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Development of the next generation transcriptome analysis and its application

Planned Research

Project AreaTransomic Analysis of Metabolic Adaptation
Project/Area Number 17H06306
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe University of Tokyo

Principal Investigator

Suzuki Yutaka  東京大学, 大学院新領域創成科学研究科, 教授 (40323646)

Project Period (FY) 2017-06-30 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥127,140,000 (Direct Cost: ¥97,800,000、Indirect Cost: ¥29,340,000)
Fiscal Year 2021: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2020: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2019: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2018: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2017: ¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Keywordsトランスクリプトーム / エピゲノム / シングルセル解析 / 空間トランスクリプトーム解析
Outline of Final Research Achievements

In this research, our research group has provided the next-generation sequence analysis platform, also including a series of template preparation platforms, and primary data analysis platforms to all research groups in this research area. At the same time, this research group has also independently developed and practiced the basic technology for elucidating the diversity of metabolic adaptation during drug response of cancer cells. With a particular focus on gene expression regulations, we attempted to increase the efficiency of transcriptome and epigenome analysis and systematically produce data on omics drug perturbation responses. An information analysis model was constructed across each omics hierarchy starting from the acquired data to understand the elastic response of the cancer cells in drug responses.

Academic Significance and Societal Importance of the Research Achievements

本研究期間内に行った次世代シークエンスデータ産生支援は、特に公募班若手研究者との共同研究として成果を論文発表することができた。該当分野になじみのなかった特に若手研究者に対して、大規模オーミクスデータ産生/解析への参入を促すことができたと考えている。独自の課題であるがん細胞を対象とした多層オミクス解析について行ったトランスクリプトーム-エピゲノムネットワークにおける抗がん剤作用モジュールのカタログ化、その1細胞レベルへの計測の精密化を通じ、がん細胞薬剤応答の多様性の解明に向けて出発材料を創出することができたと考えている。

Report

(6 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (24 results)

All 2021 2020 2019 2018 2017 Other

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (14 results) (of which Int'l Joint Research: 4 results,  Invited: 13 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Predictive markers based on transcriptome modules for vinorelbine-based adjuvant chemotherapy for lung adenocarcinoma patients.2021

    • Author(s)
      Nakasone S, Suzuki A, Okazaki H, Onodera K, Zenkoh J, Ishii G, Suzuki Y, Tsuboi M, Tsuchihara K.
    • Journal Title

      Lung Cancer.

      Volume: 158 Pages: 115-125

    • DOI

      10.1016/j.lungcan.2021.06.011

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Aberrant splicing isoforms detected by full-length transcriptome sequencing as transcripts of potential neoantigens in non-small cell lung cancer.2021

    • Author(s)
      Oka M, Xu L, Suzuki T, Yoshikawa T, Sakamoto H, Uemura H, Yoshizawa AC, Suzuki Y, Nakatsura T, Ishihama Y, Suzuki A, Seki M
    • Journal Title

      Genome Biol.

      Volume: 22(1):9 Issue: 1 Pages: 1-9

    • DOI

      10.1186/s13059-020-02240-8

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Essential role of the Crk family-dosage in DiGeorge-like anomaly and metabolic homeostasis.2020

    • Author(s)
      Akira Imamoto, Sewon Ki, Leiming Li, Kazunari Iwamoto, Venkat Maruthamuthu, John Devany, Ocean Lu, Tomomi Kanazawa, Suxiang Zhang, Takuji Yamada, Akiyoshi Hirayama, Shinji Fukuda, Yutaka Suzuki, Mariko Okada
    • Journal Title

      Life Science Alliance

      Volume: - Issue: 2 Pages: e201900635-e201900635

    • DOI

      10.26508/lsa.201900635

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Characterization of cancer omics and drug perturbations in panels of lung cancer cells2019

    • Author(s)
      Suzuki Ayako、Onodera Keiichi、Matsui Ken、Seki Masahide、Esumi Hiroyasu、Soga Tomoyoshi、Sugano Sumio、Kohno Takashi、Suzuki Yutaka、Tsuchihara Katsuya
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 19529-19529

    • DOI

      10.1038/s41598-019-55692-9

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of a genomic enhancer that enforces proper apoptosis induction in thymic negative selection2019

    • Author(s)
      Hojo Miki Arai、Masuda Kyoko、Hojo Hiroaki、Nagahata Yosuke、Yasuda Keiko、Ohara Daiya、Takeuchi Yusuke、Hirota Keiji、Suzuki Yutaka、Kawamoto Hiroshi、Kawaoka Shinpei
    • Journal Title

