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Intracellular metabolism control of the proton motive force

Planned Research

Project AreaNew Photosynthesis : Reoptimization of the solar energy conversion system
Project/Area Number 16H06559
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionOsaka University

Principal Investigator

Shimizu Hiroshi  大阪大学, 情報科学研究科, 教授 (00226250)

Co-Investigator(Kenkyū-buntansha) 小山内 崇  明治大学, 農学部, 専任准教授 (60512316)
Project Period (FY) 2016-06-30 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥104,650,000 (Direct Cost: ¥80,500,000、Indirect Cost: ¥24,150,000)
Fiscal Year 2020: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
Fiscal Year 2019: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2018: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000)
Fiscal Year 2017: ¥22,490,000 (Direct Cost: ¥17,300,000、Indirect Cost: ¥5,190,000)
Fiscal Year 2016: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Keywordsゲノムスケールモデル / システム生物学 / 光合成 / シミュレーション / 代謝フラックス解析 / ゲノムスケール代謝モデル / システムバイオテクノロジー / システムバイオロジー
Outline of Final Research Achievements

In this study, to understand the regulation mechanism of photosynthesis and metabolism of photosynthetic organisms, a genome scale metabolic reaction model of cyanobacteria (GMM) was reconstructed, including detailed photosynthesis-related reactions. Electron transfer of photosynthesis and metabolic fluxes under different light environmental conditions were simulated and confirmed by experiments. Linear and cyclic electron transfer fluxes were accurately simulated by taking into account of information of excitation ratio of photosynthesis II (PSII) and photosynthesis I (PSI). By integrating simulation results with experimental data of multiple analyses of transcriptome, proteome, metabolome, and metabolic fluxes, re-optimization strategies of photosynthesis and metabolism were discussed.

Academic Significance and Societal Importance of the Research Achievements

食糧やエネルギー問題を解決するため、植物や藻類の「光合成の改良」は重要な課題である。従来の研究では、光合成能力を高めることにのみ重点が置かれてきたが、最近の研究により「光エネルギーの効率的利用」と「過剰光からの防御」のバランスの調節の重要性に関する理解が進められてきた。本研究においては、光合成と代謝をシステム的に統合解析を行うことで、光合成の再最適化に向けた戦略の道筋を示すところに新規性がある。この研究により、二酸化炭素排出や地球温暖化を抑制していくための光合成利用に関する基盤が築かれるとともに光合成システムバイオロジーという新たな学術分野が拓かれたと考えられる。

Report

(6 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (147 results)

All 2021 2020 2019 2018 2017 2016 Other

All Journal Article (42 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 41 results,  Open Access: 18 results,  Acknowledgement Compliant: 7 results) Presentation (97 results) (of which Int'l Joint Research: 18 results,  Invited: 22 results) Book (1 results) Remarks (7 results)

  • [Journal Article] Mutations in hik26 and slr1916 lead to high-light stress tolerance in Synechocystis sp. PCC68032021

    • Author(s)
      Yoshikawa Katsunori、Ogawa Kenichi、Toya Yoshihiro、Akimoto Seiji、Matsuda Fumio、Shimizu Hiroshi
    • Journal Title

      Communications Biology

      Volume: 4 Issue: 1

    • DOI

      10.1038/s42003-021-01875-y

    • NAID

      120007028956

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Thioredoxin pathway in Anabaena sp. PCC 7120: activity of NADPH-thioredoxin reductase C2021

    • Author(s)
      Deschoenmaeker Frederic、Mihara Shoko、Niwa Tatsuya、Taguchi Hideki、Wakabayashi Ken-Ichi、Toyoshima Masakazu、Shimizu Hiroshi、Hisabori Toru
    • Journal Title

      The Journal of Biochemistry

      Volume: - Issue: 6 Pages: 709-719

    • DOI

      10.1093/jb/mvab014

    • NAID

      40022687787

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Role of type I NADH dehydrogenase in Synechocystis sp. PCC 6803 under phycobilisome excited red light2021

    • Author(s)
      Toyoshima Masakazu、Yamamoto Chiaki、Ueno Yoshifumi、Toya Yoshihiro、Akimoto Seiji、Shimizu Hiroshi
    • Journal Title

      Plant Science

      Volume: 304 Pages: 110798-110798

    • DOI

      10.1016/j.plantsci.2020.110798

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Estimation of linear and cyclic electron flows in photosynthesis based on 13C-metabolic flux analysis2021

    • Author(s)
      Yamamoto Chiaki、Toyoshima Masakazu、Kitamura Sayaka、Ueno Yoshifumi、Akimoto Seiji、Toya Yoshihiro、Shimizu Hiroshi
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 131 Issue: 3 Pages: 277-282

    • DOI

      10.1016/j.jbiosc.2020.11.002

    • NAID

      40022515104

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Four-carbon dicarboxylic acid production through the reductive branch of the open cyanobacterial tricarboxylic acid cycle in Synechocystis sp. PCC 68032021

    • Author(s)
      Iijima Hiroko、Watanabe Atsuko、Sukigara Haruna、Iwazumi Kaori、Shirai Tomokazu、Kondo Akihiko、Osanai Takashi
    • Journal Title

      Metabolic Engineering

      Volume: 65 Pages: 88-98

    • DOI

      10.1016/j.ymben.2021.03.007

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient extraction and preservation of thermotolerant phycocyanins from red alga Cyanidioschyzon merolae2021

    • Author(s)
      Yoshida Chihiro、Murakami Miyabi、Niwa Anna、Takeya Masahiro、Osanai Takashi
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 131 Issue: 2 Pages: 161-167

    • DOI

      10.1016/j.jbiosc.2020.09.021

    • NAID

      40022484428

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel allosteric inhibition of phosphoribulokinase identified by ensemble kinetic modeling of Synechocystis sp. PCC 6803 metabolism2020

    • Author(s)
      Nishiguchi Hiroki、Liao James、Shimizu Hiroshi、Matsuda Fumio
    • Journal Title

