Gene and protein analysis of brain derived neurotrophic factor expression in relation to neurological recovery induced by an enriched environment in a rat stroke model
Highlights
► Postischemic enriched environment improved motor function and increased synapse. ► RT-PCR, Western blotting, and immunostaining measured mature BDNF and proBDNF. ► No significant difference was detected in BDNF mRNA or BDNF proteins. ► ProBDNF, a pro-apoptotic factor, was first measured in association with enrichment. ► Improved function cannot be ascribed to decrease in proBDNF.
Section snippets
Acknowledgements
This study was partially supported by Special Coordination Funds for Promoting Science and Technology of the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government. This research was also partially supported by a Grant-in-Aid for General Scientific Research from the Japan Society for the Promotion of Science.
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