Support for international research in "epigenome dynamics and regulation in germ cells"

• Project Area: Analyses and regulation of germline epigenome
• Project/Area Number: 15K21737
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Multi-year Fund
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: Takashi Shinohara 京都大学, 医学研究科, 教授 (30322770)
• Co-Investigator(Kenkyū-buntansha): 伊川 正人 大阪大学, 微生物病研究所, 教授 (20304066) ; 小倉 淳郎 国立研究開発法人理化学研究所, バイオリソースセンター, 室長 (20194524)
• Project Period (FY): 2015-11-06 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥44,460,000 (Direct Cost: ¥34,200,000、Indirect Cost: ¥10,260,000); Fiscal Year 2017: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000); Fiscal Year 2016: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000); Fiscal Year 2015: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
• Keywords: エピジェネティクス / 精子形成 / 卵子形成 / 核移植 / 顕微受精 / 生殖細胞

Outline of Final Research Achievements

For international collaboration, researchers from six countries have visited Japan. Eight of 10 researchers in this grant group member wrote international collaboration papers. We also supported an international collaboration for the analysis of human embryonic germ cells by providing travel expense to the proposed researcher. For supporting young researchers, we provided international travel funds to X young researchers who wanted to go to international meetings. However, these researchers were selected and awarded "best presentater" during summer retreat. All members who participated in the retreat voted to select best researchers. In addition, we also selected a total of X best papers from all the papers published in each year. The first authors of these papers were also supported for their travel expense to join international conferences.

