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Ensuring integrity of gametogenesis

Administrative Group

Project AreaEnsuring integrity in gametogenesis
Project/Area Number 18H05544
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionKyushu University

Principal Investigator

HAYASHI Katsuhiko  九州大学, 医学研究院, 教授 (20287486)

Co-Investigator(Kenkyū-buntansha) 北島 智也  国立研究開発法人理化学研究所, 生命機能科学研究センター, チームリーダー (00376641)
栗本 一基  奈良県立医科大学, 医学部, 教授 (20415152)
中村 隼明  広島大学, 統合生命科学研究科(生), 准教授 (30613723)
木村 啓志  東海大学, マイクロ・ナノ研究開発センター, 教授 (40533625)
吉田 松生  基礎生物学研究所, 生殖細胞研究部門, 教授 (60294138)
吉崎 悟朗  東京海洋大学, 学術研究院, 教授 (70281003)
尾畑 やよい  東京農業大学, 生命科学部, 教授 (70312907)
小林 悟  筑波大学, 生存ダイナミクス研究センター, 教授 (90225508)
小林 俊寛  東京大学, 医科学研究所, 特任准教授 (20587414)
Project Period (FY) 2018-06-29 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥91,780,000 (Direct Cost: ¥70,600,000、Indirect Cost: ¥21,180,000)
Fiscal Year 2022: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2021: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2020: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2019: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2018: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Keywords配偶子 / 生殖細胞 / 配偶子形成 / 体外培養 / 幹細胞 / 生殖細胞系列
Outline of Final Research Achievements

The following achievements were mainly made in the activities of this research group. (1) Two research meetings were held annually to promote the exchange of the latest information among all research groups. One of these meetings was held as a meeting for young researchers, which was organized by young researchers. (2) Continuous supports were made for conducting omics analyses, sharing data servers with all research groups, and supplying microdevices for culture systems developed by research groups. (3) Continuous supports were made for dispatching young researchers to overseas research meetings. Although the pandemic situation of the new coronavirus interfered with this activity, we continued to support online participation in overseas research meetings. (4) International symposiums were held and 187 people (from 6 countries) participated in person, and 239 people (from 22 countries) participated online.

Academic Significance and Societal Importance of the Research Achievements

総括班の活動により、領域研究の成果を最大限に引き出すことができた。領域研究の研究成果は国内外に発信されて、日本の生殖細胞研究のプレゼンスの向上に貢献した。これと同時に若手研究者が育成され、次世代の当該学術領域の継続的な発展が見込まれた。また領域の研究成果をメディアや市民講座などを通して、わかりやすく国民に説明することにより、当該研究分野が広く認知されている。特に不妊治療等に関する社会的な注目度は高く、本研究領域が発信した研究成果は、不妊治療に関する技術的または倫理的課題に問題を提起する機会が多くあった。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (59 results)

All 2023 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (17 results) Journal Article (19 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 19 results,  Open Access: 16 results) Presentation (19 results) (of which Int'l Joint Research: 10 results,  Invited: 14 results) Remarks (2 results) Funded Workshop (2 results)

  • [Int'l Joint Research] Max Delbrick Center (MDC)/The Leibniz Institute (IZW)(ドイツ)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Suranaree University of Technology(タイ)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] German Cancer Research Center(ドイツ)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] The Gurdon Institute(英国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Max Delbrick Center (MDC)/The Leibniz Institute (IZW)(ドイツ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Suranaree University of Technology(タイ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] German Cancer Research Center(ドイツ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] The Gurdon Institute(英国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Max Delbrick Center (MDC)/The Leibniz Institute (IZW)(ドイツ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Suranaree University of Technology(タイ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] German Cancer Research Center(ドイツ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Max Delbrick Center (MDC)/The Leibniz Institute (IZW)(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Suranaree University of Technology(タイ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] German Cancer Research Center(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Max Delbrick Center (MDC)/The Leibniz Institute (IZW)(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Suranaree University of Technology(タイ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] German Cancer Research Center(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Induction of primordial germ cell-like cells from common marmoset embryonic stem cells by inhibition of WNT and retinoic acid signaling2023

