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Development and precise functional analysis of innovative biosynthetic machinery to create complex skeletons

Planned Research

Project AreaCreation of Complex Functional Molecules by Rational Redesign of Biosynthetic Machineries
Project/Area Number 16H06453
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionThe University of Tokyo

Principal Investigator

Kuzuyama Tomohisa  東京大学, 大学院農学生命科学研究科(農学部), 教授 (30280952)

Co-Investigator(Kenkyū-buntansha) 白石 太郎  東京大学, 生物生産工学研究センター, 助教 (40734603)
Project Period (FY) 2016-06-30 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥72,020,000 (Direct Cost: ¥55,400,000、Indirect Cost: ¥16,620,000)
Fiscal Year 2020: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2019: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2018: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2017: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2016: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Keywords生物活性物質 / 天然化合物 / 遺伝子 / 酵素 / 生合成 / 反応機構 / 電子環状反応 / 生合成マシナリー / 放線菌 / ヌクレオシド / ポリケチド / Biosynthesis / nucleoside antibiotic / carbazole / terpene / reaction mechanism / 生合成遺伝子 / 天然物 / テルペノイド / ホスホン酸 / ポリケタイド / 生体触媒 / 結晶構造解析
Outline of Final Research Achievements

In this research project, through the discovery of innovative biosynthetic enzymes that create complex chemical structures of natural products and the precise analysis of their functions, we have been able to reveal a number of new mechanisms by which nature creates structural diversity in natural products. Some of these results should be included in textbooks of biochemistry and natural product chemistry in the future. All of these results were achieved by combining the methods of molecular biology, biochemistry, structural biology, organic chemistry, and computational science, and we are confident that we have contributed to the establishment of new theories in natural product chemistry.

Academic Significance and Societal Importance of the Research Achievements

我々は、多くの生物のゲノム情報が容易に入手可能となった現在、様々な有用な天然化合物の生合成遺伝子を取得し、その生合成系を異種微生物の中に再構築することで狙った付加価値の高い化合物を生体触媒(酵素)の力を借りて生産する方法を、本研究課題を通して確立した。本研究成果は、爆発的に蓄積し続ける未利用資源とも言うべきゲノム情報から未知の有益な配列情報を効率的に抽出し、生物合成と合成化学の手法で生産することで未踏の有用物質を人類の生活向上への活用につなげるための必須の方法となるであろう。

Report

(6 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (89 results)

All 2021 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (5 results) Journal Article (22 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 21 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (52 results) (of which Int'l Joint Research: 20 results,  Invited: 18 results) Remarks (10 results)

  • [Int'l Joint Research] University of Texas at Austin(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Bonn(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] テキサス大学(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ウイーン大学(オーストリア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] The University of Texas at Austin(米国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Reconstitution of a Highly Reducing Type II PKS System Reveals 6π-Electrocyclization Is Required for o-Dialkylbenzene Biosynthesis2021

    • Author(s)
      Zhang J, Yuzawa S, Thong WL, Shinada T, Nishiyama M, Kuzuyama T.
    • Journal Title

      J Am Chem Soc

      Volume: 143 Issue: 7 Pages: 2962-2969

    • DOI

      10.1021/jacs.0c13378

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthetic pathways and enzymes involved in the production of phosphonic acid natural products2021

    • Author(s)
      Shiraishi T, Kuzuyama T.
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 85 Issue: 1 Pages: 42-52

    • DOI

      10.1093/bbb/zbaa052

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Recent Advances in the Biosynthesis of Carbazoles Produced by Actinomycetes2020

    • Author(s)
      Kobayashi M, Kuzuyama T.
    • Journal Title

      Biomolecules

      Volume: 10 Issue: 8 Pages: 1147-1147

    • DOI

      10.3390/biom10081147

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Amipurimycin and Miharamycin Biosynthetic Gene Clusters: Unraveling the Origins of 2-Aminopurinyl Peptidyl Nucleoside Antibiotics2019