      Metabolic Engineering Communications

      Volume: 11 Pages: e00153-e00153

    • DOI

      10.1016/j.mec.2020.e00153

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Assessment of Protein Content and Phosphorylation Level in Synechocystis sp. PCC 6803 under Various Growth Conditions Using Quantitative Phosphoproteomic Analysis2020

    • Author(s)
      Toyoshima Masakazu、Tokumaru Yuma、Matsuda Fumio、Shimizu Hiroshi
    • Journal Title

      Molecules

      Volume: 25 Issue: 16 Pages: 3582-3582

    • DOI

      10.3390/molecules25163582

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reconstitution of oxaloacetate metabolism in the tricarboxylic acid cycle in Synechocystis sp. PCC 6803: discovery of important factors that directly affect the conversion of oxaloacetate2020

    • Author(s)
      Ito Shoki、Hakamada Takumi、Ogino Tatsumi、Osanai Takashi
    • Journal Title

      The Plant Journal

      Volume: 105 Issue: 6 Pages: 1449-1458

    • DOI

      10.1111/tpj.15120

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of pH on metabolite excretion and cell morphology of Euglena gracilis under dark, anaerobic conditions2020

    • Author(s)
      Yoshioka Kazumasa、Suzuki Kengo、Osanai Takashi
    • Journal Title

      Algal Research

      Volume: 51 Pages: 102084-102084

    • DOI

      10.1016/j.algal.2020.102084

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fumarase From Cyanidioschyzon merolae Stably Shows High Catalytic Activity for Fumarate Hydration Under High Temperature Conditions2020

    • Author(s)
      Ito Shoki、Iwazumi Kaori、Sukigara Haruna、Osanai Takashi
    • Journal Title

      Frontiers in Microbiology

      Volume: 11 Pages: 2190-2190

    • DOI

      10.3389/fmicb.2020.560894

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Unconventional biochemical regulation of the oxidative pentose phosphate pathway in the model cyanobacterium Synechocystis sp. PCC 68032020

    • Author(s)
      Ito Shoki、Osanai Takashi
    • Journal Title

      Biochemical Journal

      Volume: 477 Issue: 7 Pages: 1309-1321

    • DOI

      10.1042/bcj20200038

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous increases in the levels of compatible solutes by cost‐effective cultivation of Synechocystis sp. PCC 68032020

    • Author(s)
      Iijima Hiroko、Watanabe Atsuko、Sukigara Haruna、Shirai Tomokazu、Kondo Akihiko、Osanai Takashi
    • Journal Title

      Biotechnology and Bioengineering

      Volume: - Issue: 6 Pages: 1649-1660

    • DOI

      10.1002/bit.27324

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of the response regulator <i>rpaA</i> causes an impaired cell division in the Cyanobacterium <i>Synechocystis</i> sp. PCC 68032020

    • Author(s)
      Kizawa Ayumi、Osanai Takashi
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 66 Issue: 2 Pages: 121-128

    • DOI

      10.2323/jgam.2020.01.004

    • NAID

      130007855520

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Flux balance analysis of cyanobacteria reveals selective use of photosynthetic electron transport components under different spectral light conditions2020

    • Author(s)
      Toyoshima Masakazu、Toya Yoshihiro、Shimizu Hiroshi
    • Journal Title

      Photosynthesis Research

      Volume: 143 Issue: 1 Pages: 31-43

    • DOI

      10.1007/s11120-019-00678-x

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-yielding rice Takanari has superior photosynthetic response to a commercial rice Koshihikari under fluctuating light2019

    • Author(s)
      Adachi Shunsuke、Tanaka Yu、Miyagi Atsuko、Kashima Makoto、Tezuka Ayumi、Toya Yoshihiro、Kobayashi Shunzo、Ohkubo Satoshi、Shimizu Hiroshi、Kawai-Yamada Maki、Sage Rowan F、Nagano Atsushi J、Yamori Wataru
    • Journal Title

      Journal of Experimental Botany

      Volume: 70 Issue: 19 Pages: 5287-5297

    • DOI

      10.1093/jxb/erz304

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Biochemical characterisation of fumarase C from a unicellular cyanobacterium demonstrating its substrate affinity, altered by an amino acid substitution2019

    • Author(s)
      Katayama Noriaki、Takeya Masahiro、Osanai Takashi
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 10629-10629

    • DOI

      10.1038/s41598-019-47025-7

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Increased polyhydroxybutyrate levels by ntcA overexpression in Synechocystis sp. PCC 68032019

    • Author(s)
      Arisaka Satomi、Terahara Nodoka、Oikawa Akira、Osanai Takashi
    • Journal Title

      Algal Research

      Volume: 41 Pages: 101565-101565

    • DOI

      10.1016/j.algal.2019.101565

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Citrate synthase from Synechocystis is a distinct class of bacterial citrate synthase2019

    • Author(s)
      Ito Shoki、Koyama Naoto、Osanai Takashi
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 6038-6038

    • DOI

      10.1038/s41598-019-42659-z

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Time-resolved analysis of short term metabolic adaptation at dark transition in Synechocystis sp. PCC68032019

    • Author(s)
      Maruyama Masaharu、Nishiguchi Hiroki、Toyohima Masakazu、Okahashi Nobuyuki、Matsuda Fumio、Shimizu Hiroshi
    • Journal Title

      Journal of Bioscience Bioengineering

      Volume: -

    • NAID

      40022037144

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transomics data-driven, ensemble kinetic modeling for system-level understanding and engineering of the cyanobacteria central metabolism2019

    • Author(s)
      Nishiguchi Hiroki、Hiasa Natsuki、Uebayashi Kiyoka、Liao James、Shimizu Hiroshi、Matsuda Fumio
    • Journal Title

      Metabolic Engineering

      Volume: 52 Pages: 273-283

    • DOI

      10.1016/j.ymben.2019.01.004

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Targeted proteome analysis of microalgae under high-light conditions by optimized protein extraction of photosynthetic organisms.2019

