Redesign of biosynthetic machineries based on molecular and genome evolution of plant specialized metabolisms

• Project Area: Creation of Complex Functional Molecules by Rational Redesign of Biosynthetic Machineries
• Project/Area Number: 16H06454
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Chiba University
• Principal Investigator: Yamazaki Mami 千葉大学, 大学院薬学研究院, 教授 (70222370)
• Project Period (FY): 2016-06-30 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥72,020,000 (Direct Cost: ¥55,400,000、Indirect Cost: ¥16,620,000); Fiscal Year 2020: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000); Fiscal Year 2019: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000); Fiscal Year 2018: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000); Fiscal Year 2017: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000); Fiscal Year 2016: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
• Keywords: アルカロイド / ゲノム / トランスクリプトーム / メタボローム / 分子進化 / 二次代謝 / 生合成 / 植物二次代謝 / 脱炭酸酵素 / カンプトテシン / ゲノム解析 / メタボローム解析 / 植物アルカロイド / メタボロミクス / 計算化学 / リジン脱炭酸 / 代謝拡張 / 統合オミクス / メタボロム / ゲノム編集 / ゲノム進化 / バイオテクノロジー / 植物 / 酵素 / 生体機能利用

Outline of Final Research Achievements

Plant metabolites are important resource with in chemical diversity and biological activity. In this study, molecular evolution of chemodiversity in plants is main theme and following subjects were studied. (1) Molecular mechanism of bifunctional lysine/ornithine decarboxylase that is the key enzyme in biosynthesis of lysine derived alkaloids. (2)Integral omics aproaches to reveal the whole biossynthetic pathway of camptothecin.

Academic Significance and Societal Importance of the Research Achievements

植物による物質生産は地球上のバイオマスの大半を占めておりこれを有効利用することは、持続可能な文明社会のに維持のために不可欠な技術である。本研究で明らかにされた植物性アルカロイド生産の分子メカニズム並びに分子進化に関する知見は将来の合成生物学、代謝工学による医薬品原料化合物の合理的な物質生産に応用展開が可能である。

Research Products

• [Journal Article] Chromosome-level genome assembly of Ophiorrhiza pumila reveals the evolution of camptothecin biosynthesis (2021)
  • Author(s): Rai Amit、Hirakawa Hideki、Nakabayashi Ryo、Kikuchi Shinji、Hayashi Koki、Rai Megha、Tsugawa Hiroshi、Nakaya Taiki、Mori Tetsuya、Nagasaki Hideki、Fukushi Runa、Kusuya Yoko、Takahashi Hiroki、Uchiyama Hiroshi、Toyoda Atsushi、Hikosaka Shoko、Goto Eiji、Saito Kazuki、Yamazaki Mami
  • Journal Title: Nature Communications Volume: 12 Issue: 1 Pages: 405-405
  • DOI: 10.1038/s41467-020-20508-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] DFT Study on the Biosynthesis of Preasperterpenoid A: Role of Secondary Carbocations in the Carbocation Cascade (2020)
  • Author(s): Sato Hajime、Yamazaki Mami、Uchiyama Masanobu
  • Journal Title: Chemical and Pharmaceutical Bulletin Volume: 68 Issue: 5 Pages: 487-490
  • DOI: 10.1248/cpb.c20-00037
  • NAID: 130007838788
  • ISSN: 0009-2363, 1347-5223
  • Year and Date: 2020-05-01
  • Peer Reviewed / Open Access
• [Journal Article] Multiomics-based characterization of specialized metabolites biosynthesis in Cornus Officinalis (2020)
  • Author(s): Rai Amit、Rai Megha、Kamochi Hidetaka、Mori Tetsuya、Nakabayashi Ryo、Nakamura Michimi、Suzuki Hideyuki、Saito Kazuki、Yamazaki Mami