    • Author(s)
      Romo AJ, Shiraishi T, Ikeuchi H, Lin GM, Geng Y, Lee YH, Liem PH, Ma T, Ogasawara Y, Shin-ya K, Nishiyama M, Kuzuyama T, Liu HW.
    • Journal Title

      J Am Chem Soc

      Volume: 141 Issue: 36 Pages: 14152-14159

    • DOI

      10.1021/jacs.9b03021

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] An Unprecedented Cyclization Mechanism in the Biosynthesis of Carbazole Alkaloids in Streptomyces2019

    • Author(s)
      Kobayashi M, Tomita T, Shin-ya K, Nishiyama M, Kuzuyama T.
    • Journal Title

      Angew Chem Int Ed Engl

      Volume: 58 Issue: 38 Pages: 13349-13353

    • DOI

      10.1002/anie.201906864

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An Unusual Skeletal Rearrangement in the Biosynthesis of the Sesquiterpene Trichobrasilenol from Trichoderma2019

    • Author(s)
      Murai K, Lauterbach L, Teramoto K, Quan Z, Barra L, Yamamoto T, Nonaka K, Shiomi K, Nishiyama M, Kuzuyama T, Dickschat JS.
    • Journal Title

      Angew Chem Int Ed Engl

      Volume: 58 Issue: 42 Pages: 15046-15050

    • DOI

      10.1002/anie.201907964

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Recent advances in the biosynthesis of nucleoside antibiotics2019

    • Author(s)
      Shiraishi T, Kuzuyama T.
    • Journal Title

      J Antibiot

      Volume: 72 Issue: 12 Pages: 913-923

    • DOI

      10.1038/s41429-019-0236-2

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 新奇骨格を創成する革新的生合成マシナリーの開拓2019

    • Author(s)
      白石 太郎、葛山 智久
    • Journal Title

      Farumashia

      Volume: 55 Issue: 7 Pages: 679-683

    • DOI

      10.14894/faruawpsj.55.7_679

    • NAID

      130007671598

    • ISSN
      0014-8601, 2189-7026
    • Related Report
      2019 Annual Research Report
  • [Journal Article] Biosynthesis of the uridine-derived nucleoside antibiotic A-94964: Identification and characterization of the biosynthetic gene cluster provide insight into the biosynthetic pathway2019

    • Author(s)
      Shiraishi, T., Nishiyama, M., Kuzuyama T.
    • Journal Title

      Org. Biomol. Chem.

      Volume: 17 Issue: 3 Pages: 461-466

    • DOI

      10.1039/c8ob02765j

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural and mechanistic insight into terpene synthases that catalyze the irregular non-head-to-tail coupling of prenyl substrates2019

    • Author(s)
      Kobayashi, M., Kuzuyama, T.
    • Journal Title

      Chembiochem

      Volume: 20 Issue: 1 Pages: 29-33

    • DOI

      10.1002/cbic.201800510

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Discovery of an antibacterial isoindolinone-containing tetracyclic polyketide by cryptic gene activation and characterization of its biosynthetic gene cluster2018

    • Author(s)
      Thong, W. L., Shin-ya, K., Nishiyama, M., Kuzuyama, T.
    • Journal Title

      ACS Chem. Biol.

      Volume: 13 Issue: 9 Pages: 2615-2622

    • DOI

      10.1021/acschembio.8b00553

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biochemical and structural analysis of FomD that catalyzes the hydrolysis of cytidylyl (S)-2-hydroxypropylphosphonate in fosfomycin biosynthesis2018

    • Author(s)
      Sato, S., Miyanaga, A., Kim, S. Y., Kuzuyama, T., Kudo, F., Eguchi, T.
    • Journal Title

      Biochemistry

      Volume: 57 Issue: 32 Pages: 4858-4866

    • DOI

      10.1021/acs.biochem.8b00690

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Discovery of Unprecedented Hydrazine-Forming Machinery in Bacteria2018

    • Author(s)
      Matsuda Kenichi、Tomita Takeo、Shin-ya Kazuo、Wakimoto Toshiyuki、Kuzuyama Tomohisa、Nishiyama Makoto
    • Journal Title

