| Budget Amount *help |
¥117,520,000 (Direct Cost: ¥90,400,000、Indirect Cost: ¥27,120,000)
Fiscal Year 2020: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
Fiscal Year 2019: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
Fiscal Year 2018: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
Fiscal Year 2017: ¥22,750,000 (Direct Cost: ¥17,500,000、Indirect Cost: ¥5,250,000)
Fiscal Year 2016: ¥29,770,000 (Direct Cost: ¥22,900,000、Indirect Cost: ¥6,870,000)
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| Outline of Final Research Achievements |
Our previous studies have shown that the binding of ApoE or Erns to the membrane of viral particles is essential for the formation of infectious particles of hepatitis C virus (HCV) and pestiviruses. We have established a system of trans-complementation, and identified the I273H mutation. Although the virus carrying this mutation is capable of replicating the genome, infectious particle formation was inhibited, sugggesting that the NS1 protein is important for virus particle formation. Analysis of the purified NS1 protein with I273 mutation also revealed that this mutation is important for membrane binding. Viruses with this I273 mutation cannot form infectious viral particles, but the exogenous expression of ApoE and Erns compensated for their function. Considering these results, viruses belonging to the Flaviviridae family have a similar mechanism in particle formation. It is possible that viral proteins and host factors have come to be used in the process of evolution.
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