Individuality caused by postnatal neurogenesis

• Project Area: Integrative research toward elucidation of generative brain systems for individuality
• Project/Area Number: 16H06529
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Kyoto University
• Principal Investigator: Imayoshi Itaru 京都大学, 生命科学研究科, 教授 (60543296)
• Project Period (FY): 2016-06-30 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥86,710,000 (Direct Cost: ¥66,700,000、Indirect Cost: ¥20,010,000); Fiscal Year 2020: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000); Fiscal Year 2019: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000); Fiscal Year 2018: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000); Fiscal Year 2017: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000); Fiscal Year 2016: ¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000)
• Keywords: 個性創発 / ニューロン新生 / 神経幹細胞 / 光遺伝学 / 個性 / 生後脳ニューロン新生 / 神経神経 / 神経発生 / 神経再生 / 神経発達 / 海馬 / 嗅球 / 可塑性 / 神経可塑性

Outline of Final Research Achievements

In rodent animal models, we developed behavior monitoring systems to analyze individuality. We introduced data mining technology and applied it to mice behaviors in spatial leaning and memory tasks. We also showed that adult neurogenesis in the olfactory bulb is essential for plasticity in the olfactory-related brain circuit. In addition, we optimized light-controllable gene expression systems and showed this system can control postnatal/adult neurogenesis in the hippocampus.

Academic Significance and Societal Importance of the Research Achievements

動物行動の多様性は、人間社会においては個性として健在しており、その背景にある神経基盤の理解や、創発機構については着目が集まっている。本研究課題においては、マウスをモデル動物を用いて、生後脳ニューロン新生と個性創発機構の関係について、その一旦を明らかにすることができた。また、生後脳ニューロン新生に摂動を与える技術の開発にも成功し、今後の、個性創発機構の研究の重要な手法になると期待されるとともに、脳神経系の再生医学研究への発展についても期待される。