    • Author(s)
      Toyoshima, M., Sakata, M., Ohnishi, K., Tokumaru, Y., Kato, Y., Tokutsu, R., Sakamoto, W., Minagawa, J., Matsuda, F., Shimizu, H.
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 127 Issue: 3 Pages: 394-402

    • DOI

      10.1016/j.jbiosc.2018.09.001

    • NAID

      40021846292

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Amino acid excretion from Euglena gracilis cells in dark and anaerobic conditions2019

    • Author(s)
      Tomita Yuko、Takeya Masahiro、Suzuki Kengo、Nitta Nobuko、Higuchi Chieko、Marukawa-Hashimoto Yuka、Osanai Takashi
    • Journal Title

      Algal Research

      Volume: 37 Pages: 169-177

    • DOI

      10.1016/j.algal.2018.11.017

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptome analysis of the cyanobacterium Synechocystis sp. PCC 6803 and mechanisms of photoinhibition tolerance under extreme high light conditions2018

    • Author(s)
      Ogawa Kenichi、Yoshikawa Katsunori、Matsuda Fumio、Toya Yoshihiro、Shimizu Hiroshi
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 126 Issue: 5 Pages: 596-602

    • DOI

      10.1016/j.jbiosc.2018.05.015

    • NAID

      40021726334

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative Targeted Proteomics of the Central Metabolism and Photosystems in SigE Mutant Strains of Synechocystis sp. PCC 68032018

    • Author(s)
      Tokumaru Yuma、Uebayashi Kiyoka、Toyoshima Masakazu、Osanai Takashi、Matsuda Fumio、Shimizu Hiroshi
    • Journal Title

      Molecules

      Volume: 23 Issue: 5 Pages: 1051-1051

    • DOI

      10.3390/molecules23051051

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Metabolic flux of the oxidative pentose phosphate pathway under low light conditions in Synechocystis sp. PCC 68032018

    • Author(s)
      Ueda Kentaro、Nakajima Tsubasa、Yoshikawa Katsunori、Toya Yoshihiro、Matsuda Fumio、Shimizu Hiroshi
    • Journal Title

      J. Biosci. Bioeng.

      Volume: - Issue: 1 Pages: 38-43

    • DOI

      10.1016/j.jbiosc.2018.01.020

    • NAID

      40021601004

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic manipulation to overexpress rpaA altered photosynthetic electron transport in Synechocystis sp. PCC 68032018

    • Author(s)
      Arisaka Satomi、Sukigara Haruna、Osanai Takashi
    • Journal Title

      J. Biosci. Bioeng.

      Volume: - Issue: 2 Pages: 139-144

    • DOI

      10.1016/j.jbiosc.2018.02.005

    • NAID

      40021645297

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification and Characterisation of Malate Dehydrogenase From Synechocystis sp. PCC 6803: Biochemical Barrier of the Oxidative Tricarboxylic Acid Cycle2018

    • Author(s)
      Takeya Masahiro、Ito Shoki、Sukigara Haruna、Osanai Takashi
    • Journal Title

      Frontiers in Plant Science

      Volume: 9 Pages: 947-947

    • DOI

      10.3389/fpls.2018.00947

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Single Amino Acid Change in 6-Phosphogluconate Dehydrogenase from Synechocystis Conveys Higher Affinity for NADP+ and Altered Mode of Inhibition by NADPH2018

    • Author(s)
      Ito Shoki、Osanai Takashi
    • Journal Title

      Plant and Cell Physiology

      Volume: 12 Pages: 2461-2461

    • DOI

      10.1093/pcp/pcy165

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of Bioplastic Compounds by Genetically Manipulated and Metabolic Engineered Cyanobacteria2018

    • Author(s)
      Katayama Noriaki、Iijima Hiroko、Osanai Takashi
    • Journal Title

      Advances in Experimental Medicine and Biology

      Volume: 1080 Pages: 155-169

    • DOI

      10.1007/978-981-13-0854-3_7

    • ISBN
      9789811308536, 9789811308543
    • Related Report
      2018 Annual Research Report
  • [Journal Article] Cluster-Level Relationships of Genes Involved in Carbon Metabolism in Synechocystis sp. PCC 6803: Development of a Novel Succinate-Producing Strain2018

    • Author(s)
      Takeya Masahiro、Iijima Hiroko、Sukigara Haruna、Osanai Takashi
    • Journal Title

      Plant Cell Physiol.

      Volume: 59 Issue: 1 Pages: 72-81

    • DOI

      10.1093/pcp/pcx162

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of alcohol stress tolerance genes of Synechocystis sp. PCC 6803 using adaptive laboratory evolution2017

    • Author(s)
      Matsusako Takuya、Toya Yoshihiro、Yoshikawa Katsunori、Shimizu Hiroshi
    • Journal Title

      Biotechnology for Biofuels

      Volume: 10 Issue: 1 Pages: 307-307

    • DOI

      10.1186/s13068-017-0996-5

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Substrate Specificity and Allosteric Regulation of a d-Lactate Dehydrogenase from a Unicellular Cyanobacterium are Altered by an Amino Acid Substitution2017

    • Author(s)
      Ito Shoki、Takeya Masahiro、Osanai Takashi
    • Journal Title

      Sci. Rep.