    • Author(s)
      Shono Mayumi、Kishimoto Keiko、Hikabe Orie、Hayashi Masafumi、Semi Katsunori、Takashima Yasuhiro、Sasaki Erika、Kato Kiyoko、Hayashi Katsuhiko
    • Journal Title

      Scientific Reports

      Volume: 13 Issue: 1 Pages: 3186-3186

    • DOI

      10.1038/s41598-023-29850-z

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Generation of functional oocytes from male mice in vitro2023

    • Author(s)
      Murakami Kenta、Hamazaki Nobuhiko、Hamada Norio、Nagamatsu Go、Okamoto Ikuhiro、Ohta Hiroshi、Nosaka Yoshiaki、Ishikura Yukiko、Kitajima Tomoya S.、Semba Yuichiro、Kunisaki Yuya、Arai Fumio、Akashi Koichi、Saitou Mitinori、Kato Kiyoko、Hayashi Katsuhiko
    • Journal Title

      Nature

      Volume: 615 Issue: 7954 Pages: 900-906

    • DOI

      10.1038/s41586-023-05834-x

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Controlled X‐chromosome dynamics defines meiotic potential of female mouse <i>in vitro</i> germ cells2022

    • Author(s)
      Severino Jacqueline、Bauer Moritz、Mattimoe Tom、Arecco Niccolo、Cozzuto Luca、Lorden Patricia、Hamada Norio、Nosaka Yoshiaki、Nagaoka So I、Audergon Pauline、Tarruell Antonio、Heyn Holger、Hayashi Katsuhiko、Saitou Mitinori、Payer Bernhard
    • Journal Title

      The EMBO Journal

      Volume: 41 Issue: 12 Pages: 1-23

    • DOI

      10.15252/embj.2021109457

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Reconstitution of reproductive organ system that produces functional oocytes2022

    • Author(s)
      Hayashi Katsuhiko、Yoshino Takashi
    • Journal Title

      Current Opinion in Genetics &amp; Development

      Volume: 77 Pages: 101982-101982

    • DOI

      10.1016/j.gde.2022.101982

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Robust induction of primordial germ cells of white rhinoceros on the brink of extinction2022

    • Author(s)
      Hayashi Masafumi、Zywitza Vera、Naitou Yuki、Hamazaki Nobuhiko、Goeritz Frank、Hermes Robert、Holtze Susanne、Lazzari Giovanna、Galli Cesare、Stejskal Jan、Diecke Sebastian、Hildebrandt Thomas B.、Hayashi Katsuhiko
    • Journal Title

      Science Advances

      Volume: 8 Issue: 49

    • DOI

      10.1126/sciadv.abp9683

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dual role of <i>Ovol2</i> on the germ cell lineage segregation during gastrulation in mouse embryogenesis2022

    • Author(s)
      Naitou Yuki、Nagamatsu Go、Hamazaki Nobuhiko、Shirane Kenjiro、Hayashi Masafumi、Hayashi Makoto、Kobayashi Satoru、Hayashi Katsuhiko
    • Journal Title

      Development

      Volume: 149 Issue: 4 Pages: 1-13

    • DOI

      10.1242/dev.200319

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Na?ve-like pluripotency to pave the way for saving the northern white rhinoceros from extinction2022

    • Author(s)
      Zywitza V、Rusha E、Shaposhnikov D、Ruiz-Orera J、Telugu N、Rishko V、Hayashi M、Michel G、Wittler、Stejskal J、Holtze S、Goritz F、Hermes R、Wang J、Izsvak Z、Colleoni S、Lazzari G、Galli C、Hildebrandt TB.、Hayashi K、Diecke S、Drukker M
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 3100-3100

    • DOI

      10.1038/s41598-022-07059-w

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Generation of ovarian follicles from mouse pluripotent stem cells2021

    • Author(s)
      Yoshino Takashi、Suzuki Takahiro、Nagamatsu Go、Yabukami Haruka、Ikegaya Mika、Kishima Mami、Kita Haruka、Imamura Takuya、Nakashima Kinichi、Nishinakamura Ryuichi、Tachibana Makoto、Inoue Miki、Shima Yuichi、Morohashi Ken-ichirou、Hayashi Katsuhiko
    • Journal Title