      Journal of the American Chemical Society

      Volume: 140 Issue: 29 Pages: 9083-9086

    • DOI

      10.1021/jacs.8b05354

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] C-Methylation Catalyzed by Fom3, a Cobalamin-Dependent Radical S-adenosyl-l-methionine Enzyme in Fosfomycin Biosynthesis, Proceeds with Inversion of Configuration2018

    • Author(s)
      Sato, S., Kudo, F., Kuzuyama, T., Hammerschmidt, F., Eguchi, T.
    • Journal Title

      Biochemistry

      Volume: 57 Issue: 33 Pages: 4963-4966

    • DOI

      10.1021/acs.biochem.8b00614

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Biosynthetic studies on terpenoids produced by Streptomyces2017

    • Author(s)
      Kuzuyama, T.
    • Journal Title

      J. Antibiot.

      Volume: 印刷中 Issue: 7 Pages: 811-818

    • DOI

      10.1038/ja.2017.12

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Characterization of bafilomycin biosynthesis in Kitasatospora setae KM-6054 and comparative analysis of gene clusters in Actinomycetales microorganisms2017

    • Author(s)
      Nara, A., Hashimoto, T., Komatsu, M., Nishiyama, M., Kuzuyama, T., Ikeda, H.
    • Journal Title

      J. Antibiot.

      Volume: 印刷中 Issue: 5 Pages: 616-624

    • DOI

      10.1038/ja.2017.33

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural Insights into the CotB2-Catalyzed Cyclization of Geranylgeranyl Diphosphate to the Diterpene Cyclooctat-9-en-7-ol2017

    • Author(s)
      Takeo Tomita, Seung-Young Kim, Kazuya Teramoto, Ayuko Meguro, Taro Ozaki, Ayako Yoshida, Yudai Motoyoshi, Naoki Mori, Ken Ishigami, Hidenori Watanabe, Makoto Nishiyama, Tomohisa Kuzuyama
    • Journal Title

      ACS Chem. Biol.

      Volume: 12 Issue: 6 Pages: 1621-1628

    • DOI

      10.1021/acschembio.7b00154

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthetic Origin of the Hydroxamic Acid Moiety of Trichostatin A: Identification of Unprecedented Enzymatic Machinery Involved in Hydroxylamine Transfer2017

    • Author(s)
      Kei Kudo, Taro Ozaki, Kazuo Shin-ya, Makoto Nishiyama, Tomohisa Kuzuyama
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 139 Issue: 20 Pages: 6799-6802

    • DOI

      10.1021/jacs.7b02071

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Methylcobalamin-Dependent Radical SAM C-Methyltransferase Fom3 Recognizes Cytidylyl-2-hydroxyethylphosphonate and Catalyzes the Nonstereoselective C-Methylation in Fosfomycin Biosynthesis2017

    • Author(s)
      Sato Shusuke、Kudo Fumitaka、Kim Seung-Young、Kuzuyama Tomohisa、Eguchi Tadashi
    • Journal Title

      Biochemistry

      Volume: 56 Issue: 28 Pages: 3519-3522

    • DOI

      10.1021/acs.biochem.7b00472

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fosfomycin Biosynthesis via Transient Cytidylylation of 2-Hydroxyethylphosphonate by the Bifunctional Fom1 Enzyme2017

    • Author(s)
      Su-Hee Cho, Seung-Young Kim, Takeo Tomita, Taro Shiraishi, Jin-Soo Park, Shusuke Sato, Fumitaka Kudo, Tadashi Eguchi, Nobutaka Funa, Makoto Nishiyama and Tomohisa Kuzuyama
    • Journal Title

      ACS Chem. Biol.

      Volume: 12 Pages: 2209-2215

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure and Mechanism of the Monoterpene Cyclolavandulyl Diphosphate Synthase that Catalyzes Consecutive Condensation and Cyclization2017

    • Author(s)
      Takeo Tomita, Masaya Kobayashi, Yuma Karita, Yoko Yasuno, Tetsuro Shinada, Makoto Nishiyama, Tomohisa Kuzuyama
    • Journal Title

      Angew. Chem. Int Ed.