      Volume: 7 Issue: 1 Pages: 15052-15052

    • DOI

      10.1038/s41598-017-15341-5

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Incubation of cyanobacteria under dark, anaerobic conditions and quantification of the excreted Organic acids by HPLC.2017

    • Author(s)
      Yasuda Chika、Iijima Hiroko、Sukigara Haruna、Osanai Takashi
    • Journal Title

      Bio-protocol

      Volume: 7 Issue: 9

    • DOI

      10.21769/bioprotoc.2257

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ACR11 is an activator of plastid-type glutamine synthetase GS2 in Arabidopsis thaliana2017

    • Author(s)
      Takashi Osanai, Ayuko Kuwahara, Hitomi Otsuki, Kazuki Saito, Masami Yokota Hirai
    • Journal Title

      Plant & Cell Physiology

      Volume: 58 Issue: 4 Pages: 650-657

    • DOI

      10.1093/pcp/pcx033

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Metabolic engineering of Synechocystis sp. PCC 6803 for enhanced ethanol production based on flux balance analysis2017

    • Author(s)
      Katsunori Yoshikawa, Yoshihiro Toya, Hiroshi Shimizu
    • Journal Title

      Bioprocess and Biosystems Engineering

      Volume: 40 Issue: 5 Pages: 1-6

    • DOI

      10.1007/s00449-017-1744-8

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Editorial: biotechnology of microalgae, based on molecular biology and biochemistry of eukaryotic algae and cyanobacteria2017

    • Author(s)
      Takashi Osanai, Youn-Il Park, Yuki Nakamura
    • Journal Title

      Frontiers in Microbiology

      Volume: 8 Pages: 118-118

    • DOI

      10.3389/fmicb.2017.00118

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Allosteric inhibition of phosphoenolpyruvate carboxylases is determined by a single amino acid residue in cyanobacteria2017

    • Author(s)
      Masahiko Takeya, Masami Yokota Hirai, Takashi Osanai
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 41080-41080

    • DOI

      10.1038/srep41080

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Metabolic flux analysis of Synechocystis sp. PCC6803 ΔnrtABCD mutant reveals a mechanism for metabolic adaptation to nitrogen-limited conditions2016

    • Author(s)
      Tsubasa Nakajima, Katsunori Yoshikawa, Yoshihiro Toya, Fumio Matsuda, and Hiroshi Shimizu
    • Journal Title

      Plant and Cell Physiology

      Volume: 58 Issue: 5 Pages: 537-545

    • DOI

      10.1093/pcp/pcx047

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Combinatorial deletions of glgC and phaCE enhance ethanol production in Synechocystis sp. PCC 68032016

    • Author(s)
      Katsunori Namakoshi, Tubasa Nakajima, Katsunori Yoshikawa, Yoshihiro Toya, Hiroshi Shimizu
    • Journal Title

      Journal of Biotechnology

      Volume: 239 Pages: 13-19

    • DOI

      10.1016/j.jbiotec.2016.09.016

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Succinate and lactate production from Euglena gracilis during dark, anaerobic conditions2016

    • Author(s)
      Yuko Tomita, Kazumasa Yoshioka, Hiroko Iijima, Ayaka Nakashima, Osamu Iwata, Kengo Suzuki, Tomohisa Hasunuma, Akihiko Kondo, Masami Yokota Hirai, Takashi Osanai
    • Journal Title

      Frontiers in Microbiology

      Volume: 7 Pages: 2050-2050

    • DOI

      10.3389/fmicb.2016.02050

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Anionic metabolite biosynthesis enhanced by potassium under dark, anaerobic conditions in cyanobacteria2016

    • Author(s)
      Sakiko Ueda, Yuhki Kawamura, Hiroko Iijima, Mitsuharu Nakajima, Tomokazu Shirai, Mami Okamoto, Akihiko Kondo, Masami Yokota Hirai, Takashi Osanai
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 32354-32354

    • DOI

      10.1038/srep32354

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] FBA シミュレーションによるシアノバクテリアにおける PSI/PSII励起比と光合成電子伝達フラックスの関係ならびに NDH-1 の機能解析2021

    • Author(s)
      豊島正和、山本千晶、植野嘉文、戸谷吉博、秋本誠志、清水浩
    • Organizer
      第6回光合成細菌ワークショップ
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 光合成生物のシステムバイオロジー2021

    • Author(s)
      清水浩
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Synechocystis sp. PCC 6803のΔndhD1/D2株を用いたレドックスバランス感知フィコビリソーム分解制御機構の解析2021

    • Author(s)
      豊島正和、阪田真澄、山本千晶、植野嘉文、戸谷吉博、秋本誠志、清水浩
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] NAD(P)H dehydrogenase deletion enhances accumulation of polyhydroxybutyrate in Synechocystis sp. PCC 68032021

    • Author(s)
      Masakazu Yajima, Masakazu Toyoshima, Yoshihiro Toya, Hiroshi Shimizu
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 動的代謝モデルを用いたカルビン回路の代謝制御機構の解析2021

    • Author(s)
      田島 遼太郎、池原 万由、戸谷 吉博、清水 浩
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Organic acid excretion from cyanobacteria during fermentation2020

    • Author(s)
      Takashi Osanai
    • Organizer
      Genetic Modification Tools in Cyanobacteria and Microalgae
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 13C-metabolic flux analysis reveals photosynthetic cofactor production rates in cyanobacteria2020

    • Author(s)
      Chiaki Yamamoto、 Sayaka Kitamura、Masakazu Toyoshima、Yoshihiro Toya、 Hiroshi Shimizu
    • Organizer
      上海交通大学学術交流セミナー
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 異なる波長の光照射下におけるシアノバクテリアの光合成と代謝のシミュレーション予測による適応機構の解析2020

    • Author(s)
      豊島正和、山本千晶、植野嘉文、戸谷吉博、秋本誠志、清水浩
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] FBAシミュレーションによる異なる波長の光照射下におけるSynechocystis sp. PCC 6803の光合成電子伝達経路の選択的使用の解明2020

    • Author(s)
      豊島正和、山本千晶、植野嘉文、 戸谷吉博、秋本誠志、清水浩
    • Organizer
      日本植物生理学会2020年度大阪年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 13C代謝フラックス解析を用いたシアノバクテリアにおける光化学系からのATP/NADPH生産速度の推定2020