Research Products

• [Int'l Joint Research] Hawaii大学/Baylor College of Medicine(米国)
• [Int'l Joint Research] Utrecht 大学(オランダ)
• [Int'l Joint Research] Teramo大学(イタリア)
• [Int'l Joint Research] Institute of Molecular Genetics(チェコスロバキア)
• [Int'l Joint Research] Max Planck Institute(ドイツ)
• [Int'l Joint Research]
• [Int'l Joint Research] University of Texas/Pittsburgh University/Baylor College of Medicine(米国)
• [Int'l Joint Research] McGill University(カナダ)
• [Int'l Joint Research] Teramo University(イタリア)
• [Int'l Joint Research] Institute of Molecular Genetics(チェコスロバキア)
• [Int'l Joint Research] National Seoul University(韓国)
• [Int'l Joint Research]
• [Int'l Joint Research] Baylor College of Medicine(米国)
• [Int'l Joint Research] Teramo University(イタリア)
• [Int'l Joint Research] Institute of Molecular Genetics(チェコスロバキア)
• [Int'l Joint Research] University of Texas at San Antonio(米国)
• [Int'l Joint Research] 国立ソウル大学(韓国)
• [Journal Article] TCTE1 is a conserved component of the dynein regulatory complex and is required for motility and metabolism in mouse spermatozoa. (2017)
  • Author(s): Castaneda JM, Hua R, Miyata H, Oji A, Guo Y, Cheng Y, Zhou T, Guo X, Cui Y, Shen B, Wang Z, Hu Z, Zhou Z, Sha J, Prunskaite-Hyyrylainen R , Yu Z, Ramirez-Solis R, Ikawa M, Matzuk MM and Liu M
  • Journal Title: Proc Natl Acad Sci U S A Volume: 114(27) Issue: 27
  • DOI: 10.1073/pnas.1621279114
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Reprogramming towards totipotency is greatly facilitated by synergistic effects of small molecules (2017)
  • Author(s): Miyamoto K, Tajima Y, Yoshida K, Oikawa M, Azuma R, Allen GE, Tsujikawa T, Tsukaguchi T, Bradshaw CR, Jullien J, Yamagata K, Matsumoto K, Anzai M, Imai H, Gurdon JB, Yamada M
  • Journal Title: Biology Open Volume: 6 Issue: 4 Pages: 415-424
  • DOI: 10.1242/bio.023473
  • NAID: 120006223660
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Gene Resistance to Transcriptional Reprogramming following Nuclear Transfer Is Directly Mediated by Multiple Chromatin-Repressive Pathways (2017)
  • Author(s): Jullien J, Vodnala M, Pasque V, Oikawa M, Miyamoto K, Allen G, David SA, Brochard V, Wang S, Bradshaw C, Koseki H, Sartorelli V, Beaujean N, Gurdon J
  • Journal Title: Molecular Cell Volume: 65 Issue: 5 Pages: 873-884
  • DOI: 10.1016/j.molcel.2017.01.030
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis (2017)
  • Author(s): Ishiguro K., Monti M., Akiyama T., Kimura H., Chikazawa-Nohtomi N., Sakota M., Sato S., Redi CA., Ko SBH., Ko MSH.
  • Journal Title: In Vitro Cell.Dev.Biol.-Anim. Volume: 53 Issue: 2 Pages: 167-178
  • DOI: 10.1007/s11626-016-0096-z
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Long non-coding RNA exchange during the oocyte-to-embryo transition in mice. (2017)
  • Author(s): Karlic R, Ganesh S, Franke V, Svobodova E, Urbanova J, Suzuki Y, Aoki F, Vlahovicek K, Svoboda P.
  • Journal Title: DNA Res Volume: 24 Pages: 129-141
  • DOI: 10.1093/dnares/dsw058
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] De novo DNA methylation drives 5hmC accumulation in mouse zygotes. (2016)
  • Author(s): Amouroux R, Nashun B, Shirane K, Nakagawa S, Hill PW, D'Souza Z, Nakayama M, Matsuda M, Turp A, Ndjetehe E, Encheva V, Kudo NR, Koseki H, Sasaki H, Hajkova P.
  • Journal Title: Nat. Cell Biol. Volume: 18 Issue: 2 Pages: 225-233
  • DOI: 10.1038/ncb3296
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Behavior of Mouse Spermatozoa in the Female Reproductive Tract from Soon after Mating to the Beginning of Fertilization. (2016)
  • Author(s): Muro Y, Hasuwa H, Isotani A, Miyata H, Yamagata K, Ikawa M, Yanagimachi R, Okabe M.
  • Journal Title: Biol Reprod. Volume: 1 Pages: 1-1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Calreticulin Regulates Neointima Formation and Collagen Deposition following Carotid Artery Ligation. (2016)
  • Author(s): Zimmerman KA, Xing D, Pallero MA, Lu A, Ikawa M, Black L, Hoyt KL, Kabarowski JH, Michalak M, *Murphy-Ullrich JE.
  • Journal Title: J Vasc Res. Volume: 25 Pages: 306-320
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Fertilization defects in sperm from Cysteine-rich secretory protein 2 (Crisp2) knockout mice: Implications for fertility disorders. (2016)
  • Author(s): Brukman NG, Miyata H, Torres P, Lombardo D, Caramelo JJ, Ikawa M, Da Ros VG, Cuasnicú PS.
  • Journal Title: Mol Hum Reprod. Volume: 1 Pages: 1-1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Max as a biological blockade that restricts meiotic process in ESCs. (2016)
  • Author(s): Suzuki A, Hirasaki M, Hishida T, Okamura D, Wu J, Ueda A, Nishimoto M, Nakachi Y, Mizuno Y, Okazaki Y, Matsui Y, *Izpisua Belmonte JC, Okuda A.
