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3D architecture of mitotic chromosomes

Planned Research

Project AreaChromosome Orchestration System
Project/Area Number 15H05971
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Hirano Tatsuya  国立研究開発法人理化学研究所, 開拓研究本部, 主任研究員 (50212171)

Co-Investigator(Kenkyū-buntansha) 大杉 美穂  東京大学, 大学院総合文化研究科, 教授 (00332586)
Project Period (FY) 2015-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥157,950,000 (Direct Cost: ¥121,500,000、Indirect Cost: ¥36,450,000)
Fiscal Year 2019: ¥29,640,000 (Direct Cost: ¥22,800,000、Indirect Cost: ¥6,840,000)
Fiscal Year 2018: ¥29,770,000 (Direct Cost: ¥22,900,000、Indirect Cost: ¥6,870,000)
Fiscal Year 2017: ¥29,770,000 (Direct Cost: ¥22,900,000、Indirect Cost: ¥6,870,000)
Fiscal Year 2016: ¥29,640,000 (Direct Cost: ¥22,800,000、Indirect Cost: ¥6,840,000)
Fiscal Year 2015: ¥39,130,000 (Direct Cost: ¥30,100,000、Indirect Cost: ¥9,030,000)
Keywords染色体構築 / 細胞周期 / コンデンシン / 細胞生物学 / 生化学 / ゲノム / 染色体 / 細胞分裂
Outline of Final Research Achievements

We established a novel in-vitro system for studying mitotic chromosome assembly, in which mouse sperm nuclei were incubated with frog egg extracts. By taking full advantage of this unique system, it was discovered that mitotic chromosome-like structures can be constructed, in a manner dependent of condensins I and II, even under the condition where nucleosome assembly in completely blocked. Moreover, we uncovered a molecular mechanism that helps delay pronuclei formation in mouse eggs after fertilization.

Academic Significance and Societal Importance of the Research Achievements

「ヌクレオソームを持たない分裂期染色体」の構築は、従来の教科書を書き換える画期的な発見として世界的に大きな評価を得た。この発見を可能にした新規実験系の確立にも大きな意義がある。

Report

(6 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (48 results)

All 2019 2018 2017 2016 2015 Other

All Journal Article (13 results) (of which Peer Reviewed: 13 results,  Open Access: 2 results,  Acknowledgement Compliant: 4 results) Presentation (31 results) (of which Int'l Joint Research: 14 results,  Invited: 26 results) Remarks (3 results) Funded Workshop (1 results)

  • [Journal Article] Molecular dynamics simulations of condensin-mediated mitotic chromosome assembly2019

    • Author(s)
      Yuji Sakai, Tatsuya Hirano, Masashi Tachikawa
    • Journal Title

      Methods in Molecular Biology

      Volume: 2004 Pages: 319-334

    • DOI

      10.1007/978-1-4939-9520-2_22

    • ISBN
      9781493995196, 9781493995202
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis of HEAT‐kleisin interactions in the human condensin I subcomplex2019

    • Author(s)
      Hara Kodai、Kinoshita Kazuhisa、Migita Tomoko、Murakami Kei、Shimizu Kenichiro、Takeuchi Kozo、Hirano Tatsuya、Hashimoto Hiroshi
    • Journal Title

      EMBO reports

      Volume: - Issue: 5

    • DOI

      10.15252/embr.201847183

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dynamic organization of mitotic chromosomes.2017

    • Author(s)
      Kazuhisa Kinoshita and Tatsuya Hirano
    • Journal Title

      Current Opinion in Cell Biology

      Volume: 46 Pages: 46-53

    • DOI

      10.1016/j.ceb.2017.01.006

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Overall shapes of the SMC-ScpAB complex are determined by balance between constraint and relaxation of its structural parts2017

    • Author(s)
      Kamada K, Suetsugu M, Takada H, Miyata M, Hirano T
    • Journal Title

      Structure

      Volume: 25 Issue: 4 Pages: 603-16

    • DOI

      10.1016/j.str.2017.02.008

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mitotic chromosome assembly despite nucleosome depletion in Xenopus egg extracts.2017

    • Author(s)
      Shintomi K, Inoue F, Watanabe H, Ohsumi K, Ohsugi M, Hirano T.
    • Journal Title

      Science

      Volume: 356 Issue: 6344 Pages: 1284-1287

    • DOI

      10.1126/science.aam9702

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Capturing condensin in chromosomes2017

    • Author(s)
      Hirano T.
    • Journal Title

      Nat Genet

      Volume: 49 Issue: 10 Pages: 1419-1420

    • DOI

      10.1038/ng.3962

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hot debate in hot springs: Report on the second international meeting on SMC proteins.2017

    • Author(s)
      Hirano T, Nishiyama T, Shirahige K.
    • Journal Title

      Genes Cells.