    • Author(s)
      山本千晶、北村さや香、豊島正和、植野嘉文、戸谷吉博、秋本誠志、清水浩
    • Organizer
      日本植物生理学会2020年度大阪年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 化学量論モデルを用いたFBAシミュレーションによるシアノバクテリアの光合成電子伝達の解析2020

    • Author(s)
      豊島正和、山本千晶、 植野嘉文、 戸谷吉博、秋本誠志、 清水浩
    • Organizer
      第5回光合成細菌ワークショップ
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] シアノバクテリアのカルビン回路の反応速度論モデルの開発2020

    • Author(s)
      田島遼太郎、池原万由、戸谷吉博、清水浩
    • Organizer
      化学工学会第85年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] in silico analysis and design of photosynthesis and metabolic pathways of Synechocystis sp. PCC68032019

    • Author(s)
      Shimizu Hiroshi
    • Organizer
      日米二国間セミナー
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] FBA simulation of photosynthetic electron flow of Synechocystis sp. PCC 6803 under different spectral light condition2019

    • Author(s)
      Toyoshima Masakazu、Yamamoto Chiaki 、Ueno Yoshifumi、Toya Yoshihiro 、Akimoto Seiji,、Shimizu Hiroshi
    • Organizer
      日米二国間セミナー
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Metabolic Engineering of Synechocystis sp. PCC68032019

    • Author(s)
      Shimizu Hiroshi、Toyoshima Masakazu、Toya Yoshihiro、Matsuda Fumio
    • Organizer
      APCChE 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Metabolic engineering of cyanobacteria and eukaryotic algae based on biochemistry and molecular biology2019

    • Author(s)
      Osanai Takashi
    • Organizer
      Cyano2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] FBAによるシアノバクテリアの光合成電子伝達フラックス状態の解析2019

    • Author(s)
      豊島正和、山本千晶、植野嘉文、戸谷吉博、秋本誠志、清水浩
    • Organizer
      令和元年度秋季新光合成領域会議
    • Related Report
      2019 Annual Research Report
  • [Presentation] 13C代謝フラックス解析によるシアノバクテリアにおける光化学反応の補酵素生産速度の推定2019

    • Author(s)
      山本千晶、北村さや香、豊島正和、戸谷吉博、清水浩
    • Organizer
      令和元年度秋季新光合成領域会議
    • Related Report
      2019 Annual Research Report
  • [Presentation] 異なる波長の光照射下におけるSynechocystis sp. PCC 6803の網羅的定量プロテオームと絶対定量2019

    • Author(s)
      豊島正和、阪田真澄、大西一輝、戸谷吉博、松田史生、清水浩
    • Organizer
      藍藻の分子生物学2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] FBAシミュレーションによる単波長光照射下におけるシアノバクテリアの光合成電子伝達経路のフラックス状態の解析2019

    • Author(s)
      豊島正和、山本千晶、戸谷吉博、清水浩
    • Organizer
      第71回日本生物工学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 13C代謝フラックス解析に基づくシアノバクテリアの単波長光照射下に対する応答解析2019

    • Author(s)
      山本千晶、北村さや香、豊島正和、戸谷吉博、清水浩
    • Organizer
      第71回日本生物工学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] QconCAT法を用いたシアノバクテリア光合成装置の絶対定量に関する研究2019

    • Author(s)
      豊島正和、大西一輝、阪田真澄、戸谷吉博、清水浩
    • Organizer
      新光合成、光合成若手の会ジョイント若手ワークショップ
    • Related Report
      2019 Annual Research Report
  • [Presentation] FBAシミュレーションによる異なるPSI/PSII励起比におけるシアノバクテリアの光合成電子伝達の解析2019

    • Author(s)
      豊島正和、山本千晶、戸谷吉博、清水浩
    • Organizer
      2019年度ラン藻ゲノム交流会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] プロトン駆動力による細胞内代謝制御2019

    • Author(s)
      清水浩
    • Organizer
      令和元年度春季新光合成領域会議
    • Related Report
      2019 Annual Research Report
  • [Presentation] 異なるPSI/PSII励起比における光合成電子伝達並びに呼吸鎖のフラックスバランス解析2019

    • Author(s)
      豊島正和、戸谷吉博、清水浩
    • Organizer
      令和元年度春季新光合成領域会議
    • Related Report
      2019 Annual Research Report
  • [Presentation] 異なる波長の光照射下におけるシアノバクテリアの中心代謝の13C代謝フラックス解析2019

    • Author(s)
      山本千晶、北村さやか、豊島正和、戸谷吉博、清水浩
    • Organizer
      令和元年度春季新光合成領域会議
    • Related Report
      2019 Annual Research Report
  • [Presentation] FBAによる単波長光照射下におけるシアノバクテリアの光合成電子伝達と代謝のシミュレーション2019

    • Author(s)
      豊島正和、戸谷吉博、清水浩
    • Organizer
      第10回日本光合成学会年会およびシンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] シアノバクテリアのクエン酸回路を基盤としたバイオプラ原料の生産2019

    • Author(s)
      小山内崇
    • Organizer
      第71回日本生物工学会大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 異なる波長の光照射下におけるSynechocystis sp. PCC 6803の網羅的定量プロテオーム解析2019

    • Author(s)
      豊島正和、阪田真澄、戸谷吉博、松田史生、清水浩
    • Organizer
      第60回植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 異なる波長の光照射下におけるシアノバクテリアの中心代謝の13C代謝フラックス解析2019

    • Author(s)
      山本千晶、北村さやか、豊島正和、戸谷吉博、清水浩
    • Organizer
      第61回植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Systems Biology and Metabolic Engineering of Photosynthetic Organisms2018

    • Author(s)
      Shimizu Hiroshi
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Biogenesis 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Relationship between PSI/PSII excitation ratio and photosynthetic electron transport of Synechocystis sp. PCC 6803 by FBA simulation2018

    • Author(s)
      Toyoshima Masakazu、Toya Yoshihiro、Shimizu Hiroshi
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Biogenesis 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rational metabolic engineering of central metabolism in cyanobacteria using kinetic model ensemble2018