  • Journal Title: Nat Commun Volume: 7 Issue: 1 Pages: 11056-11056
  • DOI: 10.1038/ncomms11056
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Elf5-centered transcription factor hub controls trophoblast stem cell self-renewal and differentiation through stoichiometry-sensitive shifts in target gene networks. (2015)
  • Author(s): Latos PA, Sienerth AR, Murray A, Senner CE, Muto M, Ikawa M, Oxley D, Burge S, Cox BJ, Hemberger M.
  • Journal Title: Genes Dev. Volume: 29 Issue: 23 Pages: 2435-48
  • DOI: 10.1101/gad.268821.115
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CRISPR/Cas9-Mediated Rapid Generation of Multiple Mouse Lines Identified Ccdc63 as Essential for Spermiogenesis (2015)
  • Author(s): Samantha AM Young, Haruhiko Miyata, Yuhkoh Satouh, Hirotaka Kato, Kaori Nozawa, Ayako Isotani, R John Aitken, Mark A Baker, Masahito Ikawa
  • Journal Title: Int. J. Mol. Sci Volume: 16 Issue: 10 Pages: 24732-24750
  • DOI: 10.3390/ijms161024732
  • NAID: 120006957408
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Calreticulin is required for development of the cumulus oocyte complex and female fertility. (2015)
  • Author(s): Tokuhiro K, Satouh Y, Nozawa K, Isotani A, Fujihara Y, Hirashima Y, Matsumura H, Takumi K, Miyano T, Okabe M, Benham AM, Ikawa M.
  • Journal Title: Sci Rep. Volume: 5 Issue: 1 Pages: 14254-14254
  • DOI: 10.1038/srep14254
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] APJ Regulates Parallel Alignment of Arteries and Veins in the Skin. (2015)
  • Author(s): Kidoya H, Naito H, Muramatsu F, Yamakawa D, Jia W, Ikawa M, Sonobe T, Tsuchimochi H, Shirai M, Adams RH, Fukamizu A, Takakura N.
  • Journal Title: Dev Cell. Volume: 33 Issue: 3 Pages: 247-259
  • DOI: 10.1016/j.devcel.2015.02.024
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Quantitative assessment of telomerase components in cancer cell lines. (2015)
  • Author(s): Akıncılar SC, Low KC, Liu CY, Yan TD, Oji A, Ikawa M, Li S, Tergaonkar V.
  • Journal Title: FEBS Lett. Volume: 589 Issue: 9 Pages: 1-1
  • DOI: 10.1016/j.febslet.2015.02.035
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT) (2015)
  • Author(s): Ohtsuka M., Miura H., Mochida K., Hirose M., Hasegawa A., Ogura A., Mizutani R., Kimura M., Isotani A., Ikawa M., Sato M. and Gurumurthy C. B.
  • Journal Title: BMC Genomics Volume: 16 Issue: 1 Pages: 274-274
  • DOI: 10.1186/s12864-015-1432-5
  • NAID: 120006957412
  • Peer Reviewed / Open Access
• [Journal Article] A Simple and Robust Method for Establishing Homogeneous Mouse Epiblast Stem Cell Lines by Wnt Inhibition (2015)
  • Author(s): Sugimoto M, Kondo M, Koga Y, Shiura H, Ikeda R, Hirose M, Ogura A, Murakami A, Yoshiki A, Chuva de Sousa Lopes SM, Abe K
  • Journal Title: Stem Cell Reports Volume: 4 Issue: 4 Pages: 744-757
  • DOI: 10.1016/j.stemcr.2015.02.014
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Selective Targeting of the KRAS Codon 12 Mutation Sequence by Pyrrole–Imidazole Polyamide seco-CBI Conjugates. (2015)
  • Author(s): Taylor RD, Chandran A, Kashiwazaki G, Hashiya K, Bando T, Nagase H and Sugiyama H.
  • Journal Title: Chemistry. Volume: 21 Issue: 42 Pages: 14996-5003
  • DOI: 10.1002/chem.201501870
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The first murine zygotic transcription is promiscuous and uncoupled from splicing and 3' processing. (2015)
  • Author(s): Abe, K., Yamamoto, R., Franke, V., Cao, M., Suzuki, Y., Suzuki, M.G., Vlahovicek, K., Svoboda, P., Schultz, R.M. and Aoki, F.
  • Journal Title: EMBO J. Volume: 34 Issue: 11 Pages: 1523-1537
  • DOI: 10.15252/embj.201490648
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ren Y, Suzuki H, Jagarlamudi K, Golnoski K, McGuire M, Lopes R, Pachnis V, Rajkovic A.* (2015)
  • Author(s): Ren Y, Suzuki H, Jagarlamudi K, Golnoski K, McGuire M, Lopes R, Pachnis V, Rajkovic A.
  • Journal Title: BMC Biol. Volume: 13 Issue: 1 Pages: 39-39
  • DOI: 10.1186/s12915-015-0151-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Functional crosstalk between Myc and PI3K signaling for supporting unlimited self-renewal of embryonic stem cells (2015)
  • Author(s): Hishida T, Nakachi Y, Mizuno Y, Katano M, Okazaki Y, Ema M, Takahashi S, Hirasaki M, Suzuki A, Ueda A, Nishimoto M, Hishida-Nozaki Y, Vazquez-Ferrer E, Sancho-Martinez I, Izpisua Belmonte JC, Okuda A.
  • Journal Title: STEM CELLS Volume: 33 Issue: 3 Pages: 713-725
  • DOI: 10.1002/stem.1893
  • Peer Reviewed / Open Access
• [Presentation] Sperm Calcineurin is Necessary for Midpiece Flexibility and Male Fertility (2016)
  • Author(s): 宮田治彦
  • Organizer: Biophysical Society 60th Annual Meeting
  • Place of Presentation: Los Angeles
  • Year and Date: 2016-02-27
  • Int'l Joint Research