      Volume: 56 Issue: 47 Pages: 14913-14917

    • DOI

      10.1002/anie.201708474

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanistic insights into Diels-Alder reactions in natural product biosynthesis2017

    • Author(s)
      Hashimoto, T., Kuzuyama, T.
    • Journal Title

      Curr. Opin. Chem. Biol.

      Volume: 35 Pages: 117-123

    • DOI

      10.1016/j.cbpa.2016.09.015

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] スクアレン合成酵素に似た放線菌由来芳香族プレニル基転移酵素の構造学的研究2021

    • Author(s)
      永田 隆平、末宗 周憲、山本 将輝、小林 正弥、品田 哲郎、西山 真、永野 真吾、葛山 智久
    • Organizer
      日本農芸化学会2021年度大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Biosynthesis of the nucleoside-derived natural product angustmycin2021

    • Author(s)
      Jiaqi XIA, Taro SHIRAISHI, Tomohisa KUZUYAMA
    • Organizer
      日本農芸化学会2021年度大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 生合成の in vitro 再構成系を利用した新規 trichostatin 類縁体の創生2021

    • Author(s)
      松田 悠太郎、湯澤 賢、青山 美和子、大澤 宏祐、 吉田 将人、土井 隆行、新家 一男、西山 真、葛山 智久
    • Organizer
      日本農芸化学会2021年度大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 複雑骨格を構築する生合成マシナリーの機能予知2021

    • Author(s)
      葛山 智久
    • Organizer
      日本農芸化学会2021年度大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Understanding Biochemical Mechanism in Natural Product Biosynthesis2019

    • Author(s)
      Tomohisa Kuzuyama
    • Organizer
      Genentech Lecture
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 生合成再設計を指向した天然化合物骨格形成機構の解明2019

    • Author(s)
      葛山智久
    • Organizer
      微生物ウィークシンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 微生物の多様なイソプレノイド生合成機構2019

    • Author(s)
      葛山智久
    • Organizer
      琉球大学熱帯生物圏研究センター第5回 TBRCセミナー
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 複雑骨格を創成する革新的生合成マシナリーの開拓と精密機能解析2019

    • Author(s)
      葛山智久
    • Organizer
      新学術領域生合成リデザイン第7回公開シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Understanding Biochemical Mechanism in Terpenoid Biosynthesis2019

    • Author(s)
      Tomohisa Kuzuyama
    • Organizer
      日中分野別ハイレベル研究者交流会2019:医薬・健康領域
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] イソプレノイド生合成経路は新発見の宝庫2019

    • Author(s)
      葛山 智久
    • Organizer
      日本農芸化学会2019年度大会シンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 複雑骨格を創成する革新的生合成マシナリーの開拓と精密機能解析2018

    • Author(s)
      葛山 智久
    • Organizer
      新学術領域生合成リデザイン第5回公開シンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Understanding biochemical mechanism in natural product biosynthesis2018

    • Author(s)
      葛山 智久
    • Organizer
      Egypt/Japan Joint Seminar on Natural Products
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 放線菌のメロテルペノイド生合成における普遍的脱アミノ化反応に関する研究2018

    • Author(s)
      葛山 智久
    • Organizer
      日本応用酵素協会第44回研究発表会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Unraveling the biosynthesis of the phosphonate antibiotic fosfomycin2018

    • Author(s)
      葛山 智久
    • Organizer
      1st German-Japanese Joint Symposium on the Biosynthesis of Natural Products
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Activation of cryptic gene clusters led to the discovery of novel polyketides in Streptomyces2018

    • Author(s)
      葛山 智久
    • Organizer
      The 3rd A3 Foresight Symposium on Chemical & Synthetic Biology of Natural Products
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ペプチジルヌクレオシド系抗生物質Amipurimycinの生合成に関する研究2018