Research Products

• [Int'l Joint Research] Psychology Department/University of Toronto(カナダ)
• [Int'l Joint Research] Department of Developmental Biology/Hagedorn Research Institute(デンマーク)
• [Journal Article] Optimization of light-inducible Gal4/UAS gene expression system in mammalian cells (2020)
  • Author(s): Yamada M., Nagasaki C. S., Suzuki Y., Imayoshi I.
  • Journal Title: iScience Volume: 23 Issue: 9 Pages: 101506-101506
  • DOI: 10.1016/j.isci.2020.101506
  • NAID: 120006884197
  • Peer Reviewed / Open Access
• [Journal Article] Light-induced silencing of neural activity in Rosa26 knock-in and BAC transgenic mice conditionally expressing the microbial halorhodopsin eNpHR3. (2020)
  • Author(s): Imayoshi I, Tabuchi S, Matsumoto M, Kitano S, Miyachi H, Kageyama R, Yamanaka A
  • Journal Title: Sci Rep Volume: 10(1) Issue: 1 Pages: 3191-3191
  • DOI: 10.1038/s41598-020-59984-3
  • NAID: 120006878490
  • Peer Reviewed / Open Access
• [Journal Article] Jag1 Modulates an Oscillatory Dll1-Notch-Hes1 Signaling Module to Coordinate Growth and Fate of Pancreatic Progenitors (2020)
  • Author(s): Seymour Philip Allan、Collin Caitlin Alexis、Egeskov-Madsen Anuska la Rosa、Jorgensen Mette Christine、Shimojo Hiromi、Imayoshi Itaru、de Lichtenberg Kristian Honnens、Kopan Raphael、Kageyama Ryoichiro、Serup Palle
  • Journal Title: Developmental Cell Volume: 52 Issue: 6 Pages: 731-747
  • DOI: 10.1016/j.devcel.2020.01.015
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cholecystokinin-expressing Interneurons of the Medial Prefrontal Cortex Mediate Working Memory Retrieval. (2020)
  • Author(s): Nguyen, R., Venkatesan, S., Binko, M., Yoon Bang, J., Cajanding, J.D., Briggs, C., Sargin, D., Imayoshi, I., Lambe, E.K. and Chul Kim, J.
  • Journal Title: J Neurosci. Volume: 40 Issue: 11 Pages: 2314-2331
  • DOI: 10.1523/jneurosci.1919-19.2020
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Light-mediated Control of Gene Expression in Mammalian Cells. (2020)
  • Author(s): Yamada M, Nagasaki C. S, Ozawa T, Imayoshi I.
  • Journal Title: Neuroscience Research Volume: 152 Pages: 66-77
  • DOI: 10.1016/j.neures.2019.12.018
  • NAID: 120006888020
  • Peer Reviewed / Open Access
• [Journal Article] High Hes1 expression and resultant Ascl1 suppression regulate quiescent vs. active neural stem cells in the adult mouse brain (2019)
  • Author(s): Sueda Risa、Imayoshi Itaru、Harima Yukiko、Kageyama Ryoichiro
  • Journal Title: Genes & Development Volume: 33 Issue: 9-10 Pages: 511-523
  • DOI: 10.1101/gad.323196.118
  • NAID: 120006591371
  • Peer Reviewed / Open Access
• [Journal Article] Enhanced lysosomal degradation maintains the quiescent state of neural stem cells (2019)
  • Author(s): T. Kobayashi, W. Piao, T. Takamura, H. Kori, H. Miyachi, S. Kitano, Y. Iwamoto, M. Yamada, I. Imayoshi, S. Shioda, A. Ballabio, R. Kageyama
  • Journal Title: Nature Communications Volume: 10 Issue: 1 Pages: 5446-5446
  • DOI: 10.1038/s41467-019-13203-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Light Control of the Tet Gene Expression System in Mammalian Cells (2018)
  • Author(s): Yamada Mayumi、Suzuki Yusuke、Nagasaki Shinji C.、Okuno Hiroyuki、Imayoshi Itaru
  • Journal Title: Cell Reports Volume: 25 Issue: 2 Pages: 487-500
  • DOI: 10.1016/j.celrep.2018.09.026
  • NAID: 120006531246
  • Peer Reviewed / Open Access
• [Journal Article] Adult-born neurons facilitate olfactory bulb pattern separation during task engagement. (2018)
  • Author(s): Li W, Chu MW, Wu A, Suzuki Y, Imayoshi I and Komiyama T
  • Journal Title: eLife Volume: 7
  • DOI: 10.7554/elife.33006
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 発生発達期暴露による嗅球神経新生影響～毒性評価としての嗅球利用を探る～ (2020)
  • Author(s): 今吉 格
  • Organizer: 第４７回日本毒性学会学術年会
  • Int'l Joint Research / Invited
• [Presentation] Toward understanding and manipulation of neural bases underlying animal behaviors and psychiatric diseases (2020)
  • Author(s): 今吉 格
  • Organizer: 第43回日本分子生物学会年会シンポジウム
  • Int'l Joint Research / Invited
• [Presentation] Regulatory mechanism of neural stem cells revealed by optical manipulation of gene expressions (2020)
  • Author(s): 今吉 格
  • Organizer: 第11回新潟大学脳研究所共同研究拠点国際シンポジウム
  • Int'l Joint Research / Invited
• [Presentation] 神経幹細胞の制御機構 と生後脳ニューロン新生 (2019)
  • Author(s): 今吉 格
  • Organizer: 第２１回 京都大学・生命科学研究科シンポジウム
  • Invited
• [Presentation] 遺伝子発現の光操作技術 と神経幹細胞研究への応用 (2019)
  • Author(s): 今吉 格
  • Organizer: Chemistry For Neuroscience 2019
  • Invited
• [Presentation] Regulatory mechanism of neural stem cells revealed by optical manipulation of gene expressions (2019)
  • Author(s): 今吉 格
  • Organizer: ExCELLS若手リトリート
  • Invited
• [Presentation] Regulatory mechanism of neural stem cells revealed by optical manipulation of gene expressions (2018)
  • Author(s): 今吉 格
  • Organizer: 第17回幹細胞シンポジウム
  • Invited
• [Presentation] Regulatory mechanism of neural stem cells revealed by optical manipulation of gene expressions (2018)
  • Author(s): 今吉 格
  • Organizer: 第41回日本分子生物学会年会
  • Invited
• [Presentation] 遺伝子発現動態の光操作から明らかになる神経幹細胞の制御機構 (2017)
  • Author(s): 今吉 格
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: アクトシティ浜松
  • Year and Date: 2017-03-29
  • Invited
• [Presentation] 海馬生後ニューロン新生の破綻と発達障害との関係について (2016)
  • Author(s): 今吉 格
  • Organizer: 発達障害研究所公開セミナー 2016
  • Place of Presentation: 愛知県心身障害者コロニー 総合管理事務所1階 講堂
  • Year and Date: 2016-12-22
  • Invited
• [Patent(Industrial Property Rights)] Cry2-CIB1システムを用いたTetシステムの光制御手法 (2018)
  • Inventor(s): 今吉格、山田真弓、鈴木裕輔
  • Industrial Property Rights Holder: 今吉格、山田真弓、鈴木裕輔
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2018-163617
  • Filing Date: 2018