| Budget Amount *help |
¥84,760,000 (Direct Cost: ¥65,200,000、Indirect Cost: ¥19,560,000)
Fiscal Year 2020: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2019: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2018: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2017: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2016: ¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000)
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| Outline of Final Research Achievements |
We have developed probes adapted for live imaging in various intracellular and extracellular environments, such as acid-resistant green fluorescent protein to achieve fluorescence imaging under acidic conditions, such as lysosomes; bioluminescent low-affinity calcium sensor to observe calcium dynamics in organelles with high calcium concentration, such as endoplasmic reticulum; fluorescent sensor to detect an interaction of intercellular adhesion protein cadherin. We also developed a green photosensitizing fluorescent protein that can produce reactive oxygen species upon light irradiation and is available for spatiotemporally control the disruption of protein and cellular functions.
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