• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Species barriers in endosperm: key molecular mechanisms of epigenetic regulation

Planned Research

Project AreaDetermining the principles of the birth of new plant species: molecular elucidation of the lock-and-key systems in sexual reproduction
Project/Area Number 16H06471
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionYokohama City University

Principal Investigator

Kinoshita Tetsu  横浜市立大学, 木原生物学研究所, 教授 (60342630)

Co-Investigator(Kenkyū-buntansha) 吉田 健太郎  神戸大学, 農学研究科, 准教授 (40570750)
赤木 剛士  京都大学, 農学研究科, 助教 (50611919)
Project Period (FY) 2016-06-30 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥115,960,000 (Direct Cost: ¥89,200,000、Indirect Cost: ¥26,760,000)
Fiscal Year 2020: ¥21,450,000 (Direct Cost: ¥16,500,000、Indirect Cost: ¥4,950,000)
Fiscal Year 2019: ¥21,840,000 (Direct Cost: ¥16,800,000、Indirect Cost: ¥5,040,000)
Fiscal Year 2018: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
Fiscal Year 2017: ¥22,750,000 (Direct Cost: ¥17,500,000、Indirect Cost: ¥5,250,000)
Fiscal Year 2016: ¥27,560,000 (Direct Cost: ¥21,200,000、Indirect Cost: ¥6,360,000)
Keywords胚乳 / 生殖的隔離 / エピジェネティクス / イネ / エピゲノム / DNA脱メチル化 / 生殖隔離 / ゲノムインプリンティング / ゲノムバランス / 新種誕生 / 生殖 / シロイヌナズナ / DNAメチル化 / 低分子RNA
Outline of Final Research Achievements

The mechanism of species hybridization barrier is arranged in the plant endosperm to avoid heterologous fertilization with different species, which is controlled by not only nucleotide sequence but also epigenetic mechanisms. In this study, in order to elucidate the principle of the birth of new plant species, we clarified the role of FACT histone chaperones, which are essential for DNA demethylation to epigenetically induce reproductive barriers in the endosperm. We also identified basics that species barrier can be overcome by increased ploidy levels in one parent.

Academic Significance and Societal Importance of the Research Achievements

胚乳胚乳の発生は塩基配列レベルのみならず、ゲノムインプリンティングを介したエピジェネティックな機構によりオス由来とメス由来のゲノムのバランスが保たれている。この機構は同種の交雑ではバランスが保たれているが、異種の交雑ではそのバランスが崩れる致死となる。本研究では、その分子機構を明らかにし、異種の交雑においても片親の倍数性を操作するとバランスが復帰して発芽可能な雑種種子が得られることを明らかにした。

Report

(6 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (23 results)

All 2021 2019 2018 2016 Other

All Int'l Joint Research (8 results) Journal Article (11 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 10 results,  Open Access: 8 results,  Acknowledgement Compliant: 1 results) Presentation (1 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (3 results)

  • [Int'l Joint Research] University of California, Davis(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] UC Berkeley(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Seoul National University(韓国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] John Innes Center(英国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Swedish University(スウェーデン)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Yokohama City University/Okayama University(日本)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University Geneva(Switzerland)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Gregor Mendel Institute(Austria)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Persistent directional growth capability in Arabidopsis thaliana pollen tubes after nuclear elimination from the apex2021

    • Author(s)
      Motomura Kazuki、Takeuchi Hidenori、Notaguchi Michitaka、Tsuchi Haruna、Takeda Atsushi、Kinoshita Tetsu、Higashiyama Tetsuya、Maruyama Daisuke
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 2331-2331

    • DOI

      10.1038/s41467-021-22661-8

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mutation of the imprinted gene OsEMF2a induces autonomous endosperm development and delayed cellularization in rice2021

    • Author(s)
      Tonosaki Kaoru、Ono Akemi、Kunisada Megumi、Nishino Megumi、Nagata Hiroki、Sakamoto Shingo、Kijima Saku T、Furuumi Hiroyasu、Nonomura Ken-Ichi、Sato Yutaka、Ohme-Takagi Masaru、Endo Masaki、Comai Luca、Hatakeyama Katsunori、Kawakatsu Taiji、Kinoshita Tetsu
    • Journal Title

      The Plant Cell

      Volume: 33 Pages: 85-103

    • DOI

      10.1093/plcell/koaa006

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Epigenetics and plant reproduction: Multiple steps for responsibly handling succession2021