    • Author(s)
      Nishiguchi Hiroki、Nagai Hikaru、Matsuda Fumio、Shimizu Hiroshi
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Biogenesis 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Target phosphoproteome analysis of Synchocystis sp PCC 6803 using nano liquid chromatography- triple quadrupole mass spectrometry2018

    • Author(s)
      Tokumaru Yuma、Toyoshima Masakazu、Matsuda Fumio、Shimizu Hiroshi
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Biogenesis 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Dynamic modeling of central metabolism in cyanobacteria for improving ethanol production2018

    • Author(s)
      Nishiguchi Hiroki、Nagai Hikaru、Matsuda Fumio、Shimizu Hiroshi
    • Organizer
      13th KAIST-OSAKA U Symposium 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Target phosphoproteome analysis of Synchocystis sp PCC 6803 using nano liquid chromatography- triple quadrupole mass spectrometry2018

    • Author(s)
      Tokumaru Yuma、Toyoshima Masakazu、Matsuda Fumio、Shimizu Hiroshi
    • Organizer
      13th KAIST-OSAKA U Symposium 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Overview of microalgal biotechnology based on enzymatic analyses2018

    • Author(s)
      Osanai Takashi
    • Organizer
      RIKEN Seminar
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Relationship between increased production of PHB and photosynthesis due to overexpression of ntcA in Synechocystis sp. PCC 68032018

    • Author(s)
      Arisaka Satomi、Terahara Nodoka、Oikawa Akira、Osanai Takashi
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Biogenesis2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シアノバクテリアのゲノムスケール代謝モデルを用いたFBAシミュレーションによる解析2018

    • Author(s)
      豊島正和、戸谷吉博、清水浩
    • Organizer
      第5回分子珪藻研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] FBAシミュレーションによる単波長光照射下におけるPSI/PSII励起比とサイクリック電子伝達の解析2018

    • Author(s)
      豊島正和、戸谷吉博、清水浩
    • Organizer
      平成30年度秋季領域会議
    • Related Report
      2018 Annual Research Report
  • [Presentation] 代謝工学による光合成の統合解析と工学応用2018

    • Author(s)
      清水浩
    • Organizer
      第70回日本生物工学会大会 シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 計算機工学に基づく代謝設計とその有効性の実証に関する研究2018

    • Author(s)
      清水浩
    • Organizer
      第70回日本生物工学会大会 生物工学功績賞受賞講演
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 様々な波長の光照射下におけるシアノバクテリアの解析2018

    • Author(s)
      豊島正和、阪田真澄、戸谷吉博、松田史生、清水浩
    • Organizer
      第70回日本生物工学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 動的代謝モデルアンサンブルを用いたエタノール生産シアノバクテリアの代謝工学2018

    • Author(s)
      西口大貴、永井暉、松田史生、清水浩
    • Organizer
      第70回日本生物工学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シアノバクテリア中心炭素代謝における光量変化時の短期応答の解析2018

    • Author(s)
      丸山正晴、西口大貴、豊島正和、松田史生、清水浩
    • Organizer
      第70回日本生物工学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] プロトン駆動力による細胞内代謝制御2018

    • Author(s)
      清水浩
    • Organizer
      平成30年度春季新光合成領域会議
    • Related Report
      2018 Annual Research Report
  • [Presentation] シアノバクテリアの代謝モデルを利用した動的解析に向けて2018

    • Author(s)
      戸谷吉博、豊島正和、清水浩
    • Organizer
      平成30年度春季新光合成領域会議
    • Related Report
      2018 Annual Research Report
  • [Presentation] FBAを用いた様々な波長の光照射下におけるSynechocystis sp. PCC 6803の野生株とNDH-1欠損株の代謝シミュレーション2018

    • Author(s)
      豊島正和、戸谷吉博、松田史生、清水浩
    • Organizer
      平成30年度春季新光合成領域会議
    • Related Report
      2018 Annual Research Report
  • [Presentation] クラミドモナス光化学系の定量プロテオーム解析とQconCAT法による絶対定量2018

    • Author(s)
      大西一輝、徳丸雄磨、 得津隆太郎、 皆川純、 豊島正和、松田史生、清水浩
    • Organizer
      平成30年度春季新光合成領域会議
    • Related Report
      2018 Annual Research Report
  • [Presentation] 適応進化実験によるシアノバクテリア強光耐性株の獲得とその解析2018

    • Author(s)
      吉川勝徳、小川健一、戸谷吉博、秋本誠志、松田史生、清水浩
    • Organizer
      第9回日本光合成学会年会およびシンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 様々な波長の光照射下のSynechocystis sp. PCC 6803 の野生株とNDH-1欠損株の生育を代謝シミュレーションする2018

    • Author(s)
      豊島正和、戸谷吉博、松田史生、清水浩
    • Organizer
      第9回日本光合成学会年会およびシンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] クラミドモナス光化学系の定量プロテオミクス法の検討と利用2018

    • Author(s)
      大西一輝, 徳丸雄磨, 得津隆太郎, 皆川純, 豊島正和, 松田史生, 清水浩
    • Organizer
      第9回日本光合成学会年会およびシンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] ピルビン酸関連酵素からみるシアノバクテリア炭素代謝の不思議2018

    • Author(s)
      小山内崇
    • Organizer
      マリンバイオテクノロジー学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 微細藻類の遺伝子工学による二酸化炭素からの有用2018

    • Author(s)
      小山内崇
    • Organizer
      高分子討論会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] シアノバクテリアのメタボロミクスと有機酸生産2018

    • Author(s)
      小山内崇
    • Organizer
      メタボロームシンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 嫌気発酵条件におけるバイオコハク酸の細胞外生産2018

    • Author(s)
      小山内崇
    • Organizer
      ユーグレナ研究会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] クラミドモナス光化学系の定量プロテオミクス法の開発2018