  • Journal Title: DNA Research Volume: 27 Issue: 2 Pages: 1-15
  • DOI: 10.1093/dnares/dsaa009
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Inherent atomic mobility changes in carbocation intermediates during the sesterterpene cyclization cascade (2019)
  • Author(s): Sato Hajime、Mitsuhashi Takaaki、Yamazaki Mami、Abe Ikuro、Uchiyama Masanobu
  • Journal Title: Beilstein Journal of Organic Chemistry Volume: 15 Pages: 1890-1897
  • DOI: 10.3762/bjoc.15.184
  • Peer Reviewed / Open Access
• [Journal Article] Metabolic diversification of nitrogen‐containing metabolites by the expression of a heterologous lysine decarboxylase gene in Arabidopsis (2019)
  • Author(s): Shimizu Yohei、Rai Amit、Okawa Yuko、Tomatsu Hajime、Sato Masaru、Kera Kota、Suzuki Hideyuki、Saito Kazuki、Yamazaki Mami
  • Journal Title: The Plant Journal Volume: 100 Issue: 3 Pages: 505-521
  • DOI: 10.1111/tpj.14454
  • Peer Reviewed
• [Journal Article] A cheminformatics approach to characterize metabolomes in stable-isotope-labeled organisms (2019)
  • Author(s): Tsugawa Hiroshi、Nakabayashi Ryo、Mori Tetsuya、Yamada Yutaka、Takahashi Mikiko、Rai Amit、Sugiyama Ryosuke、Yamamoto Hiroyuki、Nakaya Taiki、Yamazaki Mami、Kooke Rik、Bac-Molenaar Johanna A.、Oztolan-Erol Nihal、Keurentjes Joost J. B.、Arita Masanori、Saito Kazuki
  • Journal Title: Nature Methods Volume: 16 Issue: 4 Pages: 295-298
  • DOI: 10.1038/s41592-019-0358-2
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The complexity of intercellular localisation of alkaloids revealed by single‐cell metabolomics (2019)
  • Author(s): Yamamoto Kotaro、Takahashi Katsutoshi、Caputi Lorenzo、Mizuno Hajime、Rodriguez‐Lopez Carlos E.、Iwasaki Tetsushi、Ishizaki Kimitsune、Fukaki Hidehiro、Ohnishi Miwa、Yamazaki Mami、Masujima Tsutomu、O'Connor Sarah E.、Mimura Tetsuro
  • Journal Title: New Phytologist Volume: 224 Issue: 2 Pages: 848-859
  • DOI: 10.1111/nph.16138
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Transcriptome analysis of Pueraria candollei var. mirifica for gene discovery in the biosyntheses of isoflavones and miroestrol (2019)
  • Author(s): Suntichaikamolkul Nithiwat、Tantisuwanichkul Kittitya、Prombutara Pinidphon、Kobtrakul Khwanlada、Zumsteg Julie、Wannachart Siriporn、Schaller Hubert、Yamazaki Mami、Saito Kazuki、De-eknamkul Wanchai、Vimolmangkang Sornkanok、Sirikantaramas Supaart
  • Journal Title: BMC Plant Biology Volume: 19 Issue: 1
  • DOI: 10.1186/s12870-019-2205-0
• [Journal Article] Comparative transcriptome analyses of three medicinal Forsythia species and prediction of candidate genes involved in secondary metabolisms (2018)
  • Author(s): Sun, L., Rai, A., Rai, M., Nakamura, M., Kawano, N., Yoshimatsu, K., Suzuki, H., Kawahara, N., Saito, K., Yamazaki, M
  • Journal Title: J. Nat. Med. Volume: 72 Issue: 4 Pages: 867-881
  • DOI: 10.1007/s11418-018-1218-6
  • NAID: 40021661236
  • Peer Reviewed
• [Journal Article] Structural and computational bases for dramatic skeletal rearrangement in anditomin biosynthesis (2018)
  • Author(s): Nakashima, Y., Mitsuhashi, T., Matsuda, Y., Senda, M., Sato, H., Yamazaki, M., Uchiyama, M., Senda, T. & Abe, I.
