| Budget Amount *help |
¥127,270,000 (Direct Cost: ¥97,900,000、Indirect Cost: ¥29,370,000)
Fiscal Year 2021: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2020: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2019: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2018: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
Fiscal Year 2017: ¥29,380,000 (Direct Cost: ¥22,600,000、Indirect Cost: ¥6,780,000)
|
|---|
| Outline of Final Research Achievements |
In this project, we aimed to develop highly-sensitive and/or multiplexed epigenome sequencing methods, which should be critical to accelerate trans-omic analyses. First, we developed a highly efficient method for single-stranded DNA ligation termed TACS ligation and introduced it to further improve the performance of PBAT, the most sensitive and reliable methylome sequencing method of our own. Second, we developed DMS-seq for in vivo genome-wide mapping of protein-DNA interactions and nucleosome centers, thus adding a unique method to the toolbox for epigenomics. Third, we applied TACS ligation to cell-free DNA analysis and revealed a novel class of short single-stranded DNA enriched with noncanonical DNA structure, or antisense of G-quadruplex structure. Fourth, we identified a novel DNA methyltransferase, which should lay a basis for a multiplexed epigenomics method to encode histone modification information to methylation of neighborhood DNA.
|