    • Author(s)
      大西一輝、徳丸雄磨、得津隆太郎、皆川純、豊島正和、松田史生、清水浩
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光量変化に対するシアノバクテリア代謝応答の解析2018

    • Author(s)
      丸山正晴、西口大貴、豊島正和、松田史生、清水浩
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 安定同位体非定常の13C代謝フラックス解析およびプロテオーム解析によるn-ブタノールを生産するSynechococcus elongatus PCC 7942の代謝状態の推定2018

    • Author(s)
      和田圭介、植林希代加、戸谷吉博、傳寶雄大、Putri Sastia、松田史生、福崎英一郎、James C Liao、清水浩
    • Organizer
      化学工学会第83年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] FBAを用いた様々な波長の光条件下におけるSynechocystis sp. PCC 6803の代謝シミュレーション2018

    • Author(s)
      豊島正和、戸谷吉博、松田史生、清水浩
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 四重極タンデム質量分析装置を用いたSynechocystis sp. PCC 6803のターゲットリン酸化プロテオーム解析2018

    • Author(s)
      徳丸雄磨・豊島正和、松田史生、清水浩
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 13C代謝フラックス解析を用いたSynechocystis sp. PCC 6803のndhF遺伝子破壊株におけるNADPH消費機構の寄与率の解明2018

    • Author(s)
      和田圭介、戸谷吉博、松田史生、清水浩
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シアノバクテリアを用いた二酸化炭素からのバイオコハク酸生産2018

    • Author(s)
      小山内崇
    • Organizer
      日本農芸化学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 微細藻類の発酵による二酸化炭素を出発物質とした有機酸生産2018

    • Author(s)
      小山内崇
    • Organizer
      JBA発酵と代謝研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 光合成微生物の化学量論モデルを利用したシミュレーションとその応用2017

    • Author(s)
      戸谷吉博,吉川勝徳,松田史生,清水浩
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] シグマ因子SigEによるSynechocystis sp. PCC 6803のプロテオームの制御2017

    • Author(s)
      徳丸雄磨, 植林希代加, 小山内崇, 松田史生, 清水浩
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 代謝物濃度の絶対定量値を用いたエタノール生産シアノバクテリア代謝律速点の解析2017

    • Author(s)
      西口大貴, 永井暉, 松田史生, 清水浩
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] シアノバクテリアのシステムバイオロジーによる統合理解と工学応用2017

    • Author(s)
      清水浩、豊島正和、戸谷吉博、松田史生
    • Organizer
      藍藻の分子生物学2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] nanoLC-MSを用いたシアノバクテリアの網羅的定量プロテオーム解析2017

    • Author(s)
      豊島正和、阪田真澄、松田史生、清水浩
    • Organizer
      藍藻の分子生物学2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Chlamydomonas reinhardtiiの定量プロテオミクスのための膜タンパク質可溶化手法の検討2017

    • Author(s)
      大西一輝、徳丸雄磨、阪田真澄、豊島正和、戸谷吉博、松田史生、清水浩
    • Organizer
      科研費新学術領域研究平成29年度秋期領域会議
    • Related Report
      2017 Annual Research Report
  • [Presentation] シアノバクテリアのSigEの代謝制御解明に向けた定量プロテオーム解析 "2017

    • Author(s)
      徳丸雄磨、植林希代加、豊島正和、小山内崇、松田史生、清水浩
    • Organizer
      科研費新学術領域研究平成29年度秋期領域会議
    • Related Report
      2017 Annual Research Report
  • [Presentation] Synechocystis sp. PCC 6803における多様な波長の光環境下での代謝シミュレーション2017

    • Author(s)
      豊島正和、戸谷吉博、松田史生、清水浩
    • Organizer
      科研費新学術領域研究平成29年度秋期領域会議
    • Related Report
      2017 Annual Research Report
  • [Presentation] nanoLC-MSを用いた網羅的定量プロテオーム解析に向けたタンパク質抽出方法の検討2017

    • Author(s)
      豊島正和、阪田真澄、大西一輝、徳丸雄磨、松田史生、清水浩
    • Organizer
      科研費新学術領域研究平成29年度秋期領域会議
    • Related Report
      2017 Annual Research Report
  • [Presentation] ブタノール生産シアノバクテリアの代謝解析2017

    • Author(s)
      和田 圭介、植林 希代加、 戸谷 吉博、傳寶 雄大、Putri Sastia 、松田 史生、福崎 英一郎、 Liao James C ,、清水 浩
    • Organizer
      科研費新学術領域研究平成29年度秋期領域会議
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光量変化に対するシアノバクテリア代謝適応機構の解析2017

    • Author(s)
      丸山正晴・西口大貴、松田史生、清水浩
    • Organizer
      第11回メタボロームシンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 代謝物絶対定量データを用いたシアノバクテリア動的代謝モデル構築2017

    • Author(s)
      西口大貴、永井暉、松田史生、清水浩
    • Organizer
      第11回メタボロームシンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 代謝物濃度絶対定量値を用いたエタノール生産シアノバクテリア代謝律速点の解析2017

    • Author(s)
      西口大貴、永井暉、松田史生、清水浩
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シグマ因子SigEによるシアノバクテリア代謝制御の定量プロテオーム解析2017

    • Author(s)
      徳丸雄磨、植林希代加、小山内崇、松田史生、清水浩
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 13C代謝フラックス解析およびプロテオーム解析を用いたn-ブタノール生産シアノバクテリア株の代謝解析2017

    • Author(s)
      和田圭介、植林希代加、戸谷吉博、傳寶雄大、Putri Sastia、松田史生、福崎 英一郎、Liao James、清水浩
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光合成微生物の代謝モデルの開発と応用2017

    • Author(s)
      豊島正和、戸谷吉博、吉川勝徳、 松田史生、 清水浩
    • Organizer
      化学工学会第49回秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Synechocystis sp. PCC 6803の中心代謝経路と光化学系の制御メカニズムの解析2017

