| Budget Amount *help |
¥95,030,000 (Direct Cost: ¥73,100,000、Indirect Cost: ¥21,930,000)
Fiscal Year 2019: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2018: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2017: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2015: ¥28,470,000 (Direct Cost: ¥21,900,000、Indirect Cost: ¥6,570,000)
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| Outline of Final Research Achievements |
This study developed a centrifugal fluorescence microscope system that can observe the gravity response of cells in real time. In this way, we succeeded in live imaging of morphological changes of cells due to gravitational stimulation by rotation for several tens of minutes.
We also discovered a new mechanism for the gravity sensing of cells. In space, the bone density of astronauts decreases. In agreement with this, simulated microgravity acts as a suppressive factor in bone differentiation of mesenchymal stem cells. Our results suggest that the activity of the mechanosensitive ion channel TRPV4 is reduced in the simulated microgravity environment, and the activity of the protein kinase C is subsequently reduced to suppress the nuclear translocation of the transcriptional cofactor YAP. In addition, we have also developed a statistical method for uniformly analyzing gene expression levels among different species.
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