      Volume: 22 Issue: 11 Pages: 934-938

    • DOI

      10.1111/gtc.12539

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mitotic Chromosome Assembly In Vitro: Functional Cross Talk between Nucleosomes and Condensins2017

    • Author(s)
      Shintomi K, Hirano T.
    • Journal Title

      Cold Spring Harb Symp Quant Biol.

      Volume: - Pages: 033639-033639

    • DOI

      10.1101/sqb.2017.82.033639

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HEAT repeats - versatile arrays of amphiphilic helices working in crowded environments?2016

    • Author(s)
      S.H. Yoshimura and T. Hirano
    • Journal Title

      J. Cell Sci.

      Volume: 129 Issue: 21 Pages: 3963-3970

    • DOI

      10.1242/jcs.185710

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The microtubule-binding and coiled-coil domains of Kid are required for turning off the polar ejection force at anaphase2016

    • Author(s)
      Shou Soeda, Kaori Yamada-Nomoto, Miho Ohsugi
    • Journal Title

      Journal of Cell Science

      Volume: 129 Issue: 19 Pages: 3609-3619

    • DOI

      10.1242/jcs.189969

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Condensin-based chromosome organization from bacteria to vertebrates2016

    • Author(s)
      Hirano, T.
    • Journal Title

      Cell

      Volume: 164 Issue: 5 Pages: 847-857

    • DOI

      10.1016/j.cell.2016.01.033

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Reconstitution of mitotic chromatids with a minimum set of purified factors2015

    • Author(s)
      Keishi Shintomi, Tatsuro S. Takahashi, and Tatsuya Hirano
    • Journal Title

      Nature Cell Biology

      Volume: 17 Issue: 8 Pages: 1014-1023

    • DOI

      10.1038/ncb3187

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Balancing Acts of Two HEAT Subunits of Condensin I Support Dynamic Assembly of Chromosome Axes2015

    • Author(s)
      Kazuhisa Kinoshita, Tetsuya J. Kobayashi and Tatsuya Hirano
    • Journal Title

      Developmental Cell

      Volume: 33 Issue: 1 Pages: 94-106

    • DOI

      10.1016/j.devcel.2015.01.034

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] Condensin-based chromosome organization: new insights from in vitro assays2019

    • Author(s)
      T. Hirano
    • Organizer
      ASCB | EMBO 2019 meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The two faces of condensin I2019

    • Author(s)
      T. Hirano
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] The two faces of condensin I2019

    • Author(s)
      T. Hirano
    • Organizer
      EMBO Workshop on “SMC proteins”
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Making a chromosome from scratch: a powerful approach to dissecting mitotic functions of topoisomerase II alpha2019

    • Author(s)
      K. Shintomi and T. Hirano
    • Organizer
      EMBO Workshop on "DNA topology and topoisomerase in genome dynamics"
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Making a chromosome from scratch: a powerful approach to dissecting mitotic functions of topoisomerase II alpha2019

    • Author(s)
      K. Shintomi and T. Hirano
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Condensin-based chromosome organization: new insights from cell-free extracts2019

    • Author(s)
      T. Hirano
    • Organizer
      Gordon Research Conference on “Chromosome Dynamics”
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 染色体再構成によって見えてきたトポⅡの役割2019

    • Author(s)
      新冨圭史
    • Organizer
      遺伝研研究会~クロマチン・細胞核の形成とダイナミクスによるゲノム制御
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Condensin-based chromosome organization2018

    • Author(s)
      Hirano, T
    • Organizer
      Vienna Biocenter (Institutional seminar)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Condensin-based chromosome organization2018

    • Author(s)
      Hirano, T
    • Organizer
      EMBL (Institutional seminar)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Condensin-based chromosome organization2017

    • Author(s)
      Hirano, T
    • Organizer
      Keystone Symposium on “Genomic Instability and DNA Repair/DNA Replication and Recombination”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Condensin-based chromosome organization2017

    • Author(s)
      Hirano, T
    • Organizer
      Gordon Research Conference “Chromosome Dynamics”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 学際的視点からみる染色体構築学の現状とこれから2017

    • Author(s)
      平野達也
    • Organizer
      ConBio2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Mitotic chromosome assembly in vitro: functional collaboration between condensins and nucleosomes2017

