| Budget Amount *help |
¥72,020,000 (Direct Cost: ¥55,400,000、Indirect Cost: ¥16,620,000)
Fiscal Year 2020: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2019: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2018: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2017: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2016: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
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| Outline of Final Research Achievements |
Mix-and-match of biosynthetic genes, followed by extensive directed evolution round has enabled biosynthesis of various non-natural or even extra-natural compounds. To improve the titers of these artificial pathways, they should be nicely embedded into the metabolic networks of host organisms. In this project, (1) we tried to co-evolve exogenous terpenoid pathway and endogenous precursor pathway to improve both to the unprecedented level. Multi-round evolution isolated terpene synthase mutants with improved cellular activity, alongside with greatly improved precursor suppliers. Also, (2) we developed technology to create enzyme-based biosensors, which enable screening-based pathway design for various natural/unnatural compounds. Based on the evolutionary platform for sensors and enzymes, we have established pathways toward more than 50 novel compounds with unique biophysical/ photochemical properties.
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