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Regulatory mechanism of intracellular temperature sensing and energy metabolism

Planned Research

Project AreaIntegrative understanding of biological phenomena with temperature as a key theme
Project/Area Number 15H05930
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionKyoto University

Principal Investigator

UMEDA Masato  京都大学, 工学研究科, 教授 (10185069)

Project Period (FY) 2015-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥103,610,000 (Direct Cost: ¥79,700,000、Indirect Cost: ¥23,910,000)
Fiscal Year 2019: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2018: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2017: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2016: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
Fiscal Year 2015: ¥29,510,000 (Direct Cost: ¥22,700,000、Indirect Cost: ¥6,810,000)
Keywords生理学 / 酵素 / 脂質 / 発現制御 / 昆虫 / 体温調節 / エネルギー代謝
Outline of Final Research Achievements

The temperature inside single cells directly influences a broad range of biological processes and cellular functions. However, it remains unclear whether cells autonomously regulate their intracellular temperature against changes in the extracellular temperature. We measured the temperature intracellular temperature using two mechanistically independent intracellular thermometers. We found that the intracellular temperature is increased by about 3.0℃ in a manner dependent on Δ9-fatty acid desaturase DESAT1. The DESAT1-mediated increase of intracellular temperature was caused by the enhancement of F1Fo-ATPase-dependent mitochondrial respiration in the inner mitochondrial membrane. Cold exposure of S2 cells caused the enhanced production of unsaturated fatty acid-containing phospholipids in a DESAT1-dependent manner followed by the activation of mitochondrial respiration that is coupled with mitochondrial thermogenesis.

Academic Significance and Societal Importance of the Research Achievements

現在、地球温暖化に伴う生物種の大量絶滅、生息域の変化、感染症の拡大への対処が焦眉の課題として問われている。従来、環境温の変化が生命活動にいかなる影響を及ぼすかについての学術領域は、主に生態学や動物行動学をはじめとするマクロ生物学を軸として進められている。一方、現代の分子生物学の発展により生命活動を分子レベルで理解する基礎が築かれてきているが、生命活動に及ぼす温度の影響を分子レベルで体系的に理解する試みは未だわずかである。本研究では、細胞にも、動物と同様に、環境温の変動に応じて自律的に細胞内温度を制御する仕組みが存在すること、さらにその分子機構の一端を明らかにした点にその学術的意義がある。

Report

(7 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Products Report
  • Research Products

    (41 results)

All 2020 2019 2018 2017 2016 2015

All Journal Article (12 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 8 results) Presentation (28 results) (of which Int'l Joint Research: 6 results,  Invited: 26 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Changes in the physicochemical properties of fish cell membranes during cellular senescence2020

    • Author(s)
      Akifumi Shiomi, Kohjiro Nagao, Hisae Kasai, Yuji Hara, Masato Umeda
    • Journal Title

      BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY

      Volume: 84 Issue: 3 Pages: 583-593

    • DOI

      10.1080/09168451.2019.1695576

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure and Function of Δ9-Fatty Acid Desaturase2019

    • Author(s)
      Nagao, K., Murakami, A., Umeda, M.
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: 67 Issue: 4 Pages: 327-332

    • DOI

      10.1248/cpb.c18-01001

    • NAID

      130007621738

    • ISSN
      0009-2363, 1347-5223
    • Year and Date
      2019-04-01
    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Different mechanisms for selective transport of fatty acids using a single class of lipoprotein in Drosophila2019

    • Author(s)
      Naoya Matsuo, Kohjiro Nagao, Takuto Suito, Naoto Juni, Utako Kato, Yuji Hara, Masato Umeda
    • Journal Title

      Journal of Lipid Research

      Volume: 60 Issue: 7 Pages: 1199-1211

    • DOI

      10.1194/jlr.m090779

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation2018

    • Author(s)
      Tsuchiya Masaki、Hara Yuji、Okuda Masaki、Itoh Karin、Nishioka Ryotaro、Shiomi Akifumi、Nagao Kohjiro、Mori Masayuki、Mori Yasuo、Ikenouchi Junichi、Suzuki Ryo、Tanaka Motomu、Ohwada Tomohiko、Aoki Junken、Kanagawa Motoi、Toda Tatsushi、Nagata Yosuke、Matsuda Ryoichi、Takayama Yasunori、Tominaga Makoto、Umeda Masato
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 2049-2049

    • DOI

      10.1038/s41467-018-04436-w

    • NAID

      120006471161

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transport and Metabolism of Carotenoids in Drosophila melanogaster.2018