    • Author(s)
      和田圭介、戸谷吉博、吉川勝徳、松田史生、清水浩
    • Organizer
      化学工学会第49回秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シアノバクテリアSynechocystis sp. PCC 6803の光化学反応を精緻化したゲノムスケールモデルを用いた様々な光条件における代謝シミュレーション2017

    • Author(s)
      豊島正和、戸谷吉博、松田史生、清水浩
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Synechocystis sp. PCC 6803の精緻化した代謝モデルを用いた光合成研究への応用2017

    • Author(s)
      豊島正和、戸谷吉博、松田史生、清水浩
    • Organizer
      第10回日本光合成学会年会およびシンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光量条件がシアノバクテリアの代謝フラックスに及ぼす影響の解析2017

    • Author(s)
      上田賢太朗、仲嶋翼、吉川勝徳、戸谷吉博、松田史生、清水浩
    • Organizer
      第9回日本光合成学会年会およびシンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 代謝物濃度絶対定量値を用いたエタノール生産シアノバクテリア代謝律速点の解析2017

    • Author(s)
      西口大貴、永井暉、松田史生、清水浩
    • Organizer
      第65回質量分析総合討論会 2017 つくば
    • Related Report
      2017 Annual Research Report
  • [Presentation] QconCAT法を用いたシアノバクテリア光化学装置の定量プロテオミクス2017

    • Author(s)
      徳丸雄磨、植林希代加、徳山健斗、和田圭介、松田史生、清水浩
    • Organizer
      第65回質量分析総合討論会 2017 つくば
    • Related Report
      2017 Annual Research Report
  • [Presentation] Quantitative proteome analysis of microalgae under high light stress2017

    • Author(s)
      Masakazu Toyoshima, Masumi Sakata, Kazuki Ohnishi, Yuma Tokumaru, Fumio Matsuda, Hiroshi Shimizu
    • Organizer
      I-Bios2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Metabolic engineering of cyanobacteria for bio-succinate production with metabolomic analysis.2017

    • Author(s)
      Takashi Osanai
    • Organizer
      The 8th Asia and Oceania Conference on Photobiology.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ラン藻の代謝工学によるバイオプラスチック原料生産の展開2017

    • Author(s)
      小山内崇
    • Organizer
      JST-CREST/さきがけ領域公開シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 微細藻類を用いたプラスチック原料の生産2017

    • Author(s)
      小山内崇
    • Organizer
      NPO法人地球環境カレッジ
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 微細藻類を用いたプラスチック原料の新規増産法の開発2017

    • Author(s)
      小山内崇
    • Organizer
      第5回バイオ関連化学シンポジウム若手フォーラム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Integration of in silico design and experimental evaluation of metabolic pathways for creation of microbial cell factories,2016

    • Author(s)
      Hiroshi Shimizu
    • Organizer
      International Conference on the Metabolic Science,
    • Place of Presentation
      Nanjiao Hotel, Shanghai China,
    • Year and Date
      2016-10-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 代謝物濃度絶対定量によるシアノバクテリア代謝律速点の解析2016

    • Author(s)
      西口大貴, 永井 暉, 松田 史生, 清水 浩
    • Organizer
      第10回メタボロームシンポジウム
    • Place of Presentation
      鶴岡メタボロームキャンパス(山形県鶴岡市)
    • Year and Date
      2016-10-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] 13C-metabolic flux analysis for rational design of metabolic pathway modification of the industrially useful microorganisms2016

    • Author(s)
      Hiroshi Shimizu
    • Organizer
      The 13th International Symposium on the Genetics of Industrial Microorganisms
    • Place of Presentation
      East Lake Hotel, Wuhan, China
    • Year and Date
      2016-10-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 糖代謝遺伝子の分子系統と発現制御の解析2016

    • Author(s)
      竹屋壮浩, 小山内崇
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] シアノバクテリアの時計関連遺伝子rpaA過剰発現による光合成の改変2016

    • Author(s)
      有坂聡美, 小山内崇
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Euglena gracilisにおける好気 / 嫌気条件下での一次代謝産物の変化2016

    • Author(s)
      冨田結芙子, 小山内崇
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Book] BIOTECHNOLOGY, Wiley-VCH book series, Weinheim, Vol 5: Applied Bioengineering: Innovations and Future Directions, Chapter 7, Omics integrations for the state analysis of microbial processes2017

    • Author(s)
      Hiroshi Shimizu, Chikara Fursawa, Takashi Hirasawa, Katsunori Yoshikawa, Yoshihiro Toya, Tomokazu Shirai, and Fumio Matsuda
    • Total Pages
      36
    • Publisher
      Wiley-VCH
    • Related Report
      2016 Annual Research Report
  • [Remarks] 清水浩研究室

    • URL

      http://www-shimizu.ist.osaka-u.ac.jp/hp/index.html

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report 2018 Annual Research Report 2017 Annual Research Report 2016 Annual Research Report
  • [Remarks] 新光合成 光エネルギーシステムの最適化

    • URL

      http://photosynthesis.nibb.ac.jp/index.htm

    • Related Report
      2020 Annual Research Report
  • [Remarks] 明大農学部農芸化学科環境バイオテクノロジー 研究室

    • URL

      https://osanaimeiji.wixsite.com/website

    • Related Report
      2020 Annual Research Report
  • [Remarks] 新光合成 光エネルギーシステムの最適化

    • URL

      http://photosynthesis.nibb.ac.jp/index.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] 新光合成 光エネルギー変換システムの最適化

    • URL

      http://photosynthesis.nibb.ac.jp/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 新光合成 光エネルギー変換システムの再最適化

    • URL

      http://photosynthesis.nibb.ac.jp/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 新光合成 光エネルギー変換システムの再最適化

    • URL

      http://photosynthesis.nibb.ac.jp/index.html

    • Related Report
      2016 Annual Research Report

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Published: 2016-07-04   Modified: 2022-01-27  

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