| Budget Amount *help |
¥72,020,000 (Direct Cost: ¥55,400,000、Indirect Cost: ¥16,620,000)
Fiscal Year 2020: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2019: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2018: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2017: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2016: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
|
|---|
| Outline of Final Research Achievements |
Deciphering the biosynthesis of fungal natural products, especially focusing on polyketides, was examined by through heterologous expression and in vitro/in vivo functional analysis of biosynthetic enzymes. Of particular notable is that missing stereochemical course of the PK processings catalyzed by fungal polyketide synthase (PKS) was elucidated. Bioinformatics analysis of PKS supports the stereochemical course. During the experiments, we established Hot spot-Knock-in method selectively to introduce the biosynthetic genes into the high expression loci (Hot spot). This method is applied to reconstitute the biosynthetic machinery of fungal natural products and to check the splicing of the biosynthetic genes derived from Basidiomycetes fungi.
|