  • Journal Title: JACS Volume: 140 Issue: 30 Pages: 9743-9750
  • DOI: 10.1021/jacs.8b06084
  • Peer Reviewed
• [Journal Article] De Novo Transcriptome assembly and characterization of Lithospermum officinale to discover putative genes involved in specialized metabolites biosynthesis (2018)
  • Author(s): Rai, A., Nakaya, T., Shimizu, Y., Rai, M., Nakamura, M., Suzuki, H., Saito, K., Yamazaki, M.
  • Journal Title: Planta Med Volume: 84 Issue: 12/13 Pages: 920-934
  • DOI: 10.1055/a-0630-5925
  • Peer Reviewed
• [Journal Article] Molecular Components of Arabidopsis Intact Vacuoles Clarified with Metabolomic and Proteomic Analyses (2018)
  • Author(s): Miwa Ohnishi, Aya Anegawa, Yuko Sugiyama, Kazuo Harada, Akira Oikawa, Yasumune Nakayama, Fumio Matsuda, Yukiko Nakamura, Ryosuke Sasaki, Chizuko Shichijo Patrick G. Hatcher, Hidehiro Fukaki, Shigehiko Kanaya, Koh Aoki, Mami Yamazaki, Eiichiro Fukusaki, Kazuki Saito, Tetsuro Mimura
  • Journal Title: Plant and Cell Physiology Volume: 69 Pages: 1353-1362
  • DOI: 10.1093/pcp/pcy069
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Computational Study on a Puzzle in the Biosynthetic Pathway of Anthocyanin: Why is an Enzymatic Oxidation/ Reduction Process Required for a Simple Tautomerization? (2018)
  • Author(s): Hajime Sato, Chao Wang, Mami Yamazaki, Kazuki Saito, Masanobu Uchiyama
  • Journal Title: PLoS ONE Volume: 13 Issue: 6 Pages: e0198944-e0198944
  • DOI: 10.1371/journal.pone.0198944
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Computational Studies on Biosynthetic Carbocation Rearrangements Leading to Quiannulatene: Initial Conformation Regulates Biosynthetic Route, Stereochemistry, and Skeleton Type (2018)
  • Author(s): Sato Hajime、Mitsuhashi Takaaki、Yamazaki Mami、Abe Ikuro、Uchiyama Masanobu
  • Journal Title: Angewandte Chemie International Edition Volume: 57 Issue: 45 Pages: 14752-14757
  • DOI: 10.1002/anie.201807139
  • Peer Reviewed
• [Journal Article] Focused genome mining of structurally related sesterterpenes: enzymatic formation of enantiomeric and diastereomeric products (2017)
  • Author(s): Narita, K., Sato, H., Minami, A., Kudo, K., Gao, L., Liu, C., Ozaki, T., Kodama, M.., Lei, X., Taniguchi, T., Monde, K., Yamazaki, M., Uchiyama, M., Oikawa, H.
