| Budget Amount *help |
¥35,880,000 (Direct Cost: ¥27,600,000、Indirect Cost: ¥8,280,000)
Fiscal Year 2019: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2017: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
We found that cytochrome P450BM3 starts to catalyze hydroxylation of nonnative substrates in the presence of inert dummy substrates (decoy molecules). Recently, we have demonstrated that various carboxylic acids modified with amino acids (N-acyl amino acids) having a completely different structure from fatty acids can serve as decoy molecules. Benzene was more efficiently hydroxylated in the presence of these decoy molecules. We also have demonstrated that the heme acquisition protein HasA secreted by Pseudomonas aeruginosa can accommodate Iron(III)-5,15-diphenylporphyrin and its derivatives including Fe-diaza-DPP without any structural perturbation. Crystal structure analysis revealed that phenyl groups at the meso-position of the porphyrins extend outside of HasA to avoid steric crowding and are exposed to the solvent. Iron(III)- and cobalt(III)-tetraphenylporphycenes, which possess bulky phenyl groups, also can be incorporated into HasA.
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