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Roles of phytohormone in plant reproduction

Planned Research

Project AreaDetermining the principles of the birth of new plant species: molecular elucidation of the lock-and-key systems in sexual reproduction
Project/Area Number 16H06468
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNagoya University

Principal Investigator

Ueguchi-Tanaka Miyako  名古屋大学, 生物機能開発利用研究センター, 教授 (70377795)

Co-Investigator(Kenkyū-buntansha) 松岡 信  名古屋大学, 生物機能開発利用研究センター, 教授 (00270992)
永江 峰幸  名古屋大学, シンクロトロン光研究センター, 助教 (90735771)
渡邉 信久  名古屋大学, シンクロトロン光研究センター, 教授 (70212321)
Project Period (FY) 2016-06-30 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥119,600,000 (Direct Cost: ¥92,000,000、Indirect Cost: ¥27,600,000)
Fiscal Year 2020: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2019: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2018: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2017: ¥29,250,000 (Direct Cost: ¥22,500,000、Indirect Cost: ¥6,750,000)
Fiscal Year 2016: ¥31,850,000 (Direct Cost: ¥24,500,000、Indirect Cost: ¥7,350,000)
Keywordsgibberellin / GID1 / 進化 / X線結晶構造解析 / ジベレリン不活化酵素 / ジベレリン合成酵素 / ジベレリンシグナル伝達 / ジベレリン / 受容体 / X線構造解析 / ジベレリン生合成酵素 / 構造解析 / イネの生殖 / 植物の生殖 / 生殖 / 植物の生殖過程
Outline of Final Research Achievements

Gibberellins (GAs) are perceived by its nuclear receptor GID1. Previously, we found that the induction of antheridium in ferns is mediated by the GID1-GA system (Science 2014). Therefore, it is considered that GA was born as a reproductive hormone. In addition, the structural analysis of GID1-GA let me know that the structural information was important for understanding the evolutional function of GA.
In this study, we (1) investigated GA-GID1 binding from ferns to seed plants, and (2) performed structural and functional analyses of GA synthesis and inactivation enzymes. Based on these analyses, we hypothesized that an evolutionary arms race occurs in the mechanism of GA production and inactivation in plant reproduction.

Academic Significance and Societal Importance of the Research Achievements

本研究は、生殖過程における GA と受容体の共進化をX 線結晶構造解析と活性相関から明らかにするという極めて独創的な試みである。また、本研究により進められる植物タンパク質の構造解析に対するブレークスルーテクノロジーは、本新学術内だけではなく、植物研究全体に対して構造解析をより確実に行う技術であり、今後様々な植物生理現象や応用研究に波及できる可能性がある。

Report

(6 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (41 results)

All 2021 2020 2019 2018 2017 2016

All Journal Article (14 results) (of which Int'l Joint Research: 2 results,  Open Access: 6 results,  Peer Reviewed: 5 results,  Acknowledgement Compliant: 1 results) Presentation (25 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (2 results)

  • [Journal Article] Making the “Green Revolution” Truly Green: improving crop nitrogen use efficiency2021

    • Author(s)
      Wang Fanmiao、Yoshida Hideki、Matsuoka Makoto
    • Journal Title

      Plant and Cell Physiology

      Volume: - Issue: 6 Pages: 942-947

    • DOI

      10.1093/pcp/pcab051

    • Related Report
      2020 Annual Research Report
    • Open Access
  • [Journal Article] A common allosteric mechanism regulates homeostatic inactivation of auxin and gibberellin2020

    • Author(s)
      Takehara Sayaka、Sakuraba Shun、Mikami Bunzo、Yoshida Hideki、Yoshimura Hisako、Itoh Aya、Endo Masaki、Watanabe Nobuhisa、Nagae Takayuki、Matsuoka Makoto、Ueguchi-Tanaka Miyako
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1

    • DOI

      10.1038/s41467-020-16068-0

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Future Strategy of Breeding: Learn by Two Important Genes of Miracle Rice2020

    • Author(s)
      Kawai Kyosuke、Ueguchi-Tanaka Miyako、Matsuoka Makoto
    • Journal Title

      Molecular Plant

      Volume: 13 Issue: 6 Pages: 823-824

    • DOI

      10.1016/j.molp.2020.05.001

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Diverse panicle architecture results from various combinations of Prl5/GA20ox4 and Pbl6/APO1 alleles2020

