| Budget Amount *help |
¥143,520,000 (Direct Cost: ¥110,400,000、Indirect Cost: ¥33,120,000)
Fiscal Year 2021: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2020: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2019: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2018: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2017: ¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000)
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| Outline of Final Research Achievements |
NF-kB transcription factor controls induction of various genes in inflammation and immune response, however, their molecular basis largely remains unclear. In this study, we clarified the functional mechanism of NF-kB by quantitatively analyzing various omics data obtained from next-generation sequences using immune B cells, breast cancer cells, and atopic dermatitis animal models. As a result, it was shown that the chromatin opening in the enhancer and promoter regions and the number of DNA bindings of the NF-kB molecule are important for enhancing the expression of the target gene by NF-kB. It was also shown that NF-kB induces liquid-liquid phase separation (LLPS) -like molecular interactions in the nucleus and increases cellular heterogeneity in gene expression.
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