| Budget Amount *help |
¥117,520,000 (Direct Cost: ¥90,400,000、Indirect Cost: ¥27,120,000)
Fiscal Year 2020: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
Fiscal Year 2019: ¥22,750,000 (Direct Cost: ¥17,500,000、Indirect Cost: ¥5,250,000)
Fiscal Year 2018: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
Fiscal Year 2017: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
Fiscal Year 2016: ¥29,770,000 (Direct Cost: ¥22,900,000、Indirect Cost: ¥6,870,000)
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| Outline of Final Research Achievements |
The objective of this project is to establish two neo-lifestyles of fungal viruses and explore their generality and ecological significance. First, we have proved the yadokari/ yadonushi nature in Japanese strains of Rosellinia necatrix and revealed its molecular basis. Capsidless yadokari virus 1 (YkV1) highjacks the capsid of yadnushi virus 1 (YnV1) to replicate in the heterocapsid using its own RNA-dependent RNA polymerase, while in return YkV1 trans-enhances the replication of YnV1. For the hadaka nature, we have shown that hadaka virus 1 (HadV1) from a Pakistani strain of Fusarium oxysporum exists as a capsidless naked form at least in mycelial homogenates that is accessible by RNase A and unable to be pelleted by untracentrifugation. While the yadonushi/yadokari nature has been observed not only foreign fungal strains but also in plants,the hadaka nature has also been detected in other filamentous fungi, suggesting suggesting the generality of the two viral neo-lifestyles.
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