• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Replication, repair and transcription; coupling mechanisms and chromatin dynamics for genome integrity

Administrative Group

Project AreaCoupling of replication, repair and transcription, and their common mechanism of chromatin remodeling
Project/Area Number 22131001
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionGakushuin University

Principal Investigator

Hanaoka Fumio  学習院大学, 理学部, 研究員 (50012670)

Co-Investigator(Kenkyū-buntansha) IKURA Tsuyoshi  京都大学, 放射線生物研究センター, 准教授 (70335686)
Co-Investigator(Renkei-kenkyūsha) SEKI Masayuki  東北薬科大学, 薬学部, 教授 (70202140)
URA Kiyoe  千葉大学, 大学院理学研究科, 教授 (80289363)
YASUI Akira  東北大学, 加齢医学研究所, 教授 (60191110)
KOHNO Takashi  国立がん研究センター, 研究所, 分野長 (80280783)
YAMAGATA Yuriko  熊本大学, 生命科学研究部, 教授 (40183678)
TANAKA Kiyoji  大阪大学, 生命機能研究科, 特任教授 (80144450)
IDE Hiroshi  広島大学, 理学研究科, 教授 (30223126)
Project Period (FY) 2010-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥39,260,000 (Direct Cost: ¥30,200,000、Indirect Cost: ¥9,060,000)
Fiscal Year 2014: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2013: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2012: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2011: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2010: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywordsゲノム / DNA複製 / DNA修復 / 転写 / ヒストン修飾 / クロマチンリモデリング / 領域運営 / 国際発信 / 発がん / ゲノム再編 / DNA修復 / 複製
Outline of Final Research Achievements

DNA metabolisms such as replication, repair and transcription all utilize chromatin structure and are functionally coupled with each other. We aimed at promoting the activities of the group research to clarify the common mechanism of chromatin remodeling and to create a new paradigm of these molecular dynamics. We promoted: 1) efficient management of the research group, 2) international visualization of our research, 3) improvement of the research management based on external evaluation, 4) inter-disciplinary and collaborative researches between related research areas and support of meetings, and 5) support for career of young investigators.

Report

(6 results)
  • 2015 Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (50 results)

All 2016 2015 2014 2013 2012 2011 2010 Other

All Int'l Joint Research (2 results) Journal Article (22 results) (of which Peer Reviewed: 22 results,  Open Access: 10 results,  Acknowledgement Compliant: 9 results) Presentation (20 results) (of which Int'l Joint Research: 3 results,  Invited: 18 results) Book (1 results) Remarks (3 results) Funded Workshop (2 results)

  • [Int'l Joint Research] NIDDK, NIH/Stony Brook University/NIA, NIH(米国)

    • Related Report
      2014 Annual Research Report
  • [Int'l Joint Research] Erasmus University/Leiden University(オランダ)

    • Related Report
      2014 Annual Research Report
  • [Journal Article] UV-induced mutations in epidermal cells of mice defective in DNA polymerase η and/or ι2015

    • Author(s)
      Kanao R, Yokoi M, Ohkumo T, Sakurai Y, Dotsu K, Kura S, Nakatsu Y, Tsuzuki T, Masutani C, Hanaoka F
    • Journal Title

      DNA Repair (Amsterdam)

      Volume: S1568-7864(15) Pages: 00042-7

    • DOI

      10.1016/j.dnarep.2015.02.006

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] SUMOylation of xeroderma pigmentosum group C protein regulates DNA damage recognition during nucleotide excision repair2015

    • Author(s)
      Akita M
    • Journal Title

      Sci. Rep.

      Volume: 5 Issue: 1 Pages: 10984-10984

    • DOI

      10.1038/srep10984

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The protein level of Rev1, a TLS polymerase in fission yeast, is strictly regulated during the cell cycle and after DNA damage.2015

    • Author(s)
      Uchiyama M, Terunuma J, Hanaoka F
    • Journal Title

      PLoS One

      Volume: 10 Issue: 7 Pages: e0130000-e0130000

    • DOI

      10.1371/journal.pone.0130000

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Different types of interaction between PCNA and PIP boxes contribute to distinct cellular functions of Y-family DNA polymerases.2015

    • Author(s)
      Masuda Y, Kanao R, Kaji K, Ohmori H, Hanaoka F, Masutani C
    • Journal Title