    • Author(s)
      池内 秀雄、白石 太郎、新家 一男、西山 真、葛山 智久
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 原核生物由来テルペン合成酵素の探索と解析2018

    • Author(s)
      村井 恵一、新家 一男、西山 真、葛山 智久
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 新規thioviridamide類縁体の創出と生合成酵素の機能解析2018

    • Author(s)
      工藤 慧、小岩井 花恵、新家 一男、池田 治生、西山 真、葛山 智久
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Trichoderma属糸状菌由来Brasilane型セスキテルペン合成酵素の機能解析2018

    • Author(s)
      寺本 和矢、野中 健一、塩見 和朗、西山 真、葛山 智久
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 放線菌のメロテルペノイド生合成における普遍的脱アミノ化機構の解析2018

    • Author(s)
      野口 智弘、工藤 慧、西山 真、葛山 智久
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] プレニルインドール化合物carquinostatin Aの生合成研究2018

    • Author(s)
      小林 正弥、新家 一男、西山 真、葛山 智久
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Biosynthetic studies on the antituberculous agent caprazamycin2017

    • Author(s)
      Taro Shiraishi
    • Organizer
      Directing Biosynthesis V
    • Place of Presentation
      コベントリー(英国)
    • Year and Date
      2017-03-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Discovery and biosynthetic characterization of a natural product biosynthesized via AmCP-mediated machinery2017

    • Author(s)
      Kenichi Matsuda
    • Organizer
      Directing Biosynthesis V
    • Place of Presentation
      コベントリー(英国)
    • Year and Date
      2017-03-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Novel Polyketides Discovered via Activation of Cryptic Genes from Streptomyces2017

    • Author(s)
      Thong, Wei-li, Kuzuyama, T.
    • Organizer
      US-Japan Seminar on the Biosynthesis of Natural Products for Young Researchers
    • Place of Presentation
      東京工業大学(東京都 目黒区)
    • Year and Date
      2017-03-04
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Biosynthesis of the Nucleoside Antibiotic A-949642017

    • Author(s)
      Shiraishi, T., Nishiyama, M., Kuzuyama, T.
    • Organizer
      US-Japan Seminar on the Biosynthesis of Natural Products for Young Researchers
    • Place of Presentation
      東京工業大学(東京都 目黒区)
    • Year and Date
      2017-03-04
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Discovery and biosynthetic characterization of a natural product biosynthesized via AmCP-mediated machinery2017

    • Author(s)
      Matsuda, K., Hasebe, F., Shiwa, Y., Kanesaki, Y., Tomita, T., Yoshikawa, H., Shin-ya, K., Kuzuyama, T. Nishiyama, M.
    • Organizer
      US-Japan Seminar on the Biosynthesis of Natural Products for Young Researchers
    • Place of Presentation
      東京工業大学(東京都 目黒区)
    • Year and Date
      2017-03-04
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Biosynthesis of a Histone Deacetylase Inhibitor Trichostatin A2017

    • Author(s)
      Kudo K, Shin-ya, K, Nishiyama, M., Kuzuyama, K.
    • Organizer
      Biosynthesis of a Histone Deacetylase Inhibitor Trichostatin A
    • Place of Presentation
      東京工業大学(東京都 目黒区)
    • Year and Date
      2017-03-04
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Activation of cryptic gene clusters led to the discovery of novel polyketides in Streptomyces2017

    • Author(s)
      Thong, Wei-li, Kuzuyama, T.
    • Organizer
      JSPS-NRCT2017
    • Place of Presentation
      バンコク(タイ)
    • Year and Date
      2017-03-01
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Elucidation of Multistep Reaction Cascade Catalyzed by the Diterpene Cyclase CotB22017

    • Author(s)
      Kazuya Teramoto, Makoto Nishiyama, Tomohisa Kuzuyama
    • Organizer
      International Symposium on Biology of Actinomycetes
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Biosynthetic Origin of the Hydroxamic Acid Moiety of Trichostatin A: Identification of Unprecedented Enzymatic Machinery Involved in Hydroxylamine Transfer2017