    • Author(s)
      Agata Ayumi、Ando Koki、Ota Sadayuki、Kojima Mikiko、Takebayashi Yumiko、Takehara Sayaka、Doi Kazuyuki、Ueguchi-Tanaka Miyako、Suzuki Takamasa、Sakakibara Hitoshi、Matsuoka Makoto、Ashikari Motoyuki、Inukai Yoshiaki、Kitano Hidemi、Hobo Tokunori
    • Journal Title

      Communications Biology

      Volume: 3 Issue: 1

    • DOI

      10.1038/s42003-020-1036-8

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Evolution of GA Metabolic Enzymes in Land Plants2020

    • Author(s)
      Yoshida Hideki、Takehara Sayaka、Mori Masaki、Ordonio Reynante Lacsamana、Matsuoka Makoto
    • Journal Title

      Plant and Cell Physiology

      Volume: 61 Issue: 11 Pages: 1919-1934

    • DOI

      10.1093/pcp/pcaa126

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Special Issue on Gibberellin: A Fascinating Substance That Still Attracts Plant Scientists2020

    • Author(s)
      Yoshida Hideki、Nakajima Masatoshi、Matsuoka Makoto
    • Journal Title

      Plant and Cell Physiology

      Volume: 61 Issue: 11 Pages: 1829-1831

    • DOI

      10.1093/pcp/pcaa127

    • Related Report
      2020 Annual Research Report
  • [Journal Article] GWAS with principal component analysis identifies a gene comprehensively controlling rice architecture2019

    • Author(s)
      Yano Kenji、Morinaka Yoichi、Wang Fanmiao、Huang Peng、Takehara Sayaka、Hirai Takaaki、Ito Aya、Koketsu Eriko、Kawamura Mayuko、Kotake Kunihiko、Yoshida Shinya、Endo Masaki、Tamiya Gen、Kitano Hidemi、Ueguchi-Tanaka Miyako、Hirano Ko、Matsuoka Makoto
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 116 Issue: 42 Pages: 21262-21267

    • DOI

      10.1073/pnas.1904964116

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Is one‐line hybrid rice coming?2019

    • Author(s)
      Hu Xingming、Wang Fanmiao、Matsuoka Makoto
    • Journal Title

      Journal of Integrative Plant Biology

      Volume: 61 Issue: 8 Pages: 908-910

    • DOI

      10.1111/jipb.12854

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Evolution and diversification of the plant gibberellin receptor GID12018

    • Author(s)
      Yoshida Hideki、Tanimoto Eiichi、Hirai Takaaki、Miyanoiri Yohei、Mitani Rie、Kawamura Mayuko、Takeda Mitsuhiro、Takehara Sayaka、Hirano Ko、Kainosho Masatsune、Akagi Takashi、Matsuoka Makoto、Ueguchi-Tanaka Miyako
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 115 Issue: 33

    • DOI

      10.1073/pnas.1806040115

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] OsIDD2, a zinc finger and INDETERMINATE DOMAIN protein, regulates secondary cell wall formation.2018

    • Author(s)
      Huang, P., Yoshida, H., Yano, K., Kinoshita, S., Kawai, K., Koketsu, E., Hattori, M., Takehara, S., Huang, J., Hirano, K., Ordonio, RL.**, Matsuoka, M., Ueguchi-Tanaka, M.
    • Journal Title

      J Integr Plant Biol.

      Volume: 60 Issue: 2 Pages: 130-143

    • DOI

      10.1111/jipb.12557

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Small Organ Size 1 and Small Organ Size 2/Dwarf and Low Tillering form a Complex to Integrate Auxin and Brassinosteroid Signaling in Rice.2017

    • Author(s)
      Hirano, K., Yoshida, H., Aya, K., Kawamura, M., Hayashi, M., Hobo, T., Sato-Izawa, K., Kitano, H., Ueguchi-Tanaka, M., Matsuoka, M.
    • Journal Title

      Molecular plant

      Volume: 10 Issue: 2 Pages: 590-604

    • DOI

      10.1104/pp.17.00301

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Small Organ Size 1 and Small Organ Size 2/Dwarf and Low Tillering form a Complex to Integrate Auxin and Brassinosteroid Signaling in Rice.2017

    • Author(s)
      Hirano, K.
    • Journal Title

      Mol Plant.