    • Author(s)
      Suito, T., Nagao, K., Umeda, M.
    • Journal Title

      Carotenoid Science

      Volume: 印刷中

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of omega-3 long-chain polyunsaturated fatty acid-rich triacylglycerols in an endemic goby, Gymnogobius isaza, from Lake Biwa, Japan2018

    • Author(s)
      Suito Takuto、Nagao Kohjiro、Hatano Masataka、Kohashi Kenichi、Tanabe Aiko、Ozaki Hiromichi、Kawamoto Jun、Kurihara Tatsuo、Mioka Tetsuo、Tanaka Kazuma、Hara Yuji、Umeda Masato
    • Journal Title

      The Journal of Biochemistry

      Volume: in press Issue: 2 Pages: 127-140

    • DOI

      10.1093/jb/mvy035

    • NAID

      40021635152

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pleckstrin homology domain of p210 BCR-ABL interacts with cardiolipin to regulate its mitochondrial translocation and subsequent mitophagy2018

    • Author(s)
      Shimasaki K, Watanabe-Takahashi M, Umeda M, Funamoto S, Saito Y, Noguchi N, Kumagai K, Hanada K, Tsukahara F, Maru Y, Shibata N, Naito M, Nishikawa K.
    • Journal Title

      Genes to Cells

      Volume: 23 Issue: 1 Pages: 22-34

    • DOI

      10.1111/gtc.12544

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An N-terminal di-proline motif is essential for fatty acid-dependent degradation of Δ9-desaturase inDrosophila2017

    • Author(s)
      Murakami Akira、Nagao Kohjiro、Juni Naoto、Hara Yuji、Umeda Masato
    • Journal Title

      Journal of Biological Chemistry

      Volume: 292 Issue: 49 Pages: 19976-19986

    • DOI

      10.1074/jbc.m117.801936

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ショウジョウバエを用いた体温調節行動の解析:温度受容体と共生細菌を介した制御2017

    • Author(s)
      水藤拓人・長尾耕治郎・梅田眞郷
    • Journal Title

      化学と生物

      Volume: 55 Pages: 803-809

    • NAID

      130007519790

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 生体膜におけるリン脂質ダイナミクスとその生物機能2016

    • Author(s)
      土谷正樹・原雄二・梅田眞郷
    • Journal Title

      膜

      Volume: 41 Pages: 196-201

    • NAID

      130005421182

    • Related Report
      2016 Annual Research Report
  • [Journal Article] ショウジョウバエのリン脂質輸送タンパク質-ユニークな形質膜のリン脂質の組成と分布2016

    • Author(s)
      長尾耕治郎・塩見晃史・梅田眞郷
    • Journal Title

      生体の化学

      Volume: 63 Pages: 247-251

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates2015

    • Author(s)
      Bhat HB, Ishitsuka R, Inaba T, Murate M, Abe M, Makino A, Kohyama-Koganeya A, Nagao K, Kurahashi A, Kishimoto T, Tahara M, Yamano A, Nagamune K, Hirabayashi Y, Juni N, Umeda M, Fujimori F, Nishibori K, Yamaji-Hasegawa A, Greimel P, Kobayashi T.
    • Journal Title

      FASEB J

      Volume: 29(9) Issue: 9 Pages: 3920-34

    • DOI

      10.1096/fj.15-272112

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 脂質ダイナミズムから生命現象を理解する2020

    • Author(s)
      梅田眞郷
    • Organizer
      リポクオリティ領域会議
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 脂質改変による冬眠の誘導2019

    • Author(s)
      梅田眞郷、原雄二、長尾耕治郎
    • Organizer
      理化学研究所BDR人工冬眠に向けての勉強会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 膜脂質を介する細胞内温度制御の分子機構2019

    • Author(s)
      梅田眞郷、村上光、長尾耕治郎
    • Organizer
      第71回日本細胞生物学会シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 脂質の分子運動から生命現象を探る2019

    • Author(s)
      梅田眞郷
    • Organizer
      生化学若い研究者の会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 膜脂質を介する細胞内温度の制御機構2019

    • Author(s)
      梅田眞郷、村上光、長尾耕治郎
    • Organizer
      第57回日本生物物理学会年会シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 昆虫から学ぶ骨格筋細胞の膜と代謝2019

    • Author(s)
      梅田眞郷
    • Organizer
      第7回若手骨格筋研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 生体膜の分子機構2019

    • Author(s)
      梅田眞郷
    • Organizer
      兵庫医科大学特別講演
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 生物と温度2019

    • Author(s)
      梅田眞郷
    • Organizer
      和敬論談会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Organization and deformability of insect cell membrane.2019

