Differentiation ability of multipotent hematopoietic stem/progenitor cells detected by a porcine specific anti-CD117 monoclonal antibody

  • Ohshima Shino
    Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine
  • Mori Syuuya
    Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine
  • Shigenari Atsuko
    Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine
  • Miyamoto Asuka
    Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine
  • Takasu Masaki
    Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
  • Imaeda Noriaki
    Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
  • Nunomura Satoshi
    Division of Molecular Cell Immunology, Nihon University Graduate School of Medical Science
  • Okayama Yoshimichi
    Division of Molecular Cell Immunology, Nihon University Graduate School of Medical Science
  • Tanaka Masafumi
    Division of Molecular Cell Immunology, Nihon University Graduate School of Medical Science
  • Kitagawa Hitoshi
    Division of Molecular Cell Immunology, Nihon University Graduate School of Medical Science
  • Kulski Jerzy K.
    Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine Centre for Forensic Science, The University of Western Australia
  • Inoko Hidetoshi
    Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine
  • Ando Asako
    Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine
  • Kametani Yoshie
    Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine

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Abstract

CD117 is a cytokine receptor expressed on the surface of hematopoietic stem cells with a likely role in cell survival, proliferation and differentiation. In order to study the differentiation activity of porcine CD117 hematopoietic cells in vitro and in vivo we prepared an anti-swine CD117 Mab (2A1) with high specificity for flow-cytometrical analysis. The 2A1 Mab did not recognize mouse or human mast cells suggesting that 2A1 is species-specific. Swine bone marrow (BM) CD117+ cells differentiated in vitro mainly into erythroid and monocyte lineages in the methylcellulose-based colony assay. When the swine BM CD117+ cells were transplanted in vivo into immunodeficient NOG (NOD/SCID/IL-2gc-null) mice, a significant amount of swine CD45+ leukocytes, including CD3 positive T cells, were developed in the mice. These results revealed that the swine BM CD117+ cells possess hematopoietic stem/progenitor activity and when monitored in immunodeficient mice or in vitro they can develop into lymphoid, erythroid, and myeloid cells efficiently with the new monoclonal antibody.

Journal

  • BioScience Trends

    BioScience Trends 8 (6), 308-315, 2014

    International Research and Cooperation Association for Bio & Socio-Sciences Advancement

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