      Volume: n/a Issue: 4 Pages: 590-604

    • DOI

      10.1016/j.molp.2016.12.013

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ジベレリンシグナル伝達 ─ 受容体GID1と抑制因子DELLAの働き2016

    • Author(s)
      吉田英樹、上口(田中)美弥子
    • Journal Title

      生物の科学 遺伝

      Volume: 70 Pages: 361-365

    • Related Report
      2016 Annual Research Report
  • [Journal Article] ある種のシダにおいてジベレリンは時空間的なコミュニケーションツールとして使われてきた2016

    • Author(s)
      田中純夢、上口(田中)美弥子
    • Journal Title

      化学と生物

      Volume: 55 Pages: 2-4

    • Related Report
      2016 Annual Research Report
  • [Presentation] イネの葯に発現するGA3-酸化酵素1の機能と進化的解析2021

    • Author(s)
      河合恭甫, 樫尾徹, 森井南美, 竹原清日, 杉原諒彦, 吉村久子, 伊藤亜矢, 服部将子, 戸田陽介, 小嶋美紀子, 竹林裕美子, 古海弘康, 野々村賢一, 赤木剛士, 榊原均, 北野英己, 松岡信, 上口(田中)美弥子
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] イネ花粉のデンプン合成におけるジベレリンの作用2021

    • Author(s)
      森井南美、杉原諒彦、河合恭甫、樫尾徹、伊藤亜矢、吉村久子、服部将子、竹原清日、松岡信、上口(田中)美弥子
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] イネ花器官におけるジベレリン3酸化酵素1及び2の役割分担2021

    • Author(s)
      鈴木裕人,竹原清日,河合恭輔,杉原諒彦,森井南美,服部将子,吉村久子,上口(田中)美弥子
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] イネ花粉成熟期における糖化機構の解明2021

    • Author(s)
      葉山旺, 河合恭甫, 杉原諒彦, 森井南美, 竹原清日, 水多陽子, 上口(田中)美弥子
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 植物ホルモン関連因子の構造生物学的解析に向けた試み2020

    • Author(s)
      2.杉原諒彦、竹原清日、三上文三、松岡信、上口(田中)美弥子
    • Organizer
      2020年3月日本農芸化学会2020年度大会、福岡
    • Related Report
      2019 Annual Research Report
  • [Presentation] Evolution of unique reproductive system using a high bioactive gibberellin in rice2020

    • Author(s)
      Kawai Kyosuke, Takehara Sayaka, Kashio Toru, Morii Minami, Ito Aya, Furuumi Hiroyasu, Nonomura Ken-ichi, Akagi Takashi, Sakakibara hitoshi, Matsuoka Makoto, Ueguchi-Tanaka Miyako
    • Organizer
      2020年3月、第61回植物生理学会年会、大阪
    • Related Report
      2019 Annual Research Report
  • [Presentation] Bioavtive GAs synthesized by OsGA3ox1 regulate the starch accumulation of rice pollen2020

    • Author(s)
      森井南美、杉原諒彦、河合恭甫、樫尾徹、伊藤亜矢、竹原清日、松岡信、上口(田中)美弥子
    • Organizer
      2020年3月、第61回植物生理学会年会、大阪
    • Related Report
      2019 Annual Research Report
  • [Presentation] イネのGA合成酵素OsGA3ox1の生殖における機能解析2019

    • Author(s)
      河合恭甫、竹原清日、 樫尾徹、 服部将子、 吉村久子、伊藤亜矢、 古海弘康、 野々村賢一、榊原均、松岡信、 上口(田中)美弥子
    • Organizer
      第10回 植物電子顕微鏡若手ワークショップ2019年11月15日
    • Related Report
      2019 Annual Research Report
  • [Presentation] イネ属で特異的に選択・進化してきた「壊れないジベレリン(GA7)を使った雄性生殖戦略2019

    • Author(s)
      7.上口美弥子
    • Organizer
      日本遺伝学会第91回大会ワークショップ、2019年9月11日、福井大学 (招待講演)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 花粉の発達におけるイネジベレリン3酸化酵素1の役割2019

    • Author(s)
      森井南美、杉原諒彦、河合恭甫、樫尾徹、伊藤亜矢、竹原清日、松岡信、上口(田中)美弥子
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Functional analysis of rice Gibberellin 3-oxidase 1 in reproductive organ2019