    • Author(s)
      Masato Umeda
    • Organizer
      The 16th International Membrane Research Forum
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 脂質ダイナミクスの生物機能2018

    • Author(s)
      梅田眞郷
    • Organizer
      第13回スフィンゴテラピィ研究会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Phospholipid flippase acts as a molecular switch for ion channel activation.2018

    • Author(s)
      Masato Umeda
    • Organizer
      Membrane Lipid Transporter Symposium 2018-Flippases, Floppases and Scramblases
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Phospholipid flip-flop as a molecular switch for ion channel activation.2018

    • Author(s)
      Masato Umeda
    • Organizer
      2nd Japan-Korea Lipid Joint Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Phospholipid flip-flop as a molecular switch for ion channel activation.2018

    • Author(s)
      Masato Umeda
    • Organizer
      The 23rd International Symposium on Plant Lipids
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 膜リン脂質のフリップフロップ・スイッチによるイオンチャネルの制御2017

    • Author(s)
      梅田眞郷
    • Organizer
      老化過程における細胞膜のリン脂質非対称性の生理機能と病態に関するセミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 昆虫から学ぶ2017

    • Author(s)
      梅田眞郷
    • Organizer
      新潟薬科大学薬学総合セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 昆虫から学ぶ脂質の機能と代謝2017

    • Author(s)
      梅田眞郷
    • Organizer
      第31回カロテノイド研究談話会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Remarkable deformability of insect cell is supported by constitutively active phospholipid scramblase.2017

    • Author(s)
      Masato Umeda, Akifumi Shiomi, Kohjiro Nagao
    • Organizer
      Molecular & Cellular Biology of Gordon Research Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Dietary response governing thermoregulatory behavior2016

    • Author(s)
      Masato Umeda, Takuto Suito, Naoto Juni, Kojiro Nagao
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 腸内細菌を介する行動性体温調節の分子機構2016

    • Author(s)
      梅田眞郷、水藤拓人、従二直人、長尾耕治郎
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター(仙台)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 体温はいかにして決まるのか?2016

    • Author(s)
      梅田眞郷
    • Organizer
      HiHA第7回Workshop
    • Place of Presentation
      広島大学(広島)
    • Year and Date
      2016-09-23
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 温度センシングとエネルギー代謝2016

    • Author(s)
      梅田眞郷
    • Organizer
      第68回日本細胞生化学会大会
    • Place of Presentation
      京都テルサ(京都)
    • Year and Date
      2016-06-15
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 膜脂質ダイナミクスを介する細胞機能の制御機構2016

    • Author(s)
      梅田真郷
    • Organizer
      日本膜学会「第38年会」
    • Place of Presentation
      早稲田大学西早稲田キャンパス(東京)
    • Year and Date
      2016-05-10
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 膜脂質ダイナミクスを介する細胞機能の制御機構2016

    • Author(s)
      梅田真郷
    • Organizer
      日本膜学会「第38年会」
    • Place of Presentation
      早稲田大学(東京)
    • Year and Date
      2016-05-10
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 質を介するエネルギー代謝と温度調節行動の制御機構2016

    • Author(s)
      梅田真郷
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      札幌コンベンションセンター(札幌)
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 生体膜脂質と温度感受性システム2016

    • Author(s)
      梅田真郷
    • Organizer
      第36回白金シンポジウム
    • Place of Presentation
      北里大学薬学部(東京)
    • Year and Date
      2016-02-27
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 生体膜におけるリン脂質分子の運動と機能2015

    • Author(s)
      梅田真郷
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(金沢)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 呼吸とエネルギー代謝:昆虫の秘密から学ぶ2015

    • Author(s)
      梅田真郷
    • Organizer
      第19回酵素ダイナミクス研究会
    • Place of Presentation
      東京女子医科大学(東京)
    • Year and Date
      2015-09-12
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] symmetric transbilayer distribution of membrane phospholipids is disrupted by constitutive activation of phospholipid scramblase in insect cells.2015

    • Author(s)
      Masato Umeda
    • Organizer
      Lipids, Molecular & Cellular Biology of Gordon Research Conference
    • Place of Presentation
      Waterville Valley(USA)
    • Year and Date
      2015-07-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] エイコサンペンタエン酸を生産する微生物2020

    • Inventor(s)
      梅田 眞郷, 従二 直人, 長尾 耕治郎
    • Industrial Property Rights Holder
      国立大学法人京都大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2020-123809
    • Filing Date
      2020
    • Related Report
      Products Report

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Published: 2015-07-02   Modified: 2022-11-04  

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