      Nucleic Acids Res

      Volume: 43 Issue: 16 Pages: 7898-7910

    • DOI

      10.1093/nar/gkv712

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] USP7 is a suppressor of PCNA ubiquitination and oxidative-stress-induced mutagenesis in human cells.2015

    • Author(s)
      Kashiwaba S, Kanao R, Masuda Y, Kusumoto-Matsuo R, Hanaoka F, Masutani C
    • Journal Title

      Cell Rep

      Volume: 13 Issue: 10 Pages: 2072-2080

    • DOI

      10.1016/j.celrep.2015.11.014

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A cyclobutane thymine-N4-methylcytosine dimer is resistant to hydrolysis but strongly blocls DNA synthesis.2014

    • Author(s)
      Yamamoto J, Oyama T, Kunishi T, Masutani C, Hanaoka F, Iwai S
    • Journal Title

      Nucleic Acids Res

      Volume: 42 Issue: 3 Pages: 2075-2084

    • DOI

      10.1093/nar/gkt1039

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Histone chaperone FACT regulates homologous recombination by chromatin remodeling through interaction with RNF20.2014

    • Author(s)
      Oliveira DV, Kato A, Nakamura K, Ikura T, Okada M, Kobayashi J, Yanagihara H, Saito Y, Tauchi H, Komatsu K
    • Journal Title

      J Cell Sci

      Volume: 127 Pages: 763-772

    • DOI

      10.1242/jcs.135855

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Trapping DNA replication origins from the human genome.2013

    • Author(s)
      Eki T, Murakami Y, Hanaoka F
    • Journal Title

      Genes (Basel)

      Volume: 4 Issue: 2 Pages: 198-225

    • DOI

      10.3390/genes4020198

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Mechanism of somatic hypermutation at the WA motif by human DNA polymerase eta.2013

    • Author(s)
      Zhao Y, Gregory MT, Biertümpfel C, Hua YJ, Hanaoka F, Yang W
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 110 Issue: 20 Pages: 8146-8151

    • DOI

      10.1073/pnas.1303126110

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Structure-function analysis of the EF-hand protein centrin-2 for its intracellular localization and nucleotide excision repair.2013

    • Author(s)
      Nishi R, Sakai W, Tone D, Hanaoka F, Sugasawa K
    • Journal Title

      Nucleic Acids Res

      Volume: 41 Issue: 14 Pages: 6917-6929

    • DOI

      10.1093/nar/gkt434

    • NAID

      120005621886

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of new scavengers for hydroxyl radicals and superoxide dismutase by utilising ultraviolet A photoreaction of 8-methoxysoralen and a variety of mutants of Escherichia coli: implications on certain diseases of DNA repair deficiency.2012

    • Author(s)
      Ahmad SI
    • Journal Title

      J Photochem Photobiol B

      Volume: 116 Pages: 30-36

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis of human DNA polymerase eta-mediated chemoresistance to cisplatin.2012

    • Author(s)
      Zhao Y
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 109 Issue: 19 Pages: 7269-7274

    • DOI

      10.1073/pnas.1202681109

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A DNA oligomer containing 2,2,4-triamino-5(2H)-oxazolone is incised by human NEIL1 and NTH1.2012

    • Author(s)
      Kino K
    • Journal Title

      Mutat Res

      Volume: 734 Issue: 1-2 Pages: 73-77

    • DOI

      10.1016/j.mrfmmm.2012.03.007

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The vital role of polymerase ζ and REV1 in mutagenic, but not correct, DNA synthesis across benzo[a]pyrene-dG and recruitment of polymerase ζ by REV1 to replication-stalled site.2012

    • Author(s)
      Hashimoto K
    • Journal Title

      J Biol Chem

      Volume: 287 Issue: 12 Pages: 9613-9622

    • DOI

      10.1074/jbc.m111.331728

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stalled Polη at its cognate substrate initiates an alternative translesion synthesis pathway via interaction with REV1.2012

    • Author(s)
      Ito W
    • Journal Title

      Genes Cells

      Volume: 17 Issue: 2 Pages: 98-108

    • DOI

      10.1111/j.1365-2443.2011.01576.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of mice deficient in both REV1 catalytic activity and POLH reveals an unexpected role for POLH in the generation of C to G and G to C transversions during Ig gene hypermutation.2012