      Science

      Volume: 373 Issue: 6552 Pages: 282-289

    • DOI

      10.1126/science.abe0237

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mammalian in vitro gametogenesis2021

    • Author(s)
      Saitou Mitinori、Hayashi Katsuhiko
    • Journal Title

      Science

      Volume: 374 Issue: 6563

    • DOI

      10.1126/science.aaz6830

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reconstitution of the oocyte transcriptional network with transcription factors2021

    • Author(s)
      Hamazaki N, Kyogoku H, Araki H, Miura F, Horikawa C, Hamada N, Shimamoto S, Hikabe O, Nakashima K, Kitajima TS, Ito T, Leitch HG, Katsuhiko Hayashi.
    • Journal Title

      Nature

      Volume: 589 Issue: 7841 Pages: 883-897

    • DOI

      10.1038/s41586-020-3027-9

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control2021

    • Author(s)
      Matsumura Takafumi、Sato Takuya、Abe Takeru、Sanjo Hiroyuki、Katagiri Kumiko、Kimura Hiroshi、Fujii Teruo、Tanaka Hiromitsu、Hirabayashi Masumi、Ogawa Takehiko
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 3458-3458

    • DOI

      10.1038/s41598-021-82792-2

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Blocking estrogen-induced AMH expression is crucial for normal follicle formation2021

    • Author(s)
      Tanimoto Ren、Sekii Kiyono、Morohaku Kanako、Li Jianzhen、Pepin David、Obata Yayoi
    • Journal Title

      Development

      Volume: 148 Issue: 6

    • DOI

      10.1242/dev.197459

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats2021

    • Author(s)
      Toshihiro Kobayashi, Teppei Goto, Mami Oikawa, Makoto Sanbo, Fumika Yoshida, Reiko Terada, Naoko Niizeki, Naoyo Kajitani, Kanako Kazuki, Yasuhiro Kazuki, Shinichi Hochi, Hiromitsu Nakauchi, M. Azim Surani & Masumi Hirabayashi
    • Journal Title

      Nature Comminications

      Volume: 12 Issue: 1 Pages: 13281-10

    • DOI

      10.1038/s41467-021-21557-x

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Stable kinetochore-microtubule attachments restrict MTOC position and spindle elongation in oocytes2021

    • Author(s)
      Courtois Aurelien、Yoshida Shuhei、Takenouchi Osamu、Asai Kohei、Kitajima Tomoya S
    • Journal Title

      EMBO reports

      Volume: 22 Issue: 4

    • DOI

      10.15252/embr.202051400

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Absence of X-chromosome dosage compensation in the primordial germ cells of Drosophila embryos2021

    • Author(s)
      Ota Ryoma、Hayashi Makoto、Morita Shumpei、Miura Hiroki、Kobayashi Satoru
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 4890-4890

    • DOI

      10.1038/s41598-021-84402-7

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Environmental factors for establishment of the dormant state in oocytes2020

    • Author(s)
      Hayashi Katsuhiko、Shimamoto So、Nagamatsu Go
    • Journal Title

      Development, Growth & Differentiation

      Volume: 62 Issue: 3 Pages: 150-157

    • DOI

      10.1111/dgd.12653

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Germline development in rat revealed by visualization and deletion of Prdm142020

    • Author(s)
      Kobayashi Toshihiro、Kobayashi Hisato、Goto Teppei、Takashima Tomoya、Oikawa Mami、Ikeda Hiroki、Terada Reiko、Yoshida Fumika、Sanbo Makoto、Nakauchi Hiromitsu、Kurimoto Kazuki、Hirabayashi Masumi
    • Journal Title

      Development

      Volume: 147 Pages: 183798-183798

    • DOI

      10.1242/dev.183798

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro reconstitution of germ cell development†2019

    • Author(s)
      Hayashi Katsuhiko
    • Journal Title

      Biology of Reproduction

      Volume: 101 Issue: 3 Pages: 567-578

    • DOI

      10.1093/biolre/ioz111

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Embryos and embryonic stem cells from the white rhinoceros.2018

    • Author(s)
      Hildebrandt TB, Hermes R, Colleoni S, Diecke S, Holtze S, Renfree MB, Stejskal J, Hayashi K, Drukker M, Loi P, Groitz F, Lazzari G, Galli C.
    • Journal Title

      Nat Commun.

