Genetic analysis of regulatory network of proton motive force

• Project Area: New Photosynthesis : Reoptimization of the solar energy conversion system
• Project/Area Number: 16H06555
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: Shikanai Toshiharu 京都大学, 理学研究科, 教授 (70273852)
• Project Period (FY): 2016-06-30 – 2021-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥93,470,000 (Direct Cost: ¥71,900,000、Indirect Cost: ¥21,570,000); Fiscal Year 2020: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2019: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2018: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2017: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2016: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
• Keywords: 光合成 / 葉緑体 / 電子伝達 / プロトン / サイクリック電子伝達 / プロトン駆動力 / KEA3 / Flv / PTOX / チラコイド膜 / イオンチャネル / プロトン濃度勾配 / イオン輸送 / アンチポーター

Outline of Final Research Achievements

Photosynthetic electron transport is coupled with proton translocation across the thylakoid membrane. Resulting proton concentration gradient is utilized in ATP synthesis and also downregulates electron transport. We clarified the mechanism by which plants optimize the size of delta-pH to avoid photoinhibition without decreasing the rate of photosynthesis. We also tried to artificially modify the regulatory system.

Academic Significance and Societal Importance of the Research Achievements

光は光合成に必須であるが、過剰な光の需要は光合成装置を破壊する。我々は、チラコイド膜を介したプロトンの勾配に着目し、この光の効果の二面性を最適化する分子機構の一部を明らかにした。また積極的に光合成制御を改変するアプローチから、光合成を環境に再最適化することが可能であることを示した。