    • Author(s)
      Kano C
    • Journal Title

      Int Immunol

      Volume: 24 Issue: 3 Pages: 169-174

    • DOI

      10.1093/intimm/dxr109

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The molecular basis of CRL4DDB2/CSA ubiquitin ligase architecture, targeting, and activation2011

    • Author(s)
      Fischer, E.S., et al
    • Journal Title

      Cell

      Volume: 147 Issue: 5 Pages: 1024-1039

    • DOI

      10.1016/j.cell.2011.10.035

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] NBS1 recruits RAD18 via aRAD6-like domain and regulates Polη-dependent translesion DNA synthesis2011

    • Author(s)
      Yanagihara H, Kobayashi J, Tateishi S, Kato A, Matsuura S, Tauchi H, Yamada K, Takezawa J, Sugasawa K, Masutani C, Hanaoka F, Weemaes CM, Mori T, Zou L, Komatsu K.
    • Journal Title

      Mol Cell

      Volume: 43 Issue: 5 Pages: 788-797

    • DOI

      10.1016/j.molcel.2011.07.026

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular chaperone Hsp90 regulates REV1-mediated mutagenesis.2011

    • Author(s)
      Pozo FM
    • Journal Title

      Mol Cell Biol

      Volume: 31 Issue: 16 Pages: 3396-3409

    • DOI

      10.1128/mcb.05117-11

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic analysis using a mouse cell cycle mutant identifies magoh as a novel gene involved in Cdk regulation2011

    • Author(s)
      Inaki, M., et al
    • Journal Title

      Genes Cells

      Volume: 16 Pages: 166-178

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Polymerization by DNA polymerase eta is blocked by cis-diamminedichloroplatinum (II) 1,3-d (GpTpG) cross-link : implications for cytotoxic effects in nucleotide excision repair-negative tumor cells2010

    • Author(s)
      Chijiwa, S., et al
    • Journal Title

      Carcinogenesis

      Volume: 31 Pages: 388-393

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ATM modulates the loading of recombination proteins onto a chromosomal translocation breakpoint hotspot2010

    • Author(s)
      Sun, J., et al
    • Journal Title

      PLoS ONE

      Volume: 5

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 総括2015

    • Author(s)
      花岡文雄
    • Organizer
      BMB2015 ワークショップ「放射線生物影響の課題に挑む分子生物学研究の力」
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 私のDNA修復研究:XPCからXPVへ2015

    • Author(s)
      花岡文雄
    • Organizer
      新学術領域研究「ゲノム普遍的制御」終了シンポジウム:ゲノム安定性の機構と生命の維持 -進化、癌化、老化の理解のために
    • Place of Presentation
      京都
    • Year and Date
      2015-08-29
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Structural and biochemical analyses for accommodation and recognition of UV-damaged bases within nucleosome.2015

    • Author(s)
      Osakabe A, Tachiwana H, Kagawa W, Horikoshi N, Matsumoto S, Yamamoto J, Hanaoka F, Sugasawa K, Iwai S, Kurumizaka H
    • Organizer
      「クロマチン動構造」国際シンポジウム
    • Place of Presentation
      淡路島、兵庫
    • Year and Date
      2015-08-23
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulation of the protein level of Rev1 in fission yeast.2015

    • Author(s)
      Uchiyama M, Terunuma J, Hanaoka F
    • Organizer
      Genomic Integrity, ZING Conference 2015
    • Place of Presentation
      Cairns, Australia
    • Year and Date
      2015-08-01
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Forty years of DNA damage tolerance.2015

    • Author(s)
      Hanaoka F
    • Organizer
      15th International Congress of Radiation Research (ICRR 2015)
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2015-05-25
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 損傷トレランスと突然変異生成に働く損傷乗り越え合成ポリメラーゼ2014

    • Author(s)
      花岡文雄
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本
    • Year and Date
      2014-03-28 – 2014-03-30
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Defective translesion DNA synthesis and links to human disease.2014

    • Author(s)
      Hanaoka F
    • Organizer
      International Symposium on Xeroderma Pigmentosum and Related Diseases: Disorders of DNA Damage Response -Bench to Bedside-
    • Place of Presentation
      神戸
    • Year and Date
      2014-03-05 – 2014-03-07
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Role of DNA polymerase eta in somatic hypermutation.2013

