Dermal Vγ4(+) γδ T cells possess a migratory potency to the draining lymph nodes and modulate CD8(+) T-cell activity through TNF-α production.
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Abstract
A large number of gamma delta T cells (γδ T cells) are located within epithelial tissues including the skin. In mice, epidermal and dermal γδ T cells consist of distinct subsets and have specific roles in cutaneous immune responses. A recent study demonstrated that γδ T cells and cutaneous dendritic cells migrate from the skin to the draining lymph nodes (LNs). However, it remains unclear whether they regulate the antigen-specific immune response within the LNs. Herein, we investigated their properties and role in the LNs using the Mycobacterium bovis bacille Calmette-Guérin (BCG) infection model. In vivo cell labeling analysis revealed that most of the migratory subset comprised dermal Vγ4(+) cells. This population transmigrated from the skin to the LNs in a Gi-coupled chemokine receptor-independent manner. By depleting Vγ4(+) cells, the intranodal expansion of CD8(+) T cell against BCG was significantly attenuated. In addition, in vitro analysis revealed that Vγ4(+) cells produced TNF-α and enhanced IL-12 production by dendritic cells. Taken together, these findings suggest that dermal Vγ4(+) cells are a unique subset that possesses a migratory potency to the skin-draining LNs and enhances the dendritic cell function therein.
Journal
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- The Journal of investigative dermatology
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The Journal of investigative dermatology 135 (4), 1007-1015, 2015-01-08
Nature Publishing Group
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Keywords
- Animals
- CD8-Positive T-Lymphocytes/cytology
- Cell Movement
- Cell Proliferation
- Gene Expression Regulation
- Interferon-gamma/metabolism
- Interleukin-12/metabolism
- Langerhans Cells/cytology
- Langerhans Cells/metabolism
- Light
- Lymph Nodes/metabolism
- Lymph Nodes/pathology
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Mycobacterium bovis/metabolism
- Receptors, Antigen, T-Cell, gamma-delta/metabolism
- Skin/immunology
- T-Lymphocyte Subsets/immunology
- Tumor Necrosis Factor-alpha/metabolism
Details
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- CRID
- 1050564285766911360
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- NII Article ID
- 120005621945
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- NII Book ID
- AA00700733
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- ISSN
- 0022202X
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- HANDLE
- 2433/198677
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- IRDB
- CiNii Articles