  • Journal Title: Organic letters Volume: 19 Issue: 24 Pages: 6696-6699
  • DOI: 10.1021/acs.orglett.7b03418
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification of Serratane Synthase Gene from the Fern Lycopodium clavatum (2017)
  • Author(s): Saga Yusuke、Araki Takeshi、Araya Hiroshi、Saito Kazuki、Yamazaki Mami、Suzuki Hideyuki、Kushiro Tetsuo
  • Journal Title: Organic Letters Volume: 19 Issue: 3 Pages: 496-499
  • DOI: 10.1021/acs.orglett.6b03659
  • Peer Reviewed
• [Journal Article] Lysine-derived Alkaloids: Overview and Update on Biosynthesis and Medicinal Applications with Emphasis on Quinolizidine Alkaloids (2017)
  • Author(s): Bunsupa Somnuk、Yamazaki Mami、Saito Kazuki
  • Journal Title: Mini-Reviews in Medicinal Chemistry Volume: 17 Issue: 12 Pages: 1002-1012
  • DOI: 10.2174/1389557516666160506151213
  • Peer Reviewed
• [Journal Article] Function of AP2/ERF Transcription Factors Involved in the Regulation of Specialized Metabolism in Ophiorrhiza pumila Revealed by Transcriptomics and Metabolomics (2016)
  • Author(s): Udomsom Nirin、Rai Amit、Suzuki Hideyuki、Okuyama Jun、Imai Ryosuke、Mori Tetsuya、Nakabayashi Ryo、Saito Kazuki、Yamazaki Mami
  • Journal Title: Frontiers in Plant Science Volume: 7 Pages: 1861-1861
  • DOI: 10.3389/fpls.2016.01861
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An MYB transcription factor regulating specialized metabolisms in <i>Ophiorrhiza pumila</i> (2016)
  • Author(s): Rohani Emelda Rosseleena、Chiba Motoaki、Kawaharada Miki、Asano Takashi、Oshima Yoshimi、Mitsuda Nobutaka、Ohme-Takagi Masaru、Fukushima Atsushi、Rai Amit、Saito Kazuki、Yamazaki Mami
  • Journal Title: Plant Biotechnology Volume: 33 Issue: 1 Pages: 1-9
  • DOI: 10.5511/plantbiotechnology.15.1117a
  • NAID: 130005146721
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed / Open Access
• [Journal Article] RNA-seq Transcriptome Analysis of Panax japonicus, and Its Comparison with Other Panax Species to Identify Potential Genes Involved in the Saponins Biosynthesis (2016)
  • Author(s): Rai Amit、Yamazaki Mami、Takahashi Hiroki、Nakamura Michimi、Kojoma Mareshige、Suzuki Hideyuki、Saito Kazuki
  • Journal Title: Frontiers in Plant Science Volume: 7 Pages: 481-481
  • DOI: 10.3389/fpls.2016.00481
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular Evolution and Functional Characterization of a Bifunctional Decarboxylase Involved in Lycopodium Alkaloid Biosynthesis (2016)
  • Author(s): Bunsupa Somnuk、Hanada Kosuke、Maruyama Akira、Aoyagi Kaori、Komatsu Kana、Ueno Hideki、Yamashita Madoka、Sasaki Ryosuke、Oikawa Akira、Saito Kazuki、Yamazaki Mami
  • Journal Title: Plant Physiology Volume: 171 Issue: 4 Pages: 2432-2444
  • DOI: 10.1104/pp.16.00639
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Onocerin Biosynthesis Requires Two Highly Dedicated Triterpene Cyclases in a FernLycopodium clavatum (2016)
  • Author(s): Araki Takeshi、Saga Yusuke、Marugami Momo、Otaka Junnosuke、Araya Hiroshi、Saito Kazuki、Yamazaki Mami、Suzuki Hideyuki、Kushiro Tetsuo
  • Journal Title: ChemBioChem Volume: 17 Issue: 4 Pages: 288-290
  • DOI: 10.1002/cbic.201500663
  • Peer Reviewed / Int'l Joint Research
• [Presentation] カンプトテシン生合成解明を目指した統合オミクス科学 (2021)
  • Author(s): 山崎真巳、ライアミット、北村智、齊藤和季
  • Organizer: 日本薬学会第141年会
• [Presentation] Whole Genome Assembly and Characterization of Medicinal Plant Sophora flavescens (2020)
  • Author(s): Amit Rai, Yohei Shimizu, Hideki Hirakawa, Mami Yamazaki, Kazuki Saito
  • Organizer: Plant & Animal Genome Conference XXVIII, San Diego, USA.