Research Products

• [Int'l Joint Research] Sanghai Normal University(中国)
• [Int'l Joint Research] CEA Grenoble(フランス)
• [Int'l Joint Research] Padova University(イタリア)
• [Int'l Joint Research] パドバ大学(イタリア)
• [Int'l Joint Research] CEA グルノーブル(フランス)
• [Int'l Joint Research] Imperial College London(英国)
• [Int'l Joint Research] ミュンスター大学(ドイツ)
• [Int'l Joint Research] パドバ大(イタリア)
• [Int'l Joint Research] CEAグルノーブル(フランス)
• [Int'l Joint Research] Imperial College London(英国)
• [Journal Article] Correction to: Flavodiiron proteins enhance the rate of CO2 assimilation in Arabidopsis under fluctuating light intensity (2022)
  • Author(s): Basso Leonardo、Sakoda Kazuma、Kobayashi Ryouhei、Yamori Wataru、Shikanai Toshiharu
  • Journal Title: Plant Physiology Volume: - Issue: 2 Pages: 1173-1173
  • DOI: 10.1093/plphys/kiac129
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] PTOX-dependent safety valve does not oxidize P700 during photosynthetic induction in the Arabidopsis <i>pgr5</i> mutant (2021)
  • Author(s): Zhou Qi、Wang Caijuan、Yamamoto Hiroshi、Shikanai Toshiharu
  • Journal Title: Plant Physiology Volume: 188 Issue: 2 Pages: 1264-1276
  • DOI: 10.1093/plphys/kiab541
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evolution of an assembly factor-based subunit contributed to a novel NDH-PSI supercomplex formation in chloroplasts (2021)
  • Author(s): Kato Yoshinobu、Odahara Masaki、Shikanai Toshiharu
  • Journal Title: Nature Communications Volume: 12 Issue: 1
  • DOI: 10.1038/s41467-021-24065-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Contribution of NDH-dependent cyclic electron transport around photosystem I to the generation of proton motive force in the weak mutant allele of pgr5 (2019)
  • Author(s): Nakano Hiroshi、Yamamoto Hiroshi、Shikanai Toshiharu
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Bioenergetics Volume: 1860 Issue: 5 Pages: 369-374
  • DOI: 10.1016/j.bbabio.2019.03.003
  • Peer Reviewed
• [Journal Article] Evolutionary Model of Plastidial RNA Editing in Angiosperms Presumed from Genome-Wide Analysis of Amborella trichopoda (2019)
  • Author(s): Ishibashi Kota、Small Ian、Shikanai Toshiharu
  • Journal Title: Plant and Cell Physiology Volume: 60 Issue: 10 Pages: 2141-2151
  • DOI: 10.1093/pcp/pcz111
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Modification of Activity of the Thylakoid H+/K+ Antiporter KEA3 Disturbs ?pH-Dependent Regulation of Photosynthesis (2019)
  • Author(s): Wang Caijuan、Shikanai Toshiharu
  • Journal Title: Plant Physiology Volume: 181 Issue: 2 Pages: 762-773
  • DOI: 10.1104/pp.19.00766
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] SPL7 locally regulates copper-homeostasis-related genes in Arabidopsis (2018)
  • Author(s): Araki Ryoichi、Mermod M?lanie、Yamasaki Hiroaki、Kamiya Takehiro、Fujiwara Toru、Shikanai Toshiharu
  • Journal Title: Journal of Plant Physiology Volume: 224-225 Pages: 137-143
  • DOI: 10.1016/j.jplph.2018.03.014
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] PROTON GRADIENT REGULATION 5 contributes to ferredoxin-dependent cyclic phosphorylation in ruptured chloroplasts (2018)
  • Author(s): Wang Caijuan、Takahashi Hiroko、Shikanai Toshiharu
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Bioenergetics Volume: 1859 Issue: 10 Pages: 1173-1179
  • DOI: 10.1016/j.bbabio.2018.07.011
  • Peer Reviewed
• [Journal Article] Stepwise evolution of supercomplex formation with photosystem I is required for stabilization of chloroplast NADH dehydrogenase-like complex: Lhca5-dependent supercomplex formation in Physcomitrella patens (2018)
  • Author(s): Kato Yoshinobu、Odahara Masaki、Fukao Yoichiro、Shikanai Toshiharu
  • Journal Title: The Plant Journal Volume: 96 Issue: 5 Pages: 937-948
  • DOI: 10.1111/tpj.14080
  • Peer Reviewed
• [Journal Article] PGR5-Dependent Cyclic Electron Flow Protects Photosystem I under Fluctuating Light at Donor and Acceptor Sides (2018)
  • Author(s): Yamamoto Hiroshi、Shikanai Toshiharu
  • Journal Title: Plant Physiology Volume: 179 Issue: 2 Pages: 588-600
  • DOI: 10.1104/pp.18.01343
  • Peer Reviewed
• [Journal Article] Specific substitutions of light-harvesting complex I proteins associated with photosystem I are required for supercomplex formation with chloroplast NADH dehydrogenase-like complex (2018)
  • Author(s): Otani Takuto、Kato Yoshinobu、Shikanai Toshiharu
  • Journal Title: The Plant Journal Volume: 94 Issue: 1 Pages: 122-130
