| Budget Amount *help |
¥208,520,000 (Direct Cost: ¥160,400,000、Indirect Cost: ¥48,120,000)
Fiscal Year 2021: ¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
Fiscal Year 2020: ¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
Fiscal Year 2019: ¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
Fiscal Year 2018: ¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
Fiscal Year 2017: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
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| Outline of Final Research Achievements |
In line with the initial research proposal aiming to explore the "Organic chemistry in multimolecular crowding conditions", we developed new organic chemical reactions that can work in live cells, and clarified their reaction characteristics (reaction kinetics, reaction selectivity for functional groups, and stability in crowding conditions). Specifically, we conducted (1) development of novel ligand-directed chemistry and its application to chemical labeling of receptor proteins and design of covalent inhibitors for disease-associated proteins, (2) development of novel reaction systems that allow the specific labeling of biomolecules (lipids and proteins) in particular environments or organelles in live cells and application of this techniques to the imaging analysis of intracellular dynamics of biomolecules under live cell conditions. (3) design and biomimetic construction of artificial molecular crowding systems by employing the supramolecular multi-component composite systems.
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