    • Author(s)
      河合恭甫、竹原清日、樫尾徹、伊藤亜矢、古海弘康、野々村賢一、松岡信、上口(田中)美弥子
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 構造解析及びMDシミュレーションにより明らかとなった植物ホルモン代謝システム2019

    • Author(s)
      竹原 清日、三上 文三、桜庭 俊、松岡 信、上口(田中) 美弥子
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 植物ホルモン・ジベレリンの受容体GID1の分子進化2018

    • Author(s)
      吉田英樹、谷本英一、平井貴章、宮ノ入洋平、三谷理恵、川村真結子、武田光広、竹原清日、平野恒、甲斐庄正恒、赤木剛士、松岡信、上口(田中)美弥子
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] イネのDELLAタンパク質の構造解析への試み2018

    • Author(s)
      杉原諒彦、竹原清日、松岡信、上口(田中)美弥子
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] イネジベレリン2酸化酵素はGAを介した多量体構造形成によりジベレリン代謝を促進する2018

    • Author(s)
      竹原清日、三上文三、河合恭甫、松岡信、上口(田中)美弥子
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 生殖過程において重要な役割をするイネGA3酸化酵素1の解析2018

    • Author(s)
      河合恭甫、竹原清日、樫尾徹、伊藤亜矢、古海弘康、野々村賢一、松岡信、上口(田中)美弥子
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Evolutionary history of a gibberellin receptor, GID1.2017

    • Author(s)
      Ueguchi-Tanaka, M.
    • Organizer
      19th IBC2017.
    • Related Report
      2017 Annual Research Report
  • [Presentation] Evolutionary aspect of a gibberellin receptor, GID1.2017

    • Author(s)
      Ueguchi-Tanaka, M.
    • Organizer
      韓国 東亜大学校 生命資源科学大学との研究交流会
    • Related Report
      2017 Annual Research Report
  • [Presentation] GA受容体GID1の分子進化に関する研究2017

    • Author(s)
      吉田英樹、谷本英一、平野恒、竹原清日、村上紗耶果、川村真結子、松岡信、上口(田中)美弥子
    • Organizer
      第58回日本植物生理学会年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] ジベレリン生合成および代謝酵素の構造解析と機能解析2017

    • Author(s)
      竹原清日、三上文三、河合恭甫、松岡信、上口(田中)美弥子
    • Organizer
      日本農芸化学会2017年度大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] イネの葯特異的に発現をするジベレリン3酸化酵素1の機能解析2017

    • Author(s)
      河合恭甫、竹原清日、松岡信、上口(田中)美弥子
    • Organizer
      日本農芸化学会2017年度大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Antheridiogen determines sex in ferns via a spatiotemporally split gibberellin synthesis pathway.2016

    • Author(s)
      Tanaka, J., Matsuoka, M., Ueguchi-Tanaka, M.
    • Organizer
      22nd IPGSA
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Structural and functional analysis of gibberellin 2-oxidase in rice2016

    • Author(s)
      Takehara, S., Kawai, K., Mikami, B., Matsuoka, M., Ueguchi-Tanaka, M.
    • Organizer
      22nd IPGSA
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Gibberellin 3-oxidase 1 is essential for pollen maturation in Rice.2016

    • Author(s)
      Kawai, K., Takehara, S., Matsuoka, M., Ueguchi-Tanaka, M.
    • Organizer
      22nd IPGSA
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] DELLA, IDD and SCL3 cooperate in the gibberellin feedback system.2016

    • Author(s)
      Yoshida, H., Hirano, K., Matsuoka, M., Ueguchi-Tanaka, M.
    • Organizer
      22nd IPGSA
    • Related Report
      2016 Annual Research Report
  • [Book] Plant Structural Biology: Hormonal Regulations2018

    • Author(s)
      Takehara S., Ueguchi-Tanaka M.
    • Total Pages
      13
    • Publisher
      Springer
    • Related Report
      2018 Annual Research Report
  • [Book] 植物ホルモンの科学第3版2016

    • Author(s)
      上口(田中)美弥子、中嶋正敏
    • Publisher
      講談社
    • Related Report
      2016 Annual Research Report

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Published: 2016-07-04   Modified: 2022-01-27  

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