  • DOI: 10.1111/tpj.13846
  • Peer Reviewed
• [Journal Article] NDH-PSI Supercomplex Assembly Precedes Full Assembly of the NDH Complex in Chloroplast (2017)
  • Author(s): Kato Yoshinobu、Sugimoto Kazuhiko、Shikanai Toshiharu
  • Journal Title: Plant Physiology Volume: 176 Issue: 2 Pages: 1728-1738
  • DOI: 10.1104/pp.17.01120
  • Peer Reviewed
• [Journal Article] Fine-tuned regulation of the K+/H+antiporter KEA3 is required to optimize photosynthesis during induction (2017)
  • Author(s): Wang Caijuan、Yamamoto Hiroshi、Narumiya Fumika、Munekage Yuri Nakajima、Finazzi Giovanni、Szabo Ildiko、Shikanai Toshiharu
  • Journal Title: Plant Journal Volume: 89 Issue: 3 Pages: 540-553
  • DOI: 10.1111/tpj.13405
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Stromal loop of Lhca6 is responsible for the linker function required for the NDH-PSI supercomplex formation. (2017)
  • Author(s): Takuto Otani, Hiroshi Yamamoto and Toshiharu Shikanai
  • Journal Title: Plant and Cell Physiology Volume: 58 Issue: 4 Pages: 851-861
  • DOI: 10.1093/pcp/pcx009
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Alternative electron transport mediated by flavodiiron proteins is operational in organisms from cyanobacteria up to gymnosperms (2017)
  • Author(s): Il?k Petr、Pavlovi? Andrej、Kou?il Roman、Alboresi Alessandro、Morosinotto Tomas、Allahverdiyeva Yagut、Aro Eva-Mari、Yamamoto Hiroshi、Shikanai Toshiharu
  • Journal Title: New Phytologist Volume: 214 Issue: 3 Pages: 967-972
  • DOI: 10.1111/nph.14536
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Grana-localized proteins, RIQ1 and RIQ2, affect the organization of light-harvesting vomplex II and grana stacking in Arabidopsis. (2016)
  • Author(s): Ryo Yokoyama, Hiroshi Yamamotoa, Maki Kondo, Satomi Takeda, Kentaro Ifuku, Yoichiro Fukao, Yasuhiro Kamei, Mikio Nishimura,Toshiharu Shikanai
  • Journal Title: Plant Cell Volume: 28 Issue: 9 Pages: 2261-2275
  • DOI: 10.1105/tpc.16.00296
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Regulatory network of proton motive force in chloroplasts (2019)
  • Author(s): Toshiharu Shikanai
  • Organizer: ICAR 2019
  • Int'l Joint Research / Invited
• [Presentation] The chloroplast NDH complex: Evolution of assembly ad super complex formation with PSI (2019)
  • Author(s): Toshiharu Shikanai
  • Organizer: 1st Japan-US Binational Meeting
  • Int'l Joint Research / Invited
• [Presentation] Structure and assembly of the chloroplast NDH-PSI supercomplex (2018)
  • Author(s): Toshiharu Shikanai
  • Organizer: The International Conference on Arabidopsis Research
  • Int'l Joint Research / Invited
• [Presentation] Structure, assembly and physiological function of the chloroplast NDH-PSI supercompl (2018)
  • Author(s): Tohsiharu Shikanai
  • Organizer: 1st Asia-Oceania International Congress on Photosynthesis
  • Int'l Joint Research / Invited
• [Presentation] Machinery for the regulation of proton motive force in chloroplasts (2017)
  • Author(s): Tohsiharu Shikanai
  • Organizer: Chloroplast Metabolism and Photosynthesis Meeting, Neuchatel
  • Int'l Joint Research / Invited
• [Presentation] Regulation of proton motive force by alternative electron transport (2017)
  • Author(s): Tohsiharu Shikanai
  • Organizer: SEB GOTHEBURG 2017
  • Int'l Joint Research / Invited
• [Presentation] Regulation of photosynthesis via proton motive force (2017)
  • Author(s): Tohsiharu Shikanai
  • Organizer: IBC2017
  • Int'l Joint Research / Invited
• [Presentation] Molecular mechanisms for optimizing photosynthesis under fluctuating light (2017)
  • Author(s): Tohsiharu Shikanai
  • Organizer: Taiwan-Japan Plant Biology 2017
  • Int'l Joint Research / Invited
• [Presentation] Regulation of photosynthetic electron transport via proton motive force (2017)
  • Author(s): Tohsiharu Shikanai
  • Organizer: 8th Asia and Oceania Conference on Photobiology 2017
  • Int'l Joint Research / Invited
• [Presentation] Regulation of proton motive force in photosynthesis (2016)
  • Author(s): Toshiharu Shikanai
  • Organizer: 17th International Congress on Photosynthesis Research, Maastrichit, Netherland
  • Int'l Joint Research / Invited
• [Presentation] Thylakoid membrane structure and PSII dynamics (2016)
  • Author(s): Ryo Yokoyama, Toshiharu Shikanai
  • Organizer: Finish-Japanese Symposium 2016, Finland
  • Int'l Joint Research / Invited
• [Funded Workshop] 第３回国際ワークショップ（Himadri Pakrasi博士） (2018)