    • Author(s)
      Hanaoka F
    • Organizer
      3rd France-Japan Cancer Meeting
    • Place of Presentation
      Toulouse, France
    • Year and Date
      2013-11-20 – 2013-11-23
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] がんとは何か?ーその発生のメカニズムを探るー2013

    • Author(s)
      花岡文雄
    • Organizer
      読売サイエンスフォーラム
    • Place of Presentation
      学習院大学
    • Year and Date
      2013-07-27
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Structure and function of mammalian DNA polymerase eta with special reference on translesion synthesis and somatic hypermutation.2013

    • Author(s)
      Hanaoka F
    • Organizer
      Seminar at Graduate School of Medicine, Kyoto University
    • Place of Presentation
      京都大学医学部
    • Year and Date
      2013-05-27
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Involvement of DNA polymerase eta-REV1 interaction in a part of error-prone translesion DNA synthesis.2012

    • Author(s)
      Hanaoka F
    • Organizer
      The 4th U.S.-Japan DNA Repair Meeting
    • Place of Presentation
      Virginia, USA
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Functional roles of mammalian DNA polymerase eta, the product of the XP-V responsible gene.2012

    • Author(s)
      Hanaoka F
    • Organizer
      The 3rd Erling Seeberg Symposium on DNA Repair
    • Place of Presentation
      Trondheim and Orland, Norway
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Alternative translesion synthesis pathway via interaction between stalled DNA polymerase eta and REV12012

    • Author(s)
      Hanaoka F
    • Organizer
      The 8th 3R Symposium together with National Institute of Genetics “2012 International Symposium”: “Molecular Mechanisms and Pathology of the 3R”
    • Place of Presentation
      淡路市、兵庫
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Functional roles of mammalian DNA polymerase eta2012

    • Author(s)
      Hanaoka F
    • Organizer
      2nd Japanese-Frence Cancer Workshop
    • Place of Presentation
      鳴門市、徳島
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] DNA修復と疾患:色素性乾皮症を中心に2011

    • Author(s)
      花岡文雄
    • Organizer
      第28回日本医学会総会
    • Place of Presentation
      東京
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Biochemical properties and structure of human DNA polymerase η.2011

    • Author(s)
      Hanaoka, F.
    • Organizer
      6th UK-Japan Cell Cycle Workshop
    • Place of Presentation
      Lake Windermere, UK
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] DNA修復研究における生化学的アプローチ2011

    • Author(s)
      花岡文雄
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会議場
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Biochemical properties and structure of human DNA polymerase &eta, xeroderma pigmentosum variant-responsible gene product2011

    • Author(s)
      Hanaoka, F.
    • Organizer
      GDRI France Japan Conference
    • Place of Presentation
      Montpellier, France
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Tumorigenesis induced by chronic treatment with UV-B in Pol eta- and X-pc-deficient mice2010

    • Author(s)
      花岡文雄
    • Organizer
      Gordon Research Conference on Mutagenesis
    • Place of Presentation
      Waterville, Maine, USA(招待講演)
    • Year and Date
      2010-08-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] DNAポリメラーゼ・イータ ―その構造と多彩な機能―

    • Author(s)
      花岡文雄
    • Organizer
      新学術領域研究「遺伝情報場」公開シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] DNA Replication, Recombination, and Repair: Molecular Mechanisms and Pathology2016

    • Author(s)
      Hanaoka F, Sugasawa K
    • Total Pages
      555
    • Publisher
      Springer, Japan
    • Related Report
      2014 Annual Research Report
  • [Remarks] 学習院大学 花岡研究室

    • URL

      http://www-cc.gakushuin.ac.jp/~20080213

    • Related Report
      2014 Annual Research Report
  • [Remarks] 学習院大学 花岡研究室

    • URL

      http://www-cc.gakushuin.ac.jp/~20080213/

    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://www.dnarepair.jp/

    • Related Report
      2010 Annual Research Report
  • [Funded Workshop] 第9回3Rシンポジウム2014

    • Place of Presentation
      御殿場市、静岡県
    • Year and Date
      2014-11-17
    • Related Report
      2014 Annual Research Report
  • [Funded Workshop] 第5回日米DNA修復会議2014

    • Place of Presentation
      鳴門市、徳島県
    • Year and Date
      2014-10-28
    • Related Report
      2014 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi