| Budget Amount *help |
¥96,070,000 (Direct Cost: ¥73,900,000、Indirect Cost: ¥22,170,000)
Fiscal Year 2019: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2018: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2015: ¥28,470,000 (Direct Cost: ¥21,900,000、Indirect Cost: ¥6,570,000)
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| Outline of Final Research Achievements |
Membrane lipid bilayer plays important functions not only as physical barrier of cells/organelles but also as signal platforms. We here investigated the role of lipid microdomains in inflammatory responses using newly established probes which specifically bind to phosphatidyl serine or sphingomyelin (SM). We found that SM was detected in the cytosolic leave of membranes of trans-golgi network, recycling endosomes and lysosomes. SM-enriched membrane domains in trans-golgi network recruited STING and TBK1, and mediated STING-dependent type I Interferon productions. STING palmitoylation was critical for its localization to SM-rich golgi membrane. We also found that SM-rich domain in lysosomes was constituted depending on MHC class I and its cis-interacting C-type lectin, and this domain played important role for macrophage phagocytosis. Our results revealed that SM-enriched membrane microdomains in organelle provide important platforms for transmitting various inflammatory signals.
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