    • Author(s)
      Shintomi, K. and Hirano, T.
    • Organizer
      The 82nd Cold Spring Harbor Symposium on Quantitative Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Functional crosstalk between condensins and nucleosomes during mitotic chromosome assembly2017

    • Author(s)
      Shintomi, K., Inoue, F., Watanabe, H., Ohsumi, K., Ohsugi, M. and Hirano, T.
    • Organizer
      The 2nd meeting on SMC proteins: chromosomal organizers from bacteria to human
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mitotic chromosome assembly in vitro: functional collaboration between condensins and nucleosomes2017

    • Author(s)
      Shintomi, K. and Hirano, T.
    • Organizer
      2017年度生命科学系学会合同年次大会 (ConBio2017)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 分裂期染色体構築におけるヌクレオソームの役割を再考する2017

    • Author(s)
      新冨圭史、井上玄志、渡邉大士、大隅圭太、大杉美穂、平野達也
    • Organizer
      第35回染色体ワークショップ・第16回核ダイナミクス研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 受精開始から前核形成までの時間短縮による発生異常2017

    • Author(s)
      添田翔、大杉美穂
    • Organizer
      第35回日本受精着床学会総会・学術講演会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Nuclear scaling in mouse zygotes2017

    • Author(s)
      渡辺大士、大杉美穂
    • Organizer
      2017年度生命科学系学会合同年次大会 (ConBio2017)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Condensin-based chromosome organization2017

    • Author(s)
      Hirano T
    • Organizer
      The Francis Crick Institute (Flagship Lecture)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 分裂期染色体構築におけるヌクレオソームの役割2017

    • Author(s)
      新冨圭史、井上玄志、大隅圭太、大杉美穂、平野達也
    • Organizer
      第34回染色体ワークショップ・第15回核ダイナミクス研究会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Reconstitution of mitotic chromatids in vitro2016

    • Author(s)
      Hirano T
    • Organizer
      ASCB Annual Meeting, Special Interest Subgroup “Bottom-up Cell Biology”
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Condensin-based chromosome organization2016

    • Author(s)
      Hirano T
    • Organizer
      CECB Symposium "Nuclear Structure, Genome Integrity and Cancer"
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Condensin-based chromosome organization2016

    • Author(s)
      Hirano T
    • Organizer
      Workshop on the “Molecular and Physical Biology of Chromosomes”
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Condensin-based chromosome organization2016

    • Author(s)
      Hirano T
    • Organizer
      Gordon Research Conference “Chromatin Structure and Function”
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] コンデンシンによる染色体構築の分子基盤2016

    • Author(s)
      平野達也
    • Organizer
      第89回日本生化学会大会
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 分裂期染色体の構築原理:試験管内再構成系からのアプローチ2016

    • Author(s)
      平野達也
    • Organizer
      北海道大学セミナー
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 染色体構築入門:コンデンシンの発見からその生体内機能まで2016

    • Author(s)
      平野達也
    • Organizer
      北海道大学大学院・分子細胞生物学特論
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Pronuclear Size Scaling in Mouse Zygotes2016

    • Author(s)
      Watanabe, H, Ohsugi, M
    • Organizer
      The 10th 3R Symposium
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pronuclear Size Scaling in Mouse Zygotes2016

    • Author(s)
      Watanabe, H, Ohsugi, M
    • Organizer
      The 2016 ASCB annual meeting
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 分裂期染色体の構築原理2015

    • Author(s)
      平野達也
    • Organizer
      第38回日本分子生物学会年会・第88回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 分裂期染色分体の試験管内再構成2015

    • Author(s)
      平野達也
    • Organizer
      第67回日本細胞生物学会
    • Place of Presentation
      東京
    • Year and Date
      2015-06-30
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Remarks] New info about how chromosomes form

    • URL

      http://itaintmagic.riken.jp/science-lite/new-chromosomes-info/

    • Related Report
      2017 Annual Research Report
  • [Remarks] Making do with less

    • URL

      http://www.riken.jp/en/research/rikenresearch/highlights/20170016/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 分裂期染色体をつくるためにヌクレオソームは本当に必須なのか?

    • URL

      http://first.lifesciencedb.jp/archives/16664

    • Related Report
      2017 Annual Research Report
  • [Funded Workshop] SMC Proteins: chromosomal organizers from bacteria to human2017

    • Related Report
      2017 Annual Research Report

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Published: 2015-07-02   Modified: 2022-11-04  

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