    • Author(s)
      Kei Kudo, Taro Ozaki, Kazuo Shin-ya, Makoto Nishiyama, Tomohisa Kuzuyama
    • Organizer
      International Symposium on Biology of Actinomycetes
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Novel Polyketides Discovered via Activation of Cryptic Genes from Streptomyces2017

    • Author(s)
      Wei li Thong, Kazuo Shin-ya, Makoto Nishiyama, Tomohisa Kuzuyama
    • Organizer
      International Symposium on Biology of Actinomycetes
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Structural Studies on the Biosynthesis of Cyclolavandulyl Skeleton Catalyzed by an Unprecedented Terpene Synthase2017

    • Author(s)
      Masaya Kobayashi, Takeo Tomita, Makoto Nishiyama, Tomohisa Kuzuyama
    • Organizer
      International Symposium on Biology of Actinomycetes
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Investigating Biosynthetic of the Antituberculous Agent Caprazamycin2017

    • Author(s)
      Taro Shiraishi, Makoto Nishiyama, Tomohisa Kuzuyama
    • Organizer
      International Symposium on Biology of Actinomycetes
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mechanistic insights into terpene cyclization reactions2017

    • Author(s)
      Tomohisa Kuzuyama
    • Organizer
      U.S./Japan Seminar on Natural Product Biosynthesis
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Understanding Biochemical Mechanism in Natural Product Biosynthesis2017

    • Author(s)
      Tomohisa Kuzuyama
    • Organizer
      1st China-Japan Joint Symposium on Natural Product Biosynthesis
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] プレニルインドール化合物carquinostatin Aの生合成研究2017

    • Author(s)
      小林 正弥, 新家 一男, 西山 真, 葛山 智久
    • Organizer
      日本放線菌学会2017年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] カプラザマイシン生合成に関与する特異なメチル基転移酵素の機能解析2017

    • Author(s)
      白石 太郎、五十嵐 雅之、西山 真、葛山 智久
    • Organizer
      日本放線菌学会2017年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 放線菌のメロテルペノイド生合成における普遍的脱アミノ化機構の解析2017

    • Author(s)
      野口智弘, 工藤慧, 西山真, 葛山智久
    • Organizer
      日本放線菌学会2017年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 原核生物由来テルペン合成酵素の探索と解析2017

    • Author(s)
      村井 恵一、新家 一男、西山 真、葛山 智久
    • Organizer
      第27回イソプレノイド研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 複雑骨格を創成する革新的生合成マシナリーの開拓と精密機能解析2017

    • Author(s)
      葛山智久
    • Organizer
      新学術領域「生合成リデザイン」第一回公開シンポジウム
    • Place of Presentation
      東京大学(東京都 文京区)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Understanding biochemical mechanism in natural product biosynthesis2017

    • Author(s)
      Tomohisa Kuzuyama
    • Organizer
      Directing Biosynthesis V
    • Place of Presentation
      コベントリー(英国)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Isolation of isoindolinomycin, a novel isoindolinone-derived natural product discovered via activation of cryptic gene cluster from Streptomyces2017

    • Author(s)
      Thong, Wei-li, Kuzuyama, T.
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都府 東山区)
    • Related Report
      2016 Annual Research Report
  • [Presentation] ペプチジルヌクレオシド系抗生物質Amipurimycinの生合成に関する研究2017

    • Author(s)
      池内秀雄, 新家一男 西山真, 葛山智久
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都府 東山区)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 放線菌のメロテルペノイド生合成における普遍的脱アミノ化機構の解析2017

    • Author(s)
      野口智弘、工藤慧、西山真、葛山智久
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都府 東山区)
    • Related Report
      2016 Annual Research Report
  • [Presentation] カプラザマイシン生合成における小タンパク質を介したアシル化反応の発見2017

    • Author(s)
      白石太郎、廣昇、五十嵐雅之、西山真、葛山智久
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都府 東山区)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Structural and biochemical studies of cytidylyltransferase involved in the biosynthesis of the phosphonate-antibiotic fosfomycin2016