  • Int'l Joint Research
• [Presentation] テルペン生合成での多段階カスケード反応の理論解析と実験的改変 (2019)
  • Author(s): 佐藤玄，山崎真巳，内山真新
  • Organizer: 第22回理論化学討論会
• [Presentation] シロイヌナズナへのリジン脱炭酸酵素遺伝子導入による新規代謝経路誘導 (2019)
  • Author(s): 清水陽平，Rai Amit，斉藤和季，山崎真巳
  • Organizer: 新学術領域研究「生合成リデザイン」第3回若手シンポジウム（千葉）
• [Presentation] シス調節配列のモチーフ解析と共発現解析の統合によるカンプトテシン生合成候補遺伝子の探索 (2019)
  • Author(s): 林剛生，Rai Amit，斉藤和季，山崎真巳
  • Organizer: 新学術領域研究「生合成リデザイン」第3回若手シンポジウム（千葉）
• [Presentation] シロイヌナズナへのリジン脱炭酸酵素遺伝子導入による化学多様性の拡張 (2019)
  • Author(s): 清水陽平，Amit Rai，佐藤大，鈴木秀幸，斉藤和季，山崎真巳
  • Organizer: 第37回日本植物細胞分子生物学会（京都）
• [Presentation] ゲノム編集によるカンプトテシン生合成経路関連ERF転写因子の機能解析 (2019)
  • Author(s): 蓬田梨那，矢野亮介，Amit Rai，刑部祐里子，斉藤和季，山崎真巳
  • Organizer: 第37回日本植物細胞分子生物学会（京都）
• [Presentation] 植物ゲノム情報に基づく抗がん剤カンプトテシン生合成の解明 (2019)
  • Author(s): 山崎真巳
  • Organizer: 第63回日本薬学会関東支部大会 若手シンポジウム（東京）
  • Invited
• [Presentation] 薬用植物における物質生産の分子基盤を探る (2019)
  • Author(s): 山崎真巳
  • Organizer: 日本生物環境工学会2019年千葉大会（千葉）
  • Invited
• [Presentation] シロイヌナズナへのリジン脱炭酸酵素遺伝子導入による化学多様性の拡張 (2019)
  • Author(s): 清水陽平，Amit Rai，鈴木秀幸，斉藤和季，山崎真巳
  • Organizer: 第13回メタボロームシンポジウム（つくば）
• [Presentation] シロイヌナズナへのリジン脱炭酸酵素遺伝子導入による化学多様性の拡張 (2019)
  • Author(s): 清水陽平，Amit Rai，鈴木秀幸，齊藤和季，山崎真巳
  • Organizer: 第56回植物化学シンポジウム（株）常磐植物化学研究所創業70周年記念シンポジウム“植物化学研究の基礎と展開”（東京）
• [Presentation] シス調節配列のモチーフ解析と共発現解析の統合によるカンプトテシン生合成候補遺伝子の探索 (2019)
  • Author(s): 林剛生，Amit Rai，斉藤和季，山崎真巳
  • Organizer: 第56回植物化学シンポジウム (株)常磐植物化学研究所創業70周年記念シンポジウム“植物化学研究の基礎と展開”（東京）
• [Presentation] シス調節配列のモチーフ解析と共発現解析の統合によるカンプトテシン生合成遺伝子の探索 (2019)
  • Author(s): 林剛生, Amit Rai，斉藤和季，山崎真巳
  • Organizer: 文部科学省科学研究費助成事業 新学術領域研究「学術研究支援基盤形成」『先進ゲノム解析研究推進プラットフォーム（先進ゲノム支援）』－拡大班会議－（名古屋）
• [Presentation] Neo-functionalization of enzymes commits to biosynthesis of bioactive alkaloids (2019)
  • Author(s): Mami Yamazaki
  • Organizer: 第60回日本植物生理学会年会（名古屋）シンポジウム「生存戦略としての植物の物質代謝」
  • Invited
• [Presentation] Comparative genomics to understand evolution of alkaloid biosynthesis and diversification (2019)
  • Author(s): Rai, A., Nakabayashi R., Tsugawa, H., Nakaya, T., Mori, T., Takahashi, H., Kikuchi, S., Saito K, Yamazaki M.