    • Author(s)
      Ono Akemi、Kinoshita Tetsu
    • Journal Title

      Current Opinion in Plant Biology

      Volume: 61 Pages: 102032-102032

    • DOI

      10.1016/j.pbi.2021.102032

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] DNA demethylation by ROS1a in rice vegetative cells promotes methylation in sperm2019

    • Author(s)
      Kim M. Yvonne、Ono Akemi、Scholten Stefan、Kinoshita Tetsu、Zilberman Daniel、Okamoto Takashi、Fischer Robert L.
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 印刷中 Issue: 19 Pages: 9652-9657

    • DOI

      10.1073/pnas.1821435116

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Endosperm Development2018

    • Author(s)
      Kaoru Tonosaki and Tetsu Kinoshita
    • Journal Title

      eLS

      Volume: - Pages: 1-7

    • DOI

      10.1002/9780470015902.a0020098.pub2

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] FACT complex is required for DNA demethylation at heterochromatin during reproduction inArabidopsis2018

    • Author(s)
      Frost Jennifer M.、Kim M. Yvonne、Park Guen Tae、Hsieh Ping-Hung、Nakamura Miyuki、Lin Samuel J. H.、Yoo Hyunjin、Choi Jaemyung、Ikeda Yoko、Kinoshita Tetsu、Choi Yeonhee、Zilberman Daniel、Fischer Robert L.
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 1 Issue: 20 Pages: 1-10

    • DOI

      10.1073/pnas.1713333115

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A parental tug-of-war2018

    • Author(s)
      Kinoshita Tetsu
    • Journal Title

      Nature Plants

      Volume: 4 Issue: 6 Pages: 329-330

    • DOI

      10.1038/s41477-018-0179-9

    • Related Report
      2018 Annual Research Report
  • [Journal Article] A pharmacological study of Arabidopsis cell fusion between the persistent synergid and endosperm.2018

    • Author(s)
      Motomura K, Kawashima T, Berger F, Kinoshita T, Higashiyama T, Maruyama D.
    • Journal Title

      J Cell Sci.

      Volume: 131 Issue: 2 Pages: 1-6

    • DOI

      10.1242/jcs.204123

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Overcoming the species barrier by ploidy manipulation in the genus Oryza2018

    • Author(s)
      Tonosaki Kaoru、Sekine Daisuke、Ohnishi Takayuki、Ono Akemi、Furuumi Hiroyasu、Kurata Nori、Kinoshita Tetsu
    • Journal Title

      The Plant Journal

      Volume: 93 Issue: 3 Pages: 534-544

    • DOI

      10.1111/tpj.13803

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fertilization-independent Cell-fusion between the Synergid and Central Cell in the Polycomb Mutant2016

    • Author(s)
      Kazuki Motomura, Frederic Berger, Tomokazu Kawashima, Tetsu Kinoshita, Tetsuya Higashiyama, Daisuke Maruyama
    • Journal Title

      Cell Structure and Function

      Volume: 41 Issue: 2 Pages: 121-125

    • DOI

      10.1247/csf.16010

    • NAID

      130005405450

    • ISSN
      0386-7196, 1347-3700
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Dormancy-specific imprinting underlies maternal inheritance of seed dormancy in Arabidopsis thaliana.2016

    • Author(s)
      Piskurewicz U, Iwasaki M, Susaki D, Megies C, Kinoshita T, Lopez-Molina L.
    • Journal Title

      eLIFE

      Volume: 5

    • DOI

      10.7554/elife.19573

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Overcoming the species hybridization barrier by ploidy manipulation in the genus Oryza2021

    • Author(s)
      Tetsu Kinoshita
    • Organizer
      Genome Concept Centennial Symposium
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Remarks] お米(イネ胚乳)の生長を制御する遺伝子を同定; 受粉無しでデンプンを蓄積

    • URL

      https://www.yokohama-cu.ac.jp/news/2020/20201125kinoshita_PC.html

    • Related Report
      2020 Annual Research Report
  • [Remarks] 植物遺伝子発現スイッチの初発イベントを解明

    • URL

      https://www.yokohama-cu.ac.jp/news/2018/20180501kinoshita.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] 木原生物学研究所 木下 哲教授の解説記事がNature Plants誌に掲載されました

    • URL

      https://www.yokohama-cu.ac.jp/news/2018/20180528kinoshita.html

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2016-07-04   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi