|Budget Amount *help
¥103,480,000 (Direct Cost: ¥79,600,000、Indirect Cost: ¥23,880,000)
Fiscal Year 2019: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2018: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2017: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2016: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2015: ¥29,120,000 (Direct Cost: ¥22,400,000、Indirect Cost: ¥6,720,000)
|Outline of Final Research Achievements
The most important our research result is development of precise temperature shift assay, and finding that individual transport pathways have different sensitivities to a rise in temperature. Hikeshi mediated nuclear transport of Hsp70 occur at much lower temperature than the inhibition of Ran dependent transport, and among Ran dependent transport pathway, importin α/β dependent import ceases at a lower temperature than other Ran-dependent transport, showing these two transport pathways are affected at fever-ranged mild heat shock condition instead of severe heat shock condition. The transport activity raises or drops in narrow temperature range, suggesting physiological importance of their regulation.
Dysfunction of Hikeshi influences various biological events irrespective of stress or normal condition, due to lack of nuclear Hsp70. We expect nuclear Hsp70 is essential for nuclear proteostasis, a process that have not been carefully investigated before.