  • Organizer: 第60回日本植物生理学会年会（名古屋）
• [Presentation] L/ODCとADC、DapDCの構造機能解析 (2019)
  • Author(s): 佐藤玄、岩崎わかな、栗原陽平、村上実月、白水美香子、内山真伸、齊藤和季、山崎真巳
  • Organizer: 第60回日本植物生理学会年会（名古屋）
• [Presentation] L/ODCの機能改変によるADC、DapDC活性の獲得 (2019)
  • Author(s): 佐藤玄、岩崎わかな、栗原陽平、村上実月、白水美香子、内山真伸、齊藤和季、山崎真巳
  • Organizer: 日本薬学会台139年会（幕張）
• [Presentation] シロイヌナズナへのリジン脱炭酸酵素遺伝子導入による新規代謝経路誘導と化学多様性の拡張 (2019)
  • Author(s): 清水陽平、アミットライ、佐藤大、鈴木秀幸、齊藤和季、山崎真巳
  • Organizer: 日本薬学会台139年会（幕張）
• [Presentation] カンプトテシン生合成解明のためのオミクス解析． (2018)
  • Author(s): 山崎真巳，ライ・アミット，中谷泰貴，蓬田梨那，矢野涼介，齊藤和季
  • Organizer: 第36回日本植物細胞分子生物学会大会（金沢）
• [Presentation] System biology approach to elucidate camptothecin niosynthesis pathway in-planta. (2018)
  • Author(s): Amit Rai, Hideki Hirakawa, Ryo Nakabayashi, Hiroshi Tsugawa, Taiki Nakaya, Tetsuya Mori, Hideyuki Suzuki, Hiroki Takahashi, Kazuki Saito, Mami Yamazaki
  • Organizer: 第36回日本植物細胞分子生物学会大会（金沢）
• [Presentation] Understanding biosynthesis of aconitine-type diterpene alkaloids in Aconitum japonicum. (2018)
  • Author(s): Megha, Amit Rai, Tetsuya Mori, Ryo Nakabayashi, Michimi Nakamura, Mareshige Kojoma, Hideyuki Suzuki, Hiroki Takahashi, Kazuki Saito, Mami Yamazaki
  • Organizer: 第36回日本植物細胞分子生物学会大会（金沢）
• [Presentation] シロイヌナズナへのリジン／オルニチン脱炭酸酵素遺伝子導入によるカダベリン代謝誘導． (2018)
  • Author(s): 清水陽平，大川結子，佐藤大，鈴木秀幸，Amit Rai，齊藤和季，山崎真巳
  • Organizer: 日本生薬学会第65回年会（広島）
• [Presentation] 植物二次代謝のゲノム進化に学ぶ生合成デザイン． (2018)
  • Author(s): 山崎真巳
  • Organizer: 新学術領域研究（研究領域提案型）生物合成系の再設計による複雑骨格機能分子の革新的創成科学（生合成リデザイン）第五回公開シンポジウム（千葉）
  • Invited
• [Presentation] 安定同位体標識を用いたカンプトテシン生合成の動的プロファイリング (2018)
  • Author(s): 中谷泰貴、Amit Rai、中林亮、森哲哉、齊藤和季、山崎真巳
  • Organizer: 日本薬学会第138年会（金沢）
• [Presentation] ゲノム編集によるカンプト恵心生合成経路関連遺伝子の機能解析系の確立 (2018)
  • Author(s): 矢野涼介、Rai Amit、刑部祐里子、齊藤和季、山崎真巳
  • Organizer: 日本薬学会第138年会（金沢）
• [Presentation] Lysine/Ornithine Decarboxylaseの理論解析 (2018)
  • Author(s): 佐藤玄、岩崎わかな、白水美香子、内山真伸、齊藤和季、山崎真巳
  • Organizer: 日本薬学会第138年会（金沢）
• [Presentation] Integrative comics approach to elucidate camptothecin biosynthesis (2018)
  • Author(s): Amit Rai, Ryo Nakabayashi, Taiki Nakaya, Tetsuya Mori, Hideyuki Suzuki, Kazuki Saito, Mami Yamazaki
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] アルカロイド生合成遺伝子を発現したシロイヌナズナにおける新規代謝物生産 (2018)
  • Author(s): 清水陽平、佐藤大、鈴木秀幸、齊藤和季、山崎真巳
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] 植物二次代謝のゲノム進化に学ぶ生合成デザイン (2017)
  • Author(s): 山崎真巳
  • Organizer: 新学術領域研究（研究領域提案型）生物合成系の再設計による複雑骨格機能分子の革新的創成科学（生合成リデザイン）第3回公開シンポジウム