      Nature Communications

      Volume: 10 Issue: 1 Pages: 2603-2603

    • DOI

      10.1038/s41467-019-10525-1

    • NAID

      120006782209

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ゲノム医療における一細胞解析2018

    • Author(s)
      鹿島幸恵,鈴木絢子,関真秀,鈴木穣
    • Journal Title

      実験医学増刊 動き始めた がんゲノム医療 ;深化と普及のための基礎研究課題

      Volume: Vol.36 No.15 Pages: 2626-2630

    • Related Report
      2018 Annual Research Report
  • [Journal Article] シングルセル解析とナノポアシークエンス2018

    • Author(s)
      関真秀, 鈴木穣
    • Journal Title

      医学のあゆみ 遺伝子解析研究の新時代

      Volume: 266巻5号 Pages: 327-334

    • Related Report
      2018 Annual Research Report
  • [Presentation] Single Cell and Spatial Transcriptome Analyses Reveals Heterogeneity of Cancers2021

    • Author(s)
      Yutaka Suzuki
    • Organizer
      第80回日本癌学会(international session)
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] がんのシングルセル/空間トランスクリプトーム解析2021

    • Author(s)
      鈴木 穣
    • Organizer
      第80回日本癌学会 (教育セッション・がん研究入門コース)
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] ゲノム解析技術の進展~シングルセル/ロングリード解析技術の臨床検体への応用2021

    • Author(s)
      鈴木 穣
    • Organizer
      第18回日本免疫治療学会学術集会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] ゲノム解析技術の展開:クリニカルシークエンスからシングルセル/空間トランスクリプトーム解析へ2021

    • Author(s)
      鈴木 穣
    • Organizer
      第 39 回日本内分泌学会 内分泌代謝学サマーセミナー
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] シングルセル解析技術の進展2020

    • Author(s)
      鈴木穣
    • Organizer
      第19回日本再生医療学会総会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Introduction to Single Cell Analysis2020

    • Author(s)
      Yutaka Suzuki
    • Organizer
      第84回日本循環器学会学術総会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] がんのシングルセルおよび空間トランスクリプトーム解析2020

    • Author(s)
      鈴木穣
    • Organizer
      第79回日本癌学会学術総会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] ゲノム解析技術の進展2020

    • Author(s)
      鈴木 穣
    • Organizer
      共同利用・共同研究拠点「マルチオミックスによる遺伝子発現制御の先端的医学共同研究拠点」シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Single Cell Analysis of Cancer Cells2019

    • Author(s)
      Yutaka Suzuki
    • Organizer
      EMBO Workshop on Single Cell Biology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Single Cell Analysis Reveals the Heterogeneity of Cancer Cells:2019

    • Author(s)
      Yutaka Suzuki
    • Organizer
      The 28th International KOGO Annual Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ゲノム解析新技術:ロングリード解析とシングルセル解析2018

    • Author(s)
      鈴木穣
    • Organizer
      日本内分泌学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] がん細胞株のシングルセル解析:複数プラットフォーム間の横断解析2018

    • Author(s)
      鈴木穣
    • Organizer
      日本分子腫瘍マーカー研究会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Multi-omics analysis of cancer cells2017

    • Author(s)
      Yutaka Suzuki
    • Organizer
      Human Genome Meeting 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Multi-omics analysis of cancer cells2017

    • Author(s)
      Yutaka Suzuki
    • Organizer
      The 1st International Symposium for Trans-Omics
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Book] シングルセルゲノミクス2019

    • Author(s)
      渡辺 亮、鈴木 穣
    • Total Pages
      219
    • Publisher
      羊土社
    • ISBN
      9784758103831
    • Related Report
      2019 Annual Research Report
  • [Book] “Single Molecule and Single Cell Sequencing”, Advances in Experimental Medicine and Biology2019

    • Author(s)
      Yutaka Suzuki (Editor)
    • Total Pages
      150
    • Publisher
      Springer, Singapore
    • ISBN
      9789811360367
    • Related Report
      2018 Annual Research Report
  • [Remarks] 記者発表:がんに存在する異常なメッセンジャーRNAの全長構造を同定

    • URL

      https://www.k.u-tokyo.ac.jp/information/category/press/3880.html

    • Related Report
      2020 Annual Research Report

URL: 

Published: 2017-07-04   Modified: 2023-01-30  

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