| Budget Amount *help |
¥148,850,000 (Direct Cost: ¥114,500,000、Indirect Cost: ¥34,350,000)
Fiscal Year 2020: ¥29,250,000 (Direct Cost: ¥22,500,000、Indirect Cost: ¥6,750,000)
Fiscal Year 2019: ¥29,250,000 (Direct Cost: ¥22,500,000、Indirect Cost: ¥6,750,000)
Fiscal Year 2018: ¥29,250,000 (Direct Cost: ¥22,500,000、Indirect Cost: ¥6,750,000)
Fiscal Year 2017: ¥29,250,000 (Direct Cost: ¥22,500,000、Indirect Cost: ¥6,750,000)
Fiscal Year 2016: ¥31,850,000 (Direct Cost: ¥24,500,000、Indirect Cost: ¥7,350,000)
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| Outline of Final Research Achievements |
We analyzed the impact of the mechanical properties of the developing brain tissue and cells in the control of the fate, position and shape of neurons in the well-organized cortex. We found that neurons switch the actomyosin force generation mechanisms in response to the mechanical stress of the surrounding microenvironment. We also found that the nuclei of newborn neurons are extremely soft due to its special nuclear lamina structure. The soft nucleus squeeze into the narrow tissue spaces against the shear stress by sharp deformation and rotation driven by the dynamic force of microtubule motors. We also proved that dendritic filopodia act as mechanosensors for space exploration and control the spatial distribution of dendritic arbors where collisions between projections do not occur. Furthermore, we developed a gel substrate that reproduces the softness of brain tissue and established a protocol to promote neuronal differentiation from neural stem cells.
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