fifth and sixth blades of the sema domain in PlxnB1 distant from the Sema4D-binding site, indicating the non-competitive and allosteric nature of the inhibitory activity. The discovery of this novel allosteric site can potentially be used to target plexin family proteins for the development of drugs that modulate semaphorin and plexin signaling.

Keywords

semaphorin
plexin
macrocyclic peptide
allosteric inhibitor

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