    • Author(s)
      Cho, Su-Hee, Kuzuyama, T.
    • Organizer
      日本放線菌学会2016年度大会
    • Place of Presentation
      東京大学(東京都 文京区)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] Studies on the Biosynthesis of the Peptidyl Nucleoside Antibiotic Amipurimycin2016

    • Author(s)
      池内秀雄、新家一男、西山真、葛山智久
    • Organizer
      日本放線菌学会2016年度大会
    • Place of Presentation
      東京大学(東京都 文京区)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] アシル化反応に関与する新奇キャリアタンパク質の発見2016

    • Author(s)
      白石太郎、廣昇、五十嵐雅之、西山真、葛山智久
    • Organizer
      日本放線菌学会2016年度大会
    • Place of Presentation
      東京大学(東京都 文京区)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] Biosynthesis of novel polyketides discovered via activation of cryptic genes from Streptomyces2016

    • Author(s)
      Thong, Wei-li, Kuzuyama, T.
    • Organizer
      日本放線菌学会2016年度大会
    • Place of Presentation
      東京大学(東京都 文京区)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] Biosynthesis of the antituberculous agent caprazamycin2016

    • Author(s)
      Taro Shiraishi, Makoto Nishiyama and Tomohisa Kuzuyama
    • Organizer
      2016 SIMB Annual Meeting and Exhibition
    • Place of Presentation
      ニューオーリンズ(米国)
    • Year and Date
      2016-07-24
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 微生物由来テルペノイドの生合成研究2016

    • Author(s)
      葛山智久
    • Organizer
      住木・梅澤記念賞受賞講演会
    • Place of Presentation
      学士会館(東京都 千代田区)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 放線菌の生産するヌクレオシド系抗結核性抗生物質に関する研究2016

    • Author(s)
      葛山智久
    • Organizer
      HiHA 第8回 Workshop「代謝と健康」
    • Place of Presentation
      広島大学(広島県 東広島市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Remarks] こんな反応をする酵素があったのか? ~電子環状反応を触媒する世界初の酵素の発見~

    • URL

      https://www.a.u-tokyo.ac.jp/topics/topics_20210215-1.html

    • Related Report
      2020 Annual Research Report
  • [Remarks] 東大と大阪市大、電子環状反応を触媒する酵素を発見

    • URL

      https://www.nikkei.com/article/DGXLRSP605110_V10C21A2000000/

    • Related Report
      2020 Annual Research Report
  • [Remarks] プリン塩基アナログの2-アミノプリンを含む天然化合物の生合成起源を同定

    • URL

      https://www.a.u-tokyo.ac.jp/topics/topics_20190806-1.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] 生物活性に重要なカルバゾール環の新しい形成機構を解明

    • URL

      https://www.a.u-tokyo.ac.jp/topics/topics_20190823-1.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] 工藤慧君の論文がJ Am Chem Socに掲載されました

    • URL

      http://park.itc.u-tokyo.ac.jp/biotec-res-ctr/saiboukinou/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 富田武郎助教の論文がACS Chem Biolに掲載されました

    • URL

      http://park.itc.u-tokyo.ac.jp/biotec-res-ctr/saiboukinou/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 2つの反応を触媒するテルペン合成酵素の構造と反応機構を解明

    • URL

      http://www.a.u-tokyo.ac.jp/topics/2017/20171020-1.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 天然由来ヒストン脱アセチル化酵素阻害物質の生合成機構の解明

    • URL

      http://www.a.u-tokyo.ac.jp/topics/2017/20170622-1.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 葛山智久准教授が住木・梅澤記念賞を受賞しました。

    • URL

      http://park.itc.u-tokyo.ac.jp/biotec-res-ctr/saiboukinou/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 研究概要

    • URL

      http://park.itc.u-tokyo.ac.jp/biotec-res-ctr/saiboukinou/research/index.html

    • Related Report
      2016 Annual Research Report

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Published: 2016-07-04   Modified: 2022-01-27  

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