| Budget Amount *help |
¥104,650,000 (Direct Cost: ¥80,500,000、Indirect Cost: ¥24,150,000)
Fiscal Year 2021: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2020: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2019: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2018: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2017: ¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
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| Outline of Final Research Achievements |
To elucidate the cellular diversity in intestinal tissue and their alteration induced by driver mutations found in human colorectal cancers, we established a series of conditional knockout mice and their organoids. Single cell expression analysis of organoids followed by informatics analyses suggested the cellular diversity in norma intestine and their alterations by the driver mutations. Notably, they alters not only cellular diversity in intestinal epithelial cells, but also affects stromal cells in their microenvironment. These alterations were histologically validated in mouse models. Overall, this study revealed the cellular heterogeniety of intestinal tissues in normal and mutant tissues,, and provide the clue to develop the treatment of intestinal tumor by targeting interaction between intestinal epithelia cells and microenvironment.
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