  • Invited
• [Presentation] Molecular evolution of bifunctional lysine/ornithine decarboxylase leading to alkaloid biosynthesis in plants (2017)
  • Author(s): Mami Yamazaki
  • Organizer: 9th US-Japan Seminar on the Biosynthesis of Natural Products, California, USA
  • Int'l Joint Research / Invited
• [Presentation] Molecular evolution of alkaloid biosynthesis in plants (2017)
  • Author(s): Mami Yamazaki
  • Organizer: The 4th Joint Symposium on Pharmaceutical Sciences between Graduate School of Pharmaceutical Sciences, Chiba University and College of Pharmacy, Seoul National University
  • Int'l Joint Research / Invited
• [Presentation] 植物二次代謝のゲノム進化に学ぶ生合成デザイン (2017)
  • Author(s): 山崎真巳
  • Organizer: 新学術領域研究「生合成リデザイン」第一回公開シンポジウム
  • Invited
• [Presentation] 植物アルカロイド生合成の分子進化 (2016)
  • Author(s): 山崎真巳
  • Organizer: 第53回植物化学シンポジウム
  • Invited
• [Presentation] Gene discovery for camptothecin biosynthesis by integral analyses of transcriptome and metabolome (2016)
  • Author(s): Mami Yamazaki
  • Organizer: International PSE Symposium, Plant Omics and Biotechnology for Human Health
  • Int'l Joint Research / Invited
• [Presentation] Integration of transcriptomics and metabolomics: an application to functional genomics in medicinal plants (2016)
  • Author(s): Mami Yamazaki
  • Organizer: The 26th Federation of Asian Pharmaceutical Associations Congress; FAPA 2016
  • Int'l Joint Research / Invited
• [Presentation] 植物におけるリジン由来アルカロイド生合成の分子進化 (2016)
  • Author(s): 山崎真巳
  • Organizer: 第60回日本薬学会関東支部大会 日本薬学会関東支部 若手シンポジウム
  • Invited
• [Presentation] 植物のアルカロイド生合成機構から学ぶこと (2016)
  • Author(s): 山崎真巳
  • Organizer: 第51回天然物化学談話会
  • Invited
• [Presentation] Gene discovery of specialized metabolisms by genomics, transcriptomics and metabolomics (2016)
  • Author(s): Mami Yamazaki
  • Organizer: Plant Biology Europe EPSO/FESPB 2016 Congress
  • Invited
• [Book] 基礎から学ぶ植物代謝生化学 (2018)
  • Author(s): 水谷 正治、士反 伸和、杉山 暁史
  • Total Pages: 328
  • Publisher: 羊土社
  • ISBN: 9784758120906
• [Book] アルカロイドの科学 (2017)
  • Author(s): 高山廣光編、山崎真巳（分担執筆）
  • Total Pages: 560
  • Publisher: 化学同人
  • ISBN: 9784759814187
• [Book] 植物学の百科事典 (2016)
  • Author(s): 日本植物学会、三村 徹郎編（分担執筆）
  • Total Pages: 800
  • Publisher: 丸善出版
  • ISBN: 9784621300381
• [Remarks] 千葉大学大学院薬学研究院遺伝子資源応用研究室Project
  • URL: http://www.p.chiba-u.jp/lab/idenshi/project.html
• [Remarks] 千葉大学大学院薬学研究院遺伝子資源応用研究室Publication
  • URL: http://www.p.chiba-u.jp/lab/idenshi/publication.html