      Volume: 9 Issue: 1 Pages: 2589-2589

    • DOI

      10.1038/s41467-018-04959-2

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Reconstitution of gametogenesis using pluripotent stem cells.2022

    • Author(s)
      Katsuhiko Hayashi
    • Organizer
      The 14th Annual Conference of The Oncofertility Consortium
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Reconstitution of molecular and cellular network regulating oogenesis2022

    • Author(s)
      Katsuhiko Hayashi
    • Organizer
      55th Annual Meeting of JSDB
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Reconstitution of Gametogenesis using Pluripotent Stem Cells2022

    • Author(s)
      Katsuhiko Hayashi
    • Organizer
      GRC Mammalian Reproduction
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Reconstitution of reproductive organs including germ cells using mouse pluripotent stem cells2022

    • Author(s)
      Katsuhiko Hayashi
    • Organizer
      10th GSCN Conference
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Reconstitution of gametogenesis using pluripotent stem cells2022

    • Author(s)
      Katsuhiko Hayashi
    • Organizer
      Cold Spring Harbor Laboratory Meeting
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] In vitro gametogenesis2022

    • Author(s)
      Katsuhiko Hayashi
    • Organizer
      Fertility2022
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Frontiers of in vitro gametogenesis2021

    • Author(s)
      Katsuhiko Hayashi
    • Organizer
      ISSCR
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Reconstitution of ovarian follicles using mouse pluripotent stem cells2021

    • Author(s)
      Katsuhiko Hayashi
    • Organizer
      ISSCR
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 卵子の再生技術と今後の展開2021

    • Author(s)
      林 克彦
    • Organizer
      第61回日本臨床化学会年次学術集会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 哺乳類卵子の形成メカニズムの解明と再構築2021

    • Author(s)
      林 克彦
    • Organizer
      第44回 日本分子生物学会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 生殖細胞形成ネットワークの構築2021

    • Author(s)
      林克彦
    • Organizer
      日本エピジェネティクス研究会年会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Artificially produced gametes in mice, humans and other species2021

    • Author(s)
      HAYASHI, Katsuhiko
    • Organizer
      International Embryo Technology Society(
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Oocyte production in culture toward application to species conservation2021

    • Author(s)
      HAYASHI, Katsuhiko
    • Organizer
      International Society for Stem Cell Research(
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Conservation and diversity of germline development in mammals2020

    • Author(s)
      KOBAYASHI, Toshihiro
    • Organizer
      1st CU- KU Symposium and 4th CU-NIPS Symposium
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 卵母細胞の休止状態が保たれるメカニズム2019

    • Author(s)
      林克彦
    • Organizer
      第91回日本遺伝学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 精子幹細胞の集団動態と次世代へ伝わる変異へのインパクト2019

    • Author(s)
      吉田松生
    • Organizer
      第91回日本遺伝学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ショウジョウバエ生殖系列の品質管理機構2019

    • Author(s)
      小林覚
    • Organizer
      第91回日本遺伝学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] A mechanism underlying sustainable oocyte production in mouse germ line2019

    • Author(s)
      林克彦
    • Organizer
      第42回日本分子生物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Robust spermatogenesis is supported by random behavior and simple regulation of stem cells2019

    • Author(s)
      吉田松生
    • Organizer
      第42回日本分子生物学会
    • Related Report
      2019 Annual Research Report
  • [Remarks] 2018年度採択新学術領域研究  配偶子インテグリティの構築

    • URL

      https://www.gamete-integrity.com/

    • Related Report
      2022 Annual Research Report
  • [Remarks] 配偶子インテグリティの構築

    • URL

      https://www.gamete-integrity.com

    • Related Report
      2018 Annual Research Report
  • [Funded Workshop] The International Symposium "Totipotency and Germ Cell Development"2022

    • Related Report
      2022 Annual Research Report
  • [Funded Workshop] BDR symposium 2019 Control and Design of Biosystems2019

    • Related Report
      2018 Annual Research Report

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Published: 2018-07-20   